Origin IGS: attctcaacttattatctaggttgctatattttcttaattaccacttacgatcggctgcttggcgaaggtggcggctcatgaatgtttgtacgggcgataaactctcaaactctgcacacactttctgctagccaaaccgccacttgcaccaaaccgcttgttgtaagctgtgcattcattgctaatgtgtttgttttca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaaaacaaacacattagcaatgaatgcacagcttacaacaagcggtttggtgcaagtggcggtttggctagcagaaagtgtgtgcagagtttgagagtttatcgcccgtacaaacattcatgagccgccaccttcgccaagcagccgatcgtaagtggtaattaagaaaatatagcaacctagataataagttgagaat Mask Tandem Repeat Region ================================================ attctcaacttattatctaggttgctatattttcttaattaccacttacgatcggctgcttggcgaaggtggcggctcatgaatgtttgtacgggcgataaactctcaaactctgcacacactttctgctagccaaaccgccacttgcaccaaaccgcttgttgtaagctgtgcattcattgctaatgtgtttgttttca Find is-nt database================================================ Query_seq: TF1693:TF1694|TF1693:TF1694:conserved hypothetical protein; possible thiopurine S-methyltransferase (TPMT) family protein:conserved hypothetical protein:->->:1812433..1812632 200 attctcaacttattatctaggttgctatattttcttaattaccacttacgatcggctgcttggcgaaggtggcggctcatgaatgtttgtacgggcgataaactctcaaactctgcacacactttctgctagccaaaccgccacttgcaccaaaccgcttgttgtaagctgtgcattcattgctaatgtgtttgttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1693:TF1694|TF1693:TF1694:conserved hypothetical protein; possible thiopurine S-methyltransferase (TPMT) family protein:conserved hypothetical protein:->->:1812433..1812632 200 attctcaacttattatctaggttgctatattttcttaattaccacttacgatcggctgcttggcgaaggtggcggctcatgaatgtttgtacgggcgataaactctcaaactctgcacacactttctgctagccaaaccgccacttgcaccaaaccgcttgttgtaagctgtgcattcattgctaatgtgtttgttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1693:TF1694|TF1693:TF1694:conserved hypothetical protein; possible thiopurine S-methyltransferase (TPMT) family protein:conserved hypothetical protein:->->:1812433..1812632 200 attctcaacttattatctaggttgctatattttcttaattaccacttacgatcggctgcttggcgaaggtggcggctcatgaatgtttgtacgggcgataaactctcaaactctgcacacactttctgctagccaaaccgccacttgcaccaaaccgcttgttgtaagctgtgcattcattgctaatgtgtttgttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attctcaacttattatctaggttgctatattttcttaattaccacttacgatcggctgcttggcgaaggtggcggctcatgaatgtttgtacgggcgataaactctcaaactctgcacacactttctgctagccaaaccgccacttgcaccaaaccgcttgttgtaagctgtgcattcattgctaatgtgtttgttttca Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 1 End: 129 M L N L L S R L L Y F L N Y H L R S A A W R R W R L M N V C T G D K L S N S A H T F C ....................................................................... Protein_Len: 39 Strand: + Start: 83 End: 199 .................................................................................. M F V R A I N S Q T L H T L S A S Q T A T C T K P L V V S C A F I A N V F V F . Protein_Len: 36 Strand: - Start: 80 End: 187 ............................................................................... S H K Y P R Y V R L S Q V C K R S A L G G S A G F R K N Y A T C E N S M ............. attctcaacttattatctaggttgctatattttcttaattaccacttacgatcggctgcttggcgaaggtggcggctcatgaatgtttgtacgggcgataaactctcaaactctgcacacactttctgctagccaaaccgccacttgcaccaaaccgcttgttgtaagctgtgcattcattgctaatgtgtttgttttca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attctcaacttattatctaggttgctatattttcttaattaccacttacgatcggctgcttggcgaaggtggcggctcatgaatgtttgtacgggcgataaactctcaaactctgcacacactttctgctagccaaaccgccacttgcaccaaaccgcttgttgtaagctgtgcattcattgctaatgtgtttgttttca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1693:TF1694|TF1693:TF1694:conserved hypothetical protein; possible thiopurine S-methyltransferase (TPMT) family protein:conserved hypothetical protein:->->:1812433..1812632 200 attctcaacttattatctaggttgctatattttcttaattaccacttacgatcggctgcttggcgaaggtggcggctcatgaatgtttgtacgggcgataaactctcaaactctgcacacactttctgctagccaaaccgccacttgcaccaaaccgcttgttgtaagctgtgcattcattgctaatgtgtttgttttca Intra-Species Hit: Count: 1 Min: 1 Max: 200 Len: 200 Subject: tfor_TF1693_TF1694|conserved hypothetical protein; possible thiopurine S-methyltransferase (TPMT) family protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1812433,1812632]|200 HSP 1 e-value: 1.0E-109 bit: 396.0 Len: 200 Query Start:1 Query End:200 Subject Strand: POSITIVE Subject Start: 1 Subject End: 200 attctcaacttattatctaggttgctatattttcttaattaccacttacgatcggctgcttggcgaaggtggcggctcatgaatgtttgtacgggcgataaactctcaaactctgcacacactttctgctagccaaaccgccacttgcaccaaaccgcttgttgtaagctgtgcattcattgctaatgtgtttgttttca attctcaacttattatctaggttgctatattttcttaattaccacttacgatcggctgcttggcgaaggtggcggctcatgaatgtttgtacgggcgataaactctcaaactctgcacacactttctgctagccaaaccgccacttgcaccaaaccgcttgttgtaagctgtgcattcattgctaatgtgtttgttttca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUCUCAACUUAUUAUCUAGGUUGCUAUAUUUUCUUAAUUACCACUUACGAUCGGCUGCUUGGCGAAGGUGGCGGCUCAUGAAUGUUUGUACGGGCGAUAAACUCUCAAACUCUGCACACACUUUCUGCUAGCCAAACCGCCACUUGCACCAAACCGCUUGUUGUAAGCUGUGCAUUCAUUGCUAAUGUGUUUGUUUUCA .....(((((((.....))))))).((((((...........((((((((((.(((.(.((((.(.(((((((((....(((..((((((.(....)))))))..)))......(((.........)))........))))))))).).)))).).))).)))))))).)).(((.....))).)))))).......... (-48.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAAACAAACACAUUAGCAAUGAAUGCACAGCUUACAACAAGCGGUUUGGUGCAAGUGGCGGUUUGGCUAGCAGAAAGUGUGUGCAGAGUUUGAGAGUUUAUCGCCCGUACAAACAUUCAUGAGCCGCCACCUUCGCCAAGCAGCCGAUCGUAAGUGGUAAUUAAGAAAAUAUAGCAACCUAGAUAAUAAGUUGAGAAU ..............(((((..(((.(((...((((((.....((.((((((((...((((((((((.(....).(((.((.(((((.(.((..((.......))))).))))).)).)))..))))))))))...)))))))).)).....)))))).))).)))........(((....)))........))))).... (-50.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 521 99 51 57 2 tfor:TF1479|5end_ABC_transporter,_permease_component_1572241..1572471_POSITIVE 513 100 53 231 172 tfor:TF2016|5end_hypothetical_protein_2172948..2173178_POSITIVE 448 94 52 68 18 tfor:TF1586|5end_hypothetical_protein_1690971..1691201_POSITIVE 438 99 55 188 143 tfor:TF2444|5end_regulatory_protein_RecX_2620957..2621187_POSITIVE 429 96 54 179 123 tfor:TF2306|5end_hypothetical_protein_2466674..2466904_POSITIVE 415 100 52 64 13 tfor:TF1401|5end_Holliday_junction_DNA_helicase_1476502..1476732_POSITIVE 414 85 49 44 10 tfor:TF0700|5end_possible_lipoprotein_742649..742879_POSITIVE 401 97 53 61 13 tfor:TF2257|5end_transfer_region-related_protein,_TraJ_2428541..2428771_POSITIVE 400 86 45 72 34 tfor:TF3040|5end_inosine-5'-monophosphate_dehydrogenase_3267655..3267885_POSITIVE 400 76 40 222 174 tfor:TF2822|5end_arginine_repressor_3011793..3012023_POSITIVE 400 97 51 130 90 tfor:TF2754|5end_hypothetical_protein_2940446..2940676_POSITIVE 400 90 41 101 48 tfor:TF1797|5end_SNF_family_transporter_1937136..1937366_POSITIVE 395 97 52 177 121 tfor:TF0969|5end_oxidoreductase_1019550..1019780_POSITIVE 385 97 53 184 137 tfor:TF2207|5end_exo-alpha-sialidase_(neuraminidase)_2380907..2381137_POSITIVE 382 100 55 211 159 tfor:TF1848|5end_conserved_hypothetical_protein_1997589..1997819_POSITIVE 380 97 51 109 56 tfor:TF2115|5end_conserved_hypothetical_protein;_possible_carboxylesterase_2284786..2285016_POSITIVE 378 98 52 61 24 tfor:TF2912|5end_hypothetical_protein_3106508..3106738_POSITIVE 375 76 47 215 180 tfor:TF2520|5end_conserved_hypothetical_protein_2698917..2699147_POSITIVE 374 98 51 86 44 tfor:TF1067|5end_conserved_hypothetical_protein_1136867..1137097_POSITIVE 369 81 42 229 184 tfor:TF2397|5end_magnesium_and_cobalt_transport_protein_2568545..2568775_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 415 100 52 55 4 tfor:TF1400|3end_polysaccharide_biosynthesis_protein;_membrane_protein_1476573..1476723_POSITIVE 414 85 49 60 26 tfor:TF0699|3end_pyridoxal_phosphate_biosynthetic_protein_742745..742895_POSITIVE 401 97 53 54 6 tfor:TF2256|3end_transfer_region-related_protein,_TraI_2428614..2428764_POSITIVE 400 97 51 52 12 tfor:TF2753|3end_zinc_protease_2940448..2940598_POSITIVE 400 96 52 146 104 tfor:TF2092|3end_ribonuclease_III_2261823..2261973_POSITIVE 400 90 41 101 48 tfor:TF1796|3end_ABC_transporter,_ATP-binding_protein_1937216..1937366_POSITIVE 381 97 52 149 99 tfor:TF0968|3end_hypothetical_protein_1019608..1019758_POSITIVE 378 98 52 113 76 tfor:TF2911|3end_hypothetical_protein_3106640..3106790_POSITIVE 377 86 49 34 1 tfor:TF3039|3end_ABC_transporter,_ATP-binding_protein_3267697..3267847_POSITIVE 370 110 68 42 3 tfor:TF1478|3end_membrane_fusion_efflux_protein_1572296..1572446_POSITIVE 367 171 131 82 38 tfor:TF2015|3end_N6-adenine-specific_DNA_methylase_2173020..2173170_POSITIVE 363 100 53 99 42 tfor:TF2848|3end_cobalamin_biosynthesis_protein_3037389..3037539_POSITIVE 361 95 54 138 82 tfor:TF0880|3end_conserved_hypothetical_protein_934371..934521_POSITIVE 360 160 111 63 11 tfor:TF2703|3end_conserved_hypothetical_protein_2876317..2876467_POSITIVE 359 98 52 86 26 tfor:TF1403|3end_hypothetical_protein_1478025..1478175_POSITIVE 356 85 45 72 13 tfor:TF1165|3end_conserved_hypothetical_protein_1245921..1246071_POSITIVE 355 99 51 146 106 tfor:TF0982|3end_conserved_hypothetical_protein_1039474..1039624_POSITIVE 353 99 51 76 20 tfor:TF2829|3end_conserved_hypothetical_protein_3020661..3020811_POSITIVE 348 97 51 136 95 tfor:TF2454|3end_D-alanyl-D-alanine_dipeptidase_2632025..2632175_POSITIVE 347 97 52 46 5 tfor:TF1002|3end_replicative_DNA_helicase_1058232..1058382_POSITIVE