Origin IGS: taatcgacaaataagtactaaactgtttttgttccaacagatttatacgaaagcgcaaattccatttgatataaccgcttataccaactactggacttcatcgttgctctccagtttacctgattcaaataccgtgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacacggtatttgaatcaggtaaactggagagcaacgatgaagtccagtagttggtataagcggttatatcaaatggaatttgcgctttcgtataaatctgttggaacaaaaacagtttagtacttatttgtcgatta Mask Tandem Repeat Region ================================================ taatcgacaaataagtactaaactgtttttgttccaacagatttatacgaaagcgcaaattccatttgatataaccgcttataccaactactggacttcatcgttgctctccagtttacctgattcaaataccgtgtta Find is-nt database================================================ Query_seq: TF1692:TF1693|TF1692:TF1693:conserved hypothetical protein; possible mobilizable transposon, TnpC:conserved hypothetical protein; possible thiopurine S-methyltransferase (TPMT) family protein:->->:1811493..1811631 139 taatcgacaaataagtactaaactgtttttgttccaacagatttatacgaaagcgcaaattccatttgatataaccgcttataccaactactggacttcatcgttgctctccagtttacctgattcaaataccgtgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1692:TF1693|TF1692:TF1693:conserved hypothetical protein; possible mobilizable transposon, TnpC:conserved hypothetical protein; possible thiopurine S-methyltransferase (TPMT) family protein:->->:1811493..1811631 139 taatcgacaaataagtactaaactgtttttgttccaacagatttatacgaaagcgcaaattccatttgatataaccgcttataccaactactggacttcatcgttgctctccagtttacctgattcaaataccgtgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1692:TF1693|TF1692:TF1693:conserved hypothetical protein; possible mobilizable transposon, TnpC:conserved hypothetical protein; possible thiopurine S-methyltransferase (TPMT) family protein:->->:1811493..1811631 139 taatcgacaaataagtactaaactgtttttgttccaacagatttatacgaaagcgcaaattccatttgatataaccgcttataccaactactggacttcatcgttgctctccagtttacctgattcaaataccgtgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatcgacaaataagtactaaactgtttttgttccaacagatttatacgaaagcgcaaattccatttgatataaccgcttataccaactactggacttcatcgttgctctccagtttacctgattcaaataccgtgtta Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 11 End: 139 .......... M S T K L F L F Q Q I Y T K A Q I P F D I T A Y T N Y W T S S L L S S L P D S N T V L Protein_Len: 36 Strand: - Start: 21 End: 128 .................... V T K T G V S K Y S L A F E M Q Y L R K Y W S S S K M T A R W N V Q N M ........... taatcgacaaataagtactaaactgtttttgttccaacagatttatacgaaagcgcaaattccatttgatataaccgcttataccaactactggacttcatcgttgctctccagtttacctgattcaaataccgtgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatcgacaaataagtactaaactgtttttgttccaacagatttatacgaaagcgcaaattccatttgatataaccgcttataccaactactggacttcatcgttgctctccagtttacctgattcaaataccgtgtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1692:TF1693|TF1692:TF1693:conserved hypothetical protein; possible mobilizable transposon, TnpC:conserved hypothetical protein; possible thiopurine S-methyltransferase (TPMT) family protein:->->:1811493..1811631 139 taatcgacaaataagtactaaactgtttttgttccaacagatttatacgaaagcgcaaattccatttgatataaccgcttataccaactactggacttcatcgttgctctccagtttacctgattcaaataccgtgtta Intra-Species Hit: Count: 1 Min: 1 Max: 139 Len: 139 Subject: tfor_TF1692_TF1693|conserved hypothetical protein; possible mobilizable transposon, TnpC:conserved hypothetical protein; possible thiopurine S-methyltransferase (TPMT) family protein|POSITIVE:POSITIVE|[1811493,1811631]|139 HSP 1 e-value: 2.0E-73 bit: 276.0 Len: 139 Query Start:1 Query End:139 Subject Strand: POSITIVE Subject Start: 1 Subject End: 139 taatcgacaaataagtactaaactgtttttgttccaacagatttatacgaaagcgcaaattccatttgatataaccgcttataccaactactggacttcatcgttgctctccagtttacctgattcaaataccgtgtta taatcgacaaataagtactaaactgtttttgttccaacagatttatacgaaagcgcaaattccatttgatataaccgcttataccaactactggacttcatcgttgctctccagtttacctgattcaaataccgtgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCGACAAAUAAGUACUAAACUGUUUUUGUUCCAACAGAUUUAUACGAAAGCGCAAAUUCCAUUUGAUAUAACCGCUUAUACCAACUACUGGACUUCAUCGUUGCUCUCCAGUUUACCUGAUUCAAAUACCGUGUUA ..............(((.(((((((((........))))).)))))))...(((((.........(((.(((((....))))).)))..((((((...((....))...))))))..................))))). (-19.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACACGGUAUUUGAAUCAGGUAAACUGGAGAGCAACGAUGAAGUCCAGUAGUUGGUAUAAGCGGUUAUAUCAAAUGGAAUUUGCGCUUUCGUAUAAAUCUGUUGGAACAAAAACAGUUUAGUACUUAUUUGUCGAUUA ...........((((..(((((((....((((((..(((.....((((....(((((((((....))))))))).))))..))).))))))...((((.(((((........)))))))))....)))))))))))... (-28.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 283 120 75 220 178 tfor:TF2789|5end_enoyl-[acyl-carrier-protein]_reductase_2972431..2972661_POSITIVE 278 107 66 51 5 tfor:TF0906|5end_conserved_hypothetical_protein_949675..949905_POSITIVE 277 99 55 62 22 tfor:TF1240|5end_conserved_hypothetical_protein_1324304..1324534_POSITIVE 271 128 84 166 123 tfor:TF3018|5end_polyphosphate_kinase_3240122..3240352_POSITIVE 263 123 82 33 2 tfor:TF0763|5end_chromate_transport_protein_809675..809905_POSITIVE 263 112 76 210 177 tfor:TF0278|5end_conserved_hypothetical_protein_305570..305800_POSITIVE 262 125 95 125 92 tfor:TF1873|5end_possible_hemolysin_secretion_protein_2026745..2026975_POSITIVE 262 124 91 162 121 tfor:TF1852|5end_prtQ_protein_2004643..2004873_POSITIVE 262 125 95 125 92 tfor:TF1131|5end_membrane-fusion_protein;_possible_hemolysin_secretion_protein_1207611..1207841_POSITIVE 261 107 62 226 162 tfor:TF2332|5end_signal_peptidase_I_2491694..2491924_POSITIVE 259 119 73 140 96 tfor:TF1710|5end_possible_cell_filamentation_protein_1829679..1829909_POSITIVE 255 118 75 41 2 tfor:TF2932|5end_capsule_biosynthesis_protein_3135514..3135744_POSITIVE 255 96 55 97 47 tfor:TF2883|5end_peptidyl-prolyl_cis-trans_isomerase_3074321..3074551_POSITIVE 247 116 75 114 58 tfor:TF0257|5end_hypothetical_protein_287610..287840_POSITIVE 243 122 88 47 13 tfor:TF2833|5end_lipid_A_disaccharide_synthase_3023973..3024203_POSITIVE 243 113 75 50 10 tfor:TF0496|5end_conserved_hypothetical_protein_522332..522562_POSITIVE 241 80 31 174 124 tfor:TF1547|5end_conserved_hypothetical_protein_1651459..1651689_POSITIVE 240 120 75 221 176 tfor:TF2904|5end_ATP-dependent__helicase,_possible_UvrD/REP_helicase_domain_protein_3098903..3099133_POSITIVE 240 80 38 226 177 tfor:TF1929|5end_conserved_hypothetical_protein_2076049..2076279_POSITIVE 239 103 75 224 194 tfor:TF2443|5end_amidophosphoribosyl-transferase_2620287..2620517_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 282 127 88 137 88 tfor:TF0886|3end_conjugative_transposon_protein_fragment_938152..938302_POSITIVE 263 123 82 33 2 tfor:TF0762|3end_chromate_transport_protein_809755..809905_POSITIVE 262 125 95 111 78 tfor:TF1872|3end_ABC_transporter,_ATP-binding/permease_component_2026811..2026961_POSITIVE 262 124 91 120 79 tfor:TF1851|3end_methionine_synthase_I_2004681..2004831_POSITIVE 262 125 95 111 78 tfor:TF1130|3end_ABC_transporter_ATP-binding_protein/permease_component_1207677..1207827_POSITIVE 259 119 73 91 47 tfor:TF1709|3end_conserved_hypothetical_protein_1829710..1829860_POSITIVE 255 96 55 97 47 tfor:TF2882|3end_conserved_hypothetical_protein_3074401..3074551_POSITIVE 254 120 75 66 1 tfor:TF1010|3end_penicillin_binding_protein_2_1066734..1066884_POSITIVE 243 120 75 147 92 tfor:TF2795|3end_hypothetical_protein_2980909..2981059_POSITIVE 242 120 77 36 2 tfor:TF2552|3end_50S_ribosomal_protein_L3_2735452..2735602_POSITIVE 239 103 75 128 98 tfor:TF2442|3end_heat_shock_protein_60_2620271..2620421_POSITIVE 238 80 45 139 106 tfor:TF1645|3end_conserved_hypothetical_protein_1756970..1757120_POSITIVE 237 117 76 129 71 tfor:TF1042|3end_possible_surface_antigen_with_leucine-rich_repeat_1105295..1105445_POSITIVE 237 115 76 45 6 tfor:TF0288|3end_conserved_hypothetical_protein_318099..318249_POSITIVE 235 120 75 56 5 tfor:TF3118|3end_conserved_hypothetical_protein_3352824..3352974_POSITIVE 234 127 97 40 9 tfor:TF0885|3end_conjugative_transposon_protein_fragment_938055..938205_POSITIVE 232 116 76 41 1 tfor:TF3148|3end_hypoxanthine-guanine_phosphoribosyltransferase_3384271..3384421_POSITIVE 231 113 75 52 7 tfor:TF1870|3end_conserved_hypothetical_protein;_possible_transposase_2024243..2024393_POSITIVE 227 115 73 140 99 tfor:TF2389|3end_conserved_hypothetical_protein_2560548..2560698_POSITIVE 227 78 33 104 58 tfor:TF1761|3end_hypothetical_protein_1899579..1899729_POSITIVE