Origin IGS: taaaaacaaatttttattcgaaccaaatcttttcgaatcttttttgaatcttttttctcaaaccctcatatcaaacaatatggaaaatccgaaaaaagaaaaacccaaaaacaaatcctttcttatcacgctttgcagcgggctcccctctcgaaagcgggtgcaaaagtacggaatatcttctttccctacaaatattttatgacctttttttgaagaaaaagcgcaaattgttaagaaacaactatttataagtaaacaaaaactttacagcgctacattcctctggatataaaataatgtatcgcgcacattagataacaaagcagatattctctcttacccgacttataagagcgaactttatcgtaatattcgatctgcctttcaacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagcccttataaacctcgcagaggcggtgagttgttgatttgttgacatgttgatttgttggctgattactgaatactgaccgctaacgattatatcgggcttgcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccattgacaattcaccattaataaataggaatacataccgttatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taataacggtatgtattcctatttattaatggtgaattgtcaatggttaattgtcatgccgggtacatgattcggcgtgtaacacgcataaccattacccgaaagggctgcaagcccgatataatcgttagcggtcagtattcagtaatcagccaacaaatcaacatgtcaacaaatcaacaactcaccgcctctgcgaggtttataagggctgaaggcctgaattaaattcattcttattggttctattgcggaatctgggttgaaaggcagatcgaatattacgataaagttcgctcttataagtcgggtaagagagaatatctgctttgttatctaatgtgcgcgatacattattttatatccagaggaatgtagcgctgtaaagtttttgtttacttataaatagttgtttcttaacaatttgcgctttttcttcaaaaaaaggtcataaaatatttgtagggaaagaagatattccgtacttttgcacccgctttcgagaggggagcccgctgcaaagcgtgataagaaaggatttgtttttgggtttttcttttttcggattttccatattgtttgatatgagggtttgagaaaaaagattcaaaaaagattcgaaaagatttggttcgaataaaaatttgttttta Mask Tandem Repeat Region ================================================ taaaaacaaatttttattcgaaccaaatcttttcgaatcttttttgaatcttttttctcaaaccctcatatcaaacaatatggaaaatccgaaaaaagaaaaacccaaaaacaaatcctttcttatcacgctttgcagcgggctcccctctcgaaagcgggtgcaaaagtacggaatatcttctttccctacaaatattttatgacctttttttgaagaaaaagcgcaaattgttaagaaacaactatttataagtaaacaaaaactttacagcgctacattcctctggatataaaataatgtatcgcgcacattagataacaaagcagatattctctcttacccgacttataagagcgaactttatcgtaatattcgatctgcctttcaacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagcccttataaacctcgcagaggcggtgagttgttgatttgttgacatgttgatttgttggctgattactgaatactgaccgctaacgattatatcgggcttgcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccattgacaattcaccattaataaataggaatacataccgttatta Find is-nt database================================================ Query_seq: TF1654:TF1655|TF1654:TF1655:hypothetical protein:long-chain-fatty-acid-CoA ligase:->->:1768766..1769418 653 taaaaacaaatttttattcgaaccaaatcttttcgaatcttttttgaatcttttttctcaaaccctcatatcaaacaatatggaaaatccgaaaaaagaaaaacccaaaaacaaatcctttcttatcacgctttgcagcgggctcccctctcgaaagcgggtgcaaaagtacggaatatcttctttccctacaaatattttatgacctttttttgaagaaaaagcgcaaattgttaagaaacaactatttataagtaaacaaaaactttacagcgctacattcctctggatataaaataatgtatcgcgcacattagataacaaagcagatattctctcttacccgacttataagagcgaactttatcgtaatattcgatctgcctttcaacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagcccttataaacctcgcagaggcggtgagttgttgatttgttgacatgttgatttgttggctgattactgaatactgaccgctaacgattatatcgggcttgcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccattgacaattcaccattaataaataggaatacataccgttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1654:TF1655|TF1654:TF1655:hypothetical protein:long-chain-fatty-acid-CoA ligase:->->:1768766..1769418 653 taaaaacaaatttttattcgaaccaaatcttttcgaatcttttttgaatcttttttctcaaaccctcatatcaaacaatatggaaaatccgaaaaaagaaaaacccaaaaacaaatcctttcttatcacgctttgcagcgggctcccctctcgaaagcgggtgcaaaagtacggaatatcttctttccctacaaatattttatgacctttttttgaagaaaaagcgcaaattgttaagaaacaactatttataagtaaacaaaaactttacagcgctacattcctctggatataaaataatgtatcgcgcacattagataacaaagcagatattctctcttacccgacttataagagcgaactttatcgtaatattcgatctgcctttcaacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagcccttataaacctcgcagaggcggtgagttgttgatttgttgacatgttgatttgttggctgattactgaatactgaccgctaacgattatatcgggcttgcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccattgacaattcaccattaataaataggaatacataccgttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1654:TF1655|TF1654:TF1655:hypothetical protein:long-chain-fatty-acid-CoA ligase:->->:1768766..1769418 653 taaaaacaaatttttattcgaaccaaatcttttcgaatcttttttgaatcttttttctcaaaccctcatatcaaacaatatggaaaatccgaaaaaagaaaaacccaaaaacaaatcctttcttatcacgctttgcagcgggctcccctctcgaaagcgggtgcaaaagtacggaatatcttctttccctacaaatattttatgacctttttttgaagaaaaagcgcaaattgttaagaaacaactatttataagtaaacaaaaactttacagcgctacattcctctggatataaaataatgtatcgcgcacattagataacaaagcagatattctctcttacccgacttataagagcgaactttatcgtaatattcgatctgcctttcaacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagcccttataaacctcgcagaggcggtgagttgttgatttgttgacatgttgatttgttggctgattactgaatactgaccgctaacgattatatcgggcttgcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccattgacaattcaccattaataaataggaatacataccgttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaaacaaatttttattcgaaccaaatcttttcgaatcttttttgaatcttttttctcaaaccctcatatcaaacaatatggaaaatccgaaaaaagaaaaacccaaaaacaaatcctttcttatcacgctttgcagcgggctcccctctcgaaagcgggtgcaaaagtacggaatatcttctttccctacaaatattttatgacctttttttgaagaaaaagcgcaaattgttaagaaacaactatttataagtaaacaaaaactttacagcgctacattcctctggatataaaataatgtatcgcgcacattagataacaaagcagatattctctcttacccgacttataagagcgaactttatcgtaatattcgatctgcctttcaacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagcccttataaacctcgcagaggcggtgagttgttgatttgttgacatgttgatttgttggctgattactgaatactgaccgctaacgattatatcgggcttgcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccattgacaattcaccattaataaataggaatacataccgttatta Predict ORF larger than 30AA ================================================ Protein_Len: 53 Strand: + Start: 44 End: 202 ........................................... M N L F S Q T L I S N N M E N P K K E K P K N K S F L I T L C S G L P S R K R V Q K Y G I S S F P T N I L ................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 10 End: 213 ......... M F I R T K S F R I F F E S F F S N P H I K Q Y G K S E K R K T Q K Q I L S Y H A L Q R A P L S K A G A K V R N I F F P ........................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 48 End: 251 ............................................... M F F L K P S Y Q T I W K I R K K K N P K T N P F L S R F A A G S P L E S G C K S T E Y L L S L Q I F Y D L F L K K K R .................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 39 Strand: + Start: 332 End: 448 ........................................................................................................................................................................................................................................................................................................................................... M L S Y P T Y K S E L Y R N I R S A F Q P R F R N R T N K N E F N S G L Q P L ............................................................................................................................................................................................................. Protein_Len: 55 Strand: + Start: 318 End: 482 ............................................................................................................................................................................................................................................................................................................................. M T K Q I F S L T R L I R A N F I V I F D L P F N P D S A I E P I R M N L I Q A F S P Y K P R R G G E L L I C ........................................................................................................................................................................... Protein_Len: 33 Strand: + Start: 513 End: 611 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M L T A N D Y I G L A A L S G N G Y A C Y T P N H V P G M T I N H .......................................... Protein_Len: 60 Strand: - Start: 429 End: 629 ............................................................................................................................................................................................................................................................................................................................................................................................................................................ F R A S A T L Q Q N T S M N I Q Q S I V S Y Q G S V I I D P K C G K R T I T I R T V R R I M Y G A H C N V M S L E G N M ........................ Protein_Len: 38 Strand: - Start: 317 End: 430 ............................................................................................................................................................................................................................................................................................................................ I V F C I N E R V R S I L A F K I T I N S R G K L G S E A I S G I L I F K M ............................................................................................................................................................................................................................... Protein_Len: 49 Strand: - Start: 279 End: 425 ...................................................................................................................................................................................................................................................................................... M G R S I F Y H I A C M L Y C L L Y E R K G S K Y S R V K D Y Y E I Q R E V W I G C Y F W Y S H M .................................................................................................................................................................................................................................... Protein_Len: 41 Strand: - Start: 74 End: 196 ......................................................................... V I H F I R F F F F G F V F G K K D R K A A P E G R S L P H L L V S Y R R E R C M ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 61 End: 252 ............................................................ F L I S F G F F S F G L F L D K R I V S Q L P S G E R F R T C F Y P I D E K G V F I K H G K K S S F A C I T L F C S N M ................................................................................................................................................................................................................................................................................................................................................................................................................. taaaaacaaatttttattcgaaccaaatcttttcgaatcttttttgaatcttttttctcaaaccctcatatcaaacaatatggaaaatccgaaaaaagaaaaacccaaaaacaaatcctttcttatcacgctttgcagcgggctcccctctcgaaagcgggtgcaaaagtacggaatatcttctttccctacaaatattttatgacctttttttgaagaaaaagcgcaaattgttaagaaacaactatttataagtaaacaaaaactttacagcgctacattcctctggatataaaataatgtatcgcgcacattagataacaaagcagatattctctcttacccgacttataagagcgaactttatcgtaatattcgatctgcctttcaacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagcccttataaacctcgcagaggcggtgagttgttgatttgttgacatgttgatttgttggctgattactgaatactgaccgctaacgattatatcgggcttgcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccattgacaattcaccattaataaataggaatacataccgttatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaaacaaatttttattcgaaccaaatcttttcgaatcttttttgaatcttttttctcaaaccctcatatcaaacaatatggaaaatccgaaaaaagaaaaacccaaaaacaaatcctttcttatcacgctttgcagcgggctcccctctcgaaagcgggtgcaaaagtacggaatatcttctttccctacaaatattttatgacctttttttgaagaaaaagcgcaaattgttaagaaacaactatttataagtaaacaaaaactttacagcgctacattcctctggatataaaataatgtatcgcgcacattagataacaaagcagatattctctcttacccgacttataagagcgaactttatcgtaatattcgatctgcctttcaacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagcccttataaacctcgcagaggcggtgagttgttgatttgttgacatgttgatttgttggctgattactgaatactgaccgctaacgattatatcgggcttgcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccattgacaattcaccattaataaataggaatacataccgttatta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 60 HP_score: -2.5 Tail_Score: -4.52589 Start: 298 End: 316 Full_Region: atctgctttgttatc taatgtg cgcga tacatta ttttatatccagagg .........................................................................................................................................................................................................................................................................................................taatgtgcgcgatacatta................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 75 HP_score: -9.1 Tail_Score: -3.6284 Start: 537 End: 549 Full_Region: GCTAACGATTATATC GGGCT TGC AGCCC TTTCGGGTAATGGTT ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GGGCTTGCAGCCC........................................................................................................ Find igs database================================================ Query_seq: TF1654:TF1655|TF1654:TF1655:hypothetical protein:long-chain-fatty-acid-CoA ligase:->->:1768766..1769418 653 taaaaacaaatttttattcgaaccaaatcttttcgaatcttttttgaatcttttttctcaaaccctcatatcaaacaatatggaaaatccgaaaaaagaaaaacccaaaaacaaatcctttcttatcacgctttgcagcgggctcccctctcgaaagcgggtgcaaaagtacggaatatcttctttccctacaaatattttatgacctttttttgaagaaaaagcgcaaattgttaagaaacaactatttataagtaaacaaaaactttacagcgctacattcctctggatataaaataatgtatcgcgcacattagataacaaagcagatattctctcttacccgacttataagagcgaactttatcgtaatattcgatctgcctttcaacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagcccttataaacctcgcagaggcggtgagttgttgatttgttgacatgttgatttgttggctgattactgaatactgaccgctaacgattatatcgggcttgcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccattgacaattcaccattaataaataggaatacataccgttatta Intra-Species Hit: Count: 11 Min: 116 Max: 653 Len: 538 Subject: tfor_TF1654_TF1655|hypothetical protein:long-chain-fatty-acid-CoA ligase|POSITIVE:POSITIVE|[1768766,1769418]|653 HSP 1 e-value: 0.0 bit: 1025.0 Len: 538 Query Start:116 Query End:653 Subject Strand: POSITIVE Subject Start: 116 Subject End: 653 ...................................................................................................................tcctttcttatcacgctttgcagcgggctcccctctcgaaagcgggtgcaaaagtacggaatatcttctttccctacaaatattttatgaccnnnnnnngaagaaaaagcgcaaattgttaagaaacaactatttataagtaaacaaaaactttacagcgctacattcctctggatataaaataatgtatcgcgcacattagataacaaagcagatattctctcttacccgacttataagagcgaactttatcgtaatattcgatctgcctttcaacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagcccttataaacctcgcagaggcggtgagttgttgatttgttgacatgttgatttgttggctgattactgaatactgaccgctaacgattatatcgggcttgcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccattgacaattcaccattaataaataggaatacataccgttatta ...................................................................................................................tcctttcttatcacgctttgcagcgggctcccctctcgaaagcgggtgcaaaagtacggaatatcttctttccctacaaatattttatgacctttttttgaagaaaaagcgcaaattgttaagaaacaactatttataagtaaacaaaaactttacagcgctacattcctctggatataaaataatgtatcgcgcacattagataacaaagcagatattctctcttacccgacttataagagcgaactttatcgtaatattcgatctgcctttcaacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagcccttataaacctcgcagaggcggtgagttgttgatttgttgacatgttgatttgttggctgattactgaatactgaccgctaacgattatatcgggcttgcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccattgacaattcaccattaataaataggaatacataccgttatta Subject: tfor_TF0179_TF0180|pyrophosphate-energized proton pump:carboxynorspermidine decarboxylase|POSITIVE:POSITIVE|[190752,191406]|655 HSP 1 e-value: 5.0E-56 bit: 220.0 Len: 165 Query Start:472 Query End:636 Subject Strand: NEGATIVE Subject Start: 284 Subject End: 441 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgttgatttgttgacatgttgatttgttggctgattactgaatactgaccgctaacgattatatcgggcttgcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccattgacaattcaccattaataaatagg................. .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgttgaattgttgagttgttgagttgttggctgattgctgaacactgaccgctaacgattatatcgggcttgcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatg-------accattgacaattaaccattaataaatagg................. HSP 2 e-value: 8.0E-21 bit: 103.0 Len: 88 Query Start:413 Query End:500 Subject Strand: NEGATIVE Subject Start: 429 Subject End: 516 ............................................................................................................................................................................................................................................................................................................................................................................................................................aataagaatgaatttaattcaggccttcagcccttataaacctcgcagaggcggtgagttgttgatttgttgacatgttgatttgttg......................................................................................................................................................... ............................................................................................................................................................................................................................................................................................................................................................................................................................aataaggatgaatttaattcagaccttcagcccttataaaccccgcagaggcggtgatgcggtgagttgttgacatgttgaattgttg......................................................................................................................................................... Subject: tfor_TF0029_TF0030|hypothetical protein:N-acetylneuraminate lyase|POSITIVE:POSITIVE|[25123,25909]|787 HSP 1 e-value: 1.0E-41 bit: 172.0 Len: 111 Query Start:390 Query End:500 Subject Strand: POSITIVE Subject Start: 12 Subject End: 122 .....................................................................................................................................................................................................................................................................................................................................................................................................aacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagcccttataaacctcgcagaggcggtgagttgttgatttgttgacatgttgatttgttg......................................................................................................................................................... .....................................................................................................................................................................................................................................................................................................................................................................................................aacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagcccttataaacctcgcagaggcggtgaattgttaaattgttgagttgttgattcgttg......................................................................................................................................................... HSP 2 e-value: 1.0E-25 bit: 119.0 Len: 84 Query Start:467 Query End:550 Subject Strand: POSITIVE Subject Start: 105 Subject End: 188 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgagttgttgatttgttgacatgttgatttgttggctgattactgaatactgaccgctaacgattatatcgggcttgcagccct....................................................................................................... ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgagttgttgattcgttgacatgttgatttgttggctgaccgctgaatactgaccgctaacgattatatcaggcttacagccct....................................................................................................... Subject: tfor_TF3104_TF3105|outer membrane protein, TonB dependent receptor:hypothetical protein|POSITIVE:POSITIVE|[3337511,3338207]|697 HSP 1 e-value: 6.0E-40 bit: 167.0 Len: 108 Query Start:467 Query End:574 Subject Strand: POSITIVE Subject Start: 549 Subject End: 656 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgagttgttgatttgttgacatgttgatttgttggctgattactgaatactgaccgctaacgattatatcgggcttgcagccctttcgggtaatggttatgcgtgtta............................................................................... ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgagttgttgattcgttgacatgttgatttgttggctgaccgctgaatactgaccgctaacgattatatcaggctttcagccctttcgggtaatggttatgcgtgtta............................................................................... HSP 2 e-value: 3.0E-29 bit: 131.0 Len: 90 Query Start:396 Query End:485 Subject Strand: POSITIVE Subject Start: 446 Subject End: 535 ...........................................................................................................................................................................................................................................................................................................................................................................................................gattccgcaatagaaccaataagaatgaatttaattcaggccttcagcccttataaacctcgcagaggcggtgagttgttgatttgttga........................................................................................................................................................................ ...........................................................................................................................................................................................................................................................................................................................................................................................................gattcggcaatagaaccaataagaatgaatttaattcaggccttcagctcttataaaccccgcagaggcggtgaattgttaaattgttga........................................................................................................................................................................ Subject: tfor_TF1483_TF1484|glycosyltransferase family protein:penicillin binding protein|POSITIVE:POSITIVE|[1578886,1579698]|813 HSP 1 e-value: 4.0E-32 bit: 141.0 Len: 75 Query Start:538 Query End:612 Subject Strand: NEGATIVE Subject Start: 515 Subject End: 589 .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ggcttgcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccatt......................................... .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ggctttcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccatt......................................... HSP 2 e-value: 1.0E-13 bit: 79.8 Len: 52 Query Start:396 Query End:447 Subject Strand: NEGATIVE Subject Start: 576 Subject End: 627 ...........................................................................................................................................................................................................................................................................................................................................................................................................gattccgcaatagaaccaataagaatgaatttaattcaggccttcagccctt.............................................................................................................................................................................................................. ...........................................................................................................................................................................................................................................................................................................................................................................................................gattccgcaatagaagaaataagaatgaatttaattcaggctttcagccctt.............................................................................................................................................................................................................. HSP 3 e-value: 2.0E-9 bit: 65.9 Len: 45 Query Start:585 Query End:629 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 45 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................catgtacccggcatgacaattaaccattgacaattcaccattaat........................ ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................catgtacccggcataacaattaacaattgacaattaaccattaat........................ HSP 4 e-value: 2.0E-9 bit: 65.9 Len: 45 Query Start:585 Query End:629 Subject Strand: NEGATIVE Subject Start: 299 Subject End: 343 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................catgtacccggcatgacaattaaccattgacaattcaccattaat........................ ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................catgtacccggcataacaattaacaattgacaattaaccattaat........................ Subject: tfor_TF1473_TF1474|hypothetical protein:6- phosphogluconolactonase|POSITIVE:POSITIVE|[1567027, 1567481]|455 HSP 1 e-value: 1.0E-31 bit: 139.0 Len: 78 Query Start:535 Query End:612 Subject Strand: NEGATIVE Subject Start: 195 Subject End: 272 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tcgggcttgcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccatt......................................... ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tcgggctttcagccctttcgggtaatggttatgcgtgttacacgctgaatcatgtacccggcatgacaattaaccatt......................................... HSP 2 e-value: 3.0E-17 bit: 91.7 Len: 58 Query Start:390 Query End:447 Subject Strand: NEGATIVE Subject Start: 327 Subject End: 384 .....................................................................................................................................................................................................................................................................................................................................................................................................aacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagccctt.............................................................................................................................................................................................................. .....................................................................................................................................................................................................................................................................................................................................................................................................aacccagattcggcaatagaacaaataaggatgaatttaattcaggccttcagccctt.............................................................................................................................................................................................................. Subject: tfor_TF0585_TF0586|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[616824,617452]|629 HSP 1 e-value: 1.0E-31 bit: 139.0 Len: 82 Query Start:531 Query End:612 Subject Strand: POSITIVE Subject Start: 286 Subject End: 367 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tatatcgggcttgcagccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccatt......................................... ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tatatctggctttcagccctttcgggtaatggttatgtgtgttacacgccgaatcatgtacccggcatgacaattaaccatt......................................... HSP 2 e-value: 8.0E-24 bit: 113.0 Len: 57 Query Start:390 Query End:446 Subject Strand: POSITIVE Subject Start: 178 Subject End: 234 .....................................................................................................................................................................................................................................................................................................................................................................................................aacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagccct............................................................................................................................................................................................................... .....................................................................................................................................................................................................................................................................................................................................................................................................aacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagccct............................................................................................................................................................................................................... Subject: tfor_TF0021_TF0022|outer membrane phospholipase A:two-component system sensor histidine kinase|POSITIVE:POSITIVE|[16980,17434]|455 HSP 1 e-value: 5.0E-22 bit: 107.0 Len: 74 Query Start:546 Query End:619 Subject Strand: NEGATIVE Subject Start: 52 Subject End: 125 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gccctttcgggtaatggttatgcgtgttacacgccgaatcatgtacccggcatgacaattaaccattgacaatt.................................. .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gccctttcgggtaatgggtatgcgcattacacgtcgaatcatgtacccggcatgacaattcaccattgacaatt.................................. HSP 2 e-value: 1.0E-10 bit: 69.9 Len: 39 Query Start:408 Query End:446 Subject Strand: NEGATIVE Subject Start: 192 Subject End: 230 .......................................................................................................................................................................................................................................................................................................................................................................................................................gaaccaataagaatgaatttaattcaggccttcagccct............................................................................................................................................................................................................... .......................................................................................................................................................................................................................................................................................................................................................................................................................gaacaaataagaatgaatttaattcaggccttcagccct............................................................................................................................................................................................................... Subject: tfor_TF2047_TF2048|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2210359,2210782]|424 HSP 1 e-value: 1.0E-16 bit: 89.7 Len: 57 Query Start:390 Query End:446 Subject Strand: NEGATIVE Subject Start: 337 Subject End: 393 .....................................................................................................................................................................................................................................................................................................................................................................................................aacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagccct............................................................................................................................................................................................................... .....................................................................................................................................................................................................................................................................................................................................................................................................aacccagattccgcaatagaagaaataagaatgaatttaattcaggctttcagccct............................................................................................................................................................................................................... HSP 2 e-value: 3.0E-14 bit: 81.8 Len: 73 Query Start:427 Query End:499 Subject Strand: NEGATIVE Subject Start: 211 Subject End: 283 ..........................................................................................................................................................................................................................................................................................................................................................................................................................................taattcaggccttcagcccttataaacctcgcagaggcggtgagttgttgatttgttgacatgttgatttgtt.......................................................................................................................................................... ..........................................................................................................................................................................................................................................................................................................................................................................................................................................taattcgggctttcagccctcgtaaaccccgcagaggcggtgagttgttgaattgttgagatgtttatttgtt.......................................................................................................................................................... Subject: tfor_TF0677_TF0678|ATP-binding protein, Mrp/Nbp35 family, involved in chromosome partitioning:hypothetical protein|POSITIVE:POSITIVE|[721043,721242]|200 HSP 1 e-value: 1.0E-16 bit: 89.7 Len: 57 Query Start:390 Query End:446 Subject Strand: POSITIVE Subject Start: 8 Subject End: 64 .....................................................................................................................................................................................................................................................................................................................................................................................................aacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagccct............................................................................................................................................................................................................... .....................................................................................................................................................................................................................................................................................................................................................................................................aacccagattacgcaatagaagaaataagaatgaatttaattcaggccttcagccct............................................................................................................................................................................................................... HSP 2 e-value: 7.0E-12 bit: 73.8 Len: 41 Query Start:557 Query End:597 Subject Strand: POSITIVE Subject Start: 149 Subject End: 189 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................taatggttatgcgtgttacacgccgaatcatgtacccggca........................................................ ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................taatggttatgcgtggtacacgccgaatcatgtacccggca........................................................ Subject: tfor_TF2190_TF2191|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2365707,2366073]|367 HSP 1 e-value: 3.0E-14 bit: 81.8 Len: 57 Query Start:390 Query End:446 Subject Strand: POSITIVE Subject Start: 130 Subject End: 186 .....................................................................................................................................................................................................................................................................................................................................................................................................aacccagattccgcaatagaaccaataagaatgaatttaattcaggccttcagccct............................................................................................................................................................................................................... .....................................................................................................................................................................................................................................................................................................................................................................................................aacccggattacgcaatagaagaaataagaatgaatttaattcaggccttcagccct............................................................................................................................................................................................................... HSP 2 e-value: 4.0E-7 bit: 58.0 Len: 41 Query Start:557 Query End:597 Subject Strand: POSITIVE Subject Start: 271 Subject End: 311 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................taatggttatgcgtgttacacgccgaatcatgtacccggca........................................................ ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................taatggttatgcgcggtacactccgaatcatgtacccggca........................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCCUUUCUUAUCACGCUUUGCAGCGGGCUCCCCUCUCGAAAGCGGGUGCAAAAGUACGGAAUAUCUUCUUUCCCUACAAAUAUUUUAUGACCUUUUUUUGAAGAAAAAGCGCAAAUUGUUAAGAAACAACUAUUUAUAAGUAAACAAAAACUUUACAGCGCUACAUUCCUCUGGAUAUAAAAUAAUGUAUCGCGCACAUUAGAUAACAAAGCAGAUAUUCUCUCUUACCCGACUUAUAAGAGCGAACUUUAUCGUAAUAUUCGAUCUGCCUUUCAACCCAGAUUCCGCAAUAGAACCAAUAAGAAUGAAUUUAAUUCAGGCCUUCAGCCCUUAUAAACCUCGCAGAGGCGGUGAGUUGUUGAUUUGUUGACAUGUUGAUUUGUUGGCUGAUUACUGAAUACUGACCGCUAACGAUUAUAUCGGGCUUGCAGCCCUUUCGGGUAAUGGUUAUGCGUGUUACACGCCGAAUCAUGUACCCGGCAUGACAAUUAACCAUUGACAAUUCACCAUUAAUAAAUAGGAAUACAUACCGUUAUUA ....(((((((....((((((.((((..((...........(((((((((...(((.((((........)))).)))...(((((((((.((.......((.(((..(((((...(((((....)))))..........(((((.......))))).)))))...))).)).)).))))))))).)))))).)))...............(((((..(((.((((((.........)))))).))).....(((.......))))))))...........))..))))..........(((((..(((((...)))))(((.....)))))))).......))))))..((((((((((..((..((((.(((((....((((((((.(.(((........)))).)))))))).......((((.....))))...((((((((((((..(((((...)))))..)))))).))))))))))).)))).....))..))))))))))........)))))))............... (-135.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAACGGUAUGUAUUCCUAUUUAUUAAUGGUGAAUUGUCAAUGGUUAAUUGUCAUGCCGGGUACAUGAUUCGGCGUGUAACACGCAUAACCAUUACCCGAAAGGGCUGCAAGCCCGAUAUAAUCGUUAGCGGUCAGUAUUCAGUAAUCAGCCAACAAAUCAACAUGUCAACAAAUCAACAACUCACCGCCUCUGCGAGGUUUAUAAGGGCUGAAGGCCUGAAUUAAAUUCAUUCUUAUUGGUUCUAUUGCGGAAUCUGGGUUGAAAGGCAGAUCGAAUAUUACGAUAAAGUUCGCUCUUAUAAGUCGGGUAAGAGAGAAUAUCUGCUUUGUUAUCUAAUGUGCGCGAUACAUUAUUUUAUAUCCAGAGGAAUGUAGCGCUGUAAAGUUUUUGUUUACUUAUAAAUAGUUGUUUCUUAACAAUUUGCGCUUUUUCUUCAAAAAAAGGUCAUAAAAUAUUUGUAGGGAAAGAAGAUAUUCCGUACUUUUGCACCCGCUUUCGAGAGGGGAGCCCGCUGCAAAGCGUGAUAAGAAAGGA .....((((.((((.(((............(((((.((((...(((((..(((.((((.((((((.(((.....(((((...))))).....)))))))))....(((((...((..(((((.((((....))))..)))))..))...)))))..........)))).)))..))))))))).)))))..(((((((((.(((((.(((((..((((..(((((...)))))..))).)..)))))((((((.(...(((((...(((((((((((.......)))....((((.(((((((.......))))))).)))).))))))))...)))))...).)))))).................((((((...(((((....((((........))))......(((((((....))))))).)))))..))))))...........)))))...)))))))))...))).)))).)))).(((((((.(((.(((....)))))).)).((((....))))......))))).. (-145.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1024 480 431 156 107 tfor:TF1516|5end_transposase_fragment_1626407..1626637_POSITIVE 862 480 431 163 107 tfor:TF2202|5end_hypothetical_protein_2378682..2378912_POSITIVE 707 330 281 160 111 tfor:TF3082|5end_conserved_hypothetical_protein_3316431..3316661_POSITIVE 693 500 451 180 124 tfor:TF2994|5end_hypothetical_protein_3210605..3210835_POSITIVE 691 500 451 195 151 tfor:TF0469|5end_hypothetical_protein_486960..487190_POSITIVE 691 500 451 164 120 tfor:TF0468|5end_hypothetical_protein_486929..487159_POSITIVE 681 330 281 178 129 tfor:TF2048|5end_conserved_hypothetical_protein_2210643..2210873_POSITIVE 681 330 281 153 104 tfor:TF1275|5end_transcription-repair_coupling_factor_1357866..1358096_POSITIVE 662 330 281 218 169 tfor:TF1474|5end_6-phosphogluconolactonase_1567342..1567572_POSITIVE 620 447 401 231 187 tfor:TF0341|5end_conserved_hypothetical_protein_364348..364578_POSITIVE 580 439 394 215 173 tfor:TF0367|5end_eukaryotic-like__metalloproteinase_384007..384237_POSITIVE 580 439 394 215 173 tfor:TF0364|5end_ysyl_endopeptidase_380395..380625_POSITIVE 553 500 451 109 51 tfor:TF2666|5end_hypothetical_protein_2841818..2842048_POSITIVE 551 504 462 107 66 tfor:TF1842|5end_hypothetical_protein_1992571..1992801_POSITIVE 544 500 451 231 188 tfor:TF2818|5end_acetylornithine_aminotransferase_3007739..3007969_POSITIVE 542 500 451 225 182 tfor:TF1850|5end_Na+/H+-exchanging_protein_1998542..1998772_POSITIVE 534 330 281 223 181 tfor:TF2204|5end_conserved_hypothetical_protein_2378866..2379096_POSITIVE 525 500 451 200 142 tfor:TF0470|5end_hypothetical_protein_487253..487483_POSITIVE 520 399 352 53 1 tfor:TF1274|5end_hypothetical_protein_1357621..1357851_POSITIVE 499 504 462 99 58 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 829 479 431 98 43 tfor:TF3003|3end_hypothetical_protein_3220629..3220779_POSITIVE 687 468 423 114 63 tfor:TF2172|3end_hypothetical_protein_2346135..2346285_POSITIVE 681 330 281 102 53 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 636 448 401 61 16 tfor:TF0340|3end_hypothetical_protein_364257..364407_POSITIVE 622 508 466 43 1 tfor:TF0021|3end_outer_membrane_phospholipase_A_16919..17069_POSITIVE 616 514 472 52 10 tfor:TF2817|3end_hypothetical_protein_3007626..3007776_POSITIVE 577 510 462 86 39 tfor:TF1483|3end_glycosyltransferase_family_protein_1578825..1578975_POSITIVE 548 500 451 121 70 tfor:TF1578|3end_hypothetical_protein_1686416..1686566_POSITIVE 541 499 451 64 14 tfor:TF2025|3end_hypothetical_protein_2182180..2182330_POSITIVE 525 500 451 95 37 tfor:TF0469|3end_hypothetical_protein_487228..487378_POSITIVE 469 479 435 149 105 tfor:TF2840|3end_hypothetical_protein_3031289..3031439_POSITIVE 458 500 451 55 11 tfor:TF2921|3end_hypothetical_protein_3118790..3118940_POSITIVE 444 475 440 129 93 tfor:TF2997|3end_hypothetical_protein_3211664..3211814_POSITIVE 433 475 433 46 1 tfor:TF2173|3end_hypothetical_protein_2346063..2346213_POSITIVE 433 475 433 46 1 tfor:TF2166|3end_hypothetical_protein_2342362..2342512_POSITIVE 433 475 433 46 1 tfor:TF0366|3end_hypothetical_protein_383811..383961_POSITIVE 433 475 433 46 1 tfor:TF0346|3end_hypothetical_protein_367316..367466_POSITIVE 427 479 432 118 67 tfor:TF0027|3end_conserved_hypothetical_protein_24199..24349_POSITIVE 419 500 455 55 1 tfor:TF2438|3end_hypothetical_protein_2616157..2616307_POSITIVE 414 500 455 55 1 tfor:TF0468|3end_hypothetical_protein_487188..487338_POSITIVE