Origin IGS: tgaaaaagttatacttcgcgaggcacttgtaaaagcacttaaataaaatcttgcgccaaaaaaacatcttgattgcgtgttgattcctctaatttagataagaaaaagtctgtttcttttattttgtcttctactatcccaaaacttctcattacacttca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaagtgtaatgagaagttttgggatagtagaagacaaaataaaagaaacagactttttcttatctaaattagaggaatcaacacgcaatcaagatgtttttttggcgcaagattttatttaagtgcttttacaagtgcctcgcgaagtataactttttca Mask Tandem Repeat Region ================================================ tgaaaaagttatacttcgcgaggcacttgtaaaagcacttaaataaaatcttgcgccaaaaaaacatcttgattgcgtgttgattcctctaatttagataagaaaaagtctgtttcttttattttgtcttctactatcccaaaacttctcattacacttca Find is-nt database================================================ Query_seq: TF1641:TF1642|TF1641:TF1642:hypothetical protein:conserved hypothetical protein:->->:1749247..1749407 161 tgaaaaagttatacttcgcgaggcacttgtaaaagcacttaaataaaatcttgcgccaaaaaaacatcttgattgcgtgttgattcctctaatttagataagaaaaagtctgtttcttttattttgtcttctactatcccaaaacttctcattacacttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1641:TF1642|TF1641:TF1642:hypothetical protein:conserved hypothetical protein:->->:1749247..1749407 161 tgaaaaagttatacttcgcgaggcacttgtaaaagcacttaaataaaatcttgcgccaaaaaaacatcttgattgcgtgttgattcctctaatttagataagaaaaagtctgtttcttttattttgtcttctactatcccaaaacttctcattacacttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1641:TF1642|TF1641:TF1642:hypothetical protein:conserved hypothetical protein:->->:1749247..1749407 161 tgaaaaagttatacttcgcgaggcacttgtaaaagcacttaaataaaatcttgcgccaaaaaaacatcttgattgcgtgttgattcctctaatttagataagaaaaagtctgtttcttttattttgtcttctactatcccaaaacttctcattacacttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgaaaaagttatacttcgcgaggcacttgtaaaagcacttaaataaaatcttgcgccaaaaaaacatcttgattgcgtgttgattcctctaatttagataagaaaaagtctgtttcttttattttgtcttctactatcccaaaacttctcattacacttca Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: - Start: 42 End: 152 ......................................... I F D Q A L F V D Q N R T S E E L K S L F F D T E K I K D E V I G F S R M ......... Protein_Len: 31 Strand: - Start: 1 End: 93 S F T I S R S A S T F A S L Y F R A G F F C R S Q T N I G R M .................................................................... tgaaaaagttatacttcgcgaggcacttgtaaaagcacttaaataaaatcttgcgccaaaaaaacatcttgattgcgtgttgattcctctaatttagataagaaaaagtctgtttcttttattttgtcttctactatcccaaaacttctcattacacttca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgaaaaagttatacttcgcgaggcacttgtaaaagcacttaaataaaatcttgcgccaaaaaaacatcttgattgcgtgttgattcctctaatttagataagaaaaagtctgtttcttttattttgtcttctactatcccaaaacttctcattacacttca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1641:TF1642|TF1641:TF1642:hypothetical protein:conserved hypothetical protein:->->:1749247..1749407 161 tgaaaaagttatacttcgcgaggcacttgtaaaagcacttaaataaaatcttgcgccaaaaaaacatcttgattgcgtgttgattcctctaatttagataagaaaaagtctgtttcttttattttgtcttctactatcccaaaacttctcattacacttca Intra-Species Hit: Count: 1 Min: 1 Max: 161 Len: 161 Subject: tfor_TF1641_TF1642|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1749247,1749407]|161 HSP 1 e-value: 5.0E-74 bit: 278.0 Len: 161 Query Start:1 Query End:161 Subject Strand: POSITIVE Subject Start: 1 Subject End: 161 tgaaaaagttatacttcgcgaggcacttgtaaaagcacttaaataaaatcttgcgccnnnnnnncatcttgattgcgtgttgattcctctaatttagataagaaaaagtctgtttcttttattttgtcttctactatcccaaaacttctcattacacttca tgaaaaagttatacttcgcgaggcacttgtaaaagcacttaaataaaatcttgcgccaaaaaaacatcttgattgcgtgttgattcctctaatttagataagaaaaagtctgtttcttttattttgtcttctactatcccaaaacttctcattacacttca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAAAAGUUAUACUUCGCGAGGCACUUGUAAAAGCACUUAAAUAAAAUCUUGCGCCAAAAAAACAUCUUGAUUGCGUGUUGAUUCCUCUAAUUUAGAUAAGAAAAAGUCUGUUUCUUUUAUUUUGUCUUCUACUAUCCCAAAACUUCUCAUUACACUUCA (((..(((((.........((((..(((....)))...........((((..(((((((..((.....))..))).)))).))))))))......(((((((((((((.......))))).)))))))).............))))).))).......... (-19.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAGUGUAAUGAGAAGUUUUGGGAUAGUAGAAGACAAAAUAAAAGAAACAGACUUUUUCUUAUCUAAAUUAGAGGAAUCAACACGCAAUCAAGAUGUUUUUUUGGCGCAAGAUUUUAUUUAAGUGCUUUUACAAGUGCCUCGCGAAGUAUAACUUUUUCA .((((.((.(((....((...((.((.(((((((((.((((((((......((.(((((((..........))))))))).....((..((((((.....)))))).))....))))))))..)).)))))))..)).))..))....))).))..)))). (-24.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 292 40 8 59 17 tfor:TF0180|5end_carboxynorspermidine_decarboxylase_191267..191497_POSITIVE 271 57 11 121 62 tfor:TF2039|5end_possible_cell-cycle_regulation_histidine_triad_(HIT)_protein_2199318..2199548_POSITIVE 261 56 12 51 2 tfor:TF2482|5end_hypothetical_protein_2658503..2658733_POSITIVE 259 57 15 110 63 tfor:TF1800|5end_conserved_hypothetical_protein_1941214..1941444_POSITIVE 247 35 13 228 205 tfor:TF0941|5end_transposase_fragment_981973..982203_POSITIVE 240 140 109 147 120 tfor:TF1852|5end_prtQ_protein_2004643..2004873_POSITIVE 238 57 14 109 50 tfor:TF0310|5end_conserved_hypothetical_protein_334685..334915_POSITIVE 237 159 120 69 32 tfor:TF1155|5end_co-chaperonin,_groES_family;__heat_shock_protein_10_1236804..1237034_POSITIVE 236 90 50 61 28 tfor:TF2283|5end_hypothetical_protein_2440266..2440496_POSITIVE 235 57 17 46 5 tfor:TF1462|5end_erythronate-4-phosphate_dehydrogenase_1552184..1552414_POSITIVE 232 40 14 165 144 tfor:TF1639|5end_conserved_hypothetical_protein_1747652..1747882_POSITIVE 232 57 14 61 28 tfor:TF0831|5end_conserved_hypothetical_protein_888183..888413_POSITIVE 232 55 15 164 117 tfor:TF0076|5end_probable_carbohydrate_kinase,_PfkB_family_83279..83509_POSITIVE 228 89 54 215 178 tfor:TF2351|5end_acetate_kinase_2515480..2515710_POSITIVE 227 87 51 221 181 tfor:TF1027|5end_hypothetical_protein_1085641..1085871_POSITIVE 227 59 13 157 101 tfor:TF0409|5end_conserved_hypothetical_protein_426240..426470_POSITIVE 225 39 16 177 154 tfor:TF2159|5end_conserved_hypothetical_protein_2334446..2334676_POSITIVE 223 150 107 118 79 tfor:TF2844|5end_possible_phosphatase_or_hydrolase_3033680..3033910_POSITIVE 223 56 14 214 180 tfor:TF2820|5end_argininosuccinate_synthetase_3009876..3010106_POSITIVE 223 76 34 42 6 tfor:TF0669|5end_hypothetical_protein_713384..713614_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 280 89 49 138 99 tfor:TF1968|3end_conserved_hypothetical_protein_2115959..2116109_POSITIVE 261 56 12 51 2 tfor:TF2481|3end_possible_thiol_protease/hemagglutinin_PrtT_2658583..2658733_POSITIVE 259 57 15 110 63 tfor:TF1799|3end_quinolinate_synthetase_1941294..1941444_POSITIVE 240 140 109 105 78 tfor:TF1851|3end_methionine_synthase_I_2004681..2004831_POSITIVE 240 96 53 75 18 tfor:TF0789|3end_preprotein_translocase,_secDF_family_843670..843820_POSITIVE 238 57 14 119 60 tfor:TF0309|3end_conserved_hypothetical_protein_334775..334925_POSITIVE 232 57 14 48 15 tfor:TF0829|3end_conserved_hypothetical_protein_888250..888400_POSITIVE 228 90 50 70 33 tfor:TF1382|3end_hypothetical_protein_1457777..1457927_POSITIVE 228 90 50 66 29 tfor:TF1383|3end_hypothetical_protein_1457773..1457923_POSITIVE 227 87 51 139 99 tfor:TF1026|3end_beta-N-acetylglucosaminidase;_beta_lactamase_1085639..1085789_POSITIVE 223 150 107 118 79 tfor:TF2843|3end_conserved_hypothetical_protein;_possible_lipoprotein_3033760..3033910_POSITIVE 222 31 9 95 72 tfor:TF1187|3end_hypothetical_protein_1269872..1270022_POSITIVE 222 31 9 38 15 tfor:TF1186|3end_hypothetical_protein_1269815..1269965_POSITIVE 221 110 64 85 32 tfor:TF1430|3end_hypothetical_protein_1515153..1515303_POSITIVE 220 57 11 63 20 tfor:TF2583|3end_possible_ABC_transporter,__permease_component_2751026..2751176_POSITIVE 219 60 11 57 2 tfor:TF2978|3end_conserved_hypothetical_protein_3190862..3191012_POSITIVE 215 93 51 146 98 tfor:TF0556|3end_Bacteroides_aerotolerance_protein,_BatA_589149..589299_POSITIVE 213 89 47 78 44 tfor:TF2640|3end_hypothetical_protein_2809533..2809683_POSITIVE 212 100 53 59 4 tfor:TF2573|3end_50S_ribosomal_protein_L15_2743857..2744007_POSITIVE 210 58 18 70 16 tfor:TF2126|3end_S-adenosylhomocysteine_hydrolase_2303996..2304146_POSITIVE