Origin IGS: tattcattcaggctttcagccctccgctgagggggttgccttgtttcccaacgctttgcattgggctgaattaacacgccctttcagggctgttttccaattgttagttgttaatggttatttgccgggtacatgattcgacgtgtaccacgcataacaattgaccattaaccattatcaccgctcgcacggggcacaagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcgattcccataccccgtttggagggctgaaagcctgatataatcgttggcaatcagtagtttgcccgttatcacacaccgcccagggggagatgtctgtccgagaaatgtgctttccggccgccgctgcggcttcggagtgcaaggtagagacgcccttaaacgggcgtctctacacctcatgctacattca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaatgtagcatgaggtgtagagacgcccgtttaagggcgtctctaccttgcactccgaagccgcagcggcggccggaaagcacatttctcggacagacatctccccctgggcggtgtgtgataacgggcaaactactgattgccaacgattatatcaggctttcagccctccaaacggggtatgggaatcgataccccacccttgcgggatggggctgaaatatatcgggcttgcagcccttgtgccccgtgcgagcggtgataatggttaatggtcaattgttatgcgtggtacacgtcgaatcatgtacccggcaaataaccattaacaactaacaattggaaaacagccctgaaagggcgtgttaattcagcccaatgcaaagcgttgggaaacaaggcaaccccctcagcggagggctgaaagcctgaatgaata Mask Tandem Repeat Region ================================================ tattcattcaggctttcagccctccgctgagggggttgccttgtttcccaacgctttgcattgggctgaattaacacgccctttcagggctgttttccaattgttagttgttaatggttatttgccgggtacatgattcgacgtgtaccacgcataacaattgaccattaaccattatcaccgctcgcacggggcacaagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcgattcccataccccgtttggagggctgaaagcctgatataatcgttggcaatcagtagtttgcccgttatcacacaccgcccagggggagatgtctgtccgagaaatgtgctttccggccgccgctgcggcttcggagtgcaaggtagagacgcccttaaacgggcgtctctacacctcatgctacattca Find is-nt database================================================ Query_seq: TF1585:TF1586|TF1585:TF1586:hypothetical protein:hypothetical protein:->->:1690671..1691110 440 tattcattcaggctttcagccctccgctgagggggttgccttgtttcccaacgctttgcattgggctgaattaacacgccctttcagggctgttttccaattgttagttgttaatggttatttgccgggtacatgattcgacgtgtaccacgcataacaattgaccattaaccattatcaccgctcgcacggggcacaagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcgattcccataccccgtttggagggctgaaagcctgatataatcgttggcaatcagtagtttgcccgttatcacacaccgcccagggggagatgtctgtccgagaaatgtgctttccggccgccgctgcggcttcggagtgcaaggtagagacgcccttaaacgggcgtctctacacctcatgctacattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1585:TF1586|TF1585:TF1586:hypothetical protein:hypothetical protein:->->:1690671..1691110 440 tattcattcaggctttcagccctccgctgagggggttgccttgtttcccaacgctttgcattgggctgaattaacacgccctttcagggctgttttccaattgttagttgttaatggttatttgccgggtacatgattcgacgtgtaccacgcataacaattgaccattaaccattatcaccgctcgcacggggcacaagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcgattcccataccccgtttggagggctgaaagcctgatataatcgttggcaatcagtagtttgcccgttatcacacaccgcccagggggagatgtctgtccgagaaatgtgctttccggccgccgctgcggcttcggagtgcaaggtagagacgcccttaaacgggcgtctctacacctcatgctacattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1585:TF1586|TF1585:TF1586:hypothetical protein:hypothetical protein:->->:1690671..1691110 440 tattcattcaggctttcagccctccgctgagggggttgccttgtttcccaacgctttgcattgggctgaattaacacgccctttcagggctgttttccaattgttagttgttaatggttatttgccgggtacatgattcgacgtgtaccacgcataacaattgaccattaaccattatcaccgctcgcacggggcacaagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcgattcccataccccgtttggagggctgaaagcctgatataatcgttggcaatcagtagtttgcccgttatcacacaccgcccagggggagatgtctgtccgagaaatgtgctttccggccgccgctgcggcttcggagtgcaaggtagagacgcccttaaacgggcgtctctacacctcatgctacattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tattcattcaggctttcagccctccgctgagggggttgccttgtttcccaacgctttgcattgggctgaattaacacgccctttcagggctgttttccaattgttagttgttaatggttatttgccgggtacatgattcgacgtgtaccacgcataacaattgaccattaaccattatcaccgctcgcacggggcacaagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcgattcccataccccgtttggagggctgaaagcctgatataatcgttggcaatcagtagtttgcccgttatcacacaccgcccagggggagatgtctgtccgagaaatgtgctttccggccgccgctgcggcttcggagtgcaaggtagagacgcccttaaacgggcgtctctacacctcatgctacattca Predict ORF larger than 30AA ================================================ Protein_Len: 44 Strand: + Start: 2 End: 133 . M H S G F Q P S A E G V A L F P N A L H W A E L T R P F R A V F Q L L V V N G Y L P G T ................................................................................................................................................................................................................................................................................................................... Protein_Len: 38 Strand: + Start: 161 End: 274 ................................................................................................................................................................ M T I N H Y H R S H G A Q G L Q A R Y I S A P S R K G G V S I P I P R L E G ...................................................................................................................................................................... Protein_Len: 38 Strand: + Start: 174 End: 287 ............................................................................................................................................................................. M I T A R T G H K G C K P D I F Q P H P A R V G Y R F P Y P V W R A E S L I ......................................................................................................................................................... Protein_Len: 33 Strand: + Start: 340 End: 438 ................................................................................................................................................................................................................................................................................................................................................... M S V R E M C F P A A A A A S E C K V E T P L N G R L Y T S C Y I .. Protein_Len: 49 Strand: + Start: 294 End: 440 ..................................................................................................................................................................................................................................................................................................... M A I S S L P V I T H R P G G D V C P R N V L S G R R C G F G V Q G R D A L K R A S L H L M L H S Protein_Len: 60 Strand: - Start: 224 End: 430 ............................................................................................................................................................................................................................... N G Y G T Q L A S L R I Y D N A I L L K G T I V C R G P P S T Q G L F T S E P R R Q P K P T C P L S A R L R A D R C R M .......... Protein_Len: 40 Strand: - Start: 181 End: 300 .................................................................................................................................................................................... R E C P A C P S C A R Y I E A G D R L P P T D I G M G R K S P Q F G S I I T P M ............................................................................................................................................ Protein_Len: 60 Strand: - Start: 171 End: 392 .......................................................................................................................................................................... I Y K L G M G C P H P I S E W V G N P P S F A Q Y L R Q C D T T Q G N D C V A W P S I D T R S I H K G A A A A A E S H M ................................................ Protein_Len: 37 Strand: - Start: 107 End: 217 .......................................................................................................... N N I T I Q R T C S E V H V V C L L Q G N V M I V A R V P C L P Q L G S M ............................................................................................................................................................................................................................... tattcattcaggctttcagccctccgctgagggggttgccttgtttcccaacgctttgcattgggctgaattaacacgccctttcagggctgttttccaattgttagttgttaatggttatttgccgggtacatgattcgacgtgtaccacgcataacaattgaccattaaccattatcaccgctcgcacggggcacaagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcgattcccataccccgtttggagggctgaaagcctgatataatcgttggcaatcagtagtttgcccgttatcacacaccgcccagggggagatgtctgtccgagaaatgtgctttccggccgccgctgcggcttcggagtgcaaggtagagacgcccttaaacgggcgtctctacacctcatgctacattca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tattcattcaggctttcagccctccgctgagggggttgccttgtttcccaacgctttgcattgggctgaattaacacgccctttcagggctgttttccaattgttagttgttaatggttatttgccgggtacatgattcgacgtgtaccacgcataacaattgaccattaaccattatcaccgctcgcacggggcacaagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcgattcccataccccgtttggagggctgaaagcctgatataatcgttggcaatcagtagtttgcccgttatcacacaccgcccagggggagatgtctgtccgagaaatgtgctttccggccgccgctgcggcttcggagtgcaaggtagagacgcccttaaacgggcgtctctacacctcatgctacattca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 70 HP_score: -6.3 Tail_Score: -4.24123 Start: 75 End: 92 Full_Region: ATTGGGCTGAATTAA CACGCCCT TTC AGGGCTG TTTTCCAATTGTTAG ..........................................................................CACGCCCTTTCAGGGCTG............................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 76 HP_score: -9.1 Tail_Score: -3.71291 Start: 78 End: 90 Full_Region: GGGCTGAATTAACAC GCCCT TTC AGGGC TGTTTTCCAATTGTT .............................................................................GCCCTTTCAGGGC.............................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 47 HP_score: -5.6 Tail_Score: -2.53876 Start: 223 End: 247 Full_Region: AGCCCGATATATTTC AGCCCCATCC CGCAA GGGTGGGGTA TCGATTCCCATACCC ..............................................................................................................................................................................................................................AGCCCCATCCCGCAAGGGTGGGGTA................................................................................................................................................................................................. Find igs database================================================ Query_seq: TF1585:TF1586|TF1585:TF1586:hypothetical protein:hypothetical protein:->->:1690671..1691110 440 tattcattcaggctttcagccctccgctgagggggttgccttgtttcccaacgctttgcattgggctgaattaacacgccctttcagggctgttttccaattgttagttgttaatggttatttgccgggtacatgattcgacgtgtaccacgcataacaattgaccattaaccattatcaccgctcgcacggggcacaagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcgattcccataccccgtttggagggctgaaagcctgatataatcgttggcaatcagtagtttgcccgttatcacacaccgcccagggggagatgtctgtccgagaaatgtgctttccggccgccgctgcggcttcggagtgcaaggtagagacgcccttaaacgggcgtctctacacctcatgctacattca Intra-Species Hit: Count: 19 Min: 1 Max: 440 Len: 440 Subject: tfor_TF1585_TF1586|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1690671,1691110]|440 HSP 1 e-value: 0.0 bit: 872.0 Len: 440 Query Start:1 Query End:440 Subject Strand: POSITIVE Subject Start: 1 Subject End: 440 tattcattcaggctttcagccctccgctgagggggttgccttgtttcccaacgctttgcattgggctgaattaacacgccctttcagggctgttttccaattgttagttgttaatggttatttgccgggtacatgattcgacgtgtaccacgcataacaattgaccattaaccattatcaccgctcgcacggggcacaagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcgattcccataccccgtttggagggctgaaagcctgatataatcgttggcaatcagtagtttgcccgttatcacacaccgcccagggggagatgtctgtccgagaaatgtgctttccggccgccgctgcggcttcggagtgcaaggtagagacgcccttaaacgggcgtctctacacctcatgctacattca tattcattcaggctttcagccctccgctgagggggttgccttgtttcccaacgctttgcattgggctgaattaacacgccctttcagggctgttttccaattgttagttgttaatggttatttgccgggtacatgattcgacgtgtaccacgcataacaattgaccattaaccattatcaccgctcgcacggggcacaagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcgattcccataccccgtttggagggctgaaagcctgatataatcgttggcaatcagtagtttgcccgttatcacacaccgcccagggggagatgtctgtccgagaaatgtgctttccggccgccgctgcggcttcggagtgcaaggtagagacgcccttaaacgggcgtctctacacctcatgctacattca Subject: tfor_TF0467_TF0468|acetyltransferase-related protein:hypothetical protein|POSITIVE:POSITIVE|[486604,487068]|465 HSP 1 e-value: 3.0E-38 bit: 161.0 Len: 105 Query Start:58 Query End:162 Subject Strand: POSITIVE Subject Start: 341 Subject End: 445 .........................................................gcattgggctgaattaacacgccctttcagggctgttttccaattgttagttgttaatggttatttgccgggtacatgattcgacgtgtaccacgcataacaatt...................................................................................................................................................................................................................................................................................... .........................................................gcattgggctgaattattacgccctttcagggctgttttccaattgttaattgtcaatggttatttgctgggtacatgattcggcgtgtaccacgcataacaatt...................................................................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-18 bit: 95.6 Len: 56 Query Start:1 Query End:56 Subject Strand: POSITIVE Subject Start: 274 Subject End: 329 tattcattcaggctttcagccctccgctgagggggttgccttgtttcccaacgctt................................................................................................................................................................................................................................................................................................................................................................................................ tattcattcaggctttcagccctccgccgagggggttgccctgtttcccaacgctt................................................................................................................................................................................................................................................................................................................................................................................................ Subject: tfor_TF2815_TF2816|meso-diaminopimelate D-dehydrogenase:hypothetical protein|POSITIVE:POSITIVE|[3006481,3007064]|584 HSP 1 e-value: 2.0E-29 bit: 131.0 Len: 106 Query Start:186 Query End:291 Subject Strand: POSITIVE Subject Start: 381 Subject End: 486 .........................................................................................................................................................................................cgcacggggcacaagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcgattcccataccccgtttggagggctgaaagcctgatataat..................................................................................................................................................... .........................................................................................................................................................................................cgcacagggcacaagggctgaaagcccgatatatttcagccccatcccgtaagggtgggatatcgattcccagaccttgttcagagggctgaaagcctgacataat..................................................................................................................................................... Subject: tfor_TF2920_TF2921|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[3117231,3118637]|1407 HSP 1 e-value: 9.0E-29 bit: 129.0 Len: 109 Query Start:183 Query End:291 Subject Strand: POSITIVE Subject Start: 1010 Subject End: 1118 ......................................................................................................................................................................................gctcgcacggggcacaagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcgattcccataccccgtttggagggctgaaagcctgatataat..................................................................................................................................................... ......................................................................................................................................................................................gctcgcccgaggcccaagggctgaaagcccggtatatttcagccccatcccgtaaggatggggtatcgattccccgaccccgttgggagggctgaaagcctgacataat..................................................................................................................................................... HSP 2 e-value: 3.0E-16 bit: 87.7 Len: 56 Query Start:1 Query End:56 Subject Strand: POSITIVE Subject Start: 504 Subject End: 559 tattcattcaggctttcagccctccgctgagggggttgccttgtttcccaacgctt................................................................................................................................................................................................................................................................................................................................................................................................ tattcattcaggctttcagccctccgccgagggggtcgccctgtttcccaacgctt................................................................................................................................................................................................................................................................................................................................................................................................ HSP 3 e-value: 5.0E-15 bit: 83.8 Len: 46 Query Start:1 Query End:46 Subject Strand: POSITIVE Subject Start: 823 Subject End: 868 tattcattcaggctttcagccctccgctgagggggttgccttgttt.......................................................................................................................................................................................................................................................................................................................................................................................................... tattcattcaggctttcagccctccgctgagggggttgccctgttt.......................................................................................................................................................................................................................................................................................................................................................................................................... HSP 4 e-value: 7.0E-8 bit: 60.0 Len: 34 Query Start:58 Query End:91 Subject Strand: POSITIVE Subject Start: 894 Subject End: 927 .........................................................gcattgggctgaattaacacgccctttcagggct............................................................................................................................................................................................................................................................................................................................................................. .........................................................gcattgggctgaattaatacgccctttcagggct............................................................................................................................................................................................................................................................................................................................................................. HSP 5 e-value: 1.0E-6 bit: 56.0 Len: 36 Query Start:59 Query End:94 Subject Strand: POSITIVE Subject Start: 572 Subject End: 607 ..........................................................cattgggctgaattaacacgccctttcagggctgtt.......................................................................................................................................................................................................................................................................................................................................................... ..........................................................cattggactgaattaacgcgccctttcagggctgtt.......................................................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF2190_TF2191|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2365707,2366073]|367 HSP 1 e-value: 1.0E-27 bit: 125.0 Len: 103 Query Start:67 Query End:169 Subject Strand: NEGATIVE Subject Start: 265 Subject End: 367 ..................................................................tgaattaacacgccctttcagggctgttttccaattgttagttgttaatggttatttgccgggtacatgattcgacgtgtaccacgcataacaattgaccatt............................................................................................................................................................................................................................................................................... ..................................................................tgaattatctcgccctttcagggctgtttttcaattgttaattgtcaatggttatttgccgggtacatgattcggagtgtaccgcgcataaccattaaccatt............................................................................................................................................................................................................................................................................... HSP 2 e-value: 5.0E-15 bit: 83.8 Len: 54 Query Start:198 Query End:251 Subject Strand: NEGATIVE Subject Start: 205 Subject End: 258 .....................................................................................................................................................................................................aagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcga............................................................................................................................................................................................. .....................................................................................................................................................................................................aagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatcga............................................................................................................................................................................................. Subject: tfor_TF2201_TF2202|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2378345,2378821]|477 HSP 1 e-value: 1.0E-24 bit: 115.0 Len: 123 Query Start:47 Query End:169 Subject Strand: POSITIVE Subject Start: 324 Subject End: 450 ..............................................cccaacgctttgcattgggctgaattaacacgccctttcagggctgttttccaattgttagttgttaatggtta----tttgccgggtacatgattcgacgtgtaccacgcataacaattgaccatt............................................................................................................................................................................................................................................................................... ..............................................cccaacgcttcgcattgggctgaattatctcgccctttcagggctgtttattaatggttaattgtcaatggttaattgtctgccgggtacatgattcggcgtgtaccacgcataaccattaaccatt............................................................................................................................................................................................................................................................................... Subject: tfor_TF0821_TF0822|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[880256,880626]|371 HSP 1 e-value: 5.0E-21 bit: 103.0 Len: 60 Query Start:110 Query End:169 Subject Strand: NEGATIVE Subject Start: 300 Subject End: 359 .............................................................................................................gttaatggttatttgccgggtacatgattcgacgtgtaccacgcataacaattgaccatt............................................................................................................................................................................................................................................................................... .............................................................................................................gttaatggttatttgccgggtacatgattcggcgtgtaccacgcataaccattgaccatt............................................................................................................................................................................................................................................................................... HSP 2 e-value: 5.0E-15 bit: 83.8 Len: 54 Query Start:198 Query End:251 Subject Strand: NEGATIVE Subject Start: 240 Subject End: 293 .....................................................................................................................................................................................................aagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcga............................................................................................................................................................................................. .....................................................................................................................................................................................................aagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatcga............................................................................................................................................................................................. Subject: tfor_TF0029_TF0030|hypothetical protein:N-acetylneuraminate lyase|POSITIVE:POSITIVE|[25123,25909]|787 HSP 1 e-value: 5.0E-21 bit: 103.0 Len: 100 Query Start:196 Query End:295 Subject Strand: NEGATIVE Subject Start: 163 Subject End: 262 ...................................................................................................................................................................................................acaagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcgattcccataccccgtttggagggctgaaagcctgatataatcgtt................................................................................................................................................. ...................................................................................................................................................................................................acaagggctggaagcccgaattatttcagccccatcccgtaagggtgggggatccatgcccataccttggttagagggctgtaagcctgatataatcgtt................................................................................................................................................. HSP 2 e-value: 3.0E-7 bit: 58.0 Len: 45 Query Start:47 Query End:91 Subject Strand: NEGATIVE Subject Start: 386 Subject End: 430 ..............................................cccaacgctttgcattgggctgaattaacacgccctttcagggct............................................................................................................................................................................................................................................................................................................................................................. ..............................................cccaacgcttcgcattgggctgaattattatgccctttcagggct............................................................................................................................................................................................................................................................................................................................................................. Subject: tfor_TF1762_TF1763|hypothetical protein:K+-dependent Na+/Ca+ exchanger related-protein|POSITIVE:POSITIVE|[1899709,1900545]|837 HSP 1 e-value: 8.0E-20 bit: 99.6 Len: 70 Query Start:183 Query End:252 Subject Strand: POSITIVE Subject Start: 216 Subject End: 285 ......................................................................................................................................................................................gctcgcacggggcacaagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcgat............................................................................................................................................................................................ ......................................................................................................................................................................................gctcgcccgaggcccaagggctgaaagcccgacatatttcagccccatcccgcaagggtggggtatcgat............................................................................................................................................................................................ HSP 2 e-value: 3.0E-7 bit: 58.0 Len: 33 Query Start:59 Query End:91 Subject Strand: POSITIVE Subject Start: 76 Subject End: 108 ..........................................................cattgggctgaattaacacgccctttcagggct............................................................................................................................................................................................................................................................................................................................................................. ..........................................................cattgggctgaattaacgcgccctttcagggct............................................................................................................................................................................................................................................................................................................................................................. Subject: tfor_TF1841_TF1842|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1992328,1992710]|383 HSP 1 e-value: 8.0E-17 bit: 89.7 Len: 106 Query Start:1 Query End:106 Subject Strand: NEGATIVE Subject Start: 148 Subject End: 264 tattcattcaggctttcagccctccgctgagggggttgcc-ttgtttcccaacg-----ctttgc-----attgggctgaattaacacgccctttcagggctgttttccaattgtta.............................................................................................................................................................................................................................................................................................................................................. tatttattcaggctttcagccctccgccgagggggtcgcccttttttcccaacgcttcgctttgctgcacattgggctgaattaatacgccctttcagggctgtttttcaattgtta.............................................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 4.0E-9 bit: 63.9 Len: 55 Query Start:110 Query End:164 Subject Strand: POSITIVE Subject Start: 105 Subject End: 157 .............................................................................................................gttaatggttatttgccgggtacatgattcgacgtgtaccacgcataacaattga.................................................................................................................................................................................................................................................................................... .............................................................................................................gttaatggttat--gccggatacatgatttggcgtgtaccacgcataacaattga.................................................................................................................................................................................................................................................................................... Subject: tfor_TF0677_TF0678|ATP-binding protein, Mrp/Nbp35 family, involved in chromosome partitioning:hypothetical protein|POSITIVE:POSITIVE|[721043,721242]|200 HSP 1 e-value: 1.0E-15 bit: 85.7 Len: 55 Query Start:115 Query End:169 Subject Strand: NEGATIVE Subject Start: 143 Subject End: 197 ..................................................................................................................tggttatttgccgggtacatgattcgacgtgtaccacgcataacaattgaccatt............................................................................................................................................................................................................................................................................... ..................................................................................................................tggttatttgccgggtacatgattcggcgtgtaccacgcataaccattaaccatt............................................................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-12 bit: 75.8 Len: 54 Query Start:198 Query End:251 Subject Strand: NEGATIVE Subject Start: 83 Subject End: 136 .....................................................................................................................................................................................................aagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcga............................................................................................................................................................................................. .....................................................................................................................................................................................................aagggctgaaagcccgaattatttcagccctatcccgcaagggtggggtatcga............................................................................................................................................................................................. Subject: tfor_TF2477_TF2478|phosphatase:hypothetical protein|POSITIVE:POSITIVE|[2655026,2655514]|489 HSP 1 e-value: 2.0E-14 bit: 81.8 Len: 84 Query Start:11 Query End:94 Subject Strand: NEGATIVE Subject Start: 359 Subject End: 446 ..........ggctttcagccctccgctgagggggttgccttgtttcccaacgctttgc-----attgggctgaattaacacgccctttcagggctgtt.......................................................................................................................................................................................................................................................................................................................................................... ..........ggctttcagccctccgccgaggagattgcctt-tttcccaacgcttcgctacgcattgggctgaattaatacgccctttcagggctgtt.......................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 7.0E-14 bit: 79.8 Len: 76 Query Start:186 Query End:261 Subject Strand: NEGATIVE Subject Start: 220 Subject End: 295 .........................................................................................................................................................................................cgcacggggcacaagggctgcaagcccgatatatttcagccccatcccgcaagggtggggtatcgattcccatacc................................................................................................................................................................................... .........................................................................................................................................................................................cgcaaggggcacaaaggctgaaagtccgatctatttcagccccatcccataaggttggggtatcgatgcccatacc................................................................................................................................................................................... Subject: tfor_TF2047_TF2048|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2210359,2210782]|424 HSP 1 e-value: 1.0E-12 bit: 75.8 Len: 50 Query Start:199 Query End:248 Subject Strand: POSITIVE Subject Start: 264 Subject End: 313 ......................................................................................................................................................................................................agggctgcaagcccgatatatttcagccccatcccgcaagggtggggtat................................................................................................................................................................................................ ......................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgcaagggtggggtat................................................................................................................................................................................................ HSP 2 e-value: 7.0E-11 bit: 69.9 Len: 55 Query Start:37 Query End:91 Subject Strand: POSITIVE Subject Start: 98 Subject End: 152 ....................................tgccttgtttcccaacgctttgcattgggctgaattaacacgccctttcagggct............................................................................................................................................................................................................................................................................................................................................................. ....................................tgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggct............................................................................................................................................................................................................................................................................................................................................................. Subject: tfor_TF2438_TF2439|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2616218,2616519]|302 HSP 1 e-value: 5.0E-12 bit: 73.8 Len: 49 Query Start:8 Query End:56 Subject Strand: POSITIVE Subject Start: 118 Subject End: 166 .......tcaggctttcagccctccgctgagggggttgccttgtttcccaacgctt................................................................................................................................................................................................................................................................................................................................................................................................ .......tcaggctttcagccctccgctgagggggtcgcctgttttcccaacgctt................................................................................................................................................................................................................................................................................................................................................................................................ HSP 2 e-value: 3.0E-10 bit: 67.9 Len: 74 Query Start:98 Query End:171 Subject Strand: POSITIVE Subject Start: 38 Subject End: 111 .................................................................................................caattgttagttgttaatggttatttgccgggtacatgattcgacgtgtaccacgcataacaattgaccattaa............................................................................................................................................................................................................................................................................. .................................................................................................caatggttaattgttaatggttattttctaaatacatgattcggcgtgtaccacgcataacaattaacaattaa............................................................................................................................................................................................................................................................................. Subject: tfor_TF1483_TF1484|glycosyltransferase family protein:penicillin binding protein|POSITIVE:POSITIVE|[1578886,1579698]|813 HSP 1 e-value: 2.0E-11 bit: 71.9 Len: 56 Query Start:1 Query End:56 Subject Strand: NEGATIVE Subject Start: 152 Subject End: 206 tattcattcaggctttcagccctccgctgagggggttgccttgtttcccaacgctt................................................................................................................................................................................................................................................................................................................................................................................................ tattcattcaggctttcagccctccgctga-ggggttgcctgttttcccaatgctt................................................................................................................................................................................................................................................................................................................................................................................................ HSP 2 e-value: 1.0E-6 bit: 56.0 Len: 36 Query Start:123 Query End:158 Subject Strand: POSITIVE Subject Start: 530 Subject End: 565 ..........................................................................................................................tgccgggtacatgattcgacgtgtaccacgcataac.......................................................................................................................................................................................................................................................................................... ..........................................................................................................................tgccgggtacatgattcggcgtgtaacacgcataac.......................................................................................................................................................................................................................................................................................... Subject: tfor_TF1848_TF1850|conserved hypothetical protein:Na+/H+-exchanging protein|POSITIVE:POSITIVE|[1998005,1998681]|677 HSP 1 e-value: 7.0E-11 bit: 69.9 Len: 63 Query Start:59 Query End:121 Subject Strand: NEGATIVE Subject Start: 48 Subject End: 110 ..........................................................cattgggctgaattaacacgccctttcagggctgttttccaattgttagttgttaatggttat............................................................................................................................................................................................................................................................................................................................... ..........................................................cattgggctgaattaacgcgccctttcagggctcttctctaatggttaattgtcaatggttat............................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 3.0E-7 bit: 58.0 Len: 29 Query Start:8 Query End:36 Subject Strand: NEGATIVE Subject Start: 142 Subject End: 170 .......tcaggctttcagccctccgctgagggggt.................................................................................................................................................................................................................................................................................................................................................................................................................... .......tcaggctttcagccctccgctgagggggt.................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF2922_TF2923|surface antigen BspA:hypothetical protein|POSITIVE:POSITIVE|[3121599,3122307]|709 HSP 1 e-value: 3.0E-10 bit: 67.9 Len: 42 Query Start:130 Query End:171 Subject Strand: POSITIVE Subject Start: 378 Subject End: 419 .................................................................................................................................tacatgattcgacgtgtaccacgcataacaattgaccattaa............................................................................................................................................................................................................................................................................. .................................................................................................................................tacatgattcggcgtgtaccacgcataacaattaaccattaa............................................................................................................................................................................................................................................................................. HSP 2 e-value: 2.0E-8 bit: 61.9 Len: 35 Query Start:1 Query End:35 Subject Strand: POSITIVE Subject Start: 13 Subject End: 47 tattcattcaggctttcagccctccgctgaggggg..................................................................................................................................................................................................................................................................................................................................................................................................................... tattcattcaggctttcagccctccgccgaggggg..................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF0685_TF0686|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[731193,732338]|1146 HSP 1 e-value: 4.0E-9 bit: 63.9 Len: 36 Query Start:142 Query End:177 Subject Strand: POSITIVE Subject Start: 720 Subject End: 755 .............................................................................................................................................cgtgtaccacgcataacaattgaccattaaccatta....................................................................................................................................................................................................................................................................... .............................................................................................................................................cgtgtaccacgcataacaattgacaattaaccatta....................................................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-6 bit: 56.0 Len: 36 Query Start:59 Query End:94 Subject Strand: POSITIVE Subject Start: 832 Subject End: 867 ..........................................................cattgggctgaattaacacgccctttcagggctgtt.......................................................................................................................................................................................................................................................................................................................................................... ..........................................................cattgggctgaattaatgcgccctttcagggctgtt.......................................................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF0179_TF0180|pyrophosphate-energized proton pump:carboxynorspermidine decarboxylase|POSITIVE:POSITIVE|[190752,191406]|655 HSP 1 e-value: 3.0E-7 bit: 58.0 Len: 64 Query Start:8 Query End:71 Subject Strand: POSITIVE Subject Start: 209 Subject End: 273 .......tcaggctttcagccctccgctgagggg-gttgccttgtttcccaacgctttgcattgggctgaat................................................................................................................................................................................................................................................................................................................................................................................. .......tcaggcttacagccctccgccgagacgcgatgccttttttcccaacgcttcgcattgggctgaat................................................................................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 1.0E-6 bit: 56.0 Len: 36 Query Start:123 Query End:158 Subject Strand: POSITIVE Subject Start: 316 Subject End: 351 ..........................................................................................................................tgccgggtacatgattcgacgtgtaccacgcataac.......................................................................................................................................................................................................................................................................................... ..........................................................................................................................tgccgggtacatgattcggcgtgtaacacgcataac.......................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUUCAUUCAGGCUUUCAGCCCUCCGCUGAGGGGGUUGCCUUGUUUCCCAACGCUUUGCAUUGGGCUGAAUUAACACGCCCUUUCAGGGCUGUUUUCCAAUUGUUAGUUGUUAAUGGUUAUUUGCCGGGUACAUGAUUCGACGUGUACCACGCAUAACAAUUGACCAUUAACCAUUAUCACCGCUCGCACGGGGCACAAGGGCUGCAAGCCCGAUAUAUUUCAGCCCCAUCCCGCAAGGGUGGGGUAUCGAUUCCCAUACCCCGUUUGGAGGGCUGAAAGCCUGAUAUAAUCGUUGGCAAUCAGUAGUUUGCCCGUUAUCACACACCGCCCAGGGGGAGAUGUCUGUCCGAGAAAUGUGCUUUCCGGCCGCCGCUGCGGCUUCGGAGUGCAAGGUAGAGACGCCCUUAAACGGGCGUCUCUACACCUCAUGCUACAUUCA .......(((((((((((((((((((..((.(((((((((((((..(((((.((...)).)))))..(((..((((.((((.....)))))))))))....((.((((.(((((((((((.(((.((((((((((......)))))))).)).....))).))))))))))).)))).))........))))))))...((((.....))))...........((((((.(((....)))))))))............))))).))))))))))))))))))))).......((.((((..(((..((((.(((...................))).))))..)))...(((.....(((.((((((((((....)))))..))))).)))..((((((((((((......))))))))))))..))).))))))..... (-191.41) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAUGUAGCAUGAGGUGUAGAGACGCCCGUUUAAGGGCGUCUCUACCUUGCACUCCGAAGCCGCAGCGGCGGCCGGAAAGCACAUUUCUCGGACAGACAUCUCCCCCUGGGCGGUGUGUGAUAACGGGCAAACUACUGAUUGCCAACGAUUAUAUCAGGCUUUCAGCCCUCCAAACGGGGUAUGGGAAUCGAUACCCCACCCUUGCGGGAUGGGGCUGAAAUAUAUCGGGCUUGCAGCCCUUGUGCCCCGUGCGAGCGGUGAUAAUGGUUAAUGGUCAAUUGUUAUGCGUGGUACACGUCGAAUCAUGUACCCGGCAAAUAACCAUUAACAACUAACAAUUGGAAAACAGCCCUGAAAGGGCGUGUUAAUUCAGCCCAAUGCAAAGCGUUGGGAAACAAGGCAACCCCCUCAGCGGAGGGCUGAAAGCCUGAAUGAAUA .((((((.((...(((.((((((((((((......)))))))))))))))....((((..(((((....)))))))))..)))))))).(((..((.(((((.(((...))).))))).))......(((((.(....).)))))..))).....((((((((((((((((((..((((((((..((...(((((((((((((....))).)))))........)))))(((.....)))))..))))))))..(((.((.(......(((((((((.(.((((....((.((((((.(......).)))))))))))).).))))))))).........(((...((((((((.....)))).)))).......((((((((...))))))))...))).....).)))))...))))))))))))))))))....... (-194.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 959 250 201 222 173 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 959 250 201 222 173 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 950 59 11 182 135 tfor:TF2478|5end_hypothetical_protein_2655375..2655605_POSITIVE 924 249 201 52 5 tfor:TF2840|5end_hypothetical_protein_3031060..3031290_POSITIVE 884 250 201 59 8 tfor:TF2547|5end_hypothetical_protein_2730664..2730894_POSITIVE 883 250 201 208 159 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 877 90 44 189 142 tfor:TF2824|5end_nucleoside-transporting_protein_nupG_3014701..3014931_POSITIVE 838 90 44 198 152 tfor:TF0828|5end_hypothetical_protein_887090..887320_POSITIVE 834 90 44 188 142 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 820 90 44 115 69 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 820 90 44 192 146 tfor:TF1186|5end_hypothetical_protein_1269568..1269798_POSITIVE 785 90 44 215 168 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 779 169 121 201 153 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 717 169 123 168 123 tfor:TF2796|5end_cytochrome_D_ubiquinol_oxidase,_subunit_II_2981269..2981499_POSITIVE 714 70 21 229 179 tfor:TF1187|5end_hypothetical_protein_1269625..1269855_POSITIVE 698 90 44 122 66 tfor:TF0538|5end_hypothetical_protein_566891..567121_POSITIVE 698 90 44 115 59 tfor:TF0539|5end_hypothetical_protein_566884..567114_POSITIVE 669 59 11 65 16 tfor:TF3107|5end_hypothetical_protein_3338459..3338689_POSITIVE 661 70 21 230 181 tfor:TF0823|5end_conserved_hypothetical_protein_880660..880890_POSITIVE 648 158 111 183 131 tfor:TF1655|5end_long-chain-fatty-acid-CoA_ligase_1769279..1769509_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 823 90 44 60 14 tfor:TF0820|3end_hypothetical_protein_879942..880092_POSITIVE 820 90 44 107 61 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 789 70 21 125 75 tfor:TF2547|3end_hypothetical_protein_2730947..2731097_POSITIVE 785 90 44 69 22 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 748 110 61 101 52 tfor:TF2840|3end_hypothetical_protein_3031289..3031439_POSITIVE 669 59 11 134 85 tfor:TF3106|3end_hypothetical_protein_3338608..3338758_POSITIVE 532 59 11 141 91 tfor:TF1405|3end_conserved_hypothetical_protein_1481819..1481969_POSITIVE 530 50 6 132 86 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 520 338 292 95 36 tfor:TF0145|3end_glutamine_synthetase_154718..154868_POSITIVE 510 285 244 42 1 tfor:TF0677|3end_ATP-binding_protein,_Mrp/Nbp35_family,_involved_in_chromosome_partitioning_720982..721132_POSITIVE 508 140 98 130 88 tfor:TF1762|3end_hypothetical_protein_1899648..1899798_POSITIVE 508 140 98 61 19 tfor:TF1761|3end_hypothetical_protein_1899579..1899729_POSITIVE 489 330 282 76 23 tfor:TF0795|3end_hypothetical_protein_853199..853349_POSITIVE 487 285 242 53 19 tfor:TF0018|3end_hypothetical_protein_12091..12241_POSITIVE 485 285 242 71 38 tfor:TF2922|3end_surface_antigen_BspA_3121538..3121688_POSITIVE 480 285 241 74 32 tfor:TF2350|3end_hypothetical_protein_2515364..2515514_POSITIVE 480 405 363 44 4 tfor:TF0037|3end_sialic_acid-specific_9-O-acetylesterase_39991..40141_POSITIVE 477 90 41 52 3 tfor:TF0533|3end_hypothetical_protein_559519..559669_POSITIVE 471 404 365 45 5 tfor:TF1818|3end_conserved_hypothetical_protein_1958754..1958904_POSITIVE 466 120 79 42 1 tfor:TF1848|3end_conserved_hypothetical_protein_1997944..1998094_POSITIVE