Origin IGS: aaagatacaacataaagtttattcaacagatgtttcaggttttattcatccgatttcttttcatatttagagcccctatagaatcataaaagattggtttatgcctacgcttaagattagaaaaagaaaccatgtaaaatatcaaaagaaataaatcagaatgagatgtaccatacatcaaaaatcctatagcacatctcattcacattgtttaaaagagggactacaagcagaacaaaaataagagaacatactctttcatcttttttgctctactttctacccaatgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacattgggtagaaagtagagcaaaaaagatgaaagagtatgttctcttatttttgttctgcttgtagtccctcttttaaacaatgtgaatgagatgtgctataggatttttgatgtatggtacatctcattctgatttatttcttttgatattttacatggtttctttttctaatcttaagcgtaggcataaaccaatcttttatgattctataggggctctaaatatgaaaagaaatcggatgaataaaacctgaaacatctgttgaataaactttatgttgtatcttt Mask Tandem Repeat Region ================================================ aaagatacaacataaagtttattcaacagatgtttcaggttttattcatccgatttcttttcatatttagagcccctatagaatcataaaagattggtttatgcctacgcttaagattagaaaaagaaaccatgtaaaatatcaaaagaaataaatcagaatgagatgtaccatacatcaaaaatcctatagcacatctcattcacattgtttaaaagagggactacaagcagaacaaaaataagagaacatactctttcatcttttttgctctactttctacccaatgtta Find is-nt database================================================ Query_seq: TF1565:TF1566|TF1565:TF1566:polysaccharide export protein, BexD/CtrA/VexA family:H+-transporting ATP synthase, subunit K:->->:1673807..1674098 292 aaagatacaacataaagtttattcaacagatgtttcaggttttattcatccgatttcttttcatatttagagcccctatagaatcataaaagattggtttatgcctacgcttaagattagaaaaagaaaccatgtaaaatatcaaaagaaataaatcagaatgagatgtaccatacatcaaaaatcctatagcacatctcattcacattgtttaaaagagggactacaagcagaacaaaaataagagaacatactctttcatcttttttgctctactttctacccaatgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1565:TF1566|TF1565:TF1566:polysaccharide export protein, BexD/CtrA/VexA family:H+-transporting ATP synthase, subunit K:->->:1673807..1674098 292 aaagatacaacataaagtttattcaacagatgtttcaggttttattcatccgatttcttttcatatttagagcccctatagaatcataaaagattggtttatgcctacgcttaagattagaaaaagaaaccatgtaaaatatcaaaagaaataaatcagaatgagatgtaccatacatcaaaaatcctatagcacatctcattcacattgtttaaaagagggactacaagcagaacaaaaataagagaacatactctttcatcttttttgctctactttctacccaatgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1565:TF1566|TF1565:TF1566:polysaccharide export protein, BexD/CtrA/VexA family:H+-transporting ATP synthase, subunit K:->->:1673807..1674098 292 aaagatacaacataaagtttattcaacagatgtttcaggttttattcatccgatttcttttcatatttagagcccctatagaatcataaaagattggtttatgcctacgcttaagattagaaaaagaaaccatgtaaaatatcaaaagaaataaatcagaatgagatgtaccatacatcaaaaatcctatagcacatctcattcacattgtttaaaagagggactacaagcagaacaaaaataagagaacatactctttcatcttttttgctctactttctacccaatgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaagatacaacataaagtttattcaacagatgtttcaggttttattcatccgatttcttttcatatttagagcccctatagaatcataaaagattggtttatgcctacgcttaagattagaaaaagaaaccatgtaaaatatcaaaagaaataaatcagaatgagatgtaccatacatcaaaaatcctatagcacatctcattcacattgtttaaaagagggactacaagcagaacaaaaataagagaacatactctttcatcttttttgctctactttctacccaatgtta Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 196 End: 291 ................................................................................................................................................................................................... M S F T L F K R G T T S R T K I R E H T L S S F L L Y F L P N V . Protein_Len: 55 Strand: - Start: 38 End: 202 ..................................... T K N M R N R K M N L A G I S D Y F I P K H R R K L N S F S V M Y F I L L F L D S H S T G Y M L F G I A C R M .......................................................................................... aaagatacaacataaagtttattcaacagatgtttcaggttttattcatccgatttcttttcatatttagagcccctatagaatcataaaagattggtttatgcctacgcttaagattagaaaaagaaaccatgtaaaatatcaaaagaaataaatcagaatgagatgtaccatacatcaaaaatcctatagcacatctcattcacattgtttaaaagagggactacaagcagaacaaaaataagagaacatactctttcatcttttttgctctactttctacccaatgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaagatacaacataaagtttattcaacagatgtttcaggttttattcatccgatttcttttcatatttagagcccctatagaatcataaaagattggtttatgcctacgcttaagattagaaaaagaaaccatgtaaaatatcaaaagaaataaatcagaatgagatgtaccatacatcaaaaatcctatagcacatctcattcacattgtttaaaagagggactacaagcagaacaaaaataagagaacatactctttcatcttttttgctctactttctacccaatgtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1565:TF1566|TF1565:TF1566:polysaccharide export protein, BexD/CtrA/VexA family:H+-transporting ATP synthase, subunit K:->->:1673807..1674098 292 aaagatacaacataaagtttattcaacagatgtttcaggttttattcatccgatttcttttcatatttagagcccctatagaatcataaaagattggtttatgcctacgcttaagattagaaaaagaaaccatgtaaaatatcaaaagaaataaatcagaatgagatgtaccatacatcaaaaatcctatagcacatctcattcacattgtttaaaagagggactacaagcagaacaaaaataagagaacatactctttcatcttttttgctctactttctacccaatgtta Intra-Species Hit: Count: 1 Min: 1 Max: 292 Len: 292 Subject: tfor_TF1565_TF1566|polysaccharide export protein, BexD/CtrA/VexA family:H+-transporting ATP synthase, subunit K|POSITIVE:POSITIVE|[1673807,1674098]|292 HSP 1 e-value: 1.0E-164 bit: 579.0 Len: 292 Query Start:1 Query End:292 Subject Strand: POSITIVE Subject Start: 1 Subject End: 292 aaagatacaacataaagtttattcaacagatgtttcaggttttattcatccgatttcttttcatatttagagcccctatagaatcataaaagattggtttatgcctacgcttaagattagaaaaagaaaccatgtaaaatatcaaaagaaataaatcagaatgagatgtaccatacatcaaaaatcctatagcacatctcattcacattgtttaaaagagggactacaagcagaacaaaaataagagaacatactctttcatcttttttgctctactttctacccaatgtta aaagatacaacataaagtttattcaacagatgtttcaggttttattcatccgatttcttttcatatttagagcccctatagaatcataaaagattggtttatgcctacgcttaagattagaaaaagaaaccatgtaaaatatcaaaagaaataaatcagaatgagatgtaccatacatcaaaaatcctatagcacatctcattcacattgtttaaaagagggactacaagcagaacaaaaataagagaacatactctttcatcttttttgctctactttctacccaatgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAGAUACAACAUAAAGUUUAUUCAACAGAUGUUUCAGGUUUUAUUCAUCCGAUUUCUUUUCAUAUUUAGAGCCCCUAUAGAAUCAUAAAAGAUUGGUUUAUGCCUACGCUUAAGAUUAGAAAAAGAAACCAUGUAAAAUAUCAAAAGAAAUAAAUCAGAAUGAGAUGUACCAUACAUCAAAAAUCCUAUAGCACAUCUCAUUCACAUUGUUUAAAAGAGGGACUACAAGCAGAACAAAAAUAAGAGAACAUACUCUUUCAUCUUUUUUGCUCUACUUUCUACCCAAUGUUA ........(((((....((((..(((..(((((((((((((((..((....))....(((((..((((.((((........((((......))))(((....)))...)))).))))..))))).)))))).))..)))))))...............(((((((((((.(.(((............))).).)))))))))))...)))..))))...(((.....(((.(((.((((((.(((((......)))))......)))))).))).)))....))).))))). (-53.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACAUUGGGUAGAAAGUAGAGCAAAAAAGAUGAAAGAGUAUGUUCUCUUAUUUUUGUUCUGCUUGUAGUCCCUCUUUUAAACAAUGUGAAUGAGAUGUGCUAUAGGAUUUUUGAUGUAUGGUACAUCUCAUUCUGAUUUAUUUCUUUUGAUAUUUUACAUGGUUUCUUUUUCUAAUCUUAAGCGUAGGCAUAAACCAAUCUUUUAUGAUUCUAUAGGGGCUCUAAAUAUGAAAAGAAAUCGGAUGAAUAAAACCUGAAACAUCUGUUGAAUAAACUUUAUGUUGUAUCUUU ((((((.(((....((((((((((((((......(((((......))))).)))))))))))))).....))).......(((((((((((((((((((((((((.(........).)))))))))))))))))..(((((((((((((.((((((.....(((..((......((((.((((.(..((......))...).)))).))))....))..)))..)))))).))))))))).))))..............)))).))))...........))))))....... (-73.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 279 77 31 127 68 tfor:TF1009|5end_conserved_hypothetical_protein;_posible_rod-shape_determining_protein,_MreD_1064290..1064520_POSITIVE 245 80 51 130 98 tfor:TF0586|5end_conserved_hypothetical_protein_617313..617543_POSITIVE 241 285 244 221 187 tfor:TF2653|5end_hypothetical_protein_2822093..2822323_POSITIVE 238 267 225 227 191 tfor:TF1737|5end_outer_membrane_protein,_TonB_dependent_receptor_1861834..1862064_POSITIVE 238 267 225 76 40 tfor:TF1736|5end_hypothetical_protein_1861683..1861913_POSITIVE 237 77 33 221 175 tfor:TF0483|5end_outer_membrane_receptor_504034..504264_POSITIVE 229 285 250 54 19 tfor:TF2511|5end_RNA_binding_protein_2689910..2690140_POSITIVE 228 221 183 192 159 tfor:TF0184|5end_dihydroneopterin_aldolase_195629..195859_POSITIVE 222 284 253 218 185 tfor:TF2627|5end_SAM-dependent_methyltransferase_2799910..2800140_POSITIVE 220 238 191 96 49 tfor:TF1705|5end_conserved_hypothetical_protein;_possible_DNA_helicase_1827214..1827444_POSITIVE 220 286 252 207 159 tfor:TF0180|5end_carboxynorspermidine_decarboxylase_191267..191497_POSITIVE 220 77 50 194 168 tfor:TF0021|5end_outer_membrane_phospholipase_A_15949..16179_POSITIVE 219 268 242 97 71 tfor:TF2533|5end_adenylosuccinate_synthetase_2715666..2715896_POSITIVE 219 111 72 116 71 tfor:TF0394|5end_oxaloacetate_decarboxylase,_alpha_subunit_(pyruvate_carboxylase,_C-terminal_domain)_413268..413498_POSITIVE 217 198 159 220 174 tfor:TF2993|5end_SAM-dependent_methyltransferase_3209636..3209866_POSITIVE 217 87 41 153 106 tfor:TF1987|5end_hypothetical_protein_2137359..2137589_POSITIVE 217 83 42 112 67 tfor:TF1538|5end_conserved_hypothetical_protein_1643531..1643761_POSITIVE 216 112 74 101 57 tfor:TF1047|5end_tRNA_pseudouridylate_synthetase_A_(pseudouridylate_synthase_I)_1108483..1108713_POSITIVE 215 234 192 85 41 tfor:TF0607|5end_DnaK_suppressor_protein_637441..637671_POSITIVE 214 280 254 57 23 tfor:TF1542|5end_transcriptional_regulator_1649105..1649335_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 279 77 31 131 72 tfor:TF1008|3end_rod_shape-determining_protein_MreC_1064374..1064524_POSITIVE 241 285 244 62 28 tfor:TF2651|3end_hypothetical_protein_2822014..2822164_POSITIVE 224 200 169 135 103 tfor:TF0793|3end_conserved_hypothetical_protein;_possible_AAA_superfamily_ATPase_851291..851441_POSITIVE 222 284 253 118 85 tfor:TF2626|3end_conserved_hypothetical_protein_2799890..2800040_POSITIVE 222 107 67 49 5 tfor:TF1746|3end_histidine_ammonia-lyase_1881153..1881303_POSITIVE 220 238 191 139 92 tfor:TF1704|3end_conserved_hypothetical_protein_1827337..1827487_POSITIVE 219 268 242 59 33 tfor:TF2532|3end_ferric-uptake_regulator_2715708..2715858_POSITIVE 215 234 192 68 24 tfor:TF0606|3end_signal_peptidase_II_637504..637654_POSITIVE 213 285 243 46 7 tfor:TF0828|3end_hypothetical_protein_887508..887658_POSITIVE 211 205 161 63 26 tfor:TF0889|3end_conjugative_transposon_protein,_TraO_940078..940228_POSITIVE 211 71 34 139 95 tfor:TF0548|3end_TPR-repeat-containing_protein_581417..581567_POSITIVE 210 88 48 80 44 tfor:TF2126|3end_S-adenosylhomocysteine_hydrolase_2303996..2304146_POSITIVE 209 74 36 46 1 tfor:TF3143|3end_conserved_hypothetical_protein;_possible_membrane_protein_3379227..3379377_POSITIVE 208 65 22 121 75 tfor:TF2858|3end_hypothetical_protein_3044874..3045024_POSITIVE 206 196 163 51 10 tfor:TF2813|3end_possible_flavoprotein_A_3004540..3004690_POSITIVE 205 109 70 126 93 tfor:TF2855|3end_ATPase,_AAA_family_3042011..3042161_POSITIVE 204 129 83 143 85 tfor:TF1959|3end_conserved_hypothetical_protein_2110506..2110656_POSITIVE 204 286 242 104 56 tfor:TF1739|3end_possible_sulfatase_1868604..1868754_POSITIVE 202 65 28 103 60 tfor:TF2767|3end_2-oxoglutarate_synthase,_alpha_subunit_2954151..2954301_POSITIVE 202 121 88 47 11 tfor:TF2282|3end_hypothetical_protein_2439822..2439972_POSITIVE