Origin IGS: taaagactgaaagcaacgtgtctgaagcaacgattcgttttcattgcatatctttgcagagattaattgccaatacccggtgcagatataggctgggtagggtgttttctgtcggttctataagaca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgtcttatagaaccgacagaaaacaccctacccagcctatatctgcaccgggtattggcaattaatctctgcaaagatatgcaatgaaaacgaatcgttgcttcagacacgttgctttcagtcttta Mask Tandem Repeat Region ================================================ taaagactgaaagcaacgtgtctgaagcaacgattcgttttcattgcatatctttgcagagattaattgccaatacccggtgcagatataggctgggtagggtgttttctgtcggttctataagaca Find is-nt database================================================ Query_seq: TF1536:TF1538|TF1536:TF1538:hypothetical protein:conserved hypothetical protein:->->:1643544..1643670 127 taaagactgaaagcaacgtgtctgaagcaacgattcgttttcattgcatatctttgcagagattaattgccaatacccggtgcagatataggctgggtagggtgttttctgtcggttctataagaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1536:TF1538|TF1536:TF1538:hypothetical protein:conserved hypothetical protein:->->:1643544..1643670 127 taaagactgaaagcaacgtgtctgaagcaacgattcgttttcattgcatatctttgcagagattaattgccaatacccggtgcagatataggctgggtagggtgttttctgtcggttctataagaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1536:TF1538|TF1536:TF1538:hypothetical protein:conserved hypothetical protein:->->:1643544..1643670 127 taaagactgaaagcaacgtgtctgaagcaacgattcgttttcattgcatatctttgcagagattaattgccaatacccggtgcagatataggctgggtagggtgttttctgtcggttctataagaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaagactgaaagcaacgtgtctgaagcaacgattcgttttcattgcatatctttgcagagattaattgccaatacccggtgcagatataggctgggtagggtgttttctgtcggttctataagaca Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 18 End: 125 ................. M S E A T I R F H C I S L Q R L I A N T R C R Y R L G R V F S V G S I R .. taaagactgaaagcaacgtgtctgaagcaacgattcgttttcattgcatatctttgcagagattaattgccaatacccggtgcagatataggctgggtagggtgttttctgtcggttctataagaca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaagactgaaagcaacgtgtctgaagcaacgattcgttttcattgcatatctttgcagagattaattgccaatacccggtgcagatataggctgggtagggtgttttctgtcggttctataagaca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1536:TF1538|TF1536:TF1538:hypothetical protein:conserved hypothetical protein:->->:1643544..1643670 127 taaagactgaaagcaacgtgtctgaagcaacgattcgttttcattgcatatctttgcagagattaattgccaatacccggtgcagatataggctgggtagggtgttttctgtcggttctataagaca Intra-Species Hit: Count: 1 Min: 1 Max: 127 Len: 127 Subject: tfor_TF1536_TF1538|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1643544,1643670]|127 HSP 1 e-value: 2.0E-66 bit: 252.0 Len: 127 Query Start:1 Query End:127 Subject Strand: POSITIVE Subject Start: 1 Subject End: 127 taaagactgaaagcaacgtgtctgaagcaacgattcgttttcattgcatatctttgcagagattaattgccaatacccggtgcagatataggctgggtagggtgttttctgtcggttctataagaca taaagactgaaagcaacgtgtctgaagcaacgattcgttttcattgcatatctttgcagagattaattgccaatacccggtgcagatataggctgggtagggtgttttctgtcggttctataagaca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAGACUGAAAGCAACGUGUCUGAAGCAACGAUUCGUUUUCAUUGCAUAUCUUUGCAGAGAUUAAUUGCCAAUACCCGGUGCAGAUAUAGGCUGGGUAGGGUGUUUUCUGUCGGUUCUAUAAGACA ....(((.((((((.....((((...((((.(((..((.......))..))).))))..)))).....(((..((((((((..........)))))))).))))))))).))).............. (-30.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUCUUAUAGAACCGACAGAAAACACCCUACCCAGCCUAUAUCUGCACCGGGUAUUGGCAAUUAAUCUCUGCAAAGAUAUGCAAUGAAAACGAAUCGUUGCUUCAGACACGUUGCUUUCAGUCUUUA ..............(((.((((.((.........(((.(((((((...))))))).)))................((...(((((((.......))))))).))........)).)))).))).... (-24.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 362 60 13 180 139 tfor:TF0546|5end_conserved_hypothetical_protein_576380..576610_POSITIVE 345 80 31 57 13 tfor:TF1896|5end_conserved_hypothetical_protein;_possible_phage-related_protein_2047858..2048088_POSITIVE 341 103 68 169 133 tfor:TF1906|5end_conserved_hypothetical_protein_2060955..2061185_POSITIVE 339 100 54 67 20 tfor:TF2255|5end_transfer_region-related_protein,_TraH_2427504..2427734_POSITIVE 335 113 77 77 36 tfor:TF0011|5end_conserved_hypothetical_protein_4685..4915_POSITIVE 325 104 68 190 152 tfor:TF1346|5end_hypothetical_protein_1425880..1426110_POSITIVE 324 117 72 80 17 tfor:TF1492|5end_ribonuclease_H-related_protein_1592017..1592247_POSITIVE 324 116 74 192 150 tfor:TF0544|5end_metallo-beta_lactamase_family_protein_575121..575351_POSITIVE 321 59 17 173 127 tfor:TF2159|5end_conserved_hypothetical_protein_2334446..2334676_POSITIVE 321 117 76 151 100 tfor:TF0058|5end_conserved_hypothetical_protein_59833..60063_POSITIVE 317 115 77 104 67 tfor:TF2504|5end_peptidyl-prolyl_cis-trans_isomerase_2680031..2680261_POSITIVE 316 119 71 206 153 tfor:TF3140|5end_Na+-translocating_NADH-quinone_reductase,_subunit_B_3373309..3373539_POSITIVE 316 97 52 209 167 tfor:TF2166|5end_hypothetical_protein_2342115..2342345_POSITIVE 314 119 76 213 173 tfor:TF1840|5end_transposase_1990221..1990451_POSITIVE 312 119 77 228 179 tfor:TF3109|5end_hypothetical_protein_3341998..3342228_POSITIVE 311 119 77 134 88 tfor:TF0962|5end_possible_amidohydrolase_1011593..1011823_POSITIVE 310 115 77 139 109 tfor:TF1003|5end_conserved_hypothetical_protein;_possible_secreted_glycosylhydrolase_1058177..1058407_POSITIVE 308 119 77 91 48 tfor:TF2499|5end_possible_thioesterase_family_protein_2673903..2674133_POSITIVE 308 120 71 124 76 tfor:TF1975|5end_uridylate_kinase_2121536..2121766_POSITIVE 308 115 71 171 125 tfor:TF0499|5end_hypothetical_protein_526257..526487_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 342 119 71 117 77 tfor:TF1848|3end_conserved_hypothetical_protein_1997944..1998094_POSITIVE 338 119 76 132 87 tfor:TF0370|3end_hypothetical_protein_386317..386467_POSITIVE 335 113 77 102 61 tfor:TF0010|3end_conserved_hypothetical_protein_4790..4940_POSITIVE 328 119 76 111 68 tfor:TF0821|3end_hypothetical_protein_880195..880345_POSITIVE 324 117 72 70 7 tfor:TF1491|3end_mannosyltransferase_1592087..1592237_POSITIVE 323 116 76 88 45 tfor:TF0828|3end_hypothetical_protein_887508..887658_POSITIVE 317 115 77 104 67 tfor:TF2503|3end_conserved_hypothetical_protein_2680111..2680261_POSITIVE 313 118 78 148 112 tfor:TF1253|3end_hypothetical_protein_1336211..1336361_POSITIVE 311 80 32 44 1 tfor:TF1895|3end_hypothetical_protein_2047925..2048075_POSITIVE 311 119 77 134 88 tfor:TF0961|3end_translation_initiation_inhibitor,_endoribonuclease_activity_1011673..1011823_POSITIVE 310 115 77 114 84 tfor:TF1002|3end_replicative_DNA_helicase_1058232..1058382_POSITIVE 306 115 78 57 15 tfor:TF2808|3end_bacterial_sugar_transferase_2999909..3000059_POSITIVE 306 119 73 148 99 tfor:TF0959|3end_periplasmic_protease_1008540..1008690_POSITIVE 305 119 77 82 41 tfor:TF0422|3end_proline_dehydrogenase_438695..438845_POSITIVE 304 119 74 60 8 tfor:TF2110|3end_transposase_2280526..2280676_POSITIVE 304 109 67 70 29 tfor:TF1917|3end_hypothetical_protein_2070244..2070394_POSITIVE 300 117 71 148 97 tfor:TF0777|3end_1,4-dihydroxy-2-naphthoate_octaprenyltransferase_827053..827203_POSITIVE 297 100 54 150 91 tfor:TF2646|3end_conserved_hypothetical_protein_2816697..2816847_POSITIVE 295 117 74 55 3 tfor:TF1433|3end_ribose_5-phosphate_isomerase_1517551..1517701_POSITIVE 295 119 73 83 38 tfor:TF1249|3end_phosphoglycerate_kinase_(cytosolic_phosphoglycerate_kinase)_1332356..1332506_POSITIVE