Origin IGS: acaaaattattacgttagcgatcggataaaccgtaagtttcactcatttaatggttaatggttattctttactaccttgacgaccttgttactttttttcattttaccacaagttttcattcagcggtcagcattcgtcattcttcgtttttctgtttttagtctatcagccaaacaaatcaacatccaaaacaatcattacatcggcttacaaccctgcgccgagatgtgtaccctttttctcccaacgcttcgcattgagctgaattaacacacctctatttattaattgttaattgtcatgcagggtacatgattggggctgtaccacgcataaccattgacgattaaacattaacagttaaccatcgaaattatttccacctgcgaagattaaacgccccaggggcatacatgggctgaaagtccgttctatttcagccccatcccataaagggtagggtatcgattcccctgtcttgtttttgagggctgtaagcctgatgcaatttatcagttagcattcagctttcagtcaacaatcgaaacatttcaacaattaaacaactcaacattcaaatgcccaaagggcaggcatctaatactcaaaaggctgaaagcccgatttagtttaatggttaattgttaacggtcaagagttatcttccgggtacatgacttggcatgcattatgcatagccaactaacaatcatctatttacaattattatatcgtgtatcacgcataacaattgacaactgaccattaataatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taattattaatggtcagttgtcaattgttatgcgtgatacacgatataataattgtaaatagatgattgttagttggctatgcataatgcatgccaagtcatgtacccggaagataactcttgaccgttaacaattaaccattaaactaaatcgggctttcagccttttgagtattagatgcctgccctttgggcatttgaatgttgagttgtttaattgttgaaatgtttcgattgttgactgaaagctgaatgctaactgataaattgcatcaggcttacagccctcaaaaacaagacaggggaatcgataccctaccctttatgggatggggctgaaatagaacggactttcagcccatgtatgcccctggggcgtttaatcttcgcaggtggaaataatttcgatggttaactgttaatgtttaatcgtcaatggttatgcgtggtacagccccaatcatgtaccctgcatgacaattaacaattaataaatagaggtgtgttaattcagctcaatgcgaagcgttgggagaaaaagggtacacatctcggcgcagggttgtaagccgatgtaatgattgttttggatgttgatttgtttggctgatagactaaaaacagaaaaacgaagaatgacgaatgctgaccgctgaatgaaaacttgtggtaaaatgaaaaaaagtaacaaggtcgtcaaggtagtaaagaataaccattaaccattaaatgagtgaaacttacggtttatccgatcgctaacgtaataattttgt Mask Tandem Repeat Region ================================================ acaaaattattacgttagcgatcggataaaccgtaagtttcactcatttaatggttaatggttattctttactaccttgacgaccttgttactttttttcattttaccacaagttttcattcagcggtcagcattcgtcattcttcgtttttctgtttttagtctatcagccaaacaaatcaacatccaaaacaatcattacatcggcttacaaccctgcgccgagatgtgtaccctttttctcccaacgcttcgcattgagctgaattaacacacctctatttattaattgttaattgtcatgcagggtacatgattggggctgtaccacgcataaccattgacgattaaacattaacagttaaccatcgaaattatttccacctgcgaagattaaacgccccaggggcatacatgggctgaaagtccgttctatttcagccccatcccataaagggtagggtatcgattcccctgtcttgtttttgagggctgtaagcctgatgcaatttatcagttagcattcagctttcagtcaacaatcgaaacatttcaacaattaaacaactcaacattcaaatgcccaaagggcaggcatctaatactcaaaaggctgaaagcccgatttagtttaatggttaattgttaacggtcaagagttatcttccgggtacatgacttggcatgcattatgcatagccaactaacaatcatctatttacaattattatatcgtgtatcacgcataacaattgacaactgaccattaataatta Find is-nt database================================================ Query_seq: TF1535:TF1537|TF1535:TF1537:possible outer membrane receptor protein:hypothetical protein:->->:1642270..1643045 776 acaaaattattacgttagcgatcggataaaccgtaagtttcactcatttaatggttaatggttattctttactaccttgacgaccttgttactttttttcattttaccacaagttttcattcagcggtcagcattcgtcattcttcgtttttctgtttttagtctatcagccaaacaaatcaacatccaaaacaatcattacatcggcttacaaccctgcgccgagatgtgtaccctttttctcccaacgcttcgcattgagctgaattaacacacctctatttattaattgttaattgtcatgcagggtacatgattggggctgtaccacgcataaccattgacgattaaacattaacagttaaccatcgaaattatttccacctgcgaagattaaacgccccaggggcatacatgggctgaaagtccgttctatttcagccccatcccataaagggtagggtatcgattcccctgtcttgtttttgagggctgtaagcctgatgcaatttatcagttagcattcagctttcagtcaacaatcgaaacatttcaacaattaaacaactcaacattcaaatgcccaaagggcaggcatctaatactcaaaaggctgaaagcccgatttagtttaatggttaattgttaacggtcaagagttatcttccgggtacatgacttggcatgcattatgcatagccaactaacaatcatctatttacaattattatatcgtgtatcacgcataacaattgacaactgaccattaataatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1535:TF1537|TF1535:TF1537:possible outer membrane receptor protein:hypothetical protein:->->:1642270..1643045 776 acaaaattattacgttagcgatcggataaaccgtaagtttcactcatttaatggttaatggttattctttactaccttgacgaccttgttactttttttcattttaccacaagttttcattcagcggtcagcattcgtcattcttcgtttttctgtttttagtctatcagccaaacaaatcaacatccaaaacaatcattacatcggcttacaaccctgcgccgagatgtgtaccctttttctcccaacgcttcgcattgagctgaattaacacacctctatttattaattgttaattgtcatgcagggtacatgattggggctgtaccacgcataaccattgacgattaaacattaacagttaaccatcgaaattatttccacctgcgaagattaaacgccccaggggcatacatgggctgaaagtccgttctatttcagccccatcccataaagggtagggtatcgattcccctgtcttgtttttgagggctgtaagcctgatgcaatttatcagttagcattcagctttcagtcaacaatcgaaacatttcaacaattaaacaactcaacattcaaatgcccaaagggcaggcatctaatactcaaaaggctgaaagcccgatttagtttaatggttaattgttaacggtcaagagttatcttccgggtacatgacttggcatgcattatgcatagccaactaacaatcatctatttacaattattatatcgtgtatcacgcataacaattgacaactgaccattaataatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1535:TF1537|TF1535:TF1537:possible outer membrane receptor protein:hypothetical protein:->->:1642270..1643045 776 acaaaattattacgttagcgatcggataaaccgtaagtttcactcatttaatggttaatggttattctttactaccttgacgaccttgttactttttttcattttaccacaagttttcattcagcggtcagcattcgtcattcttcgtttttctgtttttagtctatcagccaaacaaatcaacatccaaaacaatcattacatcggcttacaaccctgcgccgagatgtgtaccctttttctcccaacgcttcgcattgagctgaattaacacacctctatttattaattgttaattgtcatgcagggtacatgattggggctgtaccacgcataaccattgacgattaaacattaacagttaaccatcgaaattatttccacctgcgaagattaaacgccccaggggcatacatgggctgaaagtccgttctatttcagccccatcccataaagggtagggtatcgattcccctgtcttgtttttgagggctgtaagcctgatgcaatttatcagttagcattcagctttcagtcaacaatcgaaacatttcaacaattaaacaactcaacattcaaatgcccaaagggcaggcatctaatactcaaaaggctgaaagcccgatttagtttaatggttaattgttaacggtcaagagttatcttccgggtacatgacttggcatgcattatgcatagccaactaacaatcatctatttacaattattatatcgtgtatcacgcataacaattgacaactgaccattaataatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acaaaattattacgttagcgatcggataaaccgtaagtttcactcatttaatggttaatggttattctttactaccttgacgaccttgttactttttttcattttaccacaagttttcattcagcggtcagcattcgtcattcttcgtttttctgtttttagtctatcagccaaacaaatcaacatccaaaacaatcattacatcggcttacaaccctgcgccgagatgtgtaccctttttctcccaacgcttcgcattgagctgaattaacacacctctatttattaattgttaattgtcatgcagggtacatgattggggctgtaccacgcataaccattgacgattaaacattaacagttaaccatcgaaattatttccacctgcgaagattaaacgccccaggggcatacatgggctgaaagtccgttctatttcagccccatcccataaagggtagggtatcgattcccctgtcttgtttttgagggctgtaagcctgatgcaatttatcagttagcattcagctttcagtcaacaatcgaaacatttcaacaattaaacaactcaacattcaaatgcccaaagggcaggcatctaatactcaaaaggctgaaagcccgatttagtttaatggttaattgttaacggtcaagagttatcttccgggtacatgacttggcatgcattatgcatagccaactaacaatcatctatttacaattattatatcgtgtatcacgcataacaattgacaactgaccattaataatta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 58 End: 159 ......................................................... M V I L Y Y L D D L V T F F H F T T S F H S A V S I R H S S F F C F ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: + Start: 166 End: 258 ..................................................................................................................................................................... M S Q T N Q H P K Q S L H R L T T L R R D V Y P F S P N A S H ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 37 Strand: + Start: 153 End: 263 ........................................................................................................................................................ M F L V Y Q P N K S T S K T I I T S A Y N P A P R C V P F F S Q R F A L S ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 77 End: 268 ............................................................................ M T T L L L F F I L P Q V F I Q R S A F V I L R F S V F S L S A K Q I N I Q N N H Y I G L Q P C A E M C T L F L P T L R ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 35 Strand: + Start: 523 End: 627 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M Q L S V N N R N I S T I K Q L N I Q M P K G Q A S N T Q K A E S P I ..................................................................................................................................................... Protein_Len: 46 Strand: + Start: 509 End: 646 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M Y Q L A F S F Q S T I E T F Q Q L N N S T F K C P K G R H L I L K R L K A R F S L M V N C .................................................................................................................................. Protein_Len: 33 Strand: + Start: 662 End: 760 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M F R V H D L A C I M H S Q L T I I Y L Q L L Y R V S R I T I D N ................ Protein_Len: 51 Strand: - Start: 365 End: 517 ............................................................................................................................................................................................................................................................................................................................................................................ G D F N N G G A F I L R G L P M C P S F T R E I E A G D W L P Y P I S E G T K N K L A T L R I C N I M ................................................................................................................................................................................................................................................................... acaaaattattacgttagcgatcggataaaccgtaagtttcactcatttaatggttaatggttattctttactaccttgacgaccttgttactttttttcattttaccacaagttttcattcagcggtcagcattcgtcattcttcgtttttctgtttttagtctatcagccaaacaaatcaacatccaaaacaatcattacatcggcttacaaccctgcgccgagatgtgtaccctttttctcccaacgcttcgcattgagctgaattaacacacctctatttattaattgttaattgtcatgcagggtacatgattggggctgtaccacgcataaccattgacgattaaacattaacagttaaccatcgaaattatttccacctgcgaagattaaacgccccaggggcatacatgggctgaaagtccgttctatttcagccccatcccataaagggtagggtatcgattcccctgtcttgtttttgagggctgtaagcctgatgcaatttatcagttagcattcagctttcagtcaacaatcgaaacatttcaacaattaaacaactcaacattcaaatgcccaaagggcaggcatctaatactcaaaaggctgaaagcccgatttagtttaatggttaattgttaacggtcaagagttatcttccgggtacatgacttggcatgcattatgcatagccaactaacaatcatctatttacaattattatatcgtgtatcacgcataacaattgacaactgaccattaataatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ PromScan Matrix: RpoN Strand: + Score: 85 Start: 680 End: 696 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTGGCATGCATTATGCA................................................................................ acaaaattattacgttagcgatcggataaaccgtaagtttcactcatttaatggttaatggttattctttactaccttgacgaccttgttactttttttcattttaccacaagttttcattcagcggtcagcattcgtcattcttcgtttttctgtttttagtctatcagccaaacaaatcaacatccaaaacaatcattacatcggcttacaaccctgcgccgagatgtgtaccctttttctcccaacgcttcgcattgagctgaattaacacacctctatttattaattgttaattgtcatgcagggtacatgattggggctgtaccacgcataaccattgacgattaaacattaacagttaaccatcgaaattatttccacctgcgaagattaaacgccccaggggcatacatgggctgaaagtccgttctatttcagccccatcccataaagggtagggtatcgattcccctgtcttgtttttgagggctgtaagcctgatgcaatttatcagttagcattcagctttcagtcaacaatcgaaacatttcaacaattaaacaactcaacattcaaatgcccaaagggcaggcatctaatactcaaaaggctgaaagcccgatttagtttaatggttaattgttaacggtcaagagttatcttccgggtacatgacttggcatgcattatgcatagccaactaacaatcatctatttacaattattatatcgtgtatcacgcataacaattgacaactgaccattaataatta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 72 HP_score: -6.9 Tail_Score: -3.92324 Start: 399 End: 411 Full_Region: ctttcagcccatgta tgccc ctg gggcg tttaatcttcgcagg ..............................................................................................................................................................................................................................................................................................................................................................................................................tgcccctggggcg............................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 45 HP_score: -3.6 Tail_Score: -2.88544 Start: 416 End: 445 Full_Region: taccctttatgggat ggggctgaaa tagaacggac tttcagccca tgtatgcccctgggg ...............................................................................................................................................................................................................................................................................................................................................................................................................................ggggctgaaatagaacggactttcagccca........................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 76 HP_score: -9.1 Tail_Score: -3.77489 Start: 581 End: 593 Full_Region: tgagtattagatgcc tgccc ttt gggca tttgaatgttgagtt ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgccctttgggca....................................................................................................................................................................................... TransTerm Strand: - Conf: 73 HP_score: -6.8 Tail_Score: -4.28656 Start: 612 End: 622 Full_Region: cattaaactaaatcg ggct ttc agcc ttttgagtattagat ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ggctttcagcc.......................................................................................................................................................... Find igs database================================================ Query_seq: TF1535:TF1537|TF1535:TF1537:possible outer membrane receptor protein:hypothetical protein:->->:1642270..1643045 776 acaaaattattacgttagcgatcggataaaccgtaagtttcactcatttaatggttaatggttattctttactaccttgacgaccttgttactttttttcattttaccacaagttttcattcagcggtcagcattcgtcattcttcgtttttctgtttttagtctatcagccaaacaaatcaacatccaaaacaatcattacatcggcttacaaccctgcgccgagatgtgtaccctttttctcccaacgcttcgcattgagctgaattaacacacctctatttattaattgttaattgtcatgcagggtacatgattggggctgtaccacgcataaccattgacgattaaacattaacagttaaccatcgaaattatttccacctgcgaagattaaacgccccaggggcatacatgggctgaaagtccgttctatttcagccccatcccataaagggtagggtatcgattcccctgtcttgtttttgagggctgtaagcctgatgcaatttatcagttagcattcagctttcagtcaacaatcgaaacatttcaacaattaaacaactcaacattcaaatgcccaaagggcaggcatctaatactcaaaaggctgaaagcccgatttagtttaatggttaattgttaacggtcaagagttatcttccgggtacatgacttggcatgcattatgcatagccaactaacaatcatctatttacaattattatatcgtgtatcacgcataacaattgacaactgaccattaataatta Intra-Species Hit: Count: 5 Min: 1 Max: 776 Len: 776 Subject: tfor_TF1535_TF1537|possible outer membrane receptor protein:hypothetical protein|POSITIVE:POSITIVE|[1642270,1643045]|776 HSP 1 e-value: 0.0 bit: 1497.0 Len: 776 Query Start:1 Query End:776 Subject Strand: POSITIVE Subject Start: 1 Subject End: 776 acaaaattattacgttagcgatcggataaaccgtaagtttcactcatttaatggttaatggttattctttactaccttgacgaccttgttacnnnnnnncattttaccacaagttttcattcagcggtcagcattcgtcattcttcgtttttctgtttttagtctatcagccaaacaaatcaacatccaaaacaatcattacatcggcttacaaccctgcgccgagatgtgtaccctttttctcccaacgcttcgcattgagctgaattaacacacctctatttattaattgttaattgtcatgcagggtacatgattggggctgtaccacgcataaccattgacgattaaacattaacagttaaccatcgaaattatttccacctgcgaagattaaacgccccaggggcatacatgggctgaaagtccgttctatttcagccccatcccataaagggtagggtatcgattcccctgtcttgtttttgagggctgtaagcctgatgcaatttatcagttagcattcagctttcagtcaacaatcgaaacatttcaacaattaaacaactcaacattcaaatgcccaaagggcaggcatctaatactcaaaaggctgaaagcccgatttagtttaatggttaattgttaacggtcaagagttatcttccgggtacatgacttggcatgcattatgcatagccaactaacaatcatctatttacaattattatatcgtgtatcacgcataacaattgacaactgaccattaataatta acaaaattattacgttagcgatcggataaaccgtaagtttcactcatttaatggttaatggttattctttactaccttgacgaccttgttactttttttcattttaccacaagttttcattcagcggtcagcattcgtcattcttcgtttttctgtttttagtctatcagccaaacaaatcaacatccaaaacaatcattacatcggcttacaaccctgcgccgagatgtgtaccctttttctcccaacgcttcgcattgagctgaattaacacacctctatttattaattgttaattgtcatgcagggtacatgattggggctgtaccacgcataaccattgacgattaaacattaacagttaaccatcgaaattatttccacctgcgaagattaaacgccccaggggcatacatgggctgaaagtccgttctatttcagccccatcccataaagggtagggtatcgattcccctgtcttgtttttgagggctgtaagcctgatgcaatttatcagttagcattcagctttcagtcaacaatcgaaacatttcaacaattaaacaactcaacattcaaatgcccaaagggcaggcatctaatactcaaaaggctgaaagcccgatttagtttaatggttaattgttaacggtcaagagttatcttccgggtacatgacttggcatgcattatgcatagccaactaacaatcatctatttacaattattatatcgtgtatcacgcataacaattgacaactgaccattaataatta Subject: tfor_TF0585_TF0586|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[616824,617452]|629 HSP 1 e-value: 1.0E-19 bit: 99.6 Len: 137 Query Start:206 Query End:342 Subject Strand: NEGATIVE Subject Start: 313 Subject End: 449 .............................................................................................................................................................................................................ggcttacaaccctgcgccgagatgtgtaccctttttctcccaacgcttcgcattgagctgaattaacacacctc-tatttattaattgttaattgtcatgcagggtacatgattggggctgtaccacgcataaccatt.................................................................................................................................................................................................................................................................................................................................................................................................................................................. .............................................................................................................................................................................................................ggcttacagccctccgccgaaatgtgaagcctttttc-cccaacgcttcgcattgggctgaatgatctcgccccctatttattaatggttaattgtcatgccgggtacatgattcggcgtgtaacacacataaccatt.................................................................................................................................................................................................................................................................................................................................................................................................................................................. Subject: tfor_TF1715_TF1716|thermolysin; zinc metalloprotease:hypothetical protein|POSITIVE:POSITIVE|[1837263,1837581]|319 HSP 1 e-value: 9.0E-18 bit: 93.7 Len: 67 Query Start:290 Query End:356 Subject Strand: NEGATIVE Subject Start: 222 Subject End: 288 .................................................................................................................................................................................................................................................................................................ttgttaattgtcatgcagggtacatgattggggctgtaccacgcataaccattgacgattaaacatt.................................................................................................................................................................................................................................................................................................................................................................................................................................... .................................................................................................................................................................................................................................................................................................ttgttaattgtcatgtagggtacatgatttggtctgtaccacgcataaccattgactattaaccatt.................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-10 bit: 69.9 Len: 55 Query Start:396 Query End:450 Subject Strand: NEGATIVE Subject Start: 156 Subject End: 210 ...........................................................................................................................................................................................................................................................................................................................................................................................................aaacgccccaggggcatacatgggctgaaagtccgttctatttcagccccatccc...................................................................................................................................................................................................................................................................................................................................... ...........................................................................................................................................................................................................................................................................................................................................................................................................aaacgccccaagggcatacaagggctgtaagcccgttttatttcagccccatccc...................................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF2161_TF2162|conserved hypothetical protein:thermolysin precursor|POSITIVE:POSITIVE|[2338423,2338808]|386 HSP 1 e-value: 5.0E-13 bit: 77.8 Len: 55 Query Start:396 Query End:450 Subject Strand: POSITIVE Subject Start: 149 Subject End: 203 ...........................................................................................................................................................................................................................................................................................................................................................................................................aaacgccccaggggcatacatgggctgaaagtccgttctatttcagccccatccc...................................................................................................................................................................................................................................................................................................................................... ...........................................................................................................................................................................................................................................................................................................................................................................................................aaacgccccaagggcatacaagggctgaaagcccgttctgtttcagccccatccc...................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 8.0E-6 bit: 54.0 Len: 60 Query Start:286 Query End:345 Subject Strand: POSITIVE Subject Start: 79 Subject End: 140 .............................................................................................................................................................................................................................................................................................ttaattgttaattgtcatgcagggtacatgattggggctgtaccacgc--ataaccattgac............................................................................................................................................................................................................................................................................................................................................................................................................................................... .............................................................................................................................................................................................................................................................................................ttaattgtcaattatcatgcagagtacatgatttggtctgtaccacacgtataaccattgac............................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF1473_TF1474|hypothetical protein:6- phosphogluconolactonase|POSITIVE:POSITIVE|[1567027, 1567481]|455 HSP 1 e-value: 5.0E-13 bit: 77.8 Len: 106 Query Start:237 Query End:342 Subject Strand: POSITIVE Subject Start: 141 Subject End: 249 ............................................................................................................................................................................................................................................tttttctcccaacgcttcgcattgagctgaattaacacacctc---tatttattaattgttaattgtcatgcagggtacatgattggggctgtaccacgcataaccatt.................................................................................................................................................................................................................................................................................................................................................................................................................................................. ............................................................................................................................................................................................................................................tttttcccccaacgcttcgcattgggctgaatgatctccccccccctatttattaatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccatt.................................................................................................................................................................................................................................................................................................................................................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACAAAAUUAUUACGUUAGCGAUCGGAUAAACCGUAAGUUUCACUCAUUUAAUGGUUAAUGGUUAUUCUUUACUACCUUGACGACCUUGUUACUUUUUUUCAUUUUACCACAAGUUUUCAUUCAGCGGUCAGCAUUCGUCAUUCUUCGUUUUUCUGUUUUUAGUCUAUCAGCCAAACAAAUCAACAUCCAAAACAAUCAUUACAUCGGCUUACAACCCUGCGCCGAGAUGUGUACCCUUUUUCUCCCAACGCUUCGCAUUGAGCUGAAUUAACACACCUCUAUUUAUUAAUUGUUAAUUGUCAUGCAGGGUACAUGAUUGGGGCUGUACCACGCAUAACCAUUGACGAUUAAACAUUAACAGUUAACCAUCGAAAUUAUUUCCACCUGCGAAGAUUAAACGCCCCAGGGGCAUACAUGGGCUGAAAGUCCGUUCUAUUUCAGCCCCAUCCCAUAAAGGGUAGGGUAUCGAUUCCCCUGUCUUGUUUUUGAGGGCUGUAAGCCUGAUGCAAUUUAUCAGUUAGCAUUCAGCUUUCAGUCAACAAUCGAAACAUUUCAACAAUUAAACAACUCAACAUUCAAAUGCCCAAAGGGCAGGCAUCUAAUACUCAAAAGGCUGAAAGCCCGAUUUAGUUUAAUGGUUAAUUGUUAACGGUCAAGAGUUAUCUUCCGGGUACAUGACUUGGCAUGCAUUAUGCAUAGCCAACUAACAAUCAUCUAUUUACAAUUAUUAUAUCGUGUAUCACGCAUAACAAUUGACAACUGACCAUUAAUAAUUA ....((((((((.(((((....(((.....))).............((((((.((((((((((((.(..........(((.(((.((((...................))))))).)))......(((((((..............((((..(((............)))..))))................(((((......(((((............)))))...(((((((((............((((......))))..((((((((.................)))))))).......))))))))))))))..)))).)))..).)))))))))))).))))))..(((((((((((((((..((((((..((....(((...........((((((((((((.(((.(((((((((..........)))))))))...(((.....)))..(((........))).)))..)))))))).)))).......((((((.....))))))...)))....((((((((((.......(((....)))..........................(((((...)))))..................))))))))))..))..)))))).)))))))))))))))..(((((..(((((....(((((((((((.(((((((((.....)))).)))))............((........)).....))))))))).)).)))))..)))))..)))))...)))))))). (-194.44) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUAUUAAUGGUCAGUUGUCAAUUGUUAUGCGUGAUACACGAUAUAAUAAUUGUAAAUAGAUGAUUGUUAGUUGGCUAUGCAUAAUGCAUGCCAAGUCAUGUACCCGGAAGAUAACUCUUGACCGUUAACAAUUAACCAUUAAACUAAAUCGGGCUUUCAGCCUUUUGAGUAUUAGAUGCCUGCCCUUUGGGCAUUUGAAUGUUGAGUUGUUUAAUUGUUGAAAUGUUUCGAUUGUUGACUGAAAGCUGAAUGCUAACUGAUAAAUUGCAUCAGGCUUACAGCCCUCAAAAACAAGACAGGGGAAUCGAUACCCUACCCUUUAUGGGAUGGGGCUGAAAUAGAACGGACUUUCAGCCCAUGUAUGCCCCUGGGGCGUUUAAUCUUCGCAGGUGGAAAUAAUUUCGAUGGUUAACUGUUAAUGUUUAAUCGUCAAUGGUUAUGCGUGGUACAGCCCCAAUCAUGUACCCUGCAUGACAAUUAACAAUUAAUAAAUAGAGGUGUGUUAAUUCAGCUCAAUGCGAAGCGUUGGGAGAAAAAGGGUACACAUCUCGGCGCAGGGUUGUAAGCCGAUGUAAUGAUUGUUUUGGAUGUUGAUUUGUUUGGCUGAUAGACUAAAAACAGAAAAACGAAGAAUGACGAAUGCUGACCGCUGAAUGAAAACUUGUGGUAAAAUGAAAAAAAGUAACAAGGUCGUCAAGGUAGUAAAGAAUAACCAUUAACCAUUAAAUGAGUGAAACUUACGGUUUAUCCGAUCGCUAACGUAAUAAUUUUGU ......((((((((((((((((..(((...((((((((........(((((((..(......)..)))))))..((((.((((.....)))))))).))))))))..)))..))))))...))))))))))...(((((.(((((((....((.(((.....))).........((((((((((.......)))))))))).....))...))))))).))))).(((((.((((((..(((((..(((.....))).(((.((..((((((((.((((((((((((((..(((((...((((((.....((((....(((.....)))...(((((((((..........)))))))))..)))).)))))).((((.((((((.(((.((((.......))))))).)))))).))))..)))))....((.(((.((((((((.((((((..........)))))).)))))))).))).))............(((((((((........((((((((...))))))))...........)))))))))...).)))))))))))))))))))))(((.(((.(((..((((.(((((((..(((............)))..)))))))...))))...(((((.((..(((.(((...(((((...................))))).)))))).)))))))........)))..))).)))..)).)))........)))))....))))))..)))))........... (-228.22) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 736 270 221 175 127 tfor:TF1186|5end_hypothetical_protein_1269568..1269798_POSITIVE 736 270 221 171 123 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 731 260 211 222 174 tfor:TF1187|5end_hypothetical_protein_1269625..1269855_POSITIVE 718 340 291 173 124 tfor:TF1716|5end_hypothetical_protein_1837442..1837672_POSITIVE 706 270 221 60 13 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 678 469 421 223 176 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 668 270 221 198 150 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 665 256 211 65 21 tfor:TF3107|5end_hypothetical_protein_3338459..3338689_POSITIVE 653 280 235 181 137 tfor:TF2824|5end_nucleoside-transporting_protein_nupG_3014701..3014931_POSITIVE 636 340 292 185 137 tfor:TF1655|5end_long-chain-fatty-acid-CoA_ligase_1769279..1769509_POSITIVE 635 469 424 60 14 tfor:TF2547|5end_hypothetical_protein_2730664..2730894_POSITIVE 612 340 291 159 111 tfor:TF2796|5end_cytochrome_D_ubiquinol_oxidase,_subunit_II_2981269..2981499_POSITIVE 603 345 303 197 155 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 603 345 303 145 103 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 571 459 417 44 4 tfor:TF2840|5end_hypothetical_protein_3031060..3031290_POSITIVE 565 340 291 185 127 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 535 350 307 170 124 tfor:TF1260|5end_chaperone_protein_DnaJ_1345325..1345555_POSITIVE 527 270 221 201 150 tfor:TF2478|5end_hypothetical_protein_2655375..2655605_POSITIVE 522 270 222 181 133 tfor:TF0828|5end_hypothetical_protein_887090..887320_POSITIVE 502 268 222 231 185 tfor:TF0823|5end_conserved_hypothetical_protein_880660..880890_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 782 260 211 118 70 tfor:TF2547|3end_hypothetical_protein_2730947..2731097_POSITIVE 736 270 221 90 42 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 668 270 221 52 4 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 665 256 211 134 90 tfor:TF3106|3end_hypothetical_protein_3338608..3338758_POSITIVE 626 345 303 142 100 tfor:TF0820|3end_hypothetical_protein_879942..880092_POSITIVE 517 340 291 142 92 tfor:TF2840|3end_hypothetical_protein_3031289..3031439_POSITIVE 447 506 461 76 34 tfor:TF2350|3end_hypothetical_protein_2515364..2515514_POSITIVE 441 518 471 98 52 tfor:TF1762|3end_hypothetical_protein_1899648..1899798_POSITIVE 439 569 523 49 4 tfor:TF2839|3end_ATP-dependent_exodeoxyribonuclease_3031035..3031185_POSITIVE 416 653 612 88 40 tfor:TF0018|3end_hypothetical_protein_12091..12241_POSITIVE 403 570 521 77 17 tfor:TF2832|3end_possible_proton/sodium:glutamate_symporter_protein_3023939..3024089_POSITIVE 395 504 462 42 1 tfor:TF0677|3end_ATP-binding_protein,_Mrp/Nbp35_family,_involved_in_chromosome_partitioning_720982..721132_POSITIVE 389 526 488 44 7 tfor:TF2047|3end_hypothetical_protein_2210298..2210448_POSITIVE 389 495 455 99 57 tfor:TF0340|3end_hypothetical_protein_364257..364407_POSITIVE 387 505 461 49 6 tfor:TF2169|3end_hypothetical_protein_2345354..2345504_POSITIVE 385 505 461 38 4 tfor:TF0361|3end_hypothetical_protein_379810..379960_POSITIVE 383 475 436 131 94 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 382 504 461 71 38 tfor:TF2922|3end_surface_antigen_BspA_3121538..3121688_POSITIVE 380 350 302 119 69 tfor:TF3003|3end_hypothetical_protein_3220629..3220779_POSITIVE 375 590 547 42 2 tfor:TF1657|3end_lauroyl/myristoyl_acyltransferase_1773407..1773557_POSITIVE