Origin IGS: tagtaagaaaaaagagacggacaaatatcctttttttttaggtagaaaaagaaaaaatattttttttatgctgtaaaacatttttttctttttacctttgtcgcgcaaataaaaaacatcaatcaggattttaaatttttaaagag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctctttaaaaatttaaaatcctgattgatgttttttatttgcgcgacaaaggtaaaaagaaaaaaatgttttacagcataaaaaaaatattttttctttttctacctaaaaaaaaaggatatttgtccgtctcttttttcttacta Mask Tandem Repeat Region ================================================ tagtaagaaaaaagagacggacaaatatcctttttttttaggtagaaaaagaaaaaatattttttttatgctgtaaaacatttttttctttttacctttgtcgcgcaaataaaaaacatcaatcaggattttaaatttttaaagag Find is-nt database================================================ Query_seq: TF1525:TF1527|TF1525:TF1527:conserved hypothetical protein:hypothetical protein:->->:1632593..1632738 146 tagtaagaaaaaagagacggacaaatatcctttttttttaggtagaaaaagaaaaaatattttttttatgctgtaaaacatttttttctttttacctttgtcgcgcaaataaaaaacatcaatcaggattttaaatttttaaagag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1525:TF1527|TF1525:TF1527:conserved hypothetical protein:hypothetical protein:->->:1632593..1632738 146 tagtaagaaaaaagagacggacaaatatcctttttttttaggtagaaaaagaaaaaatattttttttatgctgtaaaacatttttttctttttacctttgtcgcgcaaataaaaaacatcaatcaggattttaaatttttaaagag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1525:TF1527|TF1525:TF1527:conserved hypothetical protein:hypothetical protein:->->:1632593..1632738 146 tagtaagaaaaaagagacggacaaatatcctttttttttaggtagaaaaagaaaaaatattttttttatgctgtaaaacatttttttctttttacctttgtcgcgcaaataaaaaacatcaatcaggattttaaatttttaaagag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagtaagaaaaaagagacggacaaatatcctttttttttaggtagaaaaagaaaaaatattttttttatgctgtaaaacatttttttctttttacctttgtcgcgcaaataaaaaacatcaatcaggattttaaatttttaaagag Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: - Start: 1 End: 129 L L F F S V S L Y G K K K P L F L F F I K K I S Y F M K K K K G K D R L Y F V D I L M ................. tagtaagaaaaaagagacggacaaatatcctttttttttaggtagaaaaagaaaaaatattttttttatgctgtaaaacatttttttctttttacctttgtcgcgcaaataaaaaacatcaatcaggattttaaatttttaaagag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagtaagaaaaaagagacggacaaatatcctttttttttaggtagaaaaagaaaaaatattttttttatgctgtaaaacatttttttctttttacctttgtcgcgcaaataaaaaacatcaatcaggattttaaatttttaaagag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1525:TF1527|TF1525:TF1527:conserved hypothetical protein:hypothetical protein:->->:1632593..1632738 146 tagtaagaaaaaagagacggacaaatatcctttttttttaggtagaaaaagaaaaaatattttttttatgctgtaaaacatttttttctttttacctttgtcgcgcaaataaaaaacatcaatcaggattttaaatttttaaagag Intra-Species Hit: Count: 1 Min: 1 Max: 146 Len: 146 Subject: tfor_TF1525_TF1527|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1632593,1632738]|146 HSP 1 e-value: 4.0E-22 bit: 105.0 Len: 53 Query Start:94 Query End:146 Subject Strand: POSITIVE Subject Start: 94 Subject End: 146 .............................................................................................acctttgtcgcgcaaataaaaaacatcaatcaggattttaaatttttaaagag .............................................................................................acctttgtcgcgcaaataaaaaacatcaatcaggattttaaatttttaaagag HSP 2 e-value: 2.0E-8 bit: 60.0 Len: 30 Query Start:1 Query End:30 Subject Strand: POSITIVE Subject Start: 1 Subject End: 30 tagtaagaaaaaagagacggacaaatatcc.................................................................................................................... tagtaagaaaaaagagacggacaaatatcc.................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUAAGAAAAAAGAGACGGACAAAUAUCCUUUUUUUUUAGGUAGAAAAAGAAAAAAUAUUUUUUUUAUGCUGUAAAACAUUUUUUUCUUUUUACCUUUGUCGCGCAAAUAAAAAACAUCAAUCAGGAUUUUAAAUUUUUAAAGAG ..((..((.((((((((.(((......))).)))))))).(((((((..((((((((......((((((...))))))...)))))))))))))))....))..))...(((((..(........)..)))))............. (-18.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUCUUUAAAAAUUUAAAAUCCUGAUUGAUGUUUUUUAUUUGCGCGACAAAGGUAAAAAGAAAAAAAUGUUUUACAGCAUAAAAAAAAUAUUUUUUCUUUUUCUACCUAAAAAAAAAGGAUAUUUGUCCGUCUCUUUUUUCUUACUA .............(((((..(........)..)))))...(((.((((((...((((((((((((.((((((...........))))))))))))))))))...(((........)))...)))))))))................ (-20.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 276 120 71 92 40 tfor:TF1467|5end_conserved_hypothetical_protein_1557011..1557241_POSITIVE 259 128 82 109 55 tfor:TF1522|5end_hypothetical_protein_1629531..1629761_POSITIVE 251 106 82 33 11 tfor:TF2229|5end_CTn_excision_protein;_DNA_topoisomerase_IA_2397536..2397766_POSITIVE 245 108 61 105 55 tfor:TF1021|5end_hypothetical_protein_1077513..1077743_POSITIVE 236 110 91 39 18 tfor:TF0219|5end_threonyl-tRNA_synthetase_243843..244073_POSITIVE 230 130 89 230 182 tfor:TF2523|5end_NAD(P)H-flavin_oxidoreductase_2702114..2702344_POSITIVE 223 107 79 117 79 tfor:TF1381|5end_hypothetical_protein_1456558..1456788_POSITIVE 215 120 77 82 32 tfor:TF2533|5end_adenylosuccinate_synthetase_2715666..2715896_POSITIVE 212 105 81 143 118 tfor:TF0573|5end_DNA_polymerase_III,_delta_subunit_603750..603980_POSITIVE 211 128 94 110 73 tfor:TF2216|5end_fumarate_hydratase,_class_I_2388227..2388457_POSITIVE 211 106 71 127 89 tfor:TF0612|5end_conserved_hypothetical_protein_644066..644296_POSITIVE 210 105 81 49 21 tfor:TF1899|5end_anti-sigma_factor_2052767..2052997_POSITIVE 209 105 61 153 114 tfor:TF2578|5end_30S_ribosomal_protein_S11_2746698..2746928_POSITIVE 209 107 63 158 123 tfor:TF2504|5end_peptidyl-prolyl_cis-trans_isomerase_2680031..2680261_POSITIVE 208 127 89 185 131 tfor:TF0322|5end_possible_YngK_protein_348345..348575_POSITIVE 207 105 71 208 173 tfor:TF1799|5end_quinolinate_synthetase_1940276..1940506_POSITIVE 207 42 5 149 102 tfor:TF0844|5end_transposase,_IS3_family_895204..895434_POSITIVE 203 107 70 142 96 tfor:TF2473|5end_poly(A)_polymerase/tRNA_nucleotidyltransferase_2650470..2650700_POSITIVE 203 106 71 50 6 tfor:TF1780|5end_formiminotransferase-cyclodeaminases_1921114..1921344_POSITIVE 196 30 2 130 100 tfor:TF0381|5end_cell_cycle_protein_401632..401862_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 276 120 71 85 33 tfor:TF1466|3end_DNA_gyrase_modulator_1557084..1557234_POSITIVE 259 128 82 93 39 tfor:TF1521|3end_GTP-binding_protein_1629595..1629745_POSITIVE 234 43 2 80 34 tfor:TF2484|3end_hypothetical_protein_2660306..2660456_POSITIVE 223 107 79 149 111 tfor:TF1379|3end_conserved_hypothetical_protein_1456670..1456820_POSITIVE 219 120 85 44 1 tfor:TF2532|3end_ferric-uptake_regulator_2715708..2715858_POSITIVE 216 114 89 142 117 tfor:TF2522|3end_deoxyuridine_5'-triphosphate_nucleotidohydrolase_(dUTPase)_2702129..2702279_POSITIVE 216 45 8 57 27 tfor:TF0985|3end_fructose-bisphosphate_aldolase_1042973..1043123_POSITIVE 211 128 94 52 15 tfor:TF2215|3end_conserved_hypothetical_protein_2388249..2388399_POSITIVE 209 105 61 140 101 tfor:TF2577|3end_30S_ribosomal_protein_S13_2746765..2746915_POSITIVE 208 127 89 57 3 tfor:TF0321|3end_glycosyltransferase_348297..348447_POSITIVE 207 42 5 92 45 tfor:TF0843|3end_transposase_895227..895377_POSITIVE 203 107 70 60 14 tfor:TF2472|3end_GAF_domain-containing_protein_2650468..2650618_POSITIVE 201 118 93 77 52 tfor:TF2583|3end_possible_ABC_transporter,__permease_component_2751026..2751176_POSITIVE 197 132 95 99 57 tfor:TF1968|3end_conserved_hypothetical_protein_2115959..2116109_POSITIVE 196 30 2 51 21 tfor:TF0380|3end_conserved_hypothetical_protein_401633..401783_POSITIVE 195 109 70 64 21 tfor:TF2847|3end_hypothetical_protein_3034985..3035135_POSITIVE 194 106 68 75 39 tfor:TF1084|3end_transposase_1153788..1153938_POSITIVE 192 107 70 83 47 tfor:TF2911|3end_hypothetical_protein_3106640..3106790_POSITIVE 192 103 79 26 6 tfor:TF2879|3end_tRNA_(guanine-N-1)-methyltransferase_3069008..3069158_POSITIVE 192 108 61 112 44 tfor:TF2337|3end_conserved_hypothetical_protein_2498982..2499132_POSITIVE