Origin IGS: taacaaattttatttcccatcgcctctctcctcccccttcaaaagaaaaaaacgaaacgcaggtttgaaggaggaggaagggaggccgagacataaaaaccgtctcataagcaatcgactaaaaaaacgctgggctgctcacgttttttgttgtaactttgaagacgcataacggagacaagtatcattaaataaaacaaatattgt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 acaatatttgttttatttaatgatacttgtctccgttatgcgtcttcaaagttacaacaaaaaacgtgagcagcccagcgtttttttagtcgattgcttatgagacggtttttatgtctcggcctcccttcctcctccttcaaacctgcgtttcgtttttttcttttgaagggggaggagagaggcgatgggaaataaaatttgtta Mask Tandem Repeat Region ================================================ taacaaattttatttcccatcgcctctctcctcccccttcaaaagaaaaaaacgaaacgcaggtttgaaggaggaggaagggaggccgagacataaaaaccgtctcataagcaatcgactaaaaaaacgctgggctgctcacgttttttgttgtaactttgaagacgcataacggagacaagtatcattaaataaaacaaatattgt Find is-nt database================================================ Query_seq: TF1519:TF1520|TF1519:TF1520:hypothetical protein:transaldolase:->->:1628083..1628289 207 taacaaattttatttcccatcgcctctctcctcccccttcaaaagaaaaaaacgaaacgcaggtttgaaggaggaggaagggaggccgagacataaaaaccgtctcataagcaatcgactaaaaaaacgctgggctgctcacgttttttgttgtaactttgaagacgcataacggagacaagtatcattaaataaaacaaatattgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1519:TF1520|TF1519:TF1520:hypothetical protein:transaldolase:->->:1628083..1628289 207 taacaaattttatttcccatcgcctctctcctcccccttcaaaagaaaaaaacgaaacgcaggtttgaaggaggaggaagggaggccgagacataaaaaccgtctcataagcaatcgactaaaaaaacgctgggctgctcacgttttttgttgtaactttgaagacgcataacggagacaagtatcattaaataaaacaaatattgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1519:TF1520|TF1519:TF1520:hypothetical protein:transaldolase:->->:1628083..1628289 207 taacaaattttatttcccatcgcctctctcctcccccttcaaaagaaaaaaacgaaacgcaggtttgaaggaggaggaagggaggccgagacataaaaaccgtctcataagcaatcgactaaaaaaacgctgggctgctcacgttttttgttgtaactttgaagacgcataacggagacaagtatcattaaataaaacaaatattgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacaaattttatttcccatcgcctctctcctcccccttcaaaagaaaaaaacgaaacgcaggtttgaaggaggaggaagggaggccgagacataaaaaccgtctcataagcaatcgactaaaaaaacgctgggctgctcacgttttttgttgtaactttgaagacgcataacggagacaagtatcattaaataaaacaaatattgt Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 65 End: 169 ................................................................ M K E E E G R P R H K N R L I S N R L K K R W A A H V F C C N F E D A ...................................... Protein_Len: 31 Strand: - Start: 108 End: 200 ........................................................................................................... L C D V L F V S P Q E R K K N Y S Q L R M V S V L I M L Y F M ....... Protein_Len: 34 Strand: - Start: 13 End: 114 ............ K G D G R E E G K L L F F R F A P K F S S S P L G L C L F R R M L M ............................................................................................. Protein_Len: 38 Strand: - Start: 2 End: 115 . V F K I E W R R E G G G E F S F F S V C T Q L L L F P P R S M F V T E Y A M ............................................................................................ taacaaattttatttcccatcgcctctctcctcccccttcaaaagaaaaaaacgaaacgcaggtttgaaggaggaggaagggaggccgagacataaaaaccgtctcataagcaatcgactaaaaaaacgctgggctgctcacgttttttgttgtaactttgaagacgcataacggagacaagtatcattaaataaaacaaatattgt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacaaattttatttcccatcgcctctctcctcccccttcaaaagaaaaaaacgaaacgcaggtttgaaggaggaggaagggaggccgagacataaaaaccgtctcataagcaatcgactaaaaaaacgctgggctgctcacgttttttgttgtaactttgaagacgcataacggagacaagtatcattaaataaaacaaatattgt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1519:TF1520|TF1519:TF1520:hypothetical protein:transaldolase:->->:1628083..1628289 207 taacaaattttatttcccatcgcctctctcctcccccttcaaaagaaaaaaacgaaacgcaggtttgaaggaggaggaagggaggccgagacataaaaaccgtctcataagcaatcgactaaaaaaacgctgggctgctcacgttttttgttgtaactttgaagacgcataacggagacaagtatcattaaataaaacaaatattgt Intra-Species Hit: Count: 1 Min: 1 Max: 207 Len: 207 Subject: tfor_TF1519_TF1520|hypothetical protein:transaldolase|POSITIVE:POSITIVE|[1628083, 1628289]|207 HSP 1 e-value: 8.0E-89 bit: 327.0 Len: 207 Query Start:1 Query End:207 Subject Strand: POSITIVE Subject Start: 1 Subject End: 207 taacaaattttatttcccatcgcctctctcctcccccttcaaaagnnnnnnncgaaacgcaggtttgaaggaggaggaagggaggccgagacataaaaaccgtctcataagcaatcgactnnnnnnncgctgggctgctcacgttttttgttgtaactttgaagacgcataacggagacaagtatcattaaataaaacaaatattgt taacaaattttatttcccatcgcctctctcctcccccttcaaaagaaaaaaacgaaacgcaggtttgaaggaggaggaagggaggccgagacataaaaaccgtctcataagcaatcgactaaaaaaacgctgggctgctcacgttttttgttgtaactttgaagacgcataacggagacaagtatcattaaataaaacaaatattgt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACAAAUUUUAUUUCCCAUCGCCUCUCUCCUCCCCCUUCAAAAGAAAAAAACGAAACGCAGGUUUGAAGGAGGAGGAAGGGAGGCCGAGACAUAAAAACCGUCUCAUAAGCAAUCGACUAAAAAAACGCUGGGCUGCUCACGUUUUUUGUUGUAACUUUGAAGACGCAUAACGGAGACAAGUAUCAUUAAAUAAAACAAAUAUUGU .......((((((((......(((((((((((((.((((((((....................)))))))).))))).)))))))).((.((........(((((((.(((....((((..((((((((.((((...))))))))))))))))...))))).)))))......(....)..)).))...)))))))).......... (-56.85) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACAAUAUUUGUUUUAUUUAAUGAUACUUGUCUCCGUUAUGCGUCUUCAAAGUUACAACAAAAAACGUGAGCAGCCCAGCGUUUUUUUAGUCGAUUGCUUAUGAGACGGUUUUUAUGUCUCGGCCUCCCUUCCUCCUCCUUCAAACCUGCGUUUCGUUUUUUUCUUUUGAAGGGGGAGGAGAGAGGCGAUGGGAAAUAAAAUUUGUUA ..((((...(((((((((...(((....))).(((((...((((((..((((..(.(((((((((((..........)))))))))..)).)...))))..))))))..............(((((..((((((((((((((((...(((...)))........)))))))))))))))).)))))))))).))))))))).)))). (-57.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 405 63 25 212 170 tfor:TF0656|5end_acetyltransferase,_possible_GNAT_family_702045..702275_POSITIVE 391 43 20 29 4 tfor:TF2746|5end_hypothetical_protein_2927996..2928226_POSITIVE 379 64 21 66 13 tfor:TF0107|5end_ion_channel_protein_113913..114143_POSITIVE 358 92 53 148 98 tfor:TF1339|5end_aspartate_1-decarboxylase_1420908..1421138_POSITIVE 357 88 58 74 37 tfor:TF0680|5end_tail-specific_protease_721393..721623_POSITIVE 333 117 73 56 8 tfor:TF2878|5end_conserved_hypothetical_protein;_possible_CAAX_amino_terminal_protease_family_3067304..3067534_POSITIVE 332 91 51 145 99 tfor:TF1804|5end_hypothetical_protein_1947101..1947331_POSITIVE 329 86 59 40 12 tfor:TF2940|5end_50S_ribosomal_protein_L11_3141367..3141597_POSITIVE 324 93 53 153 111 tfor:TF2747|5end_heat_shock_protein,_HSP90_family_2928279..2928509_POSITIVE 321 88 52 109 65 tfor:TF1029|5end_conserved_hypothetical_protein;_possible_phytoene_dehydrogenase_1086722..1086952_POSITIVE 321 94 52 61 15 tfor:TF0554|5end_Bacteroides_aerotolerance_protein,_BatC_586303..586533_POSITIVE 320 170 128 91 28 tfor:TF2853|5end_2-C-methyl-D-erythritol_2,4-cyclodiphosphate_synthase_3040172..3040402_POSITIVE 318 94 53 159 104 tfor:TF0748|5end_ATP-dependent_protease_La_792890..793120_POSITIVE 317 39 7 230 193 tfor:TF0830|5end_hypothetical_protein_887610..887840_POSITIVE 316 96 51 94 50 tfor:TF0015|5end_outer_membrane_protein_(possible_immunogenic_lipoprotein)_9555..9785_POSITIVE 315 103 62 176 137 tfor:TF2454|5end_D-alanyl-D-alanine_dipeptidase_2631259..2631489_POSITIVE 313 89 51 42 2 tfor:TF1305|5end_UDP-glucose_4-epimerase_1385623..1385853_POSITIVE 313 96 57 94 59 tfor:TF0992|5end_hypothetical_protein_1048251..1048481_POSITIVE 312 106 62 152 109 tfor:TF2422|5end_hypothetical_protein_2601298..2601528_POSITIVE 309 109 70 143 108 tfor:TF1423|5end_cell_division_protein_(ftsW)_1505942..1506172_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 389 75 31 41 3 tfor:TF1590|3end_hypothetical_protein_1706043..1706193_POSITIVE 359 70 23 61 21 tfor:TF1592|3end_hypothetical_protein_1708557..1708707_POSITIVE 358 92 53 75 25 tfor:TF1337|3end_multidrug_efflux_pump,_BexA_1420915..1421065_POSITIVE 357 88 58 90 53 tfor:TF0679|3end_hypothetical_protein_721489..721639_POSITIVE 332 91 51 84 38 tfor:TF1803|3end_conserved_hypothetical_protein_1947120..1947270_POSITIVE 322 120 76 151 97 tfor:TF0012|3end_conserved_hypothetical_protein_6641..6791_POSITIVE 321 88 52 82 38 tfor:TF1028|3end_1-acyl-sn-glycerol-3-phosphate_acyltransferase_1086775..1086925_POSITIVE 321 94 52 64 18 tfor:TF0553|3end_Bacteroides_aerotolerance_protein,_BatD_586386..586536_POSITIVE 318 93 58 40 2 tfor:TF2746|3end_hypothetical_protein_2928246..2928396_POSITIVE 318 94 53 99 44 tfor:TF0746|3end_possible_DNA_polymerase_III_792910..793060_POSITIVE 317 39 7 48 11 tfor:TF0828|3end_hypothetical_protein_887508..887658_POSITIVE 313 96 57 97 62 tfor:TF0991|3end_probable_NAD(FAD)-dependent_dehydrogenase_1048334..1048484_POSITIVE 307 168 125 148 97 tfor:TF0664|3end_small_protein_B_(SMPB)_709570..709720_POSITIVE 304 98 64 83 48 tfor:TF1519|3end_hypothetical_protein_1628022..1628172_POSITIVE 304 88 44 98 41 tfor:TF0844|3end_transposase,_IS3_family_896099..896249_POSITIVE 300 94 58 43 14 tfor:TF1775|3end_oxidoreductase,_Gfo/Idh/MocA_family_1914618..1914768_POSITIVE 300 78 33 62 23 tfor:TF0470|3end_hypothetical_protein_487518..487668_POSITIVE 297 43 18 33 9 tfor:TF1169|3end_lipoprotein_ABC_transporter,_permease_component_1251573..1251723_POSITIVE 296 37 9 119 87 tfor:TF2158|3end_conserved_hypothetical_protein_2334584..2334734_POSITIVE 294 102 66 59 12 tfor:TF2656|3end_valyl-tRNA_synthetase_2829822..2829972_POSITIVE