Origin IGS: taatgtgtcttactggttaaaaagcacattggcattaccattcggcatcaatgagcaataaaaagttgtttagaacattataaaaataattaaatcagagttttgcgaaatgtaggtataagattttttcttacctttggttcccctaacaaacaaatttattaaacg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cgtttaataaatttgtttgttaggggaaccaaaggtaagaaaaaatcttatacctacatttcgcaaaactctgatttaattatttttataatgttctaaacaactttttattgctcattgatgccgaatggtaatgccaatgtgctttttaaccagtaagacacatta Mask Tandem Repeat Region ================================================ taatgtgtcttactggttaaaaagcacattggcattaccattcggcatcaatgagcaataaaaagttgtttagaacattataaaaataattaaatcagagttttgcgaaatgtaggtataagattttttcttacctttggttcccctaacaaacaaatttattaaacg Find is-nt database================================================ Query_seq: TF1517:TF1518|TF1517:TF1518:hypothetical protein:peroxiredoxin:->->:1627008..1627175 168 taatgtgtcttactggttaaaaagcacattggcattaccattcggcatcaatgagcaataaaaagttgtttagaacattataaaaataattaaatcagagttttgcgaaatgtaggtataagattttttcttacctttggttcccctaacaaacaaatttattaaacg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1517:TF1518|TF1517:TF1518:hypothetical protein:peroxiredoxin:->->:1627008..1627175 168 taatgtgtcttactggttaaaaagcacattggcattaccattcggcatcaatgagcaataaaaagttgtttagaacattataaaaataattaaatcagagttttgcgaaatgtaggtataagattttttcttacctttggttcccctaacaaacaaatttattaaacg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1517:TF1518|TF1517:TF1518:hypothetical protein:peroxiredoxin:->->:1627008..1627175 168 taatgtgtcttactggttaaaaagcacattggcattaccattcggcatcaatgagcaataaaaagttgtttagaacattataaaaataattaaatcagagttttgcgaaatgtaggtataagattttttcttacctttggttcccctaacaaacaaatttattaaacg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatgtgtcttactggttaaaaagcacattggcattaccattcggcatcaatgagcaataaaaagttgtttagaacattataaaaataattaaatcagagttttgcgaaatgtaggtataagattttttcttacctttggttcccctaacaaacaaatttattaaacg Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: - Start: 73 End: 162 ........................................................................ F M I F I I L D S N Q S I Y T Y S K K K G K T G R V F L N M ...... taatgtgtcttactggttaaaaagcacattggcattaccattcggcatcaatgagcaataaaaagttgtttagaacattataaaaataattaaatcagagttttgcgaaatgtaggtataagattttttcttacctttggttcccctaacaaacaaatttattaaacg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatgtgtcttactggttaaaaagcacattggcattaccattcggcatcaatgagcaataaaaagttgtttagaacattataaaaataattaaatcagagttttgcgaaatgtaggtataagattttttcttacctttggttcccctaacaaacaaatttattaaacg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1517:TF1518|TF1517:TF1518:hypothetical protein:peroxiredoxin:->->:1627008..1627175 168 taatgtgtcttactggttaaaaagcacattggcattaccattcggcatcaatgagcaataaaaagttgtttagaacattataaaaataattaaatcagagttttgcgaaatgtaggtataagattttttcttacctttggttcccctaacaaacaaatttattaaacg Intra-Species Hit: Count: 1 Min: 1 Max: 168 Len: 168 Subject: tfor_TF1517_TF1518|hypothetical protein:peroxiredoxin|POSITIVE:POSITIVE|[1627008, 1627175]|168 HSP 1 e-value: 1.0E-90 bit: 333.0 Len: 168 Query Start:1 Query End:168 Subject Strand: POSITIVE Subject Start: 1 Subject End: 168 taatgtgtcttactggttaaaaagcacattggcattaccattcggcatcaatgagcaataaaaagttgtttagaacattataaaaataattaaatcagagttttgcgaaatgtaggtataagattttttcttacctttggttcccctaacaaacaaatttattaaacg taatgtgtcttactggttaaaaagcacattggcattaccattcggcatcaatgagcaataaaaagttgtttagaacattataaaaataattaaatcagagttttgcgaaatgtaggtataagattttttcttacctttggttcccctaacaaacaaatttattaaacg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGUGUCUUACUGGUUAAAAAGCACAUUGGCAUUACCAUUCGGCAUCAAUGAGCAAUAAAAAGUUGUUUAGAACAUUAUAAAAAUAAUUAAAUCAGAGUUUUGCGAAAUGUAGGUAUAAGAUUUUUUCUUACCUUUGGUUCCCCUAACAAACAAAUUUAUUAAACG ...(((......((((((.....((....(((.....)))....)).....((((((((.....))))))))....................)))))).(((..(.(((....(((((..(((.....))))))))....))).)..)))..)))............. (-26.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGUUUAAUAAAUUUGUUUGUUAGGGGAACCAAAGGUAAGAAAAAAUCUUAUACCUACAUUUCGCAAAACUCUGAUUUAAUUAUUUUUAUAAUGUUCUAAACAACUUUUUAUUGCUCAUUGAUGCCGAAUGGUAAUGCCAAUGUGCUUUUUAACCAGUAAGACACAUUA .......((((((..(((((..((....))..((((((((.....)))..))))).......))))).....))))))..........(((((((((.....(((..(((..((.(((((.((((....))))....))))).))....)))..))).))).)))))) (-26.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 279 56 14 125 88 tfor:TF0700|5end_possible_lipoprotein_742649..742879_POSITIVE 266 59 24 225 190 tfor:TF3154|5end_conserved_hypothetical_protein_3389219..3389449_POSITIVE 261 56 12 184 130 tfor:TF1040|5end_dihydroorotate_dehydrogenase,_electron_transfer_subunit_1101253..1101483_POSITIVE 259 55 13 61 12 tfor:TF2014|5end_dipeptidyl_aminopeptidase_IV_2169398..2169628_POSITIVE 259 60 13 51 11 tfor:TF0890|5end_transfer_region-related_protein,_TraQ_940014..940244_POSITIVE 255 55 23 207 185 tfor:TF1448|5end_possible_competence_protein_1531711..1531941_POSITIVE 255 56 14 165 119 tfor:TF0344|5end_hypothetical_protein_366722..366952_POSITIVE 247 56 15 181 138 tfor:TF2177|5end_hypothetical_protein_2349281..2349511_POSITIVE 246 50 8 161 121 tfor:TF2737|5end_conserved_hypothetical_protein;_TPR-repeat-containing_protein_2918397..2918627_POSITIVE 244 66 29 186 131 tfor:TF3098|5end_conserved_hypothetical_protein_3329913..3330143_POSITIVE 244 64 24 198 152 tfor:TF2661|5end_hypothetical_protein_2833509..2833739_POSITIVE 244 144 103 67 24 tfor:TF1915|5end_NAD-dependent_protein_deacetylase,_SIR2_family_2067720..2067950_POSITIVE 244 48 7 69 28 tfor:TF1064|5end_ampG_permease_1134004..1134234_POSITIVE 243 65 22 88 41 tfor:TF2139|5end_hypothetical_protein_2315661..2315891_POSITIVE 243 57 12 231 198 tfor:TF1651|5end_integrase_1761296..1761526_POSITIVE 240 56 22 191 150 tfor:TF2673|5end_conserved_hypothetical_protein_2846987..2847217_POSITIVE 240 45 10 44 12 tfor:TF2205|5end_conserved_hypothetical_protein;_possible_transcriptional_regulator_2379055..2379285_POSITIVE 239 53 24 114 80 tfor:TF2657|5end_conserved_hypothetical_protein_2829729..2829959_POSITIVE 238 60 24 50 7 tfor:TF1033|5end_endothelin_converting_enzyme,_endopeptidase_1092772..1093002_POSITIVE 235 45 3 166 136 tfor:TF0825|5end_hypothetical_protein_883597..883827_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 279 56 14 141 104 tfor:TF0699|3end_pyridoxal_phosphate_biosynthetic_protein_742745..742895_POSITIVE 272 46 3 51 10 tfor:TF3034|3end_conserved_hypothetical_protein_3260434..3260584_POSITIVE 263 72 31 90 47 tfor:TF2340|3end_hypothetical_protein_2500915..2501065_POSITIVE 250 57 13 96 37 tfor:TF2911|3end_hypothetical_protein_3106640..3106790_POSITIVE 246 50 8 142 102 tfor:TF2736|3end_methylglyoxal_synthase_2918458..2918608_POSITIVE 245 54 12 149 99 tfor:TF1039|3end_conserved_hypothetical_protein_1101302..1101452_POSITIVE 244 64 24 149 103 tfor:TF2660|3end_hypothetical_protein_2833540..2833690_POSITIVE 244 48 7 49 8 tfor:TF1063|3end_possible_amidase_enhancer_precursor;_sporulation-related_protein_1134064..1134214_POSITIVE 240 56 22 138 97 tfor:TF2672|3end_lipoic_acid_synthetase_2847014..2847164_POSITIVE 239 53 24 127 93 tfor:TF2656|3end_valyl-tRNA_synthetase_2829822..2829972_POSITIVE 231 72 31 94 49 tfor:TF0959|3end_periplasmic_protease_1008540..1008690_POSITIVE 229 58 23 142 102 tfor:TF0785|3end_biopolymer_transport_protein,_MotA/TolQ/ExbB_proton_channel_family_838600..838750_POSITIVE 226 49 1 131 87 tfor:TF2677|3end_aspartate_transaminase_2852079..2852229_POSITIVE 226 58 13 62 18 tfor:TF2515|3end_outer_membrane_protein,_TonB_dependent_receptor_2694865..2695015_POSITIVE 226 56 13 136 84 tfor:TF0599|3end_hypothetical_protein_630130..630280_POSITIVE 225 49 4 145 99 tfor:TF0952|3end_conserved_hypothetical_protein_998009..998159_POSITIVE 224 48 3 94 50 tfor:TF2903|3end_translation_initiation_factor_SUI1_3098986..3099136_POSITIVE 223 60 11 118 70 tfor:TF2089|3end_acyl_carrier_protein_2259749..2259899_POSITIVE 222 69 23 150 110 tfor:TF1974|3end_glyoxalase_I_related_protein_(lactoylglutathione_lyase)_2121428..2121578_POSITIVE 221 46 7 46 6 tfor:TF1952|3end_immunoreactive_84kD_antigen_PG93_2103829..2103979_POSITIVE