Origin IGS: tttctactttttacctacttgacgcttgatccggcaaacaccctatgataaatttgtttttttaaacatctccttagaaatcgtttacatttcagcatttccgaattgccttcgccttcttaccgttaagtctgataaaaagcaaatacggaatcaagcattta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaatgcttgattccgtatttgctttttatcagacttaacggtaagaaggcgaaggcaattcggaaatgctgaaatgtaaacgatttctaaggagatgtttaaaaaaacaaatttatcatagggtgtttgccggatcaagcgtcaagtaggtaaaaagtagaaa Mask Tandem Repeat Region ================================================ tttctactttttacctacttgacgcttgatccggcaaacaccctatgataaatttgtttttttaaacatctccttagaaatcgtttacatttcagcatttccgaattgccttcgccttcttaccgttaagtctgataaaaagcaaatacggaatcaagcattta Find is-nt database================================================ Query_seq: TF1508:TF1509|TF1508:TF1509:anti-sigma factor:possible acylaminoacyl-peptidase:->->:1617514..1617677 164 tttctactttttacctacttgacgcttgatccggcaaacaccctatgataaatttgtttttttaaacatctccttagaaatcgtttacatttcagcatttccgaattgccttcgccttcttaccgttaagtctgataaaaagcaaatacggaatcaagcattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1508:TF1509|TF1508:TF1509:anti-sigma factor:possible acylaminoacyl-peptidase:->->:1617514..1617677 164 tttctactttttacctacttgacgcttgatccggcaaacaccctatgataaatttgtttttttaaacatctccttagaaatcgtttacatttcagcatttccgaattgccttcgccttcttaccgttaagtctgataaaaagcaaatacggaatcaagcattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1508:TF1509|TF1508:TF1509:anti-sigma factor:possible acylaminoacyl-peptidase:->->:1617514..1617677 164 tttctactttttacctacttgacgcttgatccggcaaacaccctatgataaatttgtttttttaaacatctccttagaaatcgtttacatttcagcatttccgaattgccttcgccttcttaccgttaagtctgataaaaagcaaatacggaatcaagcattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttctactttttacctacttgacgcttgatccggcaaacaccctatgataaatttgtttttttaaacatctccttagaaatcgtttacatttcagcatttccgaattgccttcgccttcttaccgttaagtctgataaaaagcaaatacggaatcaagcattta Predict ORF larger than 30AA ================================================ Protein_Len: 46 Strand: + Start: 26 End: 163 ......................... M I R Q T P Y D K F V F L N I S L E I V Y I S A F P N C L R L L T V K S D K K Q I R N Q A F . tttctactttttacctacttgacgcttgatccggcaaacaccctatgataaatttgtttttttaaacatctccttagaaatcgtttacatttcagcatttccgaattgccttcgccttcttaccgttaagtctgataaaaagcaaatacggaatcaagcattta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttctactttttacctacttgacgcttgatccggcaaacaccctatgataaatttgtttttttaaacatctccttagaaatcgtttacatttcagcatttccgaattgccttcgccttcttaccgttaagtctgataaaaagcaaatacggaatcaagcattta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1508:TF1509|TF1508:TF1509:anti-sigma factor:possible acylaminoacyl-peptidase:->->:1617514..1617677 164 tttctactttttacctacttgacgcttgatccggcaaacaccctatgataaatttgtttttttaaacatctccttagaaatcgtttacatttcagcatttccgaattgccttcgccttcttaccgttaagtctgataaaaagcaaatacggaatcaagcattta Intra-Species Hit: Count: 1 Min: 1 Max: 164 Len: 164 Subject: tfor_TF1508_TF1509|anti-sigma factor:possible acylaminoacyl-peptidase|POSITIVE:POSITIVE|[1617514, 1617677]|164 HSP 1 e-value: 9.0E-76 bit: 283.0 Len: 164 Query Start:1 Query End:164 Subject Strand: POSITIVE Subject Start: 1 Subject End: 164 tttctactttttacctacttgacgcttgatccggcaaacaccctatgataaatttgnnnnnnnaaacatctccttagaaatcgtttacatttcagcatttccgaattgccttcgccttcttaccgttaagtctgataaaaagcaaatacggaatcaagcattta tttctactttttacctacttgacgcttgatccggcaaacaccctatgataaatttgtttttttaaacatctccttagaaatcgtttacatttcagcatttccgaattgccttcgccttcttaccgttaagtctgataaaaagcaaatacggaatcaagcattta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUCUACUUUUUACCUACUUGACGCUUGAUCCGGCAAACACCCUAUGAUAAAUUUGUUUUUUUAAACAUCUCCUUAGAAAUCGUUUACAUUUCAGCAUUUCCGAAUUGCCUUCGCCUUCUUACCGUUAAGUCUGAUAAAAAGCAAAUACGGAAUCAAGCAUUUA .......................(((((((..(((((.................(((((....))))).....(..(((((.(((........))))))))..).)))))............((((.....((.......)).....)))).)))))))..... (-24.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUGCUUGAUUCCGUAUUUGCUUUUUAUCAGACUUAACGGUAAGAAGGCGAAGGCAAUUCGGAAAUGCUGAAAUGUAAACGAUUUCUAAGGAGAUGUUUAAAAAAACAAAUUUAUCAUAGGGUGUUUGCCGGAUCAAGCGUCAAGUAGGUAAAAAGUAGAAA ..................(((((((((((((..((((.(((.(.....((((((.((..(((((.....)))))...(((((.(((((....)))))))))).....................)).))))))......).))).)))).))))))))))))).. (-39.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 343 121 88 209 180 tfor:TF2498|5end_electron_transfer_flavoprotein,_alpha_subunit_2672801..2673031_POSITIVE 322 137 92 207 157 tfor:TF0302|5end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_328641..328871_POSITIVE 310 127 81 200 151 tfor:TF2810|5end_conserved_hypothetical_protein_3000583..3000813_POSITIVE 306 47 8 55 18 tfor:TF1068|5end_conserved_hypothetical_protein;_possible_N-acetylglucosamine_kinase_1138036..1138266_POSITIVE 295 148 101 196 152 tfor:TF0204|5end_cation_efflux_system_protein_230618..230848_POSITIVE 292 140 96 202 142 tfor:TF1489|5end_argininosuccinate_lyase_1588904..1589134_POSITIVE 287 127 84 61 14 tfor:TF0644|5end_pyruvate_phosphate_dikinase_685784..686014_POSITIVE 285 128 83 60 7 tfor:TF1538|5end_conserved_hypothetical_protein_1643531..1643761_POSITIVE 284 137 106 210 180 tfor:TF2531|5end_possible_dipeptidyl-peptidase_III_2713045..2713275_POSITIVE 281 126 95 129 90 tfor:TF0600|5end_oxidoreductase/dehydrogenase_630083..630313_POSITIVE 281 133 92 210 157 tfor:TF0136|5end_transposase_element_145627..145857_POSITIVE 281 133 92 125 72 tfor:TF0134|5end_transposase_element_145542..145772_POSITIVE 276 116 72 61 26 tfor:TF0831|5end_conserved_hypothetical_protein_888183..888413_POSITIVE 276 134 95 213 173 tfor:TF0277|5end_outer_membrane_protein_303936..304166_POSITIVE 273 133 91 191 147 tfor:TF2226|5end_CTn_excision_protein_2395529..2395759_POSITIVE 273 136 94 200 156 tfor:TF0289|5end_conserved_hypothetical_protein_318015..318245_POSITIVE 272 120 72 123 65 tfor:TF0292|5end_conserved_hypothetical_protein_320087..320317_POSITIVE 270 45 14 42 1 tfor:TF2909|5end_GTP-binding_protein,_possible_GTP1/OBG_family_3104644..3104874_POSITIVE 269 120 72 176 121 tfor:TF1840|5end_transposase_1990221..1990451_POSITIVE 267 133 95 153 108 tfor:TF2880|5end_phenylalanyl-tRNA_synthetase,_beta_subunit_3069052..3069282_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 307 127 83 48 2 tfor:TF2809|3end_conserved_hypothetical_protein;_possible_translation_factor_(SUA5)_3000511..3000661_POSITIVE 306 47 8 55 18 tfor:TF1067|3end_conserved_hypothetical_protein_1138116..1138266_POSITIVE 281 126 95 96 57 tfor:TF0599|3end_hypothetical_protein_630130..630280_POSITIVE 281 133 92 59 6 tfor:TF0133|3end_transposase_element_145556..145706_POSITIVE 276 116 72 48 13 tfor:TF0829|3end_conserved_hypothetical_protein_888250..888400_POSITIVE 272 120 72 87 29 tfor:TF0291|3end_triose-phosphate_isomerase_320131..320281_POSITIVE 270 134 93 61 23 tfor:TF0095|3end_methyltransferase_106845..106995_POSITIVE 264 127 87 42 7 tfor:TF2744|3end_methionyl-tRNA_formyltransferase_2927378..2927528_POSITIVE 263 138 96 139 93 tfor:TF1008|3end_rod_shape-determining_protein_MreC_1064374..1064524_POSITIVE 262 128 82 87 44 tfor:TF0810|3end_possible_outer_membrane_efflux_protein_869696..869846_POSITIVE 259 133 91 133 92 tfor:TF2937|3end_translation_elongation_factor_Tu_3140502..3140652_POSITIVE 257 132 93 121 71 tfor:TF3154|3end_conserved_hypothetical_protein_3390489..3390639_POSITIVE 256 46 4 75 27 tfor:TF3133|3end_conserved_hypothetical_protein_3369913..3370063_POSITIVE 254 121 93 30 1 tfor:TF0167|3end_conserved_hypothetical_protein_178234..178384_POSITIVE 253 127 81 85 45 tfor:TF3126|3end_glycosyltransferase,_group_2_family_3361685..3361835_POSITIVE 253 59 11 77 12 tfor:TF3118|3end_conserved_hypothetical_protein_3352824..3352974_POSITIVE 250 125 82 140 84 tfor:TF2685|3end_iron-regulated_ABC-type_transporter_membrane_component_2862725..2862875_POSITIVE 250 144 101 115 65 tfor:TF0901|3end_conserved_hypothetical_protein_947273..947423_POSITIVE 247 104 62 77 34 tfor:TF0120|3end_hexouronate_transporter_132811..132961_POSITIVE 246 125 83 47 6 tfor:TF0214|3end_DNA_recombination_and_repair_protein,_RecN_241571..241721_POSITIVE