Origin IGS: agcctataatctccaaaaaaaaacgatgcaaaggtatatgttttttgtatagaaaaagaatgattccttatatttttcagtaacaatcaaattataaccatcttacaaaaccataaactccgaaagaacagacttgtcttatttcattcatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatgaatgaaataagacaagtctgttctttcggagtttatggttttgtaagatggttataatttgattgttactgaaaaatataaggaatcattctttttctatacaaaaaacatatacctttgcatcgtttttttttggagattataggct Mask Tandem Repeat Region ================================================ agcctataatctccaaaaaaaaacgatgcaaaggtatatgttttttgtatagaaaaagaatgattccttatatttttcagtaacaatcaaattataaccatcttacaaaaccataaactccgaaagaacagacttgtcttatttcattcatta Find is-nt database================================================ Query_seq: TF1500:TF1501|TF1500:TF1501:conserved hypothetical protein:sugar hydrolase:->->:1600415..1600567 153 agcctataatctccaaaaaaaaacgatgcaaaggtatatgttttttgtatagaaaaagaatgattccttatatttttcagtaacaatcaaattataaccatcttacaaaaccataaactccgaaagaacagacttgtcttatttcattcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1500:TF1501|TF1500:TF1501:conserved hypothetical protein:sugar hydrolase:->->:1600415..1600567 153 agcctataatctccaaaaaaaaacgatgcaaaggtatatgttttttgtatagaaaaagaatgattccttatatttttcagtaacaatcaaattataaccatcttacaaaaccataaactccgaaagaacagacttgtcttatttcattcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1500:TF1501|TF1500:TF1501:conserved hypothetical protein:sugar hydrolase:->->:1600415..1600567 153 agcctataatctccaaaaaaaaacgatgcaaaggtatatgttttttgtatagaaaaagaatgattccttatatttttcagtaacaatcaaattataaccatcttacaaaaccataaactccgaaagaacagacttgtcttatttcattcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agcctataatctccaaaaaaaaacgatgcaaaggtatatgttttttgtatagaaaaagaatgattccttatatttttcagtaacaatcaaattataaccatcttacaaaaccataaactccgaaagaacagacttgtcttatttcattcatta Predict ORF larger than 30AA ================================================ agcctataatctccaaaaaaaaacgatgcaaaggtatatgttttttgtatagaaaaagaatgattccttatatttttcagtaacaatcaaattataaccatcttacaaaaccataaactccgaaagaacagacttgtcttatttcattcatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agcctataatctccaaaaaaaaacgatgcaaaggtatatgttttttgtatagaaaaagaatgattccttatatttttcagtaacaatcaaattataaccatcttacaaaaccataaactccgaaagaacagacttgtcttatttcattcatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1500:TF1501|TF1500:TF1501:conserved hypothetical protein:sugar hydrolase:->->:1600415..1600567 153 agcctataatctccaaaaaaaaacgatgcaaaggtatatgttttttgtatagaaaaagaatgattccttatatttttcagtaacaatcaaattataaccatcttacaaaaccataaactccgaaagaacagacttgtcttatttcattcatta Intra-Species Hit: Count: 1 Min: 24 Max: 153 Len: 130 Subject: tfor_TF1500_TF1501|conserved hypothetical protein:sugar hydrolase|POSITIVE:POSITIVE|[1600415,1600567]|153 HSP 1 e-value: 5.0E-68 bit: 258.0 Len: 130 Query Start:24 Query End:153 Subject Strand: POSITIVE Subject Start: 24 Subject End: 153 .......................cgatgcaaaggtatatgttttttgtatagaaaaagaatgattccttatatttttcagtaacaatcaaattataaccatcttacaaaaccataaactccgaaagaacagacttgtcttatttcattcatta .......................cgatgcaaaggtatatgttttttgtatagaaaaagaatgattccttatatttttcagtaacaatcaaattataaccatcttacaaaaccataaactccgaaagaacagacttgtcttatttcattcatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGAUGCAAAGGUAUAUGUUUUUUGUAUAGAAAAAGAAUGAUUCCUUAUAUUUUUCAGUAACAAUCAAAUUAUAACCAUCUUACAAAACCAUAAACUCCGAAAGAACAGACUUGUCUUAUUUCAUUCAUUA .((((....(((.((((..(((((((..((((((..((((....)))).)))))).((((........))))........))))))).)))).)))..((((....(((....)))..))))...)))). (-14.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGAAUGAAAUAAGACAAGUCUGUUCUUUCGGAGUUUAUGGUUUUGUAAGAUGGUUAUAAUUUGAUUGUUACUGAAAAAUAUAAGGAAUCAUUCUUUUUCUAUACAAAAAACAUAUACCUUUGCAUCG ...((((.(((...(((....))).))).))))..((.((((.(((((((.((..((((.....))))..)).............(((((........))))).)))))))..)))).)).......... (-18.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 247 57 14 110 65 tfor:TF2934|5end_acetyltransferase/carbonic_anhydrase_3136647..3136877_POSITIVE 230 115 81 104 66 tfor:TF1680|5end_peptide_chain_release_factor_3_1793315..1793545_POSITIVE 219 35 1 47 15 tfor:TF2373|5end_UDP-3-O-acyl-GlcNAc_deacetylase_2538344..2538574_POSITIVE 219 43 2 169 123 tfor:TF0461|5end_ATP:cob(I)alamin_adenosyltransferase_476990..477220_POSITIVE 218 40 2 146 101 tfor:TF1701|5end_transcriptional_regulator_1821493..1821723_POSITIVE 213 126 93 149 119 tfor:TF3110|5end_conserved_hypothetical_protein_with_helicase_domain_3342714..3342944_POSITIVE 210 46 1 69 28 tfor:TF2752|5end_endonuclease_III_2936781..2937011_POSITIVE 210 127 93 115 79 tfor:TF1003|5end_conserved_hypothetical_protein;_possible_secreted_glycosylhydrolase_1058177..1058407_POSITIVE 208 130 81 169 109 tfor:TF2286|5end_hypothetical_protein_2440901..2441131_POSITIVE 208 37 3 137 93 tfor:TF0426|5end_conserved_hypothetical_protein_444728..444958_POSITIVE 207 49 7 92 47 tfor:TF2142|5end_hypothetical_protein_2318416..2318646_POSITIVE 205 32 1 36 7 tfor:TF2845|5end_hypothetical_protein_3034414..3034644_POSITIVE 204 42 1 49 4 tfor:TF0233|5end_DNA_polymerase_III_gamma/tau_subunit_259355..259585_POSITIVE 197 114 75 49 6 tfor:TF2765|5end_subtilisin-like_serine_protease_2949635..2949865_POSITIVE 197 45 1 158 115 tfor:TF0967|5end_alpha_glycan_phosphorylase_1016231..1016461_POSITIVE 192 123 86 226 181 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 192 116 86 63 25 tfor:TF1333|5end_probable_acylaminoacyl_peptidase;_alanyl_dipeptidyl_peptidase_1413397..1413627_POSITIVE 192 123 86 181 136 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 189 117 75 143 88 tfor:TF0950|5end_ribosomal_large_subunit_pseudouridine_synthase_D_(pseudouridylate_synthase)_(uracil_hydrolyase)_994630..994860_POSITIVE 188 129 86 159 119 tfor:TF1601|5end_conserved_hypothetical_protein_1714731..1714961_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 247 57 14 110 65 tfor:TF2932|3end_capsule_biosynthesis_protein_3136727..3136877_POSITIVE 219 35 1 40 8 tfor:TF2372|3end_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosami_ne_acyltransferase_2538417..2538567_POSITIVE 210 127 93 90 54 tfor:TF1002|3end_replicative_DNA_helicase_1058232..1058382_POSITIVE 197 114 75 49 6 tfor:TF2764|3end_hypothetical_protein_2949715..2949865_POSITIVE 196 25 1 77 55 tfor:TF2515|3end_outer_membrane_protein,_TonB_dependent_receptor_2694865..2695015_POSITIVE 192 116 86 70 32 tfor:TF1332|3end_cytochrome_c-type_synthesis_protein_(cytochrome_c_biogenesis_protein);_ABC_transporter,_permease_1413484..1413634_POSITIVE 189 117 75 131 76 tfor:TF0949|3end_conserved_hypothetical_protein_994698..994848_POSITIVE 187 115 96 28 7 tfor:TF1679|3end_conserved_hypothetical_protein_1793319..1793469_POSITIVE 187 115 88 136 108 tfor:TF0444|3end_Na+-translocating_NADH-quinone_oxidoreductase,_subunit_5_466518..466668_POSITIVE 184 23 1 89 65 tfor:TF0805|3end_possible_hydrolase,_HAD_superfamily_863627..863777_POSITIVE 183 46 9 96 54 tfor:TF2682|3end_N_utilization_substance_protein_A_2857770..2857920_POSITIVE 183 107 61 44 2 tfor:TF1957|3end_penicillin_tolerance_protein;_possible_isoprene_biosynthesis_related_protein_2108732..2108882_POSITIVE 182 114 71 119 69 tfor:TF3146|3end_GTP-binding_protein_Obg_3383080..3383230_POSITIVE 182 99 71 140 113 tfor:TF2511|3end_RNA_binding_protein_2690232..2690382_POSITIVE 181 116 95 126 105 tfor:TF2791|3end_GTP-binding_protein_TypA_2977219..2977369_POSITIVE 181 52 16 54 21 tfor:TF1006|3end_bifunctional_purine_biosynthesis_protein,_phosphoribosylaminoimidazolecarboxamide_formyltransferase_and_IMP_cyclohydrolase_1062393..1062543_POSITIVE 179 37 2 123 87 tfor:TF0527|3end_prolyl_endopeptidase_551950..552100_POSITIVE 178 124 82 115 70 tfor:TF2613|3end_conserved_hypothetical_protein_2789798..2789948_POSITIVE 178 90 41 63 12 tfor:TF1638|3end_conserved_hypothetical_protein_1747732..1747882_POSITIVE 178 57 16 99 48 tfor:TF0589|3end_anti-sigma_factor_624603..624753_POSITIVE