Origin IGS: taatcgacaagccgaaggatcgatgaattttaccatccccccctgaaccgttcgcactattcctgtactatacatgcattgttctttcaaatatccgatacttctatcgcctgacgaccgggtgaataaaaacctttatgctttcttttt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaaaagaaagcataaaggtttttattcacccggtcgtcaggcgatagaagtatcggatatttgaaagaacaatgcatgtatagtacaggaatagtgcgaacggttcagggggggatggtaaaattcatcgatccttcggcttgtcgatta Mask Tandem Repeat Region ================================================ taatcgacaagccgaaggatcgatgaattttaccatccccccctgaaccgttcgcactattcctgtactatacatgcattgttctttcaaatatccgatacttctatcgcctgacgaccgggtgaataaaaacctttatgctttcttttt Find is-nt database================================================ Query_seq: TF1490:TF1491|TF1490:TF1491:hypothetical protein:mannosyltransferase:->->:1590843..1590992 150 taatcgacaagccgaaggatcgatgaattttaccatccccccctgaaccgttcgcactattcctgtactatacatgcattgttctttcaaatatccgatacttctatcgcctgacgaccgggtgaataaaaacctttatgctttcttttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1490:TF1491|TF1490:TF1491:hypothetical protein:mannosyltransferase:->->:1590843..1590992 150 taatcgacaagccgaaggatcgatgaattttaccatccccccctgaaccgttcgcactattcctgtactatacatgcattgttctttcaaatatccgatacttctatcgcctgacgaccgggtgaataaaaacctttatgctttcttttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1490:TF1491|TF1490:TF1491:hypothetical protein:mannosyltransferase:->->:1590843..1590992 150 taatcgacaagccgaaggatcgatgaattttaccatccccccctgaaccgttcgcactattcctgtactatacatgcattgttctttcaaatatccgatacttctatcgcctgacgaccgggtgaataaaaacctttatgctttcttttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatcgacaagccgaaggatcgatgaattttaccatccccccctgaaccgttcgcactattcctgtactatacatgcattgttctttcaaatatccgatacttctatcgcctgacgaccgggtgaataaaaacctttatgctttcttttt Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 19 End: 111 .................. M D E F Y H P P L N R S H Y S C T I H A L F F Q I S D T S I A ....................................... Protein_Len: 32 Strand: + Start: 27 End: 122 .......................... M L P S P P E P F A L F L Y Y T C I V L S N I R Y F Y R L T T G ............................ Protein_Len: 30 Strand: + Start: 59 End: 148 .......................................................... M P V L Y M H C S F K Y P I L L S P D D R V N K N L Y A F F .. Protein_Len: 33 Strand: - Start: 1 End: 99 L R C A S P D I F K V M G G Q V T R V I G T S Y M C Q E K L Y G M ................................................... taatcgacaagccgaaggatcgatgaattttaccatccccccctgaaccgttcgcactattcctgtactatacatgcattgttctttcaaatatccgatacttctatcgcctgacgaccgggtgaataaaaacctttatgctttcttttt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatcgacaagccgaaggatcgatgaattttaccatccccccctgaaccgttcgcactattcctgtactatacatgcattgttctttcaaatatccgatacttctatcgcctgacgaccgggtgaataaaaacctttatgctttcttttt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1490:TF1491|TF1490:TF1491:hypothetical protein:mannosyltransferase:->->:1590843..1590992 150 taatcgacaagccgaaggatcgatgaattttaccatccccccctgaaccgttcgcactattcctgtactatacatgcattgttctttcaaatatccgatacttctatcgcctgacgaccgggtgaataaaaacctttatgctttcttttt Intra-Species Hit: Count: 1 Min: 1 Max: 150 Len: 150 Subject: tfor_TF1490_TF1491|hypothetical protein:mannosyltransferase|POSITIVE:POSITIVE|[1590843, 1590992]|150 HSP 1 e-value: 2.0E-67 bit: 256.0 Len: 150 Query Start:1 Query End:150 Subject Strand: POSITIVE Subject Start: 1 Subject End: 150 taatcgacaagccgaaggatcgatgaattttaccatnnnnnnntgaaccgttcgcactattcctgtactatacatgcattgttctttcaaatatccgatacttctatcgcctgacgaccgggtgaataaaaacctttatgctttcttttt taatcgacaagccgaaggatcgatgaattttaccatccccccctgaaccgttcgcactattcctgtactatacatgcattgttctttcaaatatccgatacttctatcgcctgacgaccgggtgaataaaaacctttatgctttcttttt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCGACAAGCCGAAGGAUCGAUGAAUUUUACCAUCCCCCCCUGAACCGUUCGCACUAUUCCUGUACUAUACAUGCAUUGUUCUUUCAAAUAUCCGAUACUUCUAUCGCCUGACGACCGGGUGAAUAAAAACCUUUAUGCUUUCUUUUU .....((.((((.(((((...((((........)))).......((((.((..(((.......((((...))))))))).))))......................(((((((.....))))))).......)))))..))))))..... (-23.81) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAAGAAAGCAUAAAGGUUUUUAUUCACCCGGUCGUCAGGCGAUAGAAGUAUCGGAUAUUUGAAAGAACAAUGCAUGUAUAGUACAGGAAUAGUGCGAACGGUUCAGGGGGGGAUGGUAAAAUUCAUCGAUCCUUCGGCUUGUCGAUUA .....((((((......))))))........(((((.(((((........................((((..(((((................)))))....)))).(((((((((((......)))))..)))))).))))).))))). (-32.89) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 366 60 11 112 58 tfor:TF3070|5end_conserved_hypothetical_protein_3294380..3294610_POSITIVE 360 125 94 50 18 tfor:TF2337|5end_conserved_hypothetical_protein_2497997..2498227_POSITIVE 342 59 17 52 14 tfor:TF2837|5end_conserved_hypothetical_protein_3027939..3028169_POSITIVE 340 40 7 95 62 tfor:TF0255|5end_conserved_hypothetical_protein_285807..286037_POSITIVE 336 68 21 67 11 tfor:TF0814|5end_conserved_hypothetical_protein_872546..872776_POSITIVE 334 60 13 49 1 tfor:TF1824|5end_ABC_transporter,_ATP-binding_protein_1972537..1972767_POSITIVE 331 50 2 155 84 tfor:TF0352|5end_transposase_element_371223..371453_POSITIVE 327 66 34 223 186 tfor:TF2729|5end_possible_outer_membrane_protein_2908216..2908446_POSITIVE 316 137 95 82 33 tfor:TF2134|5end_conserved_hypothetical_protein_2308689..2308919_POSITIVE 315 50 3 56 8 tfor:TF0462|5end_possible_cobalamin_biosynthesis_precorrin-3_methylase_477600..477830_POSITIVE 313 140 95 80 36 tfor:TF2108|5end_hypothetical_protein_2277385..2277615_POSITIVE 310 77 35 127 81 tfor:TF0864|5end_O-succinylbenzoate--CoA_ligase_914570..914800_POSITIVE 307 43 1 86 51 tfor:TF0328|5end_conserved_hypothetical_protein_354182..354412_POSITIVE 306 68 21 57 7 tfor:TF0461|5end_ATP:cob(I)alamin_adenosyltransferase_476990..477220_POSITIVE 305 43 13 56 25 tfor:TF1762|5end_hypothetical_protein_1899320..1899550_POSITIVE 305 43 13 41 10 tfor:TF1761|5end_hypothetical_protein_1899305..1899535_POSITIVE 305 52 12 230 183 tfor:TF1757|5end_chromosome_partitioning_protein_1893846..1894076_POSITIVE 304 77 34 75 22 tfor:TF2785|5end_RNA_polymerase_sigma_factor,_ECF_subfamily_(sigma-70)_2969277..2969507_POSITIVE 302 80 33 81 29 tfor:TF1538|5end_conserved_hypothetical_protein_1643531..1643761_POSITIVE 299 134 95 44 6 tfor:TF2165|5end_hypothetical_protein_2341654..2341884_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 366 60 11 65 11 tfor:TF3069|3end_hypothetical_protein_3294413..3294563_POSITIVE 360 125 94 132 100 tfor:TF2336|3end_conserved_hypothetical_protein_2498159..2498309_POSITIVE 342 59 17 61 23 tfor:TF2836|3end_methyltransferase_3028028..3028178_POSITIVE 331 50 2 78 7 tfor:TF0351|3end_hypothetical_protein_371226..371376_POSITIVE 316 137 95 82 33 tfor:TF2132|3end_TIM-barrel_protein,_NifR3_family_2308769..2308919_POSITIVE 313 140 95 51 7 tfor:TF2107|3end_hypothetical_protein_2277436..2277586_POSITIVE 308 68 32 48 3 tfor:TF0812|3end_conserved_hypothetical_protein_872607..872757_POSITIVE 307 43 1 141 106 tfor:TF0326|3end_hypothetical_protein_354317..354467_POSITIVE 306 68 21 57 7 tfor:TF0460|3end_hypothetical_protein_477070..477220_POSITIVE 305 43 13 69 38 tfor:TF1760|3end_guanosine-3',5'-bis(diphosphate)_3'-pyrophosphohydrolase_1899413..1899563_POSITIVE 305 59 13 59 16 tfor:TF0369|3end_hypothetical_protein_386186..386336_POSITIVE 297 64 29 42 5 tfor:TF1488|3end_conserved_hypothetical_protein_1588975..1589125_POSITIVE 295 134 95 147 110 tfor:TF2886|3end_RNA_methylase_SpoU_family_3076270..3076420_POSITIVE 295 63 34 41 15 tfor:TF0828|3end_hypothetical_protein_887508..887658_POSITIVE 294 130 82 120 57 tfor:TF3159|3end_conserved_hypothetical_protein;_possible_transcriptional_regulator_3395611..3395761_POSITIVE 294 50 4 114 61 tfor:TF3022|3end_chorismate_synthase_3247301..3247451_POSITIVE 294 125 81 56 4 tfor:TF2494|3end_NAD-dependent_4-hydroxybutyrate_dehydrogenase_2669520..2669670_POSITIVE 294 54 11 76 42 tfor:TF2099|3end_D-tyrosyl-tRNA(Tyr)_deacylase_2273419..2273569_POSITIVE 287 50 3 59 8 tfor:TF3121|3end_nicotinate_phosphoribosyltransferase_3356124..3356274_POSITIVE 287 122 81 56 18 tfor:TF2188|3end_conserved_hypothetical_protein_2363535..2363685_POSITIVE