Origin IGS: ggattctttctatttaaaattaaaatcatttatacttccatactctgtttagttttatcttgcttatcaagaagcaaaaacaagcagaactccaaataaaaacacaaatttacccacttctattttctataataataattaaagtttttaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttaaaaactttaattattattatagaaaatagaagtgggtaaatttgtgtttttatttggagttctgcttgtttttgcttcttgataagcaagataaaactaaacagagtatggaagtataaatgattttaattttaaatagaaagaatcc Mask Tandem Repeat Region ================================================ ggattctttctatttaaaattaaaatcatttatacttccatactctgtttagttttatcttgcttatcaagaagcaaaaacaagcagaactccaaataaaaacacaaatttacccacttctattttctataataataattaaagtttttaaaa Find is-nt database================================================ Query_seq: TF1485:TF1486|TF1485:TF1486:conserved hypothetical protein:hypothetical protein:->->:1587603..1587755 153 ggattctttctatttaaaattaaaatcatttatacttccatactctgtttagttttatcttgcttatcaagaagcaaaaacaagcagaactccaaataaaaacacaaatttacccacttctattttctataataataattaaagtttttaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1485:TF1486|TF1485:TF1486:conserved hypothetical protein:hypothetical protein:->->:1587603..1587755 153 ggattctttctatttaaaattaaaatcatttatacttccatactctgtttagttttatcttgcttatcaagaagcaaaaacaagcagaactccaaataaaaacacaaatttacccacttctattttctataataataattaaagtttttaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1485:TF1486|TF1485:TF1486:conserved hypothetical protein:hypothetical protein:->->:1587603..1587755 153 ggattctttctatttaaaattaaaatcatttatacttccatactctgtttagttttatcttgcttatcaagaagcaaaaacaagcagaactccaaataaaaacacaaatttacccacttctattttctataataataattaaagtttttaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ggattctttctatttaaaattaaaatcatttatacttccatactctgtttagttttatcttgcttatcaagaagcaaaaacaagcagaactccaaataaaaacacaaatttacccacttctattttctataataataattaaagtttttaaaa Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 45 End: 152 ............................................ M F S F I L L I K K Q K Q A E L Q I K T Q I Y P L L F S I I I I K V F K . ggattctttctatttaaaattaaaatcatttatacttccatactctgtttagttttatcttgcttatcaagaagcaaaaacaagcagaactccaaataaaaacacaaatttacccacttctattttctataataataattaaagtttttaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ggattctttctatttaaaattaaaatcatttatacttccatactctgtttagttttatcttgcttatcaagaagcaaaaacaagcagaactccaaataaaaacacaaatttacccacttctattttctataataataattaaagtttttaaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1485:TF1486|TF1485:TF1486:conserved hypothetical protein:hypothetical protein:->->:1587603..1587755 153 ggattctttctatttaaaattaaaatcatttatacttccatactctgtttagttttatcttgcttatcaagaagcaaaaacaagcagaactccaaataaaaacacaaatttacccacttctattttctataataataattaaagtttttaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 153 Len: 153 Subject: tfor_TF1485_TF1486|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1587603,1587755]|153 HSP 1 e-value: 9.0E-82 bit: 303.0 Len: 153 Query Start:1 Query End:153 Subject Strand: POSITIVE Subject Start: 1 Subject End: 153 ggattctttctatttaaaattaaaatcatttatacttccatactctgtttagttttatcttgcttatcaagaagcaaaaacaagcagaactccaaataaaaacacaaatttacccacttctattttctataataataattaaagtttttaaaa ggattctttctatttaaaattaaaatcatttatacttccatactctgtttagttttatcttgcttatcaagaagcaaaaacaagcagaactccaaataaaaacacaaatttacccacttctattttctataataataattaaagtttttaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGAUUCUUUCUAUUUAAAAUUAAAAUCAUUUAUACUUCCAUACUCUGUUUAGUUUUAUCUUGCUUAUCAAGAAGCAAAAACAAGCAGAACUCCAAAUAAAAACACAAAUUUACCCACUUCUAUUUUCUAUAAUAAUAAUUAAAGUUUUUAAAA (((.........((((....))))...................(((((((.(((((....(((((......)))))))))))))))))..)))...(((((((........................................)))))))... (-15.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUAAAAACUUUAAUUAUUAUUAUAGAAAAUAGAAGUGGGUAAAUUUGUGUUUUUAUUUGGAGUUCUGCUUGUUUUUGCUUCUUGAUAAGCAAGAUAAAACUAAACAGAGUAUGGAAGUAUAAAUGAUUUUAAUUUUAAAUAGAAAGAAUCC .(((((((.....(((((((......(((((((.((((......)))).))))))).(((.(..(((((.(((((((....(((((.....))))).))))).)).)))))..).))).....)))))))....)))))))............ (-22.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 249 96 52 53 7 tfor:TF1026|5end_beta-N-acetylglucosaminidase;_beta_lactamase_1082560..1082790_POSITIVE 246 76 33 165 126 tfor:TF2552|5end_50S_ribosomal_protein_L3_2734758..2734988_POSITIVE 227 96 53 195 146 tfor:TF2758|5end_hypothetical_protein_2942392..2942622_POSITIVE 217 53 25 117 87 tfor:TF2340|5end_hypothetical_protein_2500665..2500895_POSITIVE 216 93 51 80 39 tfor:TF1726|5end_hypothetical_protein_1852049..1852279_POSITIVE 214 126 87 194 157 tfor:TF2315|5end_thioesterase_2473996..2474226_POSITIVE 212 90 41 157 96 tfor:TF0591|5end_RNA_polymerase,_ECF-type_sigma_factor_624578..624808_POSITIVE 210 93 58 37 4 tfor:TF1907|5end_ADP-heptose:LPS_heptosyltransferase_II_2061579..2061809_POSITIVE 202 85 59 136 113 tfor:TF0958|5end_conserved_hypothetical_protein_1003918..1004148_POSITIVE 200 93 55 49 16 tfor:TF3154|5end_conserved_hypothetical_protein_3389219..3389449_POSITIVE 193 94 57 181 147 tfor:TF2071|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_III_2237725..2237955_POSITIVE 193 109 66 171 127 tfor:TF1982|5end_1,4-alpha-glucan_branching_enzyme_2131951..2132181_POSITIVE 193 109 66 171 127 tfor:TF1826|5end_1,4-alpha-glucan_branching_enzyme,_N-terminal_region_1974633..1974863_POSITIVE 193 103 71 132 102 tfor:TF0006|5end_3-oxyacyl_[acyl-carrier_protein]_reductase_1757..1987_POSITIVE 193 103 71 111 81 tfor:TF0004|5end_conserved_hypothetical_protein_1736..1966_POSITIVE 192 88 44 124 85 tfor:TF1076|5end_possible_glycerol_kinase_1145425..1145655_POSITIVE 191 73 35 94 47 tfor:TF1934|5end_hypothetical_protein_2083787..2084017_POSITIVE 191 99 60 61 26 tfor:TF1331|5end_outer_membrane_protein_1409688..1409918_POSITIVE 191 105 70 45 9 tfor:TF0919|5end_conserved_hypothetical_protein_957541..957771_POSITIVE 191 120 74 96 39 tfor:TF0192|5end_hypothetical_protein_207446..207676_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 246 76 33 142 103 tfor:TF2551|3end_30S_ribosomal_protein_S10_2734815..2734965_POSITIVE 227 96 53 58 9 tfor:TF2757|3end_hypothetical_protein_2942335..2942485_POSITIVE 216 93 51 45 4 tfor:TF1725|3end_hypothetical_protein_1852094..1852244_POSITIVE 212 90 41 102 41 tfor:TF0589|3end_anti-sigma_factor_624603..624753_POSITIVE 212 103 62 67 12 tfor:TF0001|3end_hypothetical_protein_158..308_POSITIVE 207 70 25 138 83 tfor:TF1316|3end_conserved_hypothetical_protein;_possible_TPR-repeat-containing_protein_1393496..1393646_POSITIVE 204 88 44 47 3 tfor:TF1485|3end_conserved_hypothetical_protein_1587542..1587692_POSITIVE 200 93 55 74 41 tfor:TF3153|3end_conserved_hypothetical_protein_3389324..3389474_POSITIVE 199 69 36 68 28 tfor:TF2802|3end_possible_outer_membrane_protein_2991814..2991964_POSITIVE 193 109 66 84 40 tfor:TF1981|3end_mannose-6-phosphate_isomerase_2131944..2132094_POSITIVE 193 109 66 84 40 tfor:TF1825|3end_mannose-6-phosphate_isomerase_1974626..1974776_POSITIVE 193 103 71 84 54 tfor:TF0005|3end_possible_beta-lactamase_1789..1939_POSITIVE 191 73 35 108 61 tfor:TF1935|3end_ATP-dependent_RNA_helicase,_DEAD/DEAH-related_helicase_2083881..2084031_POSITIVE 191 99 60 54 19 tfor:TF1330|3end_hypothetical_protein_1409761..1409911_POSITIVE 184 68 28 142 96 tfor:TF1326|3end_hypothetical_protein_1404100..1404250_POSITIVE 183 65 26 109 68 tfor:TF1792|3end_acid_phosphatase_1933319..1933469_POSITIVE 181 77 36 132 83 tfor:TF1638|3end_conserved_hypothetical_protein_1747732..1747882_POSITIVE 180 88 42 42 4 tfor:TF2124|3end_conserved_hypothetical_protein;_possible_TonB_dependent_receptor_2302191..2302341_POSITIVE 180 89 51 104 69 tfor:TF0109|3end_diacylglycerol_kinase_116536..116686_POSITIVE 179 107 61 62 8 tfor:TF2677|3end_aspartate_transaminase_2852079..2852229_POSITIVE