Origin IGS: acgctgatcaagccccctccatgcgcattttaacccaaatcacgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgctttttttcccccaacgcttcgcattgggctgaatgatctccccccccctatttattaatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccattacccgaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggtttaattttcccagttccattgtgttgtaatttattgattagacatattgattagtttttctttggtgtgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacacaccaaagaaaaactaatcaatatgtctaatcaataaattacaacacaatggaactgggaaaattaaacccagattcggcaatagaacaaataaggatgaatttaattcaggccttcagcccttcgaacaaggtccggatgtcgatcccccacccttacgggatggggctgaaataattcgggctttcagccctttcgggtaatggttatgcgtgttacacgctgaatcatgtacccggcatgacaattaaccattaataaatagggggggggagatcattcagcccaatgcgaagcgttgggggaaaaaaagcatcgtatctcggcggagggctgtaagcctgacgtaaccattgtcgtacattcatacgtccattattaatggaaaacgcccaacagtatatcgcgtgatttgggttaaaatgcgcatggagggggcttgatcagcgt Mask Tandem Repeat Region ================================================ acgctgatcaagccccctccatgcgcattttaacccaaatcacgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgctttttttcccccaacgcttcgcattgggctgaatgatctccccccccctatttattaatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccattacccgaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggtttaattttcccagttccattgtgttgtaatttattgattagacatattgattagtttttctttggtgtgtta Find is-nt database================================================ Query_seq: TF1473:TF1474|TF1473:TF1474:hypothetical protein:6-phosphogluconolactonase:->->:1567027..1567481 455 acgctgatcaagccccctccatgcgcattttaacccaaatcacgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgctttttttcccccaacgcttcgcattgggctgaatgatctccccccccctatttattaatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccattacccgaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggtttaattttcccagttccattgtgttgtaatttattgattagacatattgattagtttttctttggtgtgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1473:TF1474|TF1473:TF1474:hypothetical protein:6-phosphogluconolactonase:->->:1567027..1567481 455 acgctgatcaagccccctccatgcgcattttaacccaaatcacgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgctttttttcccccaacgcttcgcattgggctgaatgatctccccccccctatttattaatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccattacccgaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggtttaattttcccagttccattgtgttgtaatttattgattagacatattgattagtttttctttggtgtgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1473:TF1474|TF1473:TF1474:hypothetical protein:6-phosphogluconolactonase:->->:1567027..1567481 455 acgctgatcaagccccctccatgcgcattttaacccaaatcacgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgctttttttcccccaacgcttcgcattgggctgaatgatctccccccccctatttattaatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccattacccgaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggtttaattttcccagttccattgtgttgtaatttattgattagacatattgattagtttttctttggtgtgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acgctgatcaagccccctccatgcgcattttaacccaaatcacgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgctttttttcccccaacgcttcgcattgggctgaatgatctccccccccctatttattaatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccattacccgaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggtttaattttcccagttccattgtgttgtaatttattgattagacatattgattagtttttctttggtgtgtta Predict ORF larger than 30AA ================================================ Protein_Len: 49 Strand: + Start: 47 End: 193 .............................................. M Y C W A F S I N N G R M N V R Q W L R Q A Y S P P P R Y D A F F P P T L R I G L N D L P P P I Y ...................................................................................................................................................................................................................................................................... Protein_Len: 43 Strand: + Start: 204 End: 332 ........................................................................................................................................................................................................... M S C R V H D S A C N T H N H Y P K G L K A R I I S A P S R K G G G S T S G P C S K G ........................................................................................................................... Protein_Len: 47 Strand: - Start: 277 End: 417 .................................................................................................................................................................................................................................................................................... K L G M G Y P H P I S M R V K N S P S F A Q I L N M R I Q E I A S D P N L K G L E M T N Y N M ...................................... Protein_Len: 60 Strand: - Start: 251 End: 430 .......................................................................................................................................................................................................................................................... G F P S F A R I I E A G D R L P P P D V D P G Q E F P Q L G S N F E D K N T R N G F R P K I K G T G N H Q L K N I L C M ......................... Protein_Len: 30 Strand: - Start: 104 End: 193 ....................................................................................................... T L S V A R R R S V I S K K G W R K A N P Q I I E G G R N M ...................................................................................................................................................................................................................................................................... acgctgatcaagccccctccatgcgcattttaacccaaatcacgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgctttttttcccccaacgcttcgcattgggctgaatgatctccccccccctatttattaatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccattacccgaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggtttaattttcccagttccattgtgttgtaatttattgattagacatattgattagtttttctttggtgtgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acgctgatcaagccccctccatgcgcattttaacccaaatcacgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgctttttttcccccaacgcttcgcattgggctgaatgatctccccccccctatttattaatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccattacccgaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggtttaattttcccagttccattgtgttgtaatttattgattagacatattgattagtttttctttggtgtgtta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 72 HP_score: -9.1 Tail_Score: -3.6284 Start: 258 End: 270 Full_Region: gggctgaaataattc gggct ttc agccc tttcgggtaatggtt .................................................................................................................................................................................................................................................................gggctttcagccc......................................................................................................................................................................................... Find igs database================================================ Query_seq: TF1473:TF1474|TF1473:TF1474:hypothetical protein:6-phosphogluconolactonase:->->:1567027..1567481 455 acgctgatcaagccccctccatgcgcattttaacccaaatcacgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgctttttttcccccaacgcttcgcattgggctgaatgatctccccccccctatttattaatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccattacccgaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggtttaattttcccagttccattgtgttgtaatttattgattagacatattgattagtttttctttggtgtgtta Intra-Species Hit: Count: 21 Min: 1 Max: 455 Len: 455 Subject: tfor_TF1473_TF1474|hypothetical protein:6- phosphogluconolactonase|POSITIVE:POSITIVE|[1567027, 1567481]|455 HSP 1 e-value: 0.0 bit: 807.0 Len: 455 Query Start:1 Query End:455 Subject Strand: POSITIVE Subject Start: 1 Subject End: 455 acgctgatcaagccccctccatgcgcattttaacccaaatcacgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgcnnnnnnncccccaacgcttcgcattgggctgaatgatctnnnnnnnnntatttattaatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccattacccgaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggtttaattttcccagttccattgtgttgtaatttattgattagacatattgattagtttttctttggtgtgtta acgctgatcaagccccctccatgcgcattttaacccaaatcacgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgctttttttcccccaacgcttcgcattgggctgaatgatctccccccccctatttattaatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccattacccgaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggtttaattttcccagttccattgtgttgtaatttattgattagacatattgattagtttttctttggtgtgtta Subject: tfor_TF0585_TF0586|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[616824,617452]|629 HSP 1 e-value: 2.0E-67 bit: 258.0 Len: 238 Query Start:147 Query End:384 Subject Strand: NEGATIVE Subject Start: 178 Subject End: 413 ..................................................................................................................................................ccccaacgcttcgcattgggctgaatgatctnnnnnnnnntatttattaatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccattacccgaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggtt....................................................................... ..................................................................................................................................................ccccaacgcttcgcattgggctgaatgatctcgccccc--tatttattaatggttaattgtcatgccgggtacatgattcggcgtgtaacacacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtgggggatccatgcccatatcttggttagagggctgaaggcctgaattaaattcattcttattggttctattgcggaatctgggtt....................................................................... HSP 2 e-value: 9.0E-29 bit: 129.0 Len: 97 Query Start:32 Query End:128 Subject Strand: NEGATIVE Subject Start: 430 Subject End: 526 ...............................aacccaaatcacgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccga....................................................................................................................................................................................................................................................................................................................................... ...............................aacccaaattacgcggtaccctattgggcgtttaccattaataatggacgtacgaatgtacgacaagggttacgtcaggcttacagccctccgccga....................................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF0021_TF0022|outer membrane phospholipase A:two-component system sensor histidine kinase|POSITIVE:POSITIVE|[16980,17434]|455 HSP 1 e-value: 6.0E-64 bit: 246.0 Len: 172 Query Start:195 Query End:366 Subject Strand: POSITIVE Subject Start: 59 Subject End: 230 ..................................................................................................................................................................................................aatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccattacccgaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttc......................................................................................... ..................................................................................................................................................................................................aatggtgaattgtcatgccgggtacatgattcgacgtgtaatgcgcatacccattacccgaaagggccgggagcccgaattatttcagccccatcccgtaagggtggggtatcgacatccggaccttgttcggagggctgaaggcctgaattaaattcattcttatttgttc......................................................................................... Subject: tfor_TF0677_TF0678|ATP-binding protein, Mrp/Nbp35 family, involved in chromosome partitioning:hypothetical protein|POSITIVE:POSITIVE|[721043,721242]|200 HSP 1 e-value: 5.0E-58 bit: 226.0 Len: 177 Query Start:210 Query End:386 Subject Strand: NEGATIVE Subject Start: 6 Subject End: 189 .................................................................................................................................................................................................................tgccgggtacatgattcagcgtgtaacacgcataaccattaccc-------gaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggttta..................................................................... .................................................................................................................................................................................................................tgccgggtacatgattcggcgtgtaccacgcataaccattaaccattgtccgaaagggctgaaagcccgaattatttcagccctatcccgcaagggtggggtatcgacatccggaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgcgtaatctgggttta..................................................................... Subject: tfor_TF3003_TF3004|hypothetical protein:phosphoribosylformylglycinamidine (FGAM) synthase|POSITIVE:POSITIVE|[3220690,3220958]|269 HSP 1 e-value: 5.0E-55 bit: 216.0 Len: 145 Query Start:242 Query End:386 Subject Strand: POSITIVE Subject Start: 29 Subject End: 173 .................................................................................................................................................................................................................................................taaccattacccgaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggttta..................................................................... .................................................................................................................................................................................................................................................taaccattgtccgaaagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatcgacatccggaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgcgtaatctgggttta..................................................................... Subject: tfor_TF2190_TF2191|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2365707,2366073]|367 HSP 1 e-value: 3.0E-53 bit: 210.0 Len: 177 Query Start:210 Query End:386 Subject Strand: NEGATIVE Subject Start: 128 Subject End: 311 .................................................................................................................................................................................................................tgccgggtacatgattcagcgtgtaacacgcataaccattaccc-------gaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggttta..................................................................... .................................................................................................................................................................................................................tgccgggtacatgattcggagtgtaccgcgcataaccattaaccattgtccgaaagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatcgacatccggaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattgcgtaatccgggttta..................................................................... Subject: tfor_TF0179_TF0180|pyrophosphate-energized proton pump:carboxynorspermidine decarboxylase|POSITIVE:POSITIVE|[190752,191406]|655 HSP 1 e-value: 8.0E-51 bit: 202.0 Len: 244 Query Start:29 Query End:272 Subject Strand: POSITIVE Subject Start: 127 Subject End: 378 ............................tttaacccaaatcacgcgatatactgttgggcgttttccatta-----ataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgcnnnnnnncccccaacgcttcgcattgggctgaatgatctnnnnnnnnntatttattaatggttaattgtca-------tgccgggtacatgattcagcgtgtaacacgcataaccattacccgaaagggctgaaagcccga....................................................................................................................................................................................... ............................tttaccccaaattacgcgatacactgttgggcgtttaccattacattaataatgtacgtacgaatgtacgacaagggttacgtcaggcttacagccctccgccgagacgcgatgccttttttccc--aacgcttcgcattgggctgaatgatctcgccccc--tatttattaatggttaattgtcaatggtcatgccgggtacatgattcggcgtgtaacacgcataaccattacccgaaagggctgcaagcccga....................................................................................................................................................................................... HSP 2 e-value: 2.0E-11 bit: 71.9 Len: 61 Query Start:327 Query End:387 Subject Strand: POSITIVE Subject Start: 482 Subject End: 548 ......................................................................................................................................................................................................................................................................................................................................aagggctgaaggcctgaattaaattcatcct------tatttgttctattgccgaatctgggtttaa.................................................................... ......................................................................................................................................................................................................................................................................................................................................aagggctgaaggtctgaattaaattcatccttatttgtatttgttctattgccgaatgtgggtttaa.................................................................... Subject: tfor_TF0821_TF0822|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[880256,880626]|371 HSP 1 e-value: 3.0E-47 bit: 190.0 Len: 140 Query Start:210 Query End:349 Subject Strand: NEGATIVE Subject Start: 200 Subject End: 346 .................................................................................................................................................................................................................tgccgggtacatgattcagcgtgtaacacgcataaccatt-acc------cgaaagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaa.......................................................................................................... .................................................................................................................................................................................................................tgccgggtacatgattcggcgtgtaccacgcataaccattgaccattgtccgaaagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatcgacatccggaccttgttcggagggctgaaggcctgaattaaa.......................................................................................................... HSP 2 e-value: 9.0E-26 bit: 119.0 Len: 92 Query Start:32 Query End:123 Subject Strand: NEGATIVE Subject Start: 110 Subject End: 201 ...............................aacccaaatcacgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctcc............................................................................................................................................................................................................................................................................................................................................ ...............................aacccaaattacgcgatacacttttcggcgtttaccattaataatggatgtgcgaatgtacgacaatggttacgtcaggcttacagccctcc............................................................................................................................................................................................................................................................................................................................................ Subject: tfor_TF2047_TF2048|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2210359,2210782]|424 HSP 1 e-value: 3.0E-44 bit: 180.0 Len: 128 Query Start:257 Query End:384 Subject Strand: POSITIVE Subject Start: 264 Subject End: 393 ................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgtaagggtgggggat--cgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggtt....................................................................... ................................................................................................................................................................................................................................................................agggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatatcgacatccggaccttgttcggagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatctgggtt....................................................................... HSP 2 e-value: 2.0E-32 bit: 141.0 Len: 136 Query Start:42 Query End:177 Subject Strand: POSITIVE Subject Start: 4 Subject End: 137 .........................................acgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgcnnnnnnncccccaacgcttcgcattgggctgaatgatct...................................................................................................................................................................................................................................................................................... .........................................acgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgccttttt--ccccaacgcttcgcattgggctgaattatct...................................................................................................................................................................................................................................................................................... Subject: tfor_TF1483_TF1484|glycosyltransferase family protein:penicillin binding protein|POSITIVE:POSITIVE|[1578886,1579698]|813 HSP 1 e-value: 1.0E-37 bit: 159.0 Len: 88 Query Start:189 Query End:276 Subject Strand: POSITIVE Subject Start: 509 Subject End: 596 ............................................................................................................................................................................................tttattaatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccattacccgaaagggctgaaagcccgaatta................................................................................................................................................................................... ............................................................................................................................................................................................tttattaatggttaattgtcatgccgggtacatgattcggcgtgtaacacgcataaccattacccgaaagggctgaaagcctgaatta................................................................................................................................................................................... HSP 2 e-value: 2.0E-11 bit: 71.9 Len: 52 Query Start:327 Query End:378 Subject Strand: POSITIVE Subject Start: 576 Subject End: 627 ......................................................................................................................................................................................................................................................................................................................................aagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatc............................................................................. ......................................................................................................................................................................................................................................................................................................................................aagggctgaaagcctgaattaaattcattcttatttcttctattgcggaatc............................................................................. Subject: tfor_TF1273_TF1274|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1357523,1357760]|238 HSP 1 e-value: 2.0E-36 bit: 155.0 Len: 144 Query Start:29 Query End:172 Subject Strand: POSITIVE Subject Start: 93 Subject End: 234 ............................tttaacccaaatcacgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgcnnnnnnncccccaacgcttcgcattgggctgaat........................................................................................................................................................................................................................................................................................... ............................tttatcccaaattacgcgatacactgttcggcgtttaccattaatcatggacgtacgaatgcacgacaatggttacgtcaggcttacagccctccgccgggatgcgatgc--cttttcccccaacgcttcgcattgggctgaat........................................................................................................................................................................................................................................................................................... Subject: tfor_TF0029_TF0030|hypothetical protein:N-acetylneuraminate lyase|POSITIVE:POSITIVE|[25123,25909]|787 HSP 1 e-value: 2.0E-36 bit: 155.0 Len: 139 Query Start:34 Query End:172 Subject Strand: NEGATIVE Subject Start: 406 Subject End: 543 .................................cccaaatcacgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgcnnnnnnncccccaacgcttcgcattgggctgaat........................................................................................................................................................................................................................................................................................... .................................cccaaattacgcgatacactgttcggtgtttaccattaataatggacgtacgaatgtacgacaatggttacgtcaagcttacagccctccgccgagatgcgatgcttttttt-ccccaacgcttcgcattgggctgaat........................................................................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-18 bit: 95.6 Len: 60 Query Start:327 Query End:386 Subject Strand: NEGATIVE Subject Start: 10 Subject End: 69 ......................................................................................................................................................................................................................................................................................................................................aagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggttta..................................................................... ......................................................................................................................................................................................................................................................................................................................................aagggctgaaggcctgaattaaattcattcttattggttctattgcggaatctgggttta..................................................................... HSP 3 e-value: 1.0E-18 bit: 95.6 Len: 52 Query Start:256 Query End:307 Subject Strand: NEGATIVE Subject Start: 209 Subject End: 260 ...............................................................................................................................................................................................................................................................aagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatc.................................................................................................................................................... ...............................................................................................................................................................................................................................................................aagggctggaagcccgaattatttcagccccatcccgtaagggtgggggatc.................................................................................................................................................... Subject: tfor_TF1654_TF1655|hypothetical protein:long-chain-fatty-acid-CoA ligase|POSITIVE:POSITIVE|[1768766,1769418]|653 HSP 1 e-value: 1.0E-31 bit: 139.0 Len: 78 Query Start:195 Query End:272 Subject Strand: NEGATIVE Subject Start: 535 Subject End: 612 ..................................................................................................................................................................................................aatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccattacccgaaagggctgaaagcccga....................................................................................................................................................................................... ..................................................................................................................................................................................................aatggttaattgtcatgccgggtacatgattcggcgtgtaacacgcataaccattacccgaaagggctgcaagcccga....................................................................................................................................................................................... HSP 2 e-value: 2.0E-17 bit: 91.7 Len: 58 Query Start:327 Query End:384 Subject Strand: NEGATIVE Subject Start: 390 Subject End: 447 ......................................................................................................................................................................................................................................................................................................................................aagggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggtt....................................................................... ......................................................................................................................................................................................................................................................................................................................................aagggctgaaggcctgaattaaattcattcttattggttctattgcggaatctgggtt....................................................................... Subject: tfor_TF0467_TF0468|acetyltransferase-related protein:hypothetical protein|POSITIVE:POSITIVE|[486604,487068]|465 HSP 1 e-value: 1.0E-27 bit: 125.0 Len: 110 Query Start:29 Query End:138 Subject Strand: POSITIVE Subject Start: 19 Subject End: 129 ............................tttaacccaaatcacgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggc-ttacagccctccgccgagatacgatgc............................................................................................................................................................................................................................................................................................................................. ............................tttatcccaaattacgcgatacattgttcggcgtttaccattaataatggacgtgcgaatgtacgacaatggttacgtcaggcttttcagccctccgccgagatgcgatgc............................................................................................................................................................................................................................................................................................................................. Subject: tfor_TF2025_TF2026|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2182241,2182798]|558 HSP 1 e-value: 9.0E-26 bit: 119.0 Len: 120 Query Start:252 Query End:371 Subject Strand: POSITIVE Subject Start: 102 Subject End: 224 ...........................................................................................................................................................................................................................................................ccgaaagggctgaaa-gcccgaattatttcagccccatcccgt--aagggtgggggatcgacatccggaccttgttcgaagggctgaaggcctgaattaaattcatccttatttgttctattg.................................................................................... ...........................................................................................................................................................................................................................................................ccgaaagggctgaaaagccctagttatttcagccccatcccgcgcaagggtgggataccgatatccagaccttgttcggagggctgaaggcctgaattaaattcattcttatttcttctattg.................................................................................... Subject: tfor_TF0339_TF0340|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[363808,364032]|225 HSP 1 e-value: 1.0E-24 bit: 115.0 Len: 131 Query Start:42 Query End:172 Subject Strand: POSITIVE Subject Start: 4 Subject End: 132 .........................................acgcgatatactgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgagatacgatgcnnnnnnncccccaacgcttcgcattgggctgaat........................................................................................................................................................................................................................................................................................... .........................................acgcgatacacggttcggcgtttaccattaataatgaacgtacgaatgtacgacaagggttacgtcaggcttacagcccttcgccgagacgcgatgccttttt--ccccagcgcttcgcattgggctgaat........................................................................................................................................................................................................................................................................................... Subject: tfor_TF2547_TF2548|hypothetical protein:30S ribosomal protein S12|POSITIVE:POSITIVE|[2731008,2731377]|370 HSP 1 e-value: 1.0E-15 bit: 85.7 Len: 102 Query Start:29 Query End:130 Subject Strand: NEGATIVE Subject Start: 5 Subject End: 107 ............................tttaacccaaatcacgcgatatactgttgggcgttttccat-taataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctccgccgaga..................................................................................................................................................................................................................................................................................................................................... ............................tttatcccaaattacgcgatacaccgttcggcgtttatcatataataatagacgtacgaatatacgacaagggttacgtcagacttacaaccctccgccgaga..................................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF2201_TF2202|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2378345,2378821]|477 HSP 1 e-value: 1.0E-15 bit: 85.7 Len: 82 Query Start:195 Query End:276 Subject Strand: POSITIVE Subject Start: 390 Subject End: 477 ..................................................................................................................................................................................................aatggttaattgtcatgccgggtacatgattcagcgtgtaacacgcataaccatta-------cccgaaagggctgaaagcccgaatta................................................................................................................................................................................... ..................................................................................................................................................................................................aatggttaattgtc-tgccgggtacatgattcggcgtgtaccacgcataaccattaaccattgtccgaaagggctgaaagcccgaatta................................................................................................................................................................................... HSP 2 e-value: 2.0E-11 bit: 71.9 Len: 72 Query Start:52 Query End:123 Subject Strand: POSITIVE Subject Start: 232 Subject End: 303 ...................................................ctgttgggcgttttccattaataatggacgtatgaatgtacgacaatggttacgtcaggcttacagccctcc............................................................................................................................................................................................................................................................................................................................................ ...................................................ctgtttggtgtttaccattaataatggatgtgcaaatgtatgacaatggttatgtcaggcttacagccctcc............................................................................................................................................................................................................................................................................................................................................ HSP 3 e-value: 4.0E-6 bit: 54.0 Len: 31 Query Start:147 Query End:177 Subject Strand: POSITIVE Subject Start: 323 Subject End: 353 ..................................................................................................................................................ccccaacgcttcgcattgggctgaatgatct...................................................................................................................................................................................................................................................................................... ..................................................................................................................................................ccccaacgcttcgcattgggctgaattatct...................................................................................................................................................................................................................................................................................... Subject: tfor_TF1762_TF1763|hypothetical protein:K+-dependent Na+/Ca+ exchanger related-protein|POSITIVE:POSITIVE|[1899709,1900545]|837 HSP 1 e-value: 5.0E-15 bit: 83.8 Len: 66 Query Start:328 Query End:393 Subject Strand: POSITIVE Subject Start: 302 Subject End: 367 .......................................................................................................................................................................................................................................................................................................................................agggctgaaggcctgaattaaattcatccttatttgttctattgccgaatctgggtttaattttcc.............................................................. .......................................................................................................................................................................................................................................................................................................................................agggctgaaggcctgaattaaattcatttttatttcttctattgcggaatccgggtttaatgttcc.............................................................. HSP 2 e-value: 1.0E-12 bit: 75.8 Len: 54 Query Start:256 Query End:309 Subject Strand: POSITIVE Subject Start: 231 Subject End: 284 ...............................................................................................................................................................................................................................................................aagggctgaaagcccgaattatttcagccccatcccgtaagggtgggggatcga.................................................................................................................................................. ...............................................................................................................................................................................................................................................................aagggctgaaagcccgacatatttcagccccatcccgcaagggtggggtatcga.................................................................................................................................................. Subject: tfor_TF3104_TF3105|outer membrane protein, TonB dependent receptor:hypothetical protein|POSITIVE:POSITIVE|[3337511,3338207]|697 HSP 1 e-value: 8.0E-14 bit: 79.8 Len: 48 Query Start:331 Query End:378 Subject Strand: NEGATIVE Subject Start: 446 Subject End: 493 ..........................................................................................................................................................................................................................................................................................................................................gctgaaggcctgaattaaattcatccttatttgttctattgccgaatc............................................................................. ..........................................................................................................................................................................................................................................................................................................................................gctgaaggcctgaattaaattcattcttattggttctattgccgaatc............................................................................. HSP 2 e-value: 5.0E-12 bit: 73.8 Len: 37 Query Start:233 Query End:269 Subject Strand: NEGATIVE Subject Start: 620 Subject End: 656 ........................................................................................................................................................................................................................................taacacgcataaccattacccgaaagggctgaaagcc.......................................................................................................................................................................................... ........................................................................................................................................................................................................................................taacacgcataaccattacccgaaagggctgaaagcc.......................................................................................................................................................................................... Subject: tfor_TF0685_TF0686|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[731193,732338]|1146 HSP 1 e-value: 3.0E-10 bit: 67.9 Len: 38 Query Start:101 Query End:138 Subject Strand: POSITIVE Subject Start: 765 Subject End: 802 ....................................................................................................ttacgtcaggcttacagccctccgccgagatacgatgc............................................................................................................................................................................................................................................................................................................................. ....................................................................................................ttacgtcaggcttacagccctccgccgagatgcgatgc............................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 1.0E-6 bit: 56.0 Len: 48 Query Start:339 Query End:386 Subject Strand: POSITIVE Subject Start: 949 Subject End: 996 ..................................................................................................................................................................................................................................................................................................................................................cctgaattaaattcatccttatttgttctattgccgaatctgggttta..................................................................... ..................................................................................................................................................................................................................................................................................................................................................cctgaattaaattcattcttatttcttctatcgcggaatccgggttta..................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACGCUGAUCAAGCCCCCUCCAUGCGCAUUUUAACCCAAAUCACGCGAUAUACUGUUGGGCGUUUUCCAUUAAUAAUGGACGUAUGAAUGUACGACAAUGGUUACGUCAGGCUUACAGCCCUCCGCCGAGAUACGAUGCUUUUUUUCCCCCAACGCUUCGCAUUGGGCUGAAUGAUCUCCCCCCCCCUAUUUAUUAAUGGUUAAUUGUCAUGCCGGGUACAUGAUUCAGCGUGUAACACGCAUAACCAUUACCCGAAAGGGCUGAAAGCCCGAAUUAUUUCAGCCCCAUCCCGUAAGGGUGGGGGAUCGACAUCCGGACCUUGUUCGAAGGGCUGAAGGCCUGAAUUAAAUUCAUCCUUAUUUGUUCUAUUGCCGAAUCUGGGUUUAAUUUUCCCAGUUCCAUUGUGUUGUAAUUUAUUGAUUAGACAUAUUGAUUAGUUUUUCUUUGGUGUGUUA ...((((((((...........(((((................((((((...(((((..((((((((((.....))))).....)))))..))))).........((((((((.(((((((.((.((((...(((((......(((.(((((.((...)).)))))..))).(((((((((((..........(((((((((...((((((.......))))))...(((((...))))).)))))))))...((..(((((((((..........)))))))))..))......))).))))))))..))).))...))))..)).))))))).)))))))).......................)))))).(((.(((((.........))))))))...))))).........))))))))((((((..(...........)..)))))).. (-140.15) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACACACCAAAGAAAAACUAAUCAAUAUGUCUAAUCAAUAAAUUACAACACAAUGGAACUGGGAAAAUUAAACCCAGAUUCGGCAAUAGAACAAAUAAGGAUGAAUUUAAUUCAGGCCUUCAGCCCUUCGAACAAGGUCCGGAUGUCGAUCCCCCACCCUUACGGGAUGGGGCUGAAAUAAUUCGGGCUUUCAGCCCUUUCGGGUAAUGGUUAUGCGUGUUACACGCUGAAUCAUGUACCCGGCAUGACAAUUAACCAUUAAUAAAUAGGGGGGGGGAGAUCAUUCAGCCCAAUGCGAAGCGUUGGGGGAAAAAAAGCAUCGUAUCUCGGCGGAGGGCUGUAAGCCUGACGUAACCAUUGUCGUACAUUCAUACGUCCAUUAUUAAUGGAAAACGCCCAACAGUAUAUCGCGUGAUUUGGGUUAAAAUGCGCAUGGAGGGGGCUUGAUCAGCGU ..................((.(((((...((((..((.............(((((((..(((((.........)))))..................................((((((...((((((((((....((((.((.((((.((((((((.((((((...((.((((((((((..........)))))))))).))...(((((((((.(((((...)))))....((((((.....))))))....))))))))).....)))))).)))).)))).(((..((((((((...)))))))).))).....)))))).))))...))))))))))...)))))).....))))))).(..((((......(((((.....)))))....((((((...(((.....)))..))))))...))))..)...))..))))))))).))... (-149.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1011 300 252 214 166 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 1011 300 252 214 166 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 944 260 211 198 149 tfor:TF1655|5end_long-chain-fatty-acid-CoA_ligase_1769279..1769509_POSITIVE 935 300 252 200 152 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 917 340 294 91 45 tfor:TF2547|5end_hypothetical_protein_2730664..2730894_POSITIVE 845 120 71 182 133 tfor:TF0823|5end_conserved_hypothetical_protein_880660..880890_POSITIVE 842 170 123 228 183 tfor:TF1187|5end_hypothetical_protein_1269625..1269855_POSITIVE 842 170 123 171 126 tfor:TF1186|5end_hypothetical_protein_1269568..1269798_POSITIVE 842 170 123 167 122 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 838 140 94 47 1 tfor:TF3107|5end_hypothetical_protein_3338459..3338689_POSITIVE 807 307 261 52 7 tfor:TF2840|5end_hypothetical_protein_3031060..3031290_POSITIVE 798 147 101 173 127 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 783 200 151 227 180 tfor:TF2036|5end_possible_pyruvate_formate-lyase_activating_enzyme_2196302..2196532_POSITIVE 782 260 211 212 156 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 773 269 229 169 129 tfor:TF3105|5end_hypothetical_protein_3338068..3338298_POSITIVE 772 286 242 231 187 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 750 360 311 51 2 tfor:TF0820|5end_hypothetical_protein_879656..879886_POSITIVE 695 250 203 162 116 tfor:TF2796|5end_cytochrome_D_ubiquinol_oxidase,_subunit_II_2981269..2981499_POSITIVE 685 160 111 182 137 tfor:TF2478|5end_hypothetical_protein_2655375..2655605_POSITIVE 605 180 136 189 146 tfor:TF0828|5end_hypothetical_protein_887090..887320_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 939 130 81 106 57 tfor:TF3106|3end_hypothetical_protein_3338608..3338758_POSITIVE 876 130 81 86 37 tfor:TF2547|3end_hypothetical_protein_2730947..2731097_POSITIVE 849 350 303 49 1 tfor:TF0677|3end_ATP-binding_protein,_Mrp/Nbp35_family,_involved_in_chromosome_partitioning_720982..721132_POSITIVE 842 170 123 86 41 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 787 256 212 151 107 tfor:TF2840|3end_hypothetical_protein_3031289..3031439_POSITIVE 773 120 71 136 87 tfor:TF0678|3end_hypothetical_protein_721335..721485_POSITIVE 766 170 123 48 3 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 658 180 133 51 5 tfor:TF0820|3end_hypothetical_protein_879942..880092_POSITIVE 642 380 331 75 26 tfor:TF0029|3end_hypothetical_protein_25062..25212_POSITIVE 595 360 317 44 1 tfor:TF0819|3end_phosphoglycerate_mutase_879729..879879_POSITIVE 510 160 111 141 93 tfor:TF1405|3end_conserved_hypothetical_protein_1481819..1481969_POSITIVE 481 150 105 128 79 tfor:TF2816|3end_hypothetical_protein_3007184..3007334_POSITIVE 476 350 306 149 96 tfor:TF0448|3end_hypothetical_protein_467911..468061_POSITIVE 461 150 106 135 88 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 454 323 283 99 58 tfor:TF1342|3end_possible_lipoprotein_1424620..1424770_POSITIVE 431 345 301 73 38 tfor:TF2922|3end_surface_antigen_BspA_3121538..3121688_POSITIVE 428 345 301 85 42 tfor:TF1585|3end_hypothetical_protein_1690610..1690760_POSITIVE 428 345 301 78 35 tfor:TF1584|3end_hypothetical_protein_1690603..1690753_POSITIVE 426 240 193 144 94 tfor:TF1762|3end_hypothetical_protein_1899648..1899798_POSITIVE 426 240 193 75 25 tfor:TF1761|3end_hypothetical_protein_1899579..1899729_POSITIVE