Origin IGS: tcctttccaaaaaataagggcgcaaatttacacgataatcttcaatttccgtaacatacccccggatttcactcctaaacagatgttaatccaaacaatccatagttgtcgaagatatgaaatactttaggaataatatgccggattgcattccttgatacatataatgttttatccaataattttgattatattatttttttttatatctttgcgactaaatccaaccttcagcatattcacatactgatgtagttggtaatatttaataaaaacttttttaacaaaatgaaaaaaaagattaaagacgggctatagtcgtttatctaccactcgactacagttgcatttaaaataattctagcggtaagagaatggggagaatggtaggacttctttgaaatcctattgaatatttattctttgatagaatagattttcagtctattctaagaaatagggggggggggatttgatccccctatttcttaattatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgataattaagaaatagggggatcaaatccccccccccctatttcttagaatagactgaaaatctattctatcaaagaataaatattcaataggatttcaaagaagtcctaccattctccccattctcttaccgctagaattattttaaatgcaactgtagtcgagtggtagataaacgactatagcccgtctttaatcttttttttcattttgttaaaaaagtttttattaaatattaccaactacatcagtatgtgaatatgctgaaggttggatttagtcgcaaagatataaaaaaaaataatataatcaaaattattggataaaacattatatgtatcaaggaatgcaatccggcatattattcctaaagtatttcatatcttcgacaactatggattgtttggattaacatctgtttaggagtgaaatccgggggtatgttacggaaattgaagattatcgtgtaaatttgcgcccttattttttggaaagga Mask Tandem Repeat Region ================================================ tcctttccaaaaaataagggcgcaaatttacacgataatcttcaatttccgtaacatacccccggatttcactcctaaacagatgttaatccaaacaatccatagttgtcgaagatatgaaatactttaggaataatatgccggattgcattccttgatacatataatgttttatccaataattttgattatattatttttttttatatctttgcgactaaatccaaccttcagcatattcacatactgatgtagttggtaatatttaataaaaacttttttaacaaaatgaaaaaaaagattaaagacgggctatagtcgtttatctaccactcgactacagttgcatttaaaataattctagcggtaagagaatggggagaatggtaggacttctttgaaatcctattgaatatttattctttgatagaatagattttcagtctattctaagaaatagggggggggggatttgatccccctatttcttaattatca Find is-nt database================================================ Query_seq: TF1469:TF1470|TF1469:TF1470:2-methylthioadenine synthetase:phosphohydrolase, Icc family:->->:1562786..1563283 498 tcctttccaaaaaataagggcgcaaatttacacgataatcttcaatttccgtaacatacccccggatttcactcctaaacagatgttaatccaaacaatccatagttgtcgaagatatgaaatactttaggaataatatgccggattgcattccttgatacatataatgttttatccaataattttgattatattatttttttttatatctttgcgactaaatccaaccttcagcatattcacatactgatgtagttggtaatatttaataaaaacttttttaacaaaatgaaaaaaaagattaaagacgggctatagtcgtttatctaccactcgactacagttgcatttaaaataattctagcggtaagagaatggggagaatggtaggacttctttgaaatcctattgaatatttattctttgatagaatagattttcagtctattctaagaaatagggggggggggatttgatccccctatttcttaattatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1469:TF1470|TF1469:TF1470:2-methylthioadenine synthetase:phosphohydrolase, Icc family:->->:1562786..1563283 498 tcctttccaaaaaataagggcgcaaatttacacgataatcttcaatttccgtaacatacccccggatttcactcctaaacagatgttaatccaaacaatccatagttgtcgaagatatgaaatactttaggaataatatgccggattgcattccttgatacatataatgttttatccaataattttgattatattatttttttttatatctttgcgactaaatccaaccttcagcatattcacatactgatgtagttggtaatatttaataaaaacttttttaacaaaatgaaaaaaaagattaaagacgggctatagtcgtttatctaccactcgactacagttgcatttaaaataattctagcggtaagagaatggggagaatggtaggacttctttgaaatcctattgaatatttattctttgatagaatagattttcagtctattctaagaaatagggggggggggatttgatccccctatttcttaattatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1469:TF1470|TF1469:TF1470:2-methylthioadenine synthetase:phosphohydrolase, Icc family:->->:1562786..1563283 498 tcctttccaaaaaataagggcgcaaatttacacgataatcttcaatttccgtaacatacccccggatttcactcctaaacagatgttaatccaaacaatccatagttgtcgaagatatgaaatactttaggaataatatgccggattgcattccttgatacatataatgttttatccaataattttgattatattatttttttttatatctttgcgactaaatccaaccttcagcatattcacatactgatgtagttggtaatatttaataaaaacttttttaacaaaatgaaaaaaaagattaaagacgggctatagtcgtttatctaccactcgactacagttgcatttaaaataattctagcggtaagagaatggggagaatggtaggacttctttgaaatcctattgaatatttattctttgatagaatagattttcagtctattctaagaaatagggggggggggatttgatccccctatttcttaattatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tcctttccaaaaaataagggcgcaaatttacacgataatcttcaatttccgtaacatacccccggatttcactcctaaacagatgttaatccaaacaatccatagttgtcgaagatatgaaatactttaggaataatatgccggattgcattccttgatacatataatgttttatccaataattttgattatattatttttttttatatctttgcgactaaatccaaccttcagcatattcacatactgatgtagttggtaatatttaataaaaacttttttaacaaaatgaaaaaaaagattaaagacgggctatagtcgtttatctaccactcgactacagttgcatttaaaataattctagcggtaagagaatggggagaatggtaggacttctttgaaatcctattgaatatttattctttgatagaatagattttcagtctattctaagaaatagggggggggggatttgatccccctatttcttaattatca Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 14 End: 127 ............. M R A Q I Y T I I F N F R N I P P D F T P K Q M L I Q T I H S C R R Y E I L ................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 34 Strand: + Start: 146 End: 247 ................................................................................................................................................. M H S L I H I M F Y P I I L I I L F F F I S L R L N P T F S I F T Y ........................................................................................................................................................................................................................................................... Protein_Len: 40 Strand: + Start: 133 End: 252 .................................................................................................................................... M I C R I A F L D T Y N V L S N N F D Y I I F F Y I F A T K S N L Q H I H I L M ...................................................................................................................................................................................................................................................... Protein_Len: 32 Strand: - Start: 175 End: 270 .............................................................................................................................................................................. G I I K I I N N K K I D K R S F G V K L M N V Y Q H L Q Y Y K M .................................................................................................................................................................................................................................... Protein_Len: 34 Strand: - Start: 78 End: 179 ............................................................................. V S T L G F L G Y N D F I H F V K P I I H R I A N R S V Y L T K D M ............................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: - Start: 31 End: 123 .............................. V I I K L K R L M G G S K V G L C I N I W V I W L Q R L Y S M ....................................................................................................................................................................................................................................................................................................................................................................................... tcctttccaaaaaataagggcgcaaatttacacgataatcttcaatttccgtaacatacccccggatttcactcctaaacagatgttaatccaaacaatccatagttgtcgaagatatgaaatactttaggaataatatgccggattgcattccttgatacatataatgttttatccaataattttgattatattatttttttttatatctttgcgactaaatccaaccttcagcatattcacatactgatgtagttggtaatatttaataaaaacttttttaacaaaatgaaaaaaaagattaaagacgggctatagtcgtttatctaccactcgactacagttgcatttaaaataattctagcggtaagagaatggggagaatggtaggacttctttgaaatcctattgaatatttattctttgatagaatagattttcagtctattctaagaaatagggggggggggatttgatccccctatttcttaattatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 85 Start: 154 End: 183 .........................................................................................................................................................CTTGATACATATAATGTTTTATCCAATAAT........................................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tcctttccaaaaaataagggcgcaaatttacacgataatcttcaatttccgtaacatacccccggatttcactcctaaacagatgttaatccaaacaatccatagttgtcgaagatatgaaatactttaggaataatatgccggattgcattccttgatacatataatgttttatccaataattttgattatattatttttttttatatctttgcgactaaatccaaccttcagcatattcacatactgatgtagttggtaatatttaataaaaacttttttaacaaaatgaaaaaaaagattaaagacgggctatagtcgtttatctaccactcgactacagttgcatttaaaataattctagcggtaagagaatggggagaatggtaggacttctttgaaatcctattgaatatttattctttgatagaatagattttcagtctattctaagaaatagggggggggggatttgatccccctatttcttaattatca Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 71 HP_score: -2.9 Tail_Score: -5.12971 Start: 276 End: 300 Full_Region: tagcccgtctttaat cttttttttca ttt tgttaaaaaag tttttattaaatatt ...................................................................................................................................................................................................................................................................................cttttttttcattttgttaaaaaag...................................................................................................................................................................................................... TransTerm Strand: + Conf: 50 HP_score: -4.5 Tail_Score: -3.17325 Start: 409 End: 443 Full_Region: TTCTTTGAAATCCTA TTGAATATTTATTCT TTGAT AGAATAGATTTTCAG TCTATTCTAAGAAAT ........................................................................................................................................................................................................................................................................................................................................................................................................................TTGAATATTTATTCTTTGATAGAATAGATTTTCAG....................................................... TransTerm Strand: + Conf: 54 HP_score: -5.8 Tail_Score: -2.50074 Start: 410 End: 442 Full_Region: TCTTTGAAATCCTAT TGAATATTTATTCT TTGAT AGAATAGATTTTCA GTCTATTCTAAGAAA .........................................................................................................................................................................................................................................................................................................................................................................................................................TGAATATTTATTCTTTGATAGAATAGATTTTCA........................................................ TransTerm Strand: + Conf: 100 HP_score: -12.3 Tail_Score: -3.78027 Start: 466 End: 482 Full_Region: TAAGAAATAGGGGGG GGGGGAT TTG ATCCCCC TATTTCTTAATTATC .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GGGGGATTTGATCCCCC................ Find igs database================================================ Query_seq: TF1469:TF1470|TF1469:TF1470:2-methylthioadenine synthetase:phosphohydrolase, Icc family:->->:1562786..1563283 498 tcctttccaaaaaataagggcgcaaatttacacgataatcttcaatttccgtaacatacccccggatttcactcctaaacagatgttaatccaaacaatccatagttgtcgaagatatgaaatactttaggaataatatgccggattgcattccttgatacatataatgttttatccaataattttgattatattatttttttttatatctttgcgactaaatccaaccttcagcatattcacatactgatgtagttggtaatatttaataaaaacttttttaacaaaatgaaaaaaaagattaaagacgggctatagtcgtttatctaccactcgactacagttgcatttaaaataattctagcggtaagagaatggggagaatggtaggacttctttgaaatcctattgaatatttattctttgatagaatagattttcagtctattctaagaaatagggggggggggatttgatccccctatttcttaattatca Intra-Species Hit: Count: 1 Min: 1 Max: 498 Len: 498 Subject: tfor_TF1469_TF1470|2-methylthioadenine synthetase:phosphohydrolase, Icc family|POSITIVE:POSITIVE|[1562786,1563283]|498 HSP 1 e-value: 0.0 bit: 821.0 Len: 498 Query Start:1 Query End:498 Subject Strand: POSITIVE Subject Start: 1 Subject End: 498 tcctttccaaaaaataagggcgcaaatttacacgataatcttcaatttccgtaacatacccccggatttcactcctaaacagatgttaatccaaacaatccatagttgtcgaagatatgaaatactttaggaataatatgccggattgcattccttgatacatataatgttttatccaataattttgattatattannnnnnnnnatatctttgcgactaaatccaaccttcagcatattcacatactgatgtagttggtaatatttaataaaaacttttttaacaaaatgnnnnnnnngattaaagacgggctatagtcgtttatctaccactcgactacagttgcatttaaaataattctagcggtaagagaatggggagaatggtaggacttctttgaaatcctattgaatatttattctttgatagaatagattttcagtctattctaagaaatannnnnnnnnnnatttgatccccctatttcttaattatca tcctttccaaaaaataagggcgcaaatttacacgataatcttcaatttccgtaacatacccccggatttcactcctaaacagatgttaatccaaacaatccatagttgtcgaagatatgaaatactttaggaataatatgccggattgcattccttgatacatataatgttttatccaataattttgattatattatttttttttatatctttgcgactaaatccaaccttcagcatattcacatactgatgtagttggtaatatttaataaaaacttttttaacaaaatgaaaaaaaagattaaagacgggctatagtcgtttatctaccactcgactacagttgcatttaaaataattctagcggtaagagaatggggagaatggtaggacttctttgaaatcctattgaatatttattctttgatagaatagattttcagtctattctaagaaatagggggggggggatttgatccccctatttcttaattatca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCCUUUCCAAAAAAUAAGGGCGCAAAUUUACACGAUAAUCUUCAAUUUCCGUAACAUACCCCCGGAUUUCACUCCUAAACAGAUGUUAAUCCAAACAAUCCAUAGUUGUCGAAGAUAUGAAAUACUUUAGGAAUAAUAUGCCGGAUUGCAUUCCUUGAUACAUAUAAUGUUUUAUCCAAUAAUUUUGAUUAUAUUAUUUUUUUUUAUAUCUUUGCGACUAAAUCCAACCUUCAGCAUAUUCACAUACUGAUGUAGUUGGUAAUAUUUAAUAAAAACUUUUUUAACAAAAUGAAAAAAAAGAUUAAAGACGGGCUAUAGUCGUUUAUCUACCACUCGACUACAGUUGCAUUUAAAAUAAUUCUAGCGGUAAGAGAAUGGGGAGAAUGGUAGGACUUCUUUGAAAUCCUAUUGAAUAUUUAUUCUUUGAUAGAAUAGAUUUUCAGUCUAUUCUAAGAAAUAGGGGGGGGGGGAUUUGAUCCCCCUAUUUCUUAAUUAUCA (((((..........))))).............((((((...................(((((((((((((............((((......)))).(((.((((((.(((((((((((((......((((((((((((..(((......))).(((.((((...)))).)))...............)))))))))))))))))))))))))))))))...(((..((((....(((((.(.(((((.((((((((((................(((((((......)))))))..((((....(((........)))....))))...)).))))))))......................))))).).))))).))))..)))..)))......)))))))).((((((.(((((((((.....))))))))).))))))...............))))).(((((((...))))))).........)))))). (-112.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUAAUUAAGAAAUAGGGGGAUCAAAUCCCCCCCCCCCUAUUUCUUAGAAUAGACUGAAAAUCUAUUCUAUCAAAGAAUAAAUAUUCAAUAGGAUUUCAAAGAAGUCCUACCAUUCUCCCCAUUCUCUUACCGCUAGAAUUAUUUUAAAUGCAACUGUAGUCGAGUGGUAGAUAAACGACUAUAGCCCGUCUUUAAUCUUUUUUUUCAUUUUGUUAAAAAAGUUUUUAUUAAAUAUUACCAACUACAUCAGUAUGUGAAUAUGCUGAAGGUUGGAUUUAGUCGCAAAGAUAUAAAAAAAAAUAAUAUAAUCAAAAUUAUUGGAUAAAACAUUAUAUGUAUCAAGGAAUGCAAUCCGGCAUAUUAUUCCUAAAGUAUUUCAUAUCUUCGACAACUAUGGAUUGUUUGGAUUAACAUCUGUUUAGGAGUGAAAUCCGGGGGUAUGUUACGGAAAUUGAAGAUUAUCGUGUAAAUUUGCGCCCUUAUUUUUUGGAAAGGA .......((((((((((((((.............))))))))))))))(((((((.(....))))))))..(((((((((((...((((((((((((((...))))))))..(((...((((.................((((((((........(((((((((..((.....))..)))))))))...((((((...(((((((..(.....)..))))))).....(((((((....((((((...((((((((....)))))))).)))))))))))))...)))))).......))))))))...............((((.........(((((.....(((......))).)))))(((((((((((.........((((..((((((....).))))).)))).........))))))))))).)))).)))).)))........)))))).......(((((....)))))..)))))))))))...... (-119.09) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 412 479 437 148 103 tfor:TF1015|5end_conserved_hypothetical_protein_1072512..1072742_POSITIVE 377 483 443 91 54 tfor:TF1598|5end_conserved_hypothetical_protein_1713358..1713588_POSITIVE 348 490 441 96 49 tfor:TF2681|5end_conserved_hypothetical_protein_2855796..2856026_POSITIVE 347 483 441 160 110 tfor:TF0749|5end_protease_II_795562..795792_POSITIVE 347 484 441 215 183 tfor:TF0133|5end_transposase_element_145174..145404_POSITIVE 343 410 363 148 94 tfor:TF1240|5end_conserved_hypothetical_protein_1324304..1324534_POSITIVE 336 483 450 203 164 tfor:TF2794|5end_possible_transcriptional_regulator_2979938..2980168_POSITIVE 332 489 454 230 190 tfor:TF1260|5end_chaperone_protein_DnaJ_1345325..1345555_POSITIVE 332 480 434 204 158 tfor:TF1182|5end_hypothetical_protein_1268565..1268795_POSITIVE 322 482 444 182 125 tfor:TF0656|5end_acetyltransferase,_possible_GNAT_family_702045..702275_POSITIVE 320 488 448 135 87 tfor:TF1251|5end_riboflavin_synthase,_alpha_subunit_1333744..1333974_POSITIVE 318 395 353 64 17 tfor:TF1921|5end_hypothetical_protein_2070322..2070552_POSITIVE 316 409 364 91 56 tfor:TF0968|5end_hypothetical_protein_1018908..1019138_POSITIVE 313 480 434 139 91 tfor:TF1274|5end_hypothetical_protein_1357621..1357851_POSITIVE 312 86 49 179 142 tfor:TF3018|5end_polyphosphate_kinase_3240122..3240352_POSITIVE 309 406 364 139 93 tfor:TF1904|5end_two-component_system_response_regulator_2058975..2059205_POSITIVE 309 480 453 178 145 tfor:TF1528|5end_malate_dehydrogenase_1633287..1633517_POSITIVE 305 483 452 155 120 tfor:TF2422|5end_hypothetical_protein_2601298..2601528_POSITIVE 304 490 454 215 173 tfor:TF0542|5end_hypothetical_protein_571918..572148_POSITIVE 302 483 459 58 34 tfor:TF1842|5end_hypothetical_protein_1992571..1992801_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 377 483 443 71 34 tfor:TF1597|3end_TatD_family_protein_1713418..1713568_POSITIVE 336 483 450 73 34 tfor:TF2793|3end_possible_L-lysine_2,3-aminomutase_2979888..2980038_POSITIVE 320 488 448 112 64 tfor:TF1250|3end_possible_cation_efflux_pump_1333801..1333951_POSITIVE 309 406 364 139 93 tfor:TF1902|3end_two-component_system_sensor_histidine_kinase_2059055..2059205_POSITIVE 304 490 454 75 33 tfor:TF0541|3end_conserved_hypothetical_protein_571858..572008_POSITIVE 292 347 309 122 72 tfor:TF3021|3end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_3246194..3246344_POSITIVE 290 479 460 30 7 tfor:TF1014|3end_glucose-6-phosphate_isomerase_1072474..1072624_POSITIVE 281 488 444 106 52 tfor:TF0779|3end_possible_outer_membrane_protein_831874..832024_POSITIVE 280 393 356 151 122 tfor:TF2065|3end_CTP:CMP-3-deoxy-D-manno-octulosonate_transferase_2231915..2232065_POSITIVE 275 483 449 88 47 tfor:TF1432|3end_possible_hemolysin_A_1516990..1517140_POSITIVE 275 468 421 66 24 tfor:TF0268|3end_hypothetical_protein_297032..297182_POSITIVE 274 85 41 120 69 tfor:TF2391|3end_conserved_hypothetical_protein_2563464..2563614_POSITIVE 270 473 437 84 46 tfor:TF1713|3end_hypothetical_protein_1833716..1833866_POSITIVE 269 69 32 68 23 tfor:TF2655|3end_DNA_topoisomerase_I_2826962..2827112_POSITIVE 269 472 438 84 56 tfor:TF1342|3end_possible_lipoprotein_1424620..1424770_POSITIVE 267 472 441 149 112 tfor:TF2816|3end_hypothetical_protein_3007184..3007334_POSITIVE 267 392 363 143 110 tfor:TF2181|3end_conserved_hypothetical_protein_2354963..2355113_POSITIVE 264 337 299 91 47 tfor:TF0652|3end_conserved_hypothetical_protein_696522..696672_POSITIVE 262 395 365 45 11 tfor:TF1919|3end_hypothetical_protein_2070383..2070533_POSITIVE 260 149 107 64 22 tfor:TF2897|3end_iron(III)_ABC_transporter,_permease_component_3087096..3087246_POSITIVE