Origin IGS: taacgagacaacgcttttcagacatgatcatttgtcccggagccgctgccccatacggccccgggacatttttatcttctgatgtagtgtgtcagatgttgggacagagagagagtcgtca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgacgactctctctctgtcccaacatctgacacactacatcagaagataaaaatgtcccggggccgtatggggcagcggctccgggacaaatgatcatgtctgaaaagcgttgtctcgtta Mask Tandem Repeat Region ================================================ taacgagacaacgcttttcagacatgatcatttgtcccggagccgctgccccatacggccccgggacatttttatcttctgatgtagtgtgtcagatgttgggacagagagagagtcgtca Find is-nt database================================================ Query_seq: TF1468:TF1469|TF1468:TF1469:beta-galactosidase:2-methylthioadenine synthetase:->->:1561312..1561432 121 taacgagacaacgcttttcagacatgatcatttgtcccggagccgctgccccatacggccccgggacatttttatcttctgatgtagtgtgtcagatgttgggacagagagagagtcgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1468:TF1469|TF1468:TF1469:beta-galactosidase:2-methylthioadenine synthetase:->->:1561312..1561432 121 taacgagacaacgcttttcagacatgatcatttgtcccggagccgctgccccatacggccccgggacatttttatcttctgatgtagtgtgtcagatgttgggacagagagagagtcgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1468:TF1469|TF1468:TF1469:beta-galactosidase:2-methylthioadenine synthetase:->->:1561312..1561432 121 taacgagacaacgcttttcagacatgatcatttgtcccggagccgctgccccatacggccccgggacatttttatcttctgatgtagtgtgtcagatgttgggacagagagagagtcgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacgagacaacgcttttcagacatgatcatttgtcccggagccgctgccccatacggccccgggacatttttatcttctgatgtagtgtgtcagatgttgggacagagagagagtcgtca Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 24 End: 119 ....................... M I I C P G A A A P Y G P G T F L S S D V V C Q M L G Q R E S R .. taacgagacaacgcttttcagacatgatcatttgtcccggagccgctgccccatacggccccgggacatttttatcttctgatgtagtgtgtcagatgttgggacagagagagagtcgtca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacgagacaacgcttttcagacatgatcatttgtcccggagccgctgccccatacggccccgggacatttttatcttctgatgtagtgtgtcagatgttgggacagagagagagtcgtca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -14.0 Tail_Score: -5.16133 Start: 33 End: 68 Full_Region: TCAGACATGATCATT TGTCCCGGAGCCGCTG CCC CATACGGCCCCGGGACA TTTTTATCTTCTGAT ................................TGTCCCGGAGCCGCTGCCCCATACGGCCCCGGGACA..................................................... Find igs database================================================ Query_seq: TF1468:TF1469|TF1468:TF1469:beta-galactosidase:2-methylthioadenine synthetase:->->:1561312..1561432 121 taacgagacaacgcttttcagacatgatcatttgtcccggagccgctgccccatacggccccgggacatttttatcttctgatgtagtgtgtcagatgttgggacagagagagagtcgtca Intra-Species Hit: Count: 1 Min: 1 Max: 121 Len: 121 Subject: tfor_TF1468_TF1469|beta-galactosidase:2-methylthioadenine synthetase|POSITIVE:POSITIVE|[1561312,1561432]|121 HSP 1 e-value: 8.0E-63 bit: 240.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 121 taacgagacaacgcttttcagacatgatcatttgtcccggagccgctgccccatacggccccgggacatttttatcttctgatgtagtgtgtcagatgttgggacagagagagagtcgtca taacgagacaacgcttttcagacatgatcatttgtcccggagccgctgccccatacggccccgggacatttttatcttctgatgtagtgtgtcagatgttgggacagagagagagtcgtca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACGAGACAACGCUUUUCAGACAUGAUCAUUUGUCCCGGAGCCGCUGCCCCAUACGGCCCCGGGACAUUUUUAUCUUCUGAUGUAGUGUGUCAGAUGUUGGGACAGAGAGAGAGUCGUCA (((((.((((.((((..(((((...(((....((((((((.((((..........)))).)))))))).....))).)))))...))))))))...))))).(((.((.......))))). (-41.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGACGACUCUCUCUCUGUCCCAACAUCUGACACACUACAUCAGAAGAUAAAAAUGUCCCGGGGCCGUAUGGGGCAGCGGCUCCGGGACAAAUGAUCAUGUCUGAAAAGCGUUGUCUCGUUA .((((((.((.....((((.........)))).......(((((.(((.....((((((((((((((........))))))))))))))....)))...)))))..)).))))))...... (-46.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 437 60 12 199 148 tfor:TF2931|5end_3-deoxy-D-manno-2-octulosonic_acid_(Kdo_transferase)_3134275..3134505_POSITIVE 433 65 31 218 187 tfor:TF1444|5end_conserved_hypothetical_protein;_possible_hemin_receptor_1528223..1528453_POSITIVE 430 78 32 206 155 tfor:TF1224|5end_hypothetical_protein_1311203..1311433_POSITIVE 404 89 43 195 138 tfor:TF1900|5end_conserved_hypothetical_protein_2053867..2054097_POSITIVE 403 63 24 100 60 tfor:TF1945|5end_glycerate_dehydrogenase_2093229..2093459_POSITIVE 397 79 38 65 13 tfor:TF0298|5end_dipeptidase_323127..323357_POSITIVE 395 68 31 218 176 tfor:TF2611|5end_conserved_hypothetical_protein_2787204..2787434_POSITIVE 391 66 29 184 131 tfor:TF2434|5end_conserved_hypothetical_protein_2613969..2614199_POSITIVE 384 79 36 125 73 tfor:TF2246|5end_hypothetical_protein_2421288..2421518_POSITIVE 383 66 21 221 167 tfor:TF0018|5end_hypothetical_protein_11250..11480_POSITIVE 381 61 24 39 9 tfor:TF1616|5end_conserved_hypothetical_protein;_possible_transcriptional_regulator_1732491..1732721_POSITIVE 379 67 23 197 142 tfor:TF0971|5end_conserved_hypothetical_protein_1021355..1021585_POSITIVE 378 59 12 220 170 tfor:TF2561|5end_50S_ribosomal_protein_L16_2738693..2738923_POSITIVE 378 64 22 229 161 tfor:TF2380|5end_hemagglutinin_2545012..2545242_POSITIVE 373 64 23 204 156 tfor:TF1808|5end_DNA_processing_protein_subunit_A_1949356..1949586_POSITIVE 372 65 21 59 6 tfor:TF2817|5end_hypothetical_protein_3007358..3007588_POSITIVE 372 65 21 214 161 tfor:TF1845|5end_hypothetical_protein_1996620..1996850_POSITIVE 372 65 21 214 161 tfor:TF1185|5end_hypothetical_protein_1269238..1269468_POSITIVE 371 74 31 210 151 tfor:TF0949|5end_conserved_hypothetical_protein_993863..994093_POSITIVE 361 65 23 204 156 tfor:TF1848|5end_conserved_hypothetical_protein_1997589..1997819_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 437 68 33 58 23 tfor:TF1468|3end_beta-galactosidase_1561251..1561401_POSITIVE 433 65 31 118 87 tfor:TF1443|3end_hypothetical_protein_1528203..1528353_POSITIVE 415 80 32 148 96 tfor:TF0279|3end_conserved_hypothetical_protein_308679..308829_POSITIVE 404 89 43 63 6 tfor:TF1899|3end_anti-sigma_factor_2053815..2053965_POSITIVE 403 63 24 100 60 tfor:TF1944|3end_conserved_hypothetical_protein_2093309..2093459_POSITIVE 383 66 21 122 68 tfor:TF0017|3end_hypothetical_protein_11231..11381_POSITIVE 381 61 24 39 9 tfor:TF1614|3end_conserved_hypothetical_protein_1732571..1732721_POSITIVE 356 49 4 86 32 tfor:TF2789|3end_enoyl-[acyl-carrier-protein]_reductase_2973365..2973515_POSITIVE 356 66 21 95 31 tfor:TF0252|3end_hypothetical_protein_283759..283909_POSITIVE 354 59 11 126 83 tfor:TF1622|3end_conserved_hypothetical_protein_1736763..1736913_POSITIVE 353 59 12 151 98 tfor:TF2745|3end_hypothetical_protein_2927947..2928097_POSITIVE 349 69 35 86 55 tfor:TF2153|3end_6-phosphofructokinase_2329172..2329322_POSITIVE 348 64 21 59 7 tfor:TF1110|3end_conserved_hypothetical_protein_1180837..1180987_POSITIVE 347 80 33 110 53 tfor:TF0144|3end_hypothetical_protein_151483..151633_POSITIVE 344 62 32 43 8 tfor:TF2883|3end_peptidyl-prolyl_cis-trans_isomerase_3075129..3075279_POSITIVE 344 63 21 148 100 tfor:TF2795|3end_hypothetical_protein_2980909..2981059_POSITIVE 344 58 13 134 77 tfor:TF2021|3end_hypothetical_protein_2178588..2178738_POSITIVE 343 67 23 76 28 tfor:TF3107|3end_hypothetical_protein_3338736..3338886_POSITIVE 339 68 22 147 94 tfor:TF3021|3end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_3246194..3246344_POSITIVE 338 79 32 151 96 tfor:TF2488|3end_hypothetical_protein_2664478..2664628_POSITIVE