Origin IGS: atataactattatcgtttgatttttttaccggccaaaggtacgtgatcatcttttttaacggtcgactctcccgccggtttcaggctgctcggctccatttgaacagacttgcatacgactaatcgaacatcgtaaataccatttacaatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgattgtaaatggtatttacgatgttcgattagtcgtatgcaagtctgttcaaatggagccgagcagcctgaaaccggcgggagagtcgaccgttaaaaaagatgatcacgtacctttggccggtaaaaaaatcaaacgataatagttatat Mask Tandem Repeat Region ================================================ atataactattatcgtttgatttttttaccggccaaaggtacgtgatcatcttttttaacggtcgactctcccgccggtttcaggctgctcggctccatttgaacagacttgcatacgactaatcgaacatcgtaaataccatttacaatca Find is-nt database================================================ Query_seq: TF1436:TF1437|TF1436:TF1437:phosphoglycolate phosphatase:hypothetical protein:->->:1521928..1522079 152 atataactattatcgtttgatttttttaccggccaaaggtacgtgatcatcttttttaacggtcgactctcccgccggtttcaggctgctcggctccatttgaacagacttgcatacgactaatcgaacatcgtaaataccatttacaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1436:TF1437|TF1436:TF1437:phosphoglycolate phosphatase:hypothetical protein:->->:1521928..1522079 152 atataactattatcgtttgatttttttaccggccaaaggtacgtgatcatcttttttaacggtcgactctcccgccggtttcaggctgctcggctccatttgaacagacttgcatacgactaatcgaacatcgtaaataccatttacaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1436:TF1437|TF1436:TF1437:phosphoglycolate phosphatase:hypothetical protein:->->:1521928..1522079 152 atataactattatcgtttgatttttttaccggccaaaggtacgtgatcatcttttttaacggtcgactctcccgccggtttcaggctgctcggctccatttgaacagacttgcatacgactaatcgaacatcgtaaataccatttacaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atataactattatcgtttgatttttttaccggccaaaggtacgtgatcatcttttttaacggtcgactctcccgccggtttcaggctgctcggctccatttgaacagacttgcatacgactaatcgaacatcgtaaataccatttacaatca Predict ORF larger than 30AA ================================================ Protein_Len: 46 Strand: - Start: 13 End: 150 ............ R K I K K G A L P V H D D K K V T S E G A P K L S S P E M Q V S K C V V L R V D Y I G N V M .. atataactattatcgtttgatttttttaccggccaaaggtacgtgatcatcttttttaacggtcgactctcccgccggtttcaggctgctcggctccatttgaacagacttgcatacgactaatcgaacatcgtaaataccatttacaatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atataactattatcgtttgatttttttaccggccaaaggtacgtgatcatcttttttaacggtcgactctcccgccggtttcaggctgctcggctccatttgaacagacttgcatacgactaatcgaacatcgtaaataccatttacaatca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1436:TF1437|TF1436:TF1437:phosphoglycolate phosphatase:hypothetical protein:->->:1521928..1522079 152 atataactattatcgtttgatttttttaccggccaaaggtacgtgatcatcttttttaacggtcgactctcccgccggtttcaggctgctcggctccatttgaacagacttgcatacgactaatcgaacatcgtaaataccatttacaatca Intra-Species Hit: Count: 1 Min: 1 Max: 152 Len: 152 Subject: tfor_TF1436_TF1437|phosphoglycolate phosphatase:hypothetical protein|POSITIVE:POSITIVE|[1521928,1522079]|152 HSP 1 e-value: 1.0E-68 bit: 260.0 Len: 152 Query Start:1 Query End:152 Subject Strand: POSITIVE Subject Start: 1 Subject End: 152 atataactattatcgtttgannnnnnnaccggccaaaggtacgtgatcatcttttttaacggtcgactctcccgccggtttcaggctgctcggctccatttgaacagacttgcatacgactaatcgaacatcgtaaataccatttacaatca atataactattatcgtttgatttttttaccggccaaaggtacgtgatcatcttttttaacggtcgactctcccgccggtttcaggctgctcggctccatttgaacagacttgcatacgactaatcgaacatcgtaaataccatttacaatca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAUAACUAUUAUCGUUUGAUUUUUUUACCGGCCAAAGGUACGUGAUCAUCUUUUUUAACGGUCGACUCUCCCGCCGGUUUCAGGCUGCUCGGCUCCAUUUGAACAGACUUGCAUACGACUAAUCGAACAUCGUAAAUACCAUUUACAAUCA ..............((((((((.....((((((....((....(((((............))))).....)).))))))..(((((((.((((......)))).))).)))).........))))))))...((((((...))))))..... (-30.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUUGUAAAUGGUAUUUACGAUGUUCGAUUAGUCGUAUGCAAGUCUGUUCAAAUGGAGCCGAGCAGCCUGAAACCGGCGGGAGAGUCGACCGUUAAAAAAGAUGAUCACGUACCUUUGGCCGGUAAAAAAAUCAAACGAUAAUAGUUAUAU ..(((((((((...)))))))))....((((((((((.((..((.((((((..........)))))).))...((((((.((((.((((..((((......))))....)).)))))).))))))........)).)))))..))))).... (-36.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 423 100 60 189 150 tfor:TF1785|5end_histidine_ammonia-lyase_1924494..1924724_POSITIVE 409 113 71 56 3 tfor:TF2876|5end_cytosine_deaminase_3065144..3065374_POSITIVE 409 89 41 66 21 tfor:TF2833|5end_lipid_A_disaccharide_synthase_3023973..3024203_POSITIVE 404 119 72 143 102 tfor:TF2844|5end_possible_phosphatase_or_hydrolase_3033680..3033910_POSITIVE 393 110 62 174 106 tfor:TF0038|5end_conserved_hypothetical_protein_39907..40137_POSITIVE 388 107 62 231 176 tfor:TF1064|5end_ampG_permease_1134004..1134234_POSITIVE 385 118 71 112 65 tfor:TF1239|5end_RNA_polymerase_ECF-type_sigma_factor_1323580..1323810_POSITIVE 382 89 42 189 145 tfor:TF3141|5end_NADH:_ubiquinone_oxidoreductase,_subunit_A_3374667..3374897_POSITIVE 381 106 61 165 133 tfor:TF2886|5end_RNA_methylase_SpoU_family_3075651..3075881_POSITIVE 371 119 72 175 128 tfor:TF2820|5end_argininosuccinate_synthetase_3009876..3010106_POSITIVE 368 120 71 91 34 tfor:TF2477|5end_phosphatase_2654229..2654459_POSITIVE 368 97 62 166 125 tfor:TF0819|5end_phosphoglycerate_mutase_878447..878677_POSITIVE 366 94 58 133 101 tfor:TF2187|5end_conserved_hypothetical_protein_2361693..2361923_POSITIVE 366 97 56 147 91 tfor:TF2151|5end_uridine_kinase_2323033..2323263_POSITIVE 366 96 60 49 5 tfor:TF0462|5end_possible_cobalamin_biosynthesis_precorrin-3_methylase_477600..477830_POSITIVE 365 93 57 81 30 tfor:TF2108|5end_hypothetical_protein_2277385..2277615_POSITIVE 364 113 73 225 192 tfor:TF1444|5end_conserved_hypothetical_protein;_possible_hemin_receptor_1528223..1528453_POSITIVE 363 98 59 183 134 tfor:TF2642|5end_acetylornithine_deacetylase_2810677..2810907_POSITIVE 363 94 60 40 2 tfor:TF2301|5end_outer_membrane_protein,_TonB_dependent_receptor_2453288..2453518_POSITIVE 363 118 74 77 29 tfor:TF1062|5end_glycosyltransferase_1131727..1131957_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 423 94 60 126 89 tfor:TF0350|3end_hypothetical_protein_371101..371251_POSITIVE 409 113 71 56 3 tfor:TF2874|3end_conserved_hypothetical_protein_3065224..3065374_POSITIVE 404 119 72 143 102 tfor:TF2843|3end_conserved_hypothetical_protein;_possible_lipoprotein_3033760..3033910_POSITIVE 385 118 71 51 4 tfor:TF1237|3end_possible_L-fuculose_kinase_1323599..1323749_POSITIVE 372 94 60 144 107 tfor:TF1215|3end_haloacid_dehalogenase-like_hydrolase_1303216..1303366_POSITIVE 366 118 73 150 102 tfor:TF2548|3end_30S_ribosomal_protein_S12_2731704..2731854_POSITIVE 366 97 56 144 88 tfor:TF2149|3end_deoxyribose-phosphate_aldolase_2323110..2323260_POSITIVE 365 93 57 52 1 tfor:TF2107|3end_hypothetical_protein_2277436..2277586_POSITIVE 364 113 73 125 92 tfor:TF1443|3end_hypothetical_protein_1528203..1528353_POSITIVE 363 118 74 77 29 tfor:TF1061|3end_glycosyltransferase_1131807..1131957_POSITIVE 361 93 60 149 119 tfor:TF1908|3end_possible_phosphoglycerate_mutase_2063299..2063449_POSITIVE 359 100 51 122 54 tfor:TF1775|3end_oxidoreductase,_Gfo/Idh/MocA_family_1914618..1914768_POSITIVE 351 98 59 54 12 tfor:TF2160|3end_magnesium_chelatase,_subunit_I_2337049..2337199_POSITIVE 343 98 54 122 76 tfor:TF2255|3end_transfer_region-related_protein,_TraH_2427961..2428111_POSITIVE 340 94 53 150 108 tfor:TF1191|3end_possible_transcriptional_regulator_1273979..1274129_POSITIVE 335 96 59 135 94 tfor:TF3021|3end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_3246194..3246344_POSITIVE 330 104 61 52 11 tfor:TF2949|3end_dihydroxynaphthoate_synthase_(naphthoate_synthase)_3157479..3157629_POSITIVE 329 100 58 130 69 tfor:TF2853|3end_2-C-methyl-D-erythritol_2,4-cyclodiphosphate_synthase_3040725..3040875_POSITIVE 327 94 59 141 96 tfor:TF0676|3end_methyltransferase_719865..720015_POSITIVE 326 108 64 66 21 tfor:TF2266|3end_transfer_region-related_protein,_TraQ_2434949..2435099_POSITIVE