Origin IGS: taaatgaatatccacatgtacatagggctaatagcatcttattactttgtcacaacaatttacatctgattgtcgattgattatcaatggatataggggctaatccccccccggggagaaacaaagaaaggataatgtaaaccgattaggggaatgtatgaatatggatttcatttaacttttttatgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcattaacttttttattatagtacc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ggtactataataaaaaagttaatgaatatccgtgtttgtacctaaaaactccattaaactatttaataacaataatttggatgcatggattggagtaagaaataaatttctgtagattagcaatatataattaattttaggcatatatacggacattcataaaaaagttaaatgaaatccatattcatacattcccctaatcggtttacattatcctttctttgtttctccccggggggggattagcccctatatccattgataatcaatcgacaatcagatgtaaattgttgtgacaaagtaataagatgctattagccctatgtacatgtggatattcattta Mask Tandem Repeat Region ================================================ taaatgaatatccacatgtacatagggctaatagcatcttattactttgtcacaacaatttacatctgattgtcgattgattatcaatggatataggggctaatccccccccggggagaaacaaagaaaggataatgtaaaccgattaggggaatgtatgaatatggatttcatttaacttttttatgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcattaacttttttattatagtacc Find is-nt database================================================ Query_seq: TF1369:TF1370|TF1369:TF1370:hypothetical protein:conserved hypothetical protein:->->:1447647..1447991 345 taaatgaatatccacatgtacatagggctaatagcatcttattactttgtcacaacaatttacatctgattgtcgattgattatcaatggatataggggctaatccccccccggggagaaacaaagaaaggataatgtaaaccgattaggggaatgtatgaatatggatttcatttaacttttttatgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcattaacttttttattatagtacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1369:TF1370|TF1369:TF1370:hypothetical protein:conserved hypothetical protein:->->:1447647..1447991 345 taaatgaatatccacatgtacatagggctaatagcatcttattactttgtcacaacaatttacatctgattgtcgattgattatcaatggatataggggctaatccccccccggggagaaacaaagaaaggataatgtaaaccgattaggggaatgtatgaatatggatttcatttaacttttttatgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcattaacttttttattatagtacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1369:TF1370|TF1369:TF1370:hypothetical protein:conserved hypothetical protein:->->:1447647..1447991 345 taaatgaatatccacatgtacatagggctaatagcatcttattactttgtcacaacaatttacatctgattgtcgattgattatcaatggatataggggctaatccccccccggggagaaacaaagaaaggataatgtaaaccgattaggggaatgtatgaatatggatttcatttaacttttttatgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcattaacttttttattatagtacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaatgaatatccacatgtacatagggctaatagcatcttattactttgtcacaacaatttacatctgattgtcgattgattatcaatggatataggggctaatccccccccggggagaaacaaagaaaggataatgtaaaccgattaggggaatgtatgaatatggatttcatttaacttttttatgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcattaacttttttattatagtacc Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 190 End: 324 ............................................................................................................................................................................................. M S V Y M P K I N Y I L L I Y R N L F L T P I H A S K L L L L N S L M E F L G T N T D I H ..................... taaatgaatatccacatgtacatagggctaatagcatcttattactttgtcacaacaatttacatctgattgtcgattgattatcaatggatataggggctaatccccccccggggagaaacaaagaaaggataatgtaaaccgattaggggaatgtatgaatatggatttcatttaacttttttatgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcattaacttttttattatagtacc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaatgaatatccacatgtacatagggctaatagcatcttattactttgtcacaacaatttacatctgattgtcgattgattatcaatggatataggggctaatccccccccggggagaaacaaagaaaggataatgtaaaccgattaggggaatgtatgaatatggatttcatttaacttttttatgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcattaacttttttattatagtacc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1369:TF1370|TF1369:TF1370:hypothetical protein:conserved hypothetical protein:->->:1447647..1447991 345 taaatgaatatccacatgtacatagggctaatagcatcttattactttgtcacaacaatttacatctgattgtcgattgattatcaatggatataggggctaatccccccccggggagaaacaaagaaaggataatgtaaaccgattaggggaatgtatgaatatggatttcatttaacttttttatgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcattaacttttttattatagtacc Intra-Species Hit: Count: 7 Min: 1 Max: 345 Len: 345 Subject: tfor_TF1369_TF1370|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1447647,1447991]|345 HSP 1 e-value: 0.0 bit: 636.0 Len: 345 Query Start:1 Query End:345 Subject Strand: POSITIVE Subject Start: 1 Subject End: 345 taaatgaatatccacatgtacatagggctaatagcatcttattactttgtcacaacaatttacatctgattgtcgattgattatcaatggatataggggctaatnnnnnnnnggggagaaacaaagaaaggataatgtaaaccgattaggggaatgtatgaatatggatttcatttaacttttttatgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcattaacttttttattatagtacc taaatgaatatccacatgtacatagggctaatagcatcttattactttgtcacaacaatttacatctgattgtcgattgattatcaatggatataggggctaatccccccccggggagaaacaaagaaaggataatgtaaaccgattaggggaatgtatgaatatggatttcatttaacttttttatgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcattaacttttttattatagtacc Subject: tfor_TF2966_TF2967|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[3177733,3177904]|172 HSP 1 e-value: 8.0E-75 bit: 281.0 Len: 142 Query Start:184 Query End:325 Subject Strand: NEGATIVE Subject Start: 14 Subject End: 155 .......................................................................................................................................................................................ttatgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcatt.................... .......................................................................................................................................................................................ttatgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcatt.................... Subject: tfor_TF2175_TF2176|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2348876,2349050]|175 HSP 1 e-value: 3.0E-74 bit: 280.0 Len: 141 Query Start:187 Query End:327 Subject Strand: NEGATIVE Subject Start: 35 Subject End: 175 ..........................................................................................................................................................................................tgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcattaa.................. ..........................................................................................................................................................................................tgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcattaa.................. Subject: tfor_TF1890_TF1891|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2043154,2043528]|375 HSP 1 e-value: 3.0E-74 bit: 280.0 Len: 141 Query Start:185 Query End:325 Subject Strand: NEGATIVE Subject Start: 5 Subject End: 145 ........................................................................................................................................................................................tatgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcatt.................... ........................................................................................................................................................................................tatgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcatt.................... Subject: tfor_TF2478_TF2479|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2655674,2656131]|458 HSP 1 e-value: 6.0E-48 bit: 192.0 Len: 133 Query Start:189 Query End:321 Subject Strand: POSITIVE Subject Start: 296 Subject End: 427 ............................................................................................................................................................................................aatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatatt........................ ............................................................................................................................................................................................aatgtccgtatatatgcctaaaattaattatatattgctgatatacagaaatttattccctcc-ccaatccatacatccaaattattgttattaaatagtttaatggagcttttaggtacaaacacggatatt........................ Subject: tfor_TF2162_TF2163|thermolysin precursor:hypothetical protein|POSITIVE:POSITIVE|[2340774,2340941]|168 HSP 1 e-value: 2.0E-41 bit: 170.0 Len: 118 Query Start:186 Query End:303 Subject Strand: POSITIVE Subject Start: 51 Subject End: 168 .........................................................................................................................................................................................atgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagttttta.......................................... .........................................................................................................................................................................................atgaatgtccgtatatatgcctaaaattaattatatattactgatatacagaaatttatctcttgcttcaatccatacatccaaattattgttattaaatagtttaatggagctttta.......................................... Subject: tfor_TF0899_TF0901|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[946220,946820]|601 HSP 1 e-value: 2.0E-41 bit: 170.0 Len: 142 Query Start:186 Query End:327 Subject Strand: NEGATIVE Subject Start: 409 Subject End: 550 .........................................................................................................................................................................................atgaatgtccgtatatatgcctaaaattaattatatattgctaatctacagaaatttatttcttactccaatccatgcatccaaattattgttattaaatagtttaatggagtttttaggtacaaacacggatattcattaa.................. .........................................................................................................................................................................................atgaatgtccgtatatatgcctaaaattaattatatattgctgatatacagaaatctatttctctctccgacctatacatctaaattattgttgttaaatagtttaatagagtttttaggtgcaaaaacggatattcattaa.................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUGAAUAUCCACAUGUACAUAGGGCUAAUAGCAUCUUAUUACUUUGUCACAACAAUUUACAUCUGAUUGUCGAUUGAUUAUCAAUGGAUAUAGGGGCUAAUCCCCCCCCGGGGAGAAACAAAGAAAGGAUAAUGUAAACCGAUUAGGGGAAUGUAUGAAUAUGGAUUUCAUUUAACUUUUUUAUGAAUGUCCGUAUAUAUGCCUAAAAUUAAUUAUAUAUUGCUAAUCUACAGAAAUUUAUUUCUUACUCCAAUCCAUGCAUCCAAAUUAUUGUUAUUAAAUAGUUUAAUGGAGUUUUUAGGUACAAACACGGAUAUUCAUUAACUUUUUUAUUAUAGUACC ..(((((((((((...(((((..(((((.....((((....((((.(((((.............(((...((((.((((.....)))).))))..))).....(((((....))))).............))))).))))...((((.((((...(((((.(((((((((((((...........))))).)))))))).))))).............................(((((....)))))..)))))))).)))).(((((((((((.......)))))))...)))))))))...))))).....))))))))))).................... (-75.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUACUAUAAUAAAAAAGUUAAUGAAUAUCCGUGUUUGUACCUAAAAACUCCAUUAAACUAUUUAAUAACAAUAAUUUGGAUGCAUGGAUUGGAGUAAGAAAUAAAUUUCUGUAGAUUAGCAAUAUAUAAUUAAUUUUAGGCAUAUAUACGGACAUUCAUAAAAAAGUUAAAUGAAAUCCAUAUUCAUACAUUCCCCUAAUCGGUUUACAUUAUCCUUUCUUUGUUUCUCCCCGGGGGGGGAUUAGCCCCUAUAUCCAUUGAUAAUCAAUCGACAAUCAGAUGUAAAUUGUUGUGACAAAGUAAUAAGAUGCUAUUAGCCCUAUGUACAUGUGGAUAUUCAUUUA .....................((((((.(((((((.(((.(((((((((((((......(((((((..........))))))).......)))))).(((((....)))))...(((((........)))))..))))))).))).))))))).))))))........((((((((((((((((...(((((....(((((.(((.....((((.....(((((((((((((....)))))).((((...((((((...((((..((....))..))))))))))....)))).)))))))..))))...))))))))....))))).)))))))).)))))))) (-76.72) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 771 270 221 195 146 tfor:TF2967|5end_hypothetical_protein_3177765..3177995_POSITIVE 771 270 221 175 126 tfor:TF2176|5end_hypothetical_protein_2348911..2349141_POSITIVE 594 240 191 197 148 tfor:TF0901|5end_conserved_hypothetical_protein_946681..946911_POSITIVE 386 115 74 187 152 tfor:TF2086|5end_transglutaminase-related_protein_2253931..2254161_POSITIVE 331 112 71 127 87 tfor:TF1470|5end_phosphohydrolase,_Icc_family_1563144..1563374_POSITIVE 310 226 186 211 172 tfor:TF2304|5end_OmpA_family_protein_2459320..2459550_POSITIVE 310 114 75 59 30 tfor:TF1762|5end_hypothetical_protein_1899320..1899550_POSITIVE 310 114 75 44 15 tfor:TF1761|5end_hypothetical_protein_1899305..1899535_POSITIVE 308 140 92 118 71 tfor:TF2540|5end_conserved_hypothetical_protein_2723058..2723288_POSITIVE 307 118 72 49 1 tfor:TF3087|5end_conserved_hypothetical_protein_3319878..3320108_POSITIVE 307 124 89 116 81 tfor:TF0495|5end_conserved_hypothetical_protein;_possible_S-layer_related_protein_precursor_520862..521092_POSITIVE 301 118 71 133 79 tfor:TF0340|5end_hypothetical_protein_363893..364123_POSITIVE 298 107 64 183 143 tfor:TF1882|5end_conserved_hypothetical_protein_2033987..2034217_POSITIVE 297 119 71 225 179 tfor:TF3147|5end_adenylate_kinase_3383049..3383279_POSITIVE 295 109 63 60 12 tfor:TF2341|5end_hypothetical_protein_2505735..2505965_POSITIVE 294 119 76 126 90 tfor:TF3132|5end_conserved_hypothetical_protein_3365175..3365405_POSITIVE 294 118 71 133 95 tfor:TF0366|5end_hypothetical_protein_383564..383794_POSITIVE 294 118 71 133 95 tfor:TF0362|5end_hypothetical_protein_379952..380182_POSITIVE 294 118 71 133 95 tfor:TF0346|5end_hypothetical_protein_367048..367278_POSITIVE 290 126 84 205 147 tfor:TF0656|5end_acetyltransferase,_possible_GNAT_family_702045..702275_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 737 300 251 52 3 tfor:TF2966|3end_hypothetical_protein_3177672..3177822_POSITIVE 737 300 251 61 12 tfor:TF1890|3end_hypothetical_protein_2043093..2043243_POSITIVE 682 320 272 49 1 tfor:TF2175|3end_hypothetical_protein_2348815..2348965_POSITIVE 325 140 95 58 17 tfor:TF0018|3end_hypothetical_protein_12091..12241_POSITIVE 310 226 186 114 75 tfor:TF3047|3end_transposase_3276336..3276486_POSITIVE 310 226 186 114 75 tfor:TF2303|3end_conserved_hypothetical_protein_2459303..2459453_POSITIVE 310 114 75 72 43 tfor:TF1760|3end_guanosine-3',5'-bis(diphosphate)_3'-pyrophosphohydrolase_1899413..1899563_POSITIVE 310 226 186 114 75 tfor:TF0941|3end_transposase_fragment_982313..982463_POSITIVE 308 140 92 96 49 tfor:TF2539|3end_hypothetical_protein_2723116..2723266_POSITIVE 307 138 95 79 36 tfor:TF2922|3end_surface_antigen_BspA_3121538..3121688_POSITIVE 307 124 89 80 45 tfor:TF0494|3end_conserved_hypothetical_protein_520906..521056_POSITIVE 299 135 95 73 27 tfor:TF2723|3end_30S_ribosomal_protein_S20_2898020..2898170_POSITIVE 296 140 95 85 41 tfor:TF1762|3end_hypothetical_protein_1899648..1899798_POSITIVE 294 119 76 119 83 tfor:TF3131|3end_uracil-DNA_glycosylase_3365248..3365398_POSITIVE 288 117 71 148 96 tfor:TF0678|3end_hypothetical_protein_721335..721485_POSITIVE 285 119 76 129 79 tfor:TF0017|3end_hypothetical_protein_11231..11381_POSITIVE 282 126 84 110 71 tfor:TF1311|3end_hypothetical_protein_1388558..1388708_POSITIVE 282 126 84 110 71 tfor:TF1310|3end_hypothetical_protein_1388335..1388485_POSITIVE 281 114 71 61 20 tfor:TF0249|3end_3-hydroxymyristoyl/3-hydroxydecanoyl-(acyl_carrier_protein)_dehydratase_279256..279406_POSITIVE 280 210 165 143 98 tfor:TF2138|3end_conserved_hypothetical_protein_2315446..2315596_POSITIVE