Origin IGS: atatttcgttgatatgcaataaaaaaataattttcactatgtaaaaaagtcactgatgcgcatttcaaagatgataatgacagatagcggacaggttggagcaaatccgctcaaataccgtttctgttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaacagaaacggtatttgagcggatttgctccaacctgtccgctatctgtcattatcatctttgaaatgcgcatcagtgacttttttacatagtgaaaattatttttttattgcatatcaacgaaatat Mask Tandem Repeat Region ================================================ atatttcgttgatatgcaataaaaaaataattttcactatgtaaaaaagtcactgatgcgcatttcaaagatgataatgacagatagcggacaggttggagcaaatccgctcaaataccgtttctgttta Find is-nt database================================================ Query_seq: TF1229:TF1230|TF1229:TF1230:hypothetical protein:hypothetical protein:->->:1314915..1315044 130 atatttcgttgatatgcaataaaaaaataattttcactatgtaaaaaagtcactgatgcgcatttcaaagatgataatgacagatagcggacaggttggagcaaatccgctcaaataccgtttctgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1229:TF1230|TF1229:TF1230:hypothetical protein:hypothetical protein:->->:1314915..1315044 130 atatttcgttgatatgcaataaaaaaataattttcactatgtaaaaaagtcactgatgcgcatttcaaagatgataatgacagatagcggacaggttggagcaaatccgctcaaataccgtttctgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1229:TF1230|TF1229:TF1230:hypothetical protein:hypothetical protein:->->:1314915..1315044 130 atatttcgttgatatgcaataaaaaaataattttcactatgtaaaaaagtcactgatgcgcatttcaaagatgataatgacagatagcggacaggttggagcaaatccgctcaaataccgtttctgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atatttcgttgatatgcaataaaaaaataattttcactatgtaaaaaagtcactgatgcgcatttcaaagatgataatgacagatagcggacaggttggagcaaatccgctcaaataccgtttctgttta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: - Start: 3 End: 104 .. N R Q Y A I F F Y N E S H L F T V S A C K L S S L S L Y R V P Q L M .......................... atatttcgttgatatgcaataaaaaaataattttcactatgtaaaaaagtcactgatgcgcatttcaaagatgataatgacagatagcggacaggttggagcaaatccgctcaaataccgtttctgttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atatttcgttgatatgcaataaaaaaataattttcactatgtaaaaaagtcactgatgcgcatttcaaagatgataatgacagatagcggacaggttggagcaaatccgctcaaataccgtttctgttta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 73 HP_score: -4.5 Tail_Score: -4.96435 Start: 31 End: 47 Full_Region: tgcgcatcagtgact tttttac ata gtgaaaa ttatttttttattgc ..............................tttttacatagtgaaaa................................................................................... Find igs database================================================ Query_seq: TF1229:TF1230|TF1229:TF1230:hypothetical protein:hypothetical protein:->->:1314915..1315044 130 atatttcgttgatatgcaataaaaaaataattttcactatgtaaaaaagtcactgatgcgcatttcaaagatgataatgacagatagcggacaggttggagcaaatccgctcaaataccgtttctgttta Intra-Species Hit: Count: 1 Min: 1 Max: 130 Len: 130 Subject: tfor_TF1229_TF1230|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1314915,1315044]|130 HSP 1 e-value: 1.0E-55 bit: 216.0 Len: 130 Query Start:1 Query End:130 Subject Strand: POSITIVE Subject Start: 1 Subject End: 130 atatttcgttgatatgcaatnnnnnnntaattttcactatgtaaaaaagtcactgatgcgcatttcaaagatgataatgacagatagcggacaggttggagcaaatccgctcaaataccgtttctgttta atatttcgttgatatgcaataaaaaaataattttcactatgtaaaaaagtcactgatgcgcatttcaaagatgataatgacagatagcggacaggttggagcaaatccgctcaaataccgtttctgttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAUUUCGUUGAUAUGCAAUAAAAAAAUAAUUUUCACUAUGUAAAAAAGUCACUGAUGCGCAUUUCAAAGAUGAUAAUGACAGAUAGCGGACAGGUUGGAGCAAAUCCGCUCAAAUACCGUUUCUGUUUA .((((((.((((.((((.............(((((.....).))))..(((...)))..)))).)))).)).))))..((((((.(((((....(((.((((......)))).))).))))))))))).. (-25.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACAGAAACGGUAUUUGAGCGGAUUUGCUCCAACCUGUCCGCUAUCUGUCAUUAUCAUCUUUGAAAUGCGCAUCAGUGACUUUUUUACAUAGUGAAAAUUAUUUUUUUAUUGCAUAUCAACGAAAUAU ...(((((...........(((((((..(......)..))))))).)))))........((.((((.((((......((((.....))))..((((((((.....)))))))))))).)))).))..... (-24.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 338 120 75 145 92 tfor:TF0142|5end_subtilisin-like_serine_proteinase_149740..149970_POSITIVE 335 119 79 49 1 tfor:TF2884|5end_conserved_hypothetical_protein_3075057..3075287_POSITIVE 327 127 86 114 74 tfor:TF1900|5end_conserved_hypothetical_protein_2053867..2054097_POSITIVE 319 120 71 222 170 tfor:TF1284|5end_glyceraldehyde_3-phosphate_dehydrogenase_1367939..1368169_POSITIVE 319 126 83 46 2 tfor:TF1240|5end_conserved_hypothetical_protein_1324304..1324534_POSITIVE 309 110 63 64 19 tfor:TF1464|5end_conserved_hypothetical_protein_1553467..1553697_POSITIVE 309 112 76 207 174 tfor:TF0542|5end_hypothetical_protein_571918..572148_POSITIVE 299 110 65 133 81 tfor:TF0657|5end_conserved_hypothetical_protein_702893..703123_POSITIVE 297 126 87 93 54 tfor:TF2911|5end_hypothetical_protein_3105871..3106101_POSITIVE 292 120 90 219 185 tfor:TF3112|5end_Na+/H+_anitporter_3346775..3347005_POSITIVE 292 120 90 45 11 tfor:TF3111|5end_hypothetical_protein_3346601..3346831_POSITIVE 291 120 71 66 18 tfor:TF3044|5end_two-component_system_sensor_kinase_3272691..3272921_POSITIVE 290 98 53 68 35 tfor:TF0947|5end_extracellular_protease_990627..990857_POSITIVE 289 129 86 88 51 tfor:TF2520|5end_conserved_hypothetical_protein_2698917..2699147_POSITIVE 288 98 52 82 36 tfor:TF2167|5end_hypothetical_protein_2342586..2342816_POSITIVE 288 98 52 82 36 tfor:TF2162|5end_thermolysin_precursor_2338669..2338899_POSITIVE 288 98 52 82 36 tfor:TF0367|5end_eukaryotic-like__metalloproteinase_384007..384237_POSITIVE 288 98 52 82 36 tfor:TF0364|5end_ysyl_endopeptidase_380395..380625_POSITIVE 288 98 52 82 36 tfor:TF0351|5end_hypothetical_protein_370922..371152_POSITIVE 284 130 83 89 29 tfor:TF0396|5end_conserved_hypothetical_protein_417018..417248_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 318 111 75 140 102 tfor:TF0141|3end_exodeoxyribonuclease_VII,_large_subunit_149830..149980_POSITIVE 309 112 76 67 34 tfor:TF0541|3end_conserved_hypothetical_protein_571858..572008_POSITIVE 299 110 65 133 81 tfor:TF0656|3end_acetyltransferase,_possible_GNAT_family_702973..703123_POSITIVE 297 126 87 98 59 tfor:TF2909|3end_GTP-binding_protein,_possible_GTP1/OBG_family_3105956..3106106_POSITIVE 290 98 53 59 26 tfor:TF0946|3end_iron(III)_ABC_transporter,_periplasmic_iron-binding_protein_990698..990848_POSITIVE 289 129 86 88 51 tfor:TF2519|3end_membrane_protein,_metallopeptidase_M23/M37_family_2698997..2699147_POSITIVE 283 121 81 120 75 tfor:TF0247|3end_conserved_hypothetical_protein_278348..278498_POSITIVE 282 119 88 41 2 tfor:TF2883|3end_peptidyl-prolyl_cis-trans_isomerase_3075129..3075279_POSITIVE 279 110 88 25 7 tfor:TF1462|3end_erythronate-4-phosphate_dehydrogenase_1553508..1553658_POSITIVE 277 41 1 36 1 tfor:TF2541|3end_transposase_2725232..2725382_POSITIVE 277 129 81 45 3 tfor:TF1800|3end_conserved_hypothetical_protein_1944251..1944401_POSITIVE 272 127 81 123 69 tfor:TF1832|3end_3-isopropylmalate_dehydratase,_small_subunit_1981502..1981652_POSITIVE 266 85 48 116 83 tfor:TF2877|3end_regulatory_protein_3067288..3067438_POSITIVE 263 125 81 57 14 tfor:TF0169|3end_possible_CRISPR-associated_protein_Cas1_179643..179793_POSITIVE 261 124 81 67 23 tfor:TF2642|3end_acetylornithine_deacetylase_2811827..2811977_POSITIVE 259 120 76 103 62 tfor:TF1761|3end_hypothetical_protein_1899579..1899729_POSITIVE 259 124 83 130 80 tfor:TF0207|3end_conserved_hypothetical_protein_233505..233655_POSITIVE 258 124 88 100 71 tfor:TF2654|3end_arginyl-tRNA_synthetase_2824559..2824709_POSITIVE 258 112 87 67 49 tfor:TF0537|3end_conserved_hypothetical_protein_566836..566986_POSITIVE 258 109 68 140 106 tfor:TF0218|3end_translation_initiation_factor_3_(IF-3)_243891..244041_POSITIVE