Origin IGS: gcctcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagagattcatcgcttacgctctgaatgacaaattaatggttaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattgataattaaccattgtcgtacattcgcatgtccattactaacggtaaatgccgaacagtgtatcgcgtaatttgggtttgtaaaaataattcattaaataatttggtttacaaaataaactacattatttttgcgatgtgaatcgattcaaaaaaaggaattatttagtaacaagtaaaaaaaacagtt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aactgttttttttacttgttactaaataattcctttttttgaatcgattcacatcgcaaaaataatgtagtttattttgtaaaccaaattatttaatgaattatttttacaaacccaaattacgcgatacactgttcggcatttaccgttagtaatggacatgcgaatgtacgacaatggttaattatcaatggttattttctaaatacatgattcggcgtgtacccggcataacaattaaccattaaccattaatttgtcattcagagcgtaagcgatgaatctcttttccgttacttttggcttgacccaaaagtaaccaaaaggtcaaggcttcgtgcgcttcacacgccttcgcgaggc Mask Tandem Repeat Region ================================================ gcctcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagagattcatcgcttacgctctgaatgacaaattaatggttaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattgataattaaccattgtcgtacattcgcatgtccattactaacggtaaatgccgaacagtgtatcgcgtaatttgggtttgtaaaaataattcattaaataatttggtttacaaaataaactacattatttttgcgatgtgaatcgattcaaaaaaaggaattatttagtaacaagtaaaaaaaacagtt Find is-nt database================================================ Query_seq: TF1187:TF1188|TF1187:TF1188:hypothetical protein:conserved hypothetical protein:->->:1269933..1270295 363 gcctcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagagattcatcgcttacgctctgaatgacaaattaatggttaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattgataattaaccattgtcgtacattcgcatgtccattactaacggtaaatgccgaacagtgtatcgcgtaatttgggtttgtaaaaataattcattaaataatttggtttacaaaataaactacattatttttgcgatgtgaatcgattcaaaaaaaggaattatttagtaacaagtaaaaaaaacagtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1187:TF1188|TF1187:TF1188:hypothetical protein:conserved hypothetical protein:->->:1269933..1270295 363 gcctcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagagattcatcgcttacgctctgaatgacaaattaatggttaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattgataattaaccattgtcgtacattcgcatgtccattactaacggtaaatgccgaacagtgtatcgcgtaatttgggtttgtaaaaataattcattaaataatttggtttacaaaataaactacattatttttgcgatgtgaatcgattcaaaaaaaggaattatttagtaacaagtaaaaaaaacagtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1187:TF1188|TF1187:TF1188:hypothetical protein:conserved hypothetical protein:->->:1269933..1270295 363 gcctcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagagattcatcgcttacgctctgaatgacaaattaatggttaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattgataattaaccattgtcgtacattcgcatgtccattactaacggtaaatgccgaacagtgtatcgcgtaatttgggtttgtaaaaataattcattaaataatttggtttacaaaataaactacattatttttgcgatgtgaatcgattcaaaaaaaggaattatttagtaacaagtaaaaaaaacagtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gcctcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagagattcatcgcttacgctctgaatgacaaattaatggttaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattgataattaaccattgtcgtacattcgcatgtccattactaacggtaaatgccgaacagtgtatcgcgtaatttgggtttgtaaaaataattcattaaataatttggtttacaaaataaactacattatttttgcgatgtgaatcgattcaaaaaaaggaattatttagtaacaagtaaaaaaaacagtt Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: + Start: 187 End: 312 .......................................................................................................................................................................................... M S Y I R M S I T N G K C R T V Y R V I W V C K N N S L N N L V Y K I N Y I I F A M ................................................... Protein_Len: 37 Strand: - Start: 154 End: 264 ......................................................................................................................................................... T N L F Y G N I I L W Q R V N A H G N S V T F A S C H I A Y N P N T F I M ................................................................................................... Protein_Len: 34 Strand: - Start: 1 End: 102 G R S P T H L A R L R S R K T V K P D L W F Y R F L S E D S V S Q M ..................................................................................................................................................................................................................................................................... gcctcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagagattcatcgcttacgctctgaatgacaaattaatggttaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattgataattaaccattgtcgtacattcgcatgtccattactaacggtaaatgccgaacagtgtatcgcgtaatttgggtttgtaaaaataattcattaaataatttggtttacaaaataaactacattatttttgcgatgtgaatcgattcaaaaaaaggaattatttagtaacaagtaaaaaaaacagtt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gcctcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagagattcatcgcttacgctctgaatgacaaattaatggttaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattgataattaaccattgtcgtacattcgcatgtccattactaacggtaaatgccgaacagtgtatcgcgtaatttgggtttgtaaaaataattcattaaataatttggtttacaaaataaactacattatttttgcgatgtgaatcgattcaaaaaaaggaattatttagtaacaagtaaaaaaaacagtt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 62 HP_score: -2.6 Tail_Score: -4.64766 Start: 273 End: 301 Full_Region: AAATAATTCATTAAA TAATTTGGTTTA CAAAA TAAACTACATTA TTTTTGCGATGTGAA ................................................................................................................................................................................................................................................................................TAATTTGGTTTACAAAATAAACTACATTA.............................................................. Find igs database================================================ Query_seq: TF1187:TF1188|TF1187:TF1188:hypothetical protein:conserved hypothetical protein:->->:1269933..1270295 363 gcctcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagagattcatcgcttacgctctgaatgacaaattaatggttaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattgataattaaccattgtcgtacattcgcatgtccattactaacggtaaatgccgaacagtgtatcgcgtaatttgggtttgtaaaaataattcattaaataatttggtttacaaaataaactacattatttttgcgatgtgaatcgattcaaaaaaaggaattatttagtaacaagtaaaaaaaacagtt Intra-Species Hit: Count: 15 Min: 1 Max: 350 Len: 350 Subject: tfor_TF1187_TF1188|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1269933,1270295]|363 HSP 1 e-value: 0.0 bit: 652.0 Len: 350 Query Start:1 Query End:350 Subject Strand: POSITIVE Subject Start: 1 Subject End: 350 gcctcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagagattcatcgcttacgctctgaatgacaaattaatggttaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattgataattaaccattgtcgtacattcgcatgtccattactaacggtaaatgccgaacagtgtatcgcgtaatttgggtttgtaaaaataattcattaaataatttggtttacaaaataaactacattatttttgcgatgtgaatcgattcnnnnnnnggaattatttagtaacaagt............. gcctcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagagattcatcgcttacgctctgaatgacaaattaatggttaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattgataattaaccattgtcgtacattcgcatgtccattactaacggtaaatgccgaacagtgtatcgcgtaatttgggtttgtaaaaataattcattaaataatttggtttacaaaataaactacattatttttgcgatgtgaatcgattcaaaaaaaggaattatttagtaacaagt............. Subject: tfor_TF1659_TF1660|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1775270,1775408]|139 HSP 1 e-value: 2.0E-53 bit: 210.0 Len: 122 Query Start:4 Query End:125 Subject Strand: POSITIVE Subject Start: 4 Subject End: 124 ...tcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagagattcatcgcttacgctctgaatgacaaattaatggttaatggtta.............................................................................................................................................................................................................................................. ...tcgcgaaggcgtgtgaagcgcacgaagccttgacctttgggttacttttgggtcaagccaaaagtaacggaaaagagattcatcgctt-cgctctgaatgataaattaatggttaatggtta.............................................................................................................................................................................................................................................. Subject: tfor_TF2500_TF2501|ABC transporter, ATP-binding protein:DNA mismatch repair protein|POSITIVE:POSITIVE|[2675363,2676419]|1057 HSP 1 e-value: 2.0E-44 bit: 180.0 Len: 127 Query Start:4 Query End:130 Subject Strand: POSITIVE Subject Start: 647 Subject End: 772 ...tcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagagattcatcgcttacgctctgaatgacaaattaatggttaatggttaattgt......................................................................................................................................................................................................................................... ...tcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtgacag-aaggagattcttcgcttacgctctgaatgacatattaatggttaattgtcaattgt......................................................................................................................................................................................................................................... Subject: tfor_TF0685_TF0686|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[731193,732338]|1146 HSP 1 e-value: 3.0E-43 bit: 176.0 Len: 129 Query Start:4 Query End:132 Subject Strand: NEGATIVE Subject Start: 153 Subject End: 280 ...tcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagagattcatcgcttacgctctgaatgacaaattaatggttaatggttaattgtta....................................................................................................................................................................................................................................... ...tcgcgaaggcgtgtgaagcgcacgaagccttgacctttgggttactttttggtcaagccaaaagtgacaaaaaagagattcttcgctt-cgctctgaatgacaaattaatggttaattgtcaattgtta....................................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-17 bit: 91.7 Len: 70 Query Start:76 Query End:145 Subject Strand: NEGATIVE Subject Start: 517 Subject End: 585 ...........................................................................aagagattcatcgcttacgctctgaatgacaaattaatggttaatggttaattgttatgccgggtacacg.......................................................................................................................................................................................................................... ...........................................................................aagagattcatcgctt-cgctctgaatgacaaattaatggtcaattgttaattgttatgcgtggtacacg.......................................................................................................................................................................................................................... Subject: tfor_TF1838_TF1839|tRNA/rRNA methyltransferase:conserved hypothetical protein|POSITIVE:POSITIVE|[1988806,1989486]|681 HSP 1 e-value: 2.0E-35 bit: 151.0 Len: 107 Query Start:75 Query End:181 Subject Strand: POSITIVE Subject Start: 148 Subject End: 261 ..........................................................................aaagagattcatcgcttacgctctgaatgacaaattaatggttaat-------ggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattgataatt...................................................................................................................................................................................... ..........................................................................aaagagattcttcgcttacgctctgaatgacaaattaattgttaattgtcaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattgacaatt...................................................................................................................................................................................... HSP 2 e-value: 2.0E-14 bit: 81.8 Len: 52 Query Start:26 Query End:77 Subject Strand: POSITIVE Subject Start: 384 Subject End: 436 .........................cgaagccttgaccttttggttactttt-gggtcaagccaaaagtaacggaaaa.............................................................................................................................................................................................................................................................................................. .........................cgaagccttgacctttgggttactttttgggtcaagccaaaagtaacggaaaa.............................................................................................................................................................................................................................................................................................. Subject: tfor_TF2922_TF2923|surface antigen BspA:hypothetical protein|POSITIVE:POSITIVE|[3121599,3122307]|709 HSP 1 e-value: 1.0E-33 bit: 145.0 Len: 73 Query Start:4 Query End:76 Subject Strand: NEGATIVE Subject Start: 151 Subject End: 223 ...tcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaa............................................................................................................................................................................................................................................................................................... ...tcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaa............................................................................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-33 bit: 145.0 Len: 115 Query Start:75 Query End:189 Subject Strand: NEGATIVE Subject Start: 346 Subject End: 453 ..........................................................................aaagagattcatcgcttacgctctgaatgacaaattaatggttaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattgataattaaccattg.............................................................................................................................................................................. ..........................................................................aaagagattcttcgcttacgctctgaatgacaaattaatggttaat-------tgttatgcgtggtacacgccgaatcatgtatttagaaaataaccattgacaattaaccattg.............................................................................................................................................................................. HSP 3 e-value: 1.0E-11 bit: 71.9 Len: 67 Query Start:74 Query End:140 Subject Strand: NEGATIVE Subject Start: 546 Subject End: 618 .........................................................................aaaagagattcatcgcttacgctctgaatgacaaattaatggtt-------aatggttaattgttatgccgggt............................................................................................................................................................................................................................... .........................................................................aaaagagattcatcgctt-cgcgctgaatgacaaattaatggttaattgtcaatggttaattgttatgccgggt............................................................................................................................................................................................................................... Subject: tfor_TF2745_TF2746|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2928008,2928135]|128 HSP 1 e-value: 2.0E-29 bit: 131.0 Len: 102 Query Start:11 Query End:112 Subject Strand: POSITIVE Subject Start: 29 Subject End: 128 ..........ggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagagattcatcgcttacgctctgaatgacaaattaa........................................................................................................................................................................................................................................................... ..........ggcgtgcgaagcgcacgaagccttgatcttttggttacttttgggtcaagccaaaagtgacag-aaagagattcttcgctt-cgctctgaatgacaaattaa........................................................................................................................................................................................................................................................... Subject: tfor_TF2996_TF2997|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[3211182,3211547]|366 HSP 1 e-value: 7.0E-29 bit: 129.0 Len: 73 Query Start:4 Query End:76 Subject Strand: NEGATIVE Subject Start: 291 Subject End: 363 ...tcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaa............................................................................................................................................................................................................................................................................................... ...tcgcgaaggcgtgtgaagcgcacgaagccttgacctttgggttacttttgggtcaagccaaaagtaacagaaa............................................................................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-8 bit: 61.9 Len: 47 Query Start:109 Query End:155 Subject Strand: NEGATIVE Subject Start: 218 Subject End: 264 ............................................................................................................ttaatggttaatggttaattgttatgccgggtacacgccgaatcatg................................................................................................................................................................................................................ ............................................................................................................ttaattgtcaatggttaattgttatgcgtggtacacgccgaatcatg................................................................................................................................................................................................................ Subject: tfor_TF2920_TF2921|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[3117231,3118637]|1407 HSP 1 e-value: 7.0E-29 bit: 129.0 Len: 73 Query Start:4 Query End:76 Subject Strand: NEGATIVE Subject Start: 207 Subject End: 279 ...tcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaa............................................................................................................................................................................................................................................................................................... ...tcgcgaaggcgtgtgaagcgcacgaagccttgacctttgggttacttttgggtcaagccaaaagtaacagaaa............................................................................................................................................................................................................................................................................................... HSP 2 e-value: 4.0E-15 bit: 83.8 Len: 46 Query Start:75 Query End:120 Subject Strand: NEGATIVE Subject Start: 398 Subject End: 443 ..........................................................................aaagagattcatcgcttacgctctgaatgacaaattaatggttaat................................................................................................................................................................................................................................................... ..........................................................................aaagagattcttcgcttacgctctgaatgacaaattaatggttaat................................................................................................................................................................................................................................................... HSP 3 e-value: 2.0E-7 bit: 58.0 Len: 33 Query Start:76 Query End:108 Subject Strand: NEGATIVE Subject Start: 729 Subject End: 761 ...........................................................................aagagattcatcgcttacgctctgaatgacaaa............................................................................................................................................................................................................................................................... ...........................................................................aagagattcttcgcttacgctctgaatgacaaa............................................................................................................................................................................................................................................................... Subject: tfor_TF2914_TF2915|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[3109704,3110561]|858 HSP 1 e-value: 3.0E-28 bit: 127.0 Len: 80 Query Start:1 Query End:80 Subject Strand: NEGATIVE Subject Start: 9 Subject End: 88 gcctcgcgaaggcgtgtgaagcgcacgaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagag........................................................................................................................................................................................................................................................................................... gcctcgcgaaggcgtgtgaagagcacgaagccttgactttttggttacttttgggtcaagccaaaagtgacagaaaagag........................................................................................................................................................................................................................................................................................... HSP 2 e-value: 6.0E-8 bit: 60.0 Len: 42 Query Start:79 Query End:120 Subject Strand: NEGATIVE Subject Start: 204 Subject End: 244 ..............................................................................agattcatcgcttacgctctgaatgacaaattaatggttaat................................................................................................................................................................................................................................................... ..............................................................................agattcttcgctt-cgctctgaatgacaaattaatggttaat................................................................................................................................................................................................................................................... Subject: tfor_TF2921_TF2922|hypothetical protein:surface antigen BspA|POSITIVE:POSITIVE|[3118851,3119261]|411 HSP 1 e-value: 7.0E-26 bit: 119.0 Len: 98 Query Start:79 Query End:176 Subject Strand: NEGATIVE Subject Start: 37 Subject End: 127 ..............................................................................agattcatcgcttacgctctgaatgacaaattaatggttaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattga........................................................................................................................................................................................... ..............................................................................agattcttcgcttacgctctgaatgacaaattaatggttaat-------tgttatgcgtggtacacgccgaatcatgtatttagaaaataaccattga........................................................................................................................................................................................... HSP 2 e-value: 4.0E-15 bit: 83.8 Len: 50 Query Start:76 Query End:125 Subject Strand: NEGATIVE Subject Start: 253 Subject End: 302 ...........................................................................aagagattcatcgcttacgctctgaatgacaaattaatggttaatggtta.............................................................................................................................................................................................................................................. ...........................................................................aagagattcttcgcttacgctctgaatgacaaattaatggttaattgtta.............................................................................................................................................................................................................................................. Subject: tfor_TF1846_TF1847|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1997076,1997528]|453 HSP 1 e-value: 3.0E-22 bit: 107.0 Len: 66 Query Start:111 Query End:176 Subject Strand: POSITIVE Subject Start: 87 Subject End: 152 ..............................................................................................................aatggttaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattga........................................................................................................................................................................................... ..............................................................................................................aatggttaatggttaatggttatgcgtggtacacgccgaatcatgtatttagaaaataaccattga........................................................................................................................................................................................... HSP 2 e-value: 7.0E-20 bit: 99.6 Len: 54 Query Start:27 Query End:80 Subject Strand: POSITIVE Subject Start: 1 Subject End: 54 ..........................gaagccttgaccttttggttacttttgggtcaagccaaaagtaacggaaaagag........................................................................................................................................................................................................................................................................................... ..........................gaagccttgaccttttggttacttttgggtcaagccaaaagtaacagaaaagag........................................................................................................................................................................................................................................................................................... HSP 3 e-value: 3.0E-6 bit: 54.0 Len: 27 Query Start:94 Query End:120 Subject Strand: POSITIVE Subject Start: 56 Subject End: 82 .............................................................................................gctctgaatgacaaattaatggttaat................................................................................................................................................................................................................................................... .............................................................................................gctctgaatgacaaattaatggttaat................................................................................................................................................................................................................................................... Subject: tfor_TF0467_TF0468|acetyltransferase-related protein:hypothetical protein|POSITIVE:POSITIVE|[486604,487068]|465 HSP 1 e-value: 7.0E-20 bit: 99.6 Len: 70 Query Start:181 Query End:250 Subject Strand: NEGATIVE Subject Start: 24 Subject End: 93 ....................................................................................................................................................................................taaccattgtcgtacattcgcatgtccattactaacggtaaatgccgaacagtgtatcgcgtaatttggg................................................................................................................. ....................................................................................................................................................................................taaccattgtcgtacattcgcacgtccattattaatggtaaacgccgaacaatgtatcgcgtaatttggg................................................................................................................. HSP 2 e-value: 4.0E-12 bit: 73.8 Len: 73 Query Start:117 Query End:189 Subject Strand: NEGATIVE Subject Start: 381 Subject End: 453 ....................................................................................................................taatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattgataattaaccattg.............................................................................................................................................................................. ....................................................................................................................taattgttaattgttatgcgtggtacacgccgaatcatgtacccagcaaataaccattgacaattaacaattg.............................................................................................................................................................................. Subject: tfor_TF2665_TF2667|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2842296,2842534]|239 HSP 1 e-value: 1.0E-18 bit: 95.6 Len: 68 Query Start:109 Query End:176 Subject Strand: NEGATIVE Subject Start: 158 Subject End: 225 ............................................................................................................ttaatggttaatggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataaccattga........................................................................................................................................................................................... ............................................................................................................ttaattgtcaatggttaattgttatgcgtggtacacgccgaatcatgtatttagaaaataacaattga........................................................................................................................................................................................... HSP 2 e-value: 9.0E-10 bit: 65.9 Len: 37 Query Start:1 Query End:37 Subject Strand: NEGATIVE Subject Start: 3 Subject End: 39 gcctcgcgaaggcgtgtgaagcgcacgaagccttgac...................................................................................................................................................................................................................................................................................................................................... gcctcgcgaaggtgtgtgaagcgcacgaagccttgac...................................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF2816_TF2817|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[3007245,3007497]|253 HSP 1 e-value: 4.0E-15 bit: 83.8 Len: 101 Query Start:72 Query End:172 Subject Strand: POSITIVE Subject Start: 147 Subject End: 253 .......................................................................ggaaaagagattcatcgcttacgctctgaatgacaaattaatggttaat-------ggttaattgttatgccgggtacacgccgaatcatgtatttagaaaataacca............................................................................................................................................................................................... .......................................................................ggaaaggagattcttcgcttt-gctctgaatgacaaattaattgttaattgtcaatggtcaattattatgcgtggtacacgccgaatcatgtatttagaaaataacca............................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCCUCGCGAAGGCGUGUGAAGCGCACGAAGCCUUGACCUUUUGGUUACUUUUGGGUCAAGCCAAAAGUAACGGAAAAGAGAUUCAUCGCUUACGCUCUGAAUGACAAAUUAAUGGUUAAUGGUUAAUUGUUAUGCCGGGUACACGCCGAAUCAUGUAUUUAGAAAAUAACCAUUGAUAAUUAACCAUUGUCGUACAUUCGCAUGUCCAUUACUAACGGUAAAUGCCGAACAGUGUAUCGCGUAAUUUGGGUUUGUAAAAAUAAUUCAUUAAAUAAUUUGGUUUACAAAAUAAACUACAUUAUUUUUGCGAUGUGAAUCGAUUCAAAAAAAGGAAUUAUUUAGUAACAAGU (((((((((.(((((((((.(((....((((..(((.(((((.(((((((((((......)))))))))))....)))))..)))..)))).)))..((.((((((.....(((((((((.((((((.(((((.(..((((((.(......).))))))..)...))))).)))))).))))))))))))))).)).)))))))))...((((..((((....))))...))))..)))))......))))((((..(((((((((...(((((((.(((((((.....))))))).)))))))...((((....))))............)))))))))....)))).. (-98.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACUUGUUACUAAAUAAUUCCUUUUUUUGAAUCGAUUCACAUCGCAAAAAUAAUGUAGUUUAUUUUGUAAACCAAAUUAUUUAAUGAAUUAUUUUUACAAACCCAAAUUACGCGAUACACUGUUCGGCAUUUACCGUUAGUAAUGGACAUGCGAAUGUACGACAAUGGUUAAUUAUCAAUGGUUAUUUUCUAAAUACAUGAUUCGGCGUGUACCCGGCAUAACAAUUAACCAUUAACCAUUAAUUUGUCAUUCAGAGCGUAAGCGAUGAAUCUCUUUUCCGUUACUUUUGGCUUGACCCAAAAGUAACCAAAAGGUCAAGGCUUCGUGCGCUUCACACGCCUUCGCGAGGC .((((.............((((((........((((((..((((.........((((((((((..((((......))))..))))))))))...............((((((.....((((..(((......))).))))...........(((((.((((.((((((((.......(((((((.........((((((......))))))...(......)...)))))))))))))))...)))))))))...))))))))))))))))........(((((((((((......))))))))))).)))))).))))((((((((.((.......))...)))))))) (-84.25) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 993 60 11 151 102 tfor:TF1578|5end_hypothetical_protein_1686187..1686417_POSITIVE 993 60 11 64 15 tfor:TF0685|5end_hypothetical_protein_730852..731082_POSITIVE 969 50 1 74 25 tfor:TF0469|5end_hypothetical_protein_486960..487190_POSITIVE 941 60 11 151 102 tfor:TF2997|5end_hypothetical_protein_3211408..3211638_POSITIVE 941 60 11 125 76 tfor:TF2996|5end_hypothetical_protein_3210802..3211032_POSITIVE 941 60 11 135 86 tfor:TF1588|5end_hypothetical_protein_1702268..1702498_POSITIVE 922 60 13 53 6 tfor:TF0468|5end_hypothetical_protein_486929..487159_POSITIVE 909 60 11 135 83 tfor:TF1168|5end_hypothetical_protein_1248755..1248985_POSITIVE 814 240 191 156 102 tfor:TF2640|5end_hypothetical_protein_2809106..2809336_POSITIVE 814 240 191 70 16 tfor:TF2639|5end_hypothetical_protein_2809020..2809250_POSITIVE 748 189 141 107 59 tfor:TF2666|5end_hypothetical_protein_2841818..2842048_POSITIVE 722 170 121 167 118 tfor:TF2667|5end_hypothetical_protein_2842395..2842625_POSITIVE 722 170 121 88 39 tfor:TF2664|5end_hypothetical_protein_2841399..2841629_POSITIVE 721 180 131 98 49 tfor:TF2670|5end_hypothetical_protein_2842779..2843009_POSITIVE 693 230 181 112 63 tfor:TF3003|5end_hypothetical_protein_3220382..3220612_POSITIVE 675 250 202 59 11 tfor:TF2025|5end_hypothetical_protein_2181933..2182163_POSITIVE 673 120 71 142 95 tfor:TF2653|5end_hypothetical_protein_2822093..2822323_POSITIVE 672 249 202 231 184 tfor:TF1274|5end_hypothetical_protein_1357621..1357851_POSITIVE 670 180 131 189 140 tfor:TF0470|5end_hypothetical_protein_487253..487483_POSITIVE 666 130 81 220 171 tfor:TF1579|5end_hypothetical_protein_1686889..1687119_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 993 60 11 118 69 tfor:TF1577|3end_hypothetical_protein_1686234..1686384_POSITIVE 941 60 11 92 43 tfor:TF2994|3end_hypothetical_protein_3210849..3210999_POSITIVE 894 50 1 52 3 tfor:TF2914|3end_hypothetical_protein_3109643..3109793_POSITIVE 871 80 31 52 3 tfor:TF2639|3end_hypothetical_protein_2809333..2809483_POSITIVE 855 50 1 129 80 tfor:TF2668|3end_hypothetical_protein_2842733..2842883_POSITIVE 855 50 1 101 52 tfor:TF2667|3end_hypothetical_protein_2842705..2842855_POSITIVE 855 50 1 101 52 tfor:TF2665|3end_hypothetical_protein_2842235..2842385_POSITIVE 855 50 1 129 80 tfor:TF2666|3end_hypothetical_protein_2842263..2842413_POSITIVE 855 50 1 101 52 tfor:TF2664|3end_hypothetical_protein_2841730..2841880_POSITIVE 766 240 191 57 8 tfor:TF0467|3end_acetyltransferase-related_protein_486543..486693_POSITIVE 740 130 81 88 39 tfor:TF1576|3end_hypothetical_protein_1686135..1686285_POSITIVE 739 120 72 57 9 tfor:TF1578|3end_hypothetical_protein_1686416..1686566_POSITIVE 725 189 141 53 5 tfor:TF2438|3end_hypothetical_protein_2616157..2616307_POSITIVE 705 250 202 95 47 tfor:TF2047|3end_hypothetical_protein_2210298..2210448_POSITIVE 698 170 121 142 93 tfor:TF2997|3end_hypothetical_protein_3211664..3211814_POSITIVE 675 176 131 54 9 tfor:TF2921|3end_hypothetical_protein_3118790..3118940_POSITIVE 673 120 71 91 44 tfor:TF2652|3end_hypothetical_protein_2822122..2822272_POSITIVE 670 180 131 84 35 tfor:TF0469|3end_hypothetical_protein_487228..487378_POSITIVE 630 255 218 38 1 tfor:TF1540|3end_possible_ATPase_1645987..1646137_POSITIVE 621 189 141 53 5 tfor:TF0468|3end_hypothetical_protein_487188..487338_POSITIVE