Origin IGS: taaatatcctccccccaacaaagagcgcacttctccggcttttcgcatcccgtaaagagaaaacggccttcgcttatcggaagattccccaaaatcgtccgagcaaaaaatcattttaaccgttcattctaatctttttacgaatccaactgtcatcaaattttctttttatcccggattccgcaatagaagaaataagaatgaatttaattcaggccttcagcccttgtgctccgtgcgggctaaccccttcggggcgttgaggggttgggttgttgaaatgtttagttgttgaaatgtttagtcgttgaggtgtttagccgttgaggtatttagttgttgaaatgtttagttgttgatctgtttactgaccactgtaaaagccctgaaagggcgcattaattcagcccaatgcgaagcgttggggaaaaaggcatcgcatctcggcgaagggctgttaattcaattgttaattgtca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgacaattaacaattgaattaacagcccttcgccgagatgcgatgcctttttccccaacgcttcgcattgggctgaattaatgcgccctttcagggcttttacagtggtcagtaaacagatcaacaactaaacatttcaacaactaaatacctcaacggctaaacacctcaacgactaaacatttcaacaactaaacatttcaacaacccaacccctcaacgccccgaaggggttagcccgcacggagcacaagggctgaaggcctgaattaaattcattcttatttcttctattgcggaatccgggataaaaagaaaatttgatgacagttggattcgtaaaaagattagaatgaacggttaaaatgattttttgctcggacgattttggggaatcttccgataagcgaaggccgttttctctttacgggatgcgaaaagccggagaagtgcgctctttgttggggggaggatattta Mask Tandem Repeat Region ================================================ taaatatcctccccccaacaaagagcgcacttctccggcttttcgcatcccgtaaagagaaaacggccttcgcttatcggaagattccccaaaatcgtccgagcaaaaaatcattttaaccgttcattctaatctttttacgaatccaactgtcatcaaattttctttttatcccggattccgcaatagaagaaataagaatgaatttaattcaggccttcagcccttgtgctccgtgcgggctaaccccttcggggcgttgaggggttggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntctgtttactgaccactgtaaaagccctgaaagggcgcattaattcagcccaatgcgaagcgttggggaaaaaggcatcgcatctcggcgaagggctgttaattcaattgttaattgtca Find is-nt database================================================ Query_seq: TF1181:TF1183|TF1181:TF1183:methylated-DNA--[protein]-cysteine S-methyltransferase:hypothetical protein:->->:1268052..1268530 479 taaatatcctccccccaacaaagagcgcacttctccggcttttcgcatcccgtaaagagaaaacggccttcgcttatcggaagattccccaaaatcgtccgagcaaaaaatcattttaaccgttcattctaatctttttacgaatccaactgtcatcaaattttctttttatcccggattccgcaatagaagaaataagaatgaatttaattcaggccttcagcccttgtgctccgtgcgggctaaccccttcggggcgttgaggggttgggttgttgaaatgtttagttgttgaaatgtttagtcgttgaggtgtttagccgttgaggtatttagttgttgaaatgtttagttgttgatctgtttactgaccactgtaaaagccctgaaagggcgcattaattcagcccaatgcgaagcgttggggaaaaaggcatcgcatctcggcgaagggctgttaattcaattgttaattgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1181:TF1183|TF1181:TF1183:methylated-DNA--[protein]-cysteine S-methyltransferase:hypothetical protein:->->:1268052..1268530 479 taaatatcctccccccaacaaagagcgcacttctccggcttttcgcatcccgtaaagagaaaacggccttcgcttatcggaagattccccaaaatcgtccgagcaaaaaatcattttaaccgttcattctaatctttttacgaatccaactgtcatcaaattttctttttatcccggattccgcaatagaagaaataagaatgaatttaattcaggccttcagcccttgtgctccgtgcgggctaaccccttcggggcgttgaggggttgggttgttgaaatgtttagttgttgaaatgtttagtcgttgaggtgtttagccgttgaggtatttagttgttgaaatgtttagttgttgatctgtttactgaccactgtaaaagccctgaaagggcgcattaattcagcccaatgcgaagcgttggggaaaaaggcatcgcatctcggcgaagggctgttaattcaattgttaattgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1181:TF1183|TF1181:TF1183:methylated-DNA--[protein]-cysteine S-methyltransferase:hypothetical protein:->->:1268052..1268530 479 taaatatcctccccccaacaaagagcgcacttctccggcttttcgcatcccgtaaagagaaaacggccttcgcttatcggaagattccccaaaatcgtccgagcaaaaaatcattttaaccgttcattctaatctttttacgaatccaactgtcatcaaattttctttttatcccggattccgcaatagaagaaataagaatgaatttaattcaggccttcagcccttgtgctccgtgcgggctaaccccttcggggcgttgaggggttgggttgttgaaatgtttagttgttgaaatgtttagtcgttgaggtgtttagccgttgaggtatttagttgttgaaatgtttagttgttgatctgtttactgaccactgtaaaagccctgaaagggcgcattaattcagcccaatgcgaagcgttggggaaaaaggcatcgcatctcggcgaagggctgttaattcaattgttaattgtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaatatcctccccccaacaaagagcgcacttctccggcttttcgcatcccgtaaagagaaaacggccttcgcttatcggaagattccccaaaatcgtccgagcaaaaaatcattttaaccgttcattctaatctttttacgaatccaactgtcatcaaattttctttttatcccggattccgcaatagaagaaataagaatgaatttaattcaggccttcagcccttgtgctccgtgcgggctaaccccttcggggcgttgaggggttgggttgttgaaatgtttagttgttgaaatgtttagtcgttgaggtgtttagccgttgaggtatttagttgttgaaatgtttagttgttgatctgtttactgaccactgtaaaagccctgaaagggcgcattaattcagcccaatgcgaagcgttggggaaaaaggcatcgcatctcggcgaagggctgttaattcaattgttaattgtca Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 6 End: 116 ..... M L P P T K S A L L R L F A S R K E K T A F A Y R K I P Q N R P S K K S F ........................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 42 Strand: + Start: 4 End: 129 ... M S S P Q Q R A H F S G F S H P V K R K R P S L I G R F P K I V R A K N H F N R S F .............................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 45 Strand: + Start: 126 End: 260 ............................................................................................................................. M L I F L R I Q L S S N F L F I P D S A I E E I R M N L I Q A F S P C A P C G L T P S G R ........................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 110 End: 292 ............................................................................................................. M I L T V H S N L F T N P T V I K F S F Y P G F R N R R N K N E F N S G L Q P L C S V R A N P F G A L R G W V V E M F S ........................................................................................................................................................................................... Protein_Len: 42 Strand: + Start: 353 End: 478 ................................................................................................................................................................................................................................................................................................................................................................ M L I C L L T T V K A L K G R I N S A Q C E A L G K K A S H L G E G L L I Q L L I V . Protein_Len: 37 Strand: - Start: 352 End: 462 ............................................................................................................................................................................................................................................................................................................................................................... N N I Q K S V V T F A R F P R M L E A W H S A N P F F A D C R P S P S N M ................. Protein_Len: 31 Strand: - Start: 119 End: 211 ...................................................................................................................... G N M R I K K R I W S D D F K R K I G S E A I S S I L I F K M ............................................................................................................................................................................................................................................................................ Protein_Len: 31 Strand: - Start: 3 End: 95 .. I D E G W C L A C K E P K E C G T F L F R G E S I P L N G L M ................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 38 Strand: - Start: 1 End: 114 L Y G G G L L S R V E G A K R M G Y L S F P R R K D S S E G F D D S C F I M ............................................................................................................................................................................................................................................................................................................................................................................. taaatatcctccccccaacaaagagcgcacttctccggcttttcgcatcccgtaaagagaaaacggccttcgcttatcggaagattccccaaaatcgtccgagcaaaaaatcattttaaccgttcattctaatctttttacgaatccaactgtcatcaaattttctttttatcccggattccgcaatagaagaaataagaatgaatttaattcaggccttcagcccttgtgctccgtgcgggctaaccccttcggggcgttgaggggttgggttgttgaaatgtttagttgttgaaatgtttagtcgttgaggtgtttagccgttgaggtatttagttgttgaaatgtttagttgttgatctgtttactgaccactgtaaaagccctgaaagggcgcattaattcagcccaatgcgaagcgttggggaaaaaggcatcgcatctcggcgaagggctgttaattcaattgttaattgtca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaatatcctccccccaacaaagagcgcacttctccggcttttcgcatcccgtaaagagaaaacggccttcgcttatcggaagattccccaaaatcgtccgagcaaaaaatcattttaaccgttcattctaatctttttacgaatccaactgtcatcaaattttctttttatcccggattccgcaatagaagaaataagaatgaatttaattcaggccttcagcccttgtgctccgtgcgggctaaccccttcggggcgttgaggggttgggttgttgaaatgtttagttgttgaaatgtttagtcgttgaggtgtttagccgttgaggtatttagttgttgaaatgtttagttgttgatctgtttactgaccactgtaaaagccctgaaagggcgcattaattcagcccaatgcgaagcgttggggaaaaaggcatcgcatctcggcgaagggctgttaattcaattgttaattgtca Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 82 HP_score: -9.1 Tail_Score: -4.15565 Start: 383 End: 395 Full_Region: gggctgaattaatgc gccct ttc agggc ttttacagtggtcag ..............................................................................................................................................................................................................................................................................................................................................................................................gccctttcagggc.................................................................................... Find igs database================================================ Query_seq: TF1181:TF1183|TF1181:TF1183:methylated-DNA--[protein]-cysteine S-methyltransferase:hypothetical protein:->->:1268052..1268530 479 taaatatcctccccccaacaaagagcgcacttctccggcttttcgcatcccgtaaagagaaaacggccttcgcttatcggaagattccccaaaatcgtccgagcaaaaaatcattttaaccgttcattctaatctttttacgaatccaactgtcatcaaattttctttttatcccggattccgcaatagaagaaataagaatgaatttaattcaggccttcagcccttgtgctccgtgcgggctaaccccttcggggcgttgaggggttgggttgttgaaatgtttagttgttgaaatgtttagtcgttgaggtgtttagccgttgaggtatttagttgttgaaatgtttagttgttgatctgtttactgaccactgtaaaagccctgaaagggcgcattaattcagcccaatgcgaagcgttggggaaaaaggcatcgcatctcggcgaagggctgttaattcaattgttaattgtca Intra-Species Hit: Count: 13 Min: 1 Max: 479 Len: 479 Subject: tfor_TF1181_TF1183|methylated-DNA--[protein]-cysteine S-methyltransferase:hypothetical protein|POSITIVE:POSITIVE|[1268052,1268530]|479 HSP 1 e-value: 0.0 bit: 950.0 Len: 479 Query Start:1 Query End:479 Subject Strand: POSITIVE Subject Start: 1 Subject End: 479 taaatatcctccccccaacaaagagcgcacttctccggcttttcgcatcccgtaaagagaaaacggccttcgcttatcggaagattccccaaaatcgtccgagcaaaaaatcattttaaccgttcattctaatctttttacgaatccaactgtcatcaaattttctttttatcccggattccgcaatagaagaaataagaatgaatttaattcaggccttcagcccttgtgctccgtgcgggctaaccccttcggggcgttgaggggttgggttgttgaaatgtttagttgttgaaatgtttagtcgttgaggtgtttagccgttgaggtatttagttgttgaaatgtttagttgttgatctgtttactgaccactgtaaaagccctgaaagggcgcattaattcagcccaatgcgaagcgttggggaaaaaggcatcgcatctcggcgaagggctgttaattcaattgttaattgtca taaatatcctccccccaacaaagagcgcacttctccggcttttcgcatcccgtaaagagaaaacggccttcgcttatcggaagattccccaaaatcgtccgagcaaaaaatcattttaaccgttcattctaatctttttacgaatccaactgtcatcaaattttctttttatcccggattccgcaatagaagaaataagaatgaatttaattcaggccttcagcccttgtgctccgtgcgggctaaccccttcggggcgttgaggggttgggttgttgaaatgtttagttgttgaaatgtttagtcgttgaggtgtttagccgttgaggtatttagttgttgaaatgtttagttgttgatctgtttactgaccactgtaaaagccctgaaagggcgcattaattcagcccaatgcgaagcgttggggaaaaaggcatcgcatctcggcgaagggctgttaattcaattgttaattgtca Subject: tfor_TF2640_TF2641|hypothetical protein:riboflavin kinase/FAD synthase|POSITIVE:POSITIVE|[2809594,2809820]|227 HSP 1 e-value: 7.0E-42 bit: 172.0 Len: 115 Query Start:343 Query End:457 Subject Strand: NEGATIVE Subject Start: 104 Subject End: 218 ......................................................................................................................................................................................................................................................................................................................................................aaatgtttagttgttgatctgtttactgaccactgtaaaagccctgaaagggcgcattaattcagcccaatgcgaagcgttggggaaaaaggcatcgcatctcggcgaagggctg...................... ......................................................................................................................................................................................................................................................................................................................................................aaatgtttagttgttgaattgtttactgaccgctgtaaaagccctgaaagggcgcattaattcagtccaatgcgaagcgttggggaaaaaagcatcgcatctcagcggagggctg...................... Subject: tfor_TF0685_TF0686|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[731193,732338]|1146 HSP 1 e-value: 9.0E-26 bit: 119.0 Len: 145 Query Start:314 Query End:458 Subject Strand: NEGATIVE Subject Start: 778 Subject End: 932 .........................................................................................................................................................................................................................................................................................................................tgtttagccgttgaggtatttagttgttgaaatgtttagttgttgatctgtttactgaccactgtaaaagccctgaaagggcgcattaattcagcccaat----------gcgaagcgttggggaaaaaggcatcgcatctcggcgaagggctgt..................... .........................................................................................................................................................................................................................................................................................................................tgtttagttgttgagatgttgagttgtttaattgttgagttgttgatttgtttgctgaccgttgtaacagccctgaaagggcgcattaattcagcccaatgtggagcgcagcgaagcgttgggaaaaaaggcatcgcatctcggcggagggctgt..................... HSP 2 e-value: 8.0E-14 bit: 79.8 Len: 44 Query Start:173 Query End:216 Subject Strand: NEGATIVE Subject Start: 949 Subject End: 992 ............................................................................................................................................................................cccggattccgcaatagaagaaataagaatgaatttaattcagg....................................................................................................................................................................................................................................................................... ............................................................................................................................................................................cccggattccgcgatagaagaaataagaatgaatttaattcagg....................................................................................................................................................................................................................................................................... Subject: tfor_TF2047_TF2048|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2210359,2210782]|424 HSP 1 e-value: 6.0E-24 bit: 113.0 Len: 77 Query Start:382 Query End:458 Subject Strand: NEGATIVE Subject Start: 76 Subject End: 152 .............................................................................................................................................................................................................................................................................................................................................................................................agccctgaaagggcgcattaattcagcccaatgcgaagcgttggggaaaaaggcatcgcatctcggcgaagggctgt..................... .............................................................................................................................................................................................................................................................................................................................................................................................agccctgaaagggcgagataattcagcccaatgcgaagcgttggggaaaaaggcatcgcatcccggcggagggctgt..................... HSP 2 e-value: 9.0E-20 bit: 99.6 Len: 62 Query Start:166 Query End:227 Subject Strand: NEGATIVE Subject Start: 337 Subject End: 398 .....................................................................................................................................................................tttttatcccggattccgcaatagaagaaataagaatgaatttaattcaggccttcagccct............................................................................................................................................................................................................................................................ .....................................................................................................................................................................tttttaacccagattccgcaatagaagaaataagaatgaatttaattcaggctttcagccct............................................................................................................................................................................................................................................................ Subject: tfor_TF0029_TF0030|hypothetical protein:N-acetylneuraminate lyase|POSITIVE:POSITIVE|[25123,25909]|787 HSP 1 e-value: 6.0E-24 bit: 113.0 Len: 84 Query Start:375 Query End:458 Subject Strand: POSITIVE Subject Start: 379 Subject End: 463 ......................................................................................................................................................................................................................................................................................................................................................................................ctgtaaaagccctgaaagggcgcattaattcagcccaatgcgaagcgttgggg-aaaaaggcatcgcatctcggcgaagggctgt..................... ......................................................................................................................................................................................................................................................................................................................................................................................ctgtaaaagccctgaaagggcataataattcagcccaatgcgaagcgttggggaaaaaaagcatcgcatctcggcggagggctgt..................... HSP 2 e-value: 3.0E-16 bit: 87.7 Len: 52 Query Start:177 Query End:228 Subject Strand: POSITIVE Subject Start: 18 Subject End: 69 ................................................................................................................................................................................gattccgcaatagaagaaataagaatgaatttaattcaggccttcagccctt........................................................................................................................................................................................................................................................... ................................................................................................................................................................................gattccgcaatagaaccaataagaatgaatttaattcaggccttcagccctt........................................................................................................................................................................................................................................................... Subject: tfor_TF2920_TF2921|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[3117231,3118637]|1407 HSP 1 e-value: 1.0E-21 bit: 105.0 Len: 85 Query Start:337 Query End:421 Subject Strand: NEGATIVE Subject Start: 888 Subject End: 972 ................................................................................................................................................................................................................................................................................................................................................ttgttgaaatgtttagttgttgatctgtttactgaccactgtaaaagccctgaaagggcgcattaattcagcccaatgcgaagcg.......................................................... ................................................................................................................................................................................................................................................................................................................................................ttgttgatctgtttagttgttgatctgtttgctgaccgttgtaagagccctgaaagggcgtattaattcagcccaatgcgcagcg.......................................................... HSP 2 e-value: 1.0E-6 bit: 56.0 Len: 40 Query Start:375 Query End:414 Subject Strand: NEGATIVE Subject Start: 572 Subject End: 611 ......................................................................................................................................................................................................................................................................................................................................................................................ctgtaaaagccctgaaagggcgcattaattcagcccaatg................................................................. ......................................................................................................................................................................................................................................................................................................................................................................................ctgtaacagccctgaaagggcgcgttaattcagtccaatg................................................................. HSP 3 e-value: 1.0E-6 bit: 56.0 Len: 52 Query Start:219 Query End:270 Subject Strand: NEGATIVE Subject Start: 983 Subject End: 1034 ..........................................................................................................................................................................................................................ttcagcccttgtgctccgtgcgggctaaccccttcggggcgttgaggggttg................................................................................................................................................................................................................. ..........................................................................................................................................................................................................................ttcagcccttgggcctcgggcgagctaaccccttcggggcgttgaggtgttg................................................................................................................................................................................................................. Subject: tfor_TF1762_TF1763|hypothetical protein:K+-dependent Na+/Ca+ exchanger related-protein|POSITIVE:POSITIVE|[1899709,1900545]|837 HSP 1 e-value: 2.0E-20 bit: 101.0 Len: 55 Query Start:173 Query End:227 Subject Strand: NEGATIVE Subject Start: 302 Subject End: 356 ............................................................................................................................................................................cccggattccgcaatagaagaaataagaatgaatttaattcaggccttcagccct............................................................................................................................................................................................................................................................ ............................................................................................................................................................................cccggattccgcaatagaagaaataaaaatgaatttaattcaggccttcagccct............................................................................................................................................................................................................................................................ HSP 2 e-value: 5.0E-12 bit: 73.8 Len: 77 Query Start:219 Query End:295 Subject Strand: NEGATIVE Subject Start: 164 Subject End: 240 ..........................................................................................................................................................................................................................ttcagcccttgtgctccgtgcgggctaaccccttcggggcgttgaggggttgggttgttgaaatgtttagttgttga........................................................................................................................................................................................ ..........................................................................................................................................................................................................................ttcagcccttgggcctcgggcgagctaaccccttcggggcgttgaggtgttgaggtgttgagatgtttaattgttga........................................................................................................................................................................................ HSP 3 e-value: 2.0E-11 bit: 71.9 Len: 40 Query Start:375 Query End:414 Subject Strand: NEGATIVE Subject Start: 76 Subject End: 115 ......................................................................................................................................................................................................................................................................................................................................................................................ctgtaaaagccctgaaagggcgcattaattcagcccaatg................................................................. ......................................................................................................................................................................................................................................................................................................................................................................................ctgtaaaagccctgaaagggcgcgttaattcagcccaatg................................................................. Subject: tfor_TF0018_TF0019|hypothetical protein:heat shock protein ClpB|POSITIVE:POSITIVE|[12152,12589]|438 HSP 1 e-value: 5.0E-18 bit: 93.7 Len: 107 Query Start:308 Query End:414 Subject Strand: NEGATIVE Subject Start: 101 Subject End: 207 ...................................................................................................................................................................................................................................................................................................................ttgaggtgtttagccgttgaggtatttagttgttgaaatgtttagttgttgatctgtttactgaccactgtaaaagccctgaaagggcgcattaattcagcccaatg................................................................. ...................................................................................................................................................................................................................................................................................................................ttgaggtgttgagacgttgaggtgttgaggtgttgaggtgttgaattgttgaggtgtttgctgaccgttgtaacagccctgaaagggcgcattaattcagcccaatg................................................................. Subject: tfor_TF0179_TF0180|pyrophosphate-energized proton pump:carboxynorspermidine decarboxylase|POSITIVE:POSITIVE|[190752,191406]|655 HSP 1 e-value: 8.0E-17 bit: 89.7 Len: 57 Query Start:402 Query End:458 Subject Strand: NEGATIVE Subject Start: 217 Subject End: 273 .................................................................................................................................................................................................................................................................................................................................................................................................................attcagcccaatgcgaagcgttggggaaaaaggcatcgcatctcggcgaagggctgt..................... .................................................................................................................................................................................................................................................................................................................................................................................................................attcagcccaatgcgaagcgttgggaaaaaaggcatcgcgtctcggcggagggctgt..................... HSP 2 e-value: 1.0E-6 bit: 56.0 Len: 36 Query Start:193 Query End:228 Subject Strand: NEGATIVE Subject Start: 482 Subject End: 517 ................................................................................................................................................................................................aaataagaatgaatttaattcaggccttcagccctt........................................................................................................................................................................................................................................................... ................................................................................................................................................................................................aaataaggatgaatttaattcagaccttcagccctt........................................................................................................................................................................................................................................................... Subject: tfor_TF0585_TF0586|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[616824,617452]|629 HSP 1 e-value: 1.0E-15 bit: 85.7 Len: 51 Query Start:177 Query End:227 Subject Strand: POSITIVE Subject Start: 184 Subject End: 234 ................................................................................................................................................................................gattccgcaatagaagaaataagaatgaatttaattcaggccttcagccct............................................................................................................................................................................................................................................................ ................................................................................................................................................................................gattccgcaatagaaccaataagaatgaatttaattcaggccttcagccct............................................................................................................................................................................................................................................................ HSP 2 e-value: 2.0E-14 bit: 81.8 Len: 57 Query Start:402 Query End:458 Subject Strand: POSITIVE Subject Start: 388 Subject End: 444 .................................................................................................................................................................................................................................................................................................................................................................................................................attcagcccaatgcgaagcgttggggaaaaaggcatcgcatctcggcgaagggctgt..................... .................................................................................................................................................................................................................................................................................................................................................................................................................attcagcccaatgcgaagcgttggggaaaaaggcttcacatttcggcggagggctgt..................... Subject: tfor_TF1473_TF1474|hypothetical protein:6- phosphogluconolactonase|POSITIVE:POSITIVE|[1567027, 1567481]|455 HSP 1 e-value: 8.0E-14 bit: 79.8 Len: 52 Query Start:177 Query End:228 Subject Strand: NEGATIVE Subject Start: 327 Subject End: 378 ................................................................................................................................................................................gattccgcaatagaagaaataagaatgaatttaattcaggccttcagccctt........................................................................................................................................................................................................................................................... ................................................................................................................................................................................gattcggcaatagaacaaataaggatgaatttaattcaggccttcagccctt........................................................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-11 bit: 71.9 Len: 57 Query Start:402 Query End:458 Subject Strand: NEGATIVE Subject Start: 114 Subject End: 172 .................................................................................................................................................................................................................................................................................................................................................................................................................attcagcccaatgcgaagcgttgggg--aaaaaggcatcgcatctcggcgaagggctgt..................... .................................................................................................................................................................................................................................................................................................................................................................................................................attcagcccaatgcgaagcgttgggggaaaaaaagcatcgtatctcggcggagggctgt..................... Subject: tfor_TF2998_TF2999|surface antigen BspA:conserved hypothetical protein|POSITIVE:POSITIVE|[3215212,3215705]|494 HSP 1 e-value: 1.0E-12 bit: 75.8 Len: 58 Query Start:212 Query End:269 Subject Strand: NEGATIVE Subject Start: 343 Subject End: 399 ...................................................................................................................................................................................................................tcaggccttcagcccttgtgctccgtgcgggctaaccccttcggggcgttgaggggtt.................................................................................................................................................................................................................. ...................................................................................................................................................................................................................tcaggctttcagcccttgtgc-ccatgcgagctaaccccttcggggcgttgaggggtt.................................................................................................................................................................................................................. HSP 2 e-value: 2.0E-8 bit: 61.9 Len: 47 Query Start:375 Query End:421 Subject Strand: NEGATIVE Subject Start: 264 Subject End: 310 ......................................................................................................................................................................................................................................................................................................................................................................................ctgtaaaagccctgaaagggcgcattaattcagcccaatgcgaagcg.......................................................... ......................................................................................................................................................................................................................................................................................................................................................................................ctgtaagagccctgaaagggcgtaataattcagcccaatgcgtagcg.......................................................... Subject: tfor_TF2815_TF2816|meso-diaminopimelate D-dehydrogenase:hypothetical protein|POSITIVE:POSITIVE|[3006481,3007064]|584 HSP 1 e-value: 5.0E-12 bit: 73.8 Len: 37 Query Start:378 Query End:414 Subject Strand: NEGATIVE Subject Start: 258 Subject End: 294 .........................................................................................................................................................................................................................................................................................................................................................................................taaaagccctgaaagggcgcattaattcagcccaatg................................................................. .........................................................................................................................................................................................................................................................................................................................................................................................taaaagccctgaaagggcgcattaattcagcccaatg................................................................. HSP 2 e-value: 3.0E-7 bit: 58.0 Len: 45 Query Start:219 Query End:263 Subject Strand: NEGATIVE Subject Start: 358 Subject End: 402 ..........................................................................................................................................................................................................................ttcagcccttgtgctccgtgcgggctaaccccttcggggcgttga........................................................................................................................................................................................................................ ..........................................................................................................................................................................................................................ttcagcccttgtgccctgtgcgggctaatctcttcggggcgttga........................................................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUAUCCUCCCCCCAACAAAGAGCGCACUUCUCCGGCUUUUCGCAUCCCGUAAAGAGAAAACGGCCUUCGCUUAUCGGAAGAUUCCCCAAAAUCGUCCGAGCAAAAAAUCAUUUUAACCGUUCAUUCUAAUCUUUUUACGAAUCCAACUGUCAUCAAAUUUUCUUUUUAUCCCGGAUUCCGCAAUAGAAGAAAUAAGAAUGAAUUUAAUUCAGGCCUUCAGCCCUUGUGCUCCGUGCGGGCUAACCCCUUCGGGGCGUUGAGGGGUUGGGUUGUUGAAAUGUUUAGUUGUUGAAAUGUUUAGUCGUUGAGGUGUUUAGCCGUUGAGGUAUUUAGUUGUUGAAAUGUUUAGUUGUUGAUCUGUUUACUGACCACUGUAAAAGCCCUGAAAGGGCGCAUUAAUUCAGCCCAAUGCGAAGCGUUGGGGAAAAAGGCAUCGCAUCUCGGCGAAGGGCUGUUAAUUCAAUUGUUAAUUGUCA (((((((..((....((((...(((((.......(((.(((((..(.....)..)))))....)))....))))).(((((.((((......)))).))))).........(((((((((.(((((((((..((((((...((((((............................))))))......))))))....)))))))))..(((((((.((((((((......((((((...(((....)))...)))))))))))))).))))))))))))))))....))))..)).))))))).((((((((((((...(((..((((.....((((.((((...((..(((........)))..)).)))).)))).....((((.....)))).......)))).((((((((...))))))))......)))...))))))))))))..(((.((((((......)))))).))). (-132.89) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGACAAUUAACAAUUGAAUUAACAGCCCUUCGCCGAGAUGCGAUGCCUUUUUCCCCAACGCUUCGCAUUGGGCUGAAUUAAUGCGCCCUUUCAGGGCUUUUACAGUGGUCAGUAAACAGAUCAACAACUAAACAUUUCAACAACUAAAUACCUCAACGGCUAAACACCUCAACGACUAAACAUUUCAACAACUAAACAUUUCAACAACCCAACCCCUCAACGCCCCGAAGGGGUUAGCCCGCACGGAGCACAAGGGCUGAAGGCCUGAAUUAAAUUCAUUCUUAUUUCUUCUAUUGCGGAAUCCGGGAUAAAAAGAAAAUUUGAUGACAGUUGGAUUCGUAAAAAGAUUAGAAUGAACGGUUAAAAUGAUUUUUUGCUCGGACGAUUUUGGGGAAUCUUCCGAUAAGCGAAGGCCGUUUUCUCUUUACGGGAUGCGAAAAGCCGGAGAAGUGCGCUCUUUGUUGGGGGGAGGAUAUUUA .............(((((....((((((........(((((((.((.............)).))))))))))))).........((((.....))))........(((((........)))))............)))))....((((((((((...(......).(((((((((.....((((((..(((((...((..((..((.(((.(((((((........).))))))..((((....)).))....))).))..))..))........(((((....((((((.((((.(((...))).))))..))))))....))))).)))))..((((((........(((..(((((((..........(((((((((((.(((((....))))).)))))...)))))))))))))..)))........))))))........))))))......))))))))).)))).)))))) (-123.31) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1015 440 392 223 175 tfor:TF2166|5end_hypothetical_protein_2342115..2342345_POSITIVE 966 449 401 143 95 tfor:TF1274|5end_hypothetical_protein_1357621..1357851_POSITIVE 939 440 391 223 174 tfor:TF0340|5end_hypothetical_protein_363893..364123_POSITIVE 937 430 381 188 139 tfor:TF2641|5end_riboflavin_kinase/FAD_synthase_2809681..2809911_POSITIVE 932 458 414 81 37 tfor:TF2640|5end_hypothetical_protein_2809106..2809336_POSITIVE 910 430 382 213 165 tfor:TF0346|5end_hypothetical_protein_367048..367278_POSITIVE 900 257 212 231 187 tfor:TF2999|5end_conserved_hypothetical_protein_3215566..3215796_POSITIVE 836 420 375 226 176 tfor:TF2439|5end_conserved_hypothetical_protein_2616380..2616610_POSITIVE 814 439 392 231 184 tfor:TF1516|5end_transposase_fragment_1626407..1626637_POSITIVE 804 426 381 231 186 tfor:TF2351|5end_acetate_kinase_2515480..2515710_POSITIVE 804 449 402 170 122 tfor:TF0499|5end_hypothetical_protein_526257..526487_POSITIVE 777 424 381 231 188 tfor:TF1175|5end_membrane-fusion_protein_1258140..1258370_POSITIVE 730 220 173 154 107 tfor:TF3082|5end_conserved_hypothetical_protein_3316431..3316661_POSITIVE 724 227 181 179 133 tfor:TF2048|5end_conserved_hypothetical_protein_2210643..2210873_POSITIVE 724 227 181 154 108 tfor:TF1275|5end_transcription-repair_coupling_factor_1357866..1358096_POSITIVE 705 427 382 226 171 tfor:TF2172|5end_hypothetical_protein_2345822..2346052_POSITIVE 705 427 382 220 165 tfor:TF2173|5end_hypothetical_protein_2345816..2346046_POSITIVE 685 430 382 223 165 tfor:TF2171|5end_hypothetical_protein_2345485..2345715_POSITIVE 670 430 382 159 101 tfor:TF2127|5end_hypothetical_protein_2304038..2304268_POSITIVE 666 427 382 230 183 tfor:TF0468|5end_hypothetical_protein_486929..487159_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1017 449 402 137 90 tfor:TF2025|3end_hypothetical_protein_2182180..2182330_POSITIVE 891 430 383 48 1 tfor:TF0344|3end_hypothetical_protein_366963..367113_POSITIVE 862 430 382 125 77 tfor:TF1592|3end_hypothetical_protein_1708557..1708707_POSITIVE 834 429 383 47 1 tfor:TF1585|3end_hypothetical_protein_1690610..1690760_POSITIVE 815 430 382 125 77 tfor:TF1590|3end_hypothetical_protein_1706043..1706193_POSITIVE 784 430 381 60 1 tfor:TF0958|3end_conserved_hypothetical_protein_1004909..1005059_POSITIVE 737 436 391 47 2 tfor:TF1584|3end_hypothetical_protein_1690603..1690753_POSITIVE 724 227 181 103 57 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 706 420 375 146 101 tfor:TF1588|3end_hypothetical_protein_1702560..1702710_POSITIVE 705 427 382 116 61 tfor:TF2171|3end_hypothetical_protein_2345792..2345942_POSITIVE 699 457 414 62 19 tfor:TF2922|3end_surface_antigen_BspA_3121538..3121688_POSITIVE 689 436 392 44 1 tfor:TF2350|3end_hypothetical_protein_2515364..2515514_POSITIVE 679 257 212 138 80 tfor:TF2816|3end_hypothetical_protein_3007184..3007334_POSITIVE 648 457 414 71 25 tfor:TF1762|3end_hypothetical_protein_1899648..1899798_POSITIVE 601 457 415 44 1 tfor:TF0018|3end_hypothetical_protein_12091..12241_POSITIVE 591 430 382 74 24 tfor:TF2342|3end_glutathione_peroxidase_2506639..2506789_POSITIVE 462 290 248 89 49 tfor:TF1170|3end_conserved_hypothetical_protein_1252799..1252949_POSITIVE 438 269 221 150 89 tfor:TF1186|3end_hypothetical_protein_1269815..1269965_POSITIVE 437 258 212 83 24 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 434 457 414 129 93 tfor:TF1730|3end_diaminopimelate_decarboxylase_1855907..1856057_POSITIVE