Origin IGS: taacgaacataagcaattgttcgataatcaattatatccatgccataaagcagcaaggcagttaaagcataaaaaaagaatacaaagcacgatacttatacgaccctgctacattacaacatattacatatcaaaccatccaatctcatgataaaaagacccgatctccacttcattcacggataa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttatccgtgaatgaagtggagatcgggtctttttatcatgagattggatggtttgatatgtaatatgttgtaatgtagcagggtcgtataagtatcgtgctttgtattctttttttatgctttaactgccttgctgctttatggcatggatataattgattatcgaacaattgcttatgttcgtta Mask Tandem Repeat Region ================================================ taacgaacataagcaattgttcgataatcaattatatccatgccataaagcagcaaggcagttaaagcataaaaaaagaatacaaagcacgatacttatacgaccctgctacattacaacatattacatatcaaaccatccaatctcatgataaaaagacccgatctccacttcattcacggataa Find is-nt database================================================ Query_seq: TF1153:TF1154|TF1153:TF1154:pyruvate-formate lyase-activating enzyme:RNA pseudouridylate synthetase:->->:1235734..1235919 186 taacgaacataagcaattgttcgataatcaattatatccatgccataaagcagcaaggcagttaaagcataaaaaaagaatacaaagcacgatacttatacgaccctgctacattacaacatattacatatcaaaccatccaatctcatgataaaaagacccgatctccacttcattcacggataa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1153:TF1154|TF1153:TF1154:pyruvate-formate lyase-activating enzyme:RNA pseudouridylate synthetase:->->:1235734..1235919 186 taacgaacataagcaattgttcgataatcaattatatccatgccataaagcagcaaggcagttaaagcataaaaaaagaatacaaagcacgatacttatacgaccctgctacattacaacatattacatatcaaaccatccaatctcatgataaaaagacccgatctccacttcattcacggataa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1153:TF1154|TF1153:TF1154:pyruvate-formate lyase-activating enzyme:RNA pseudouridylate synthetase:->->:1235734..1235919 186 taacgaacataagcaattgttcgataatcaattatatccatgccataaagcagcaaggcagttaaagcataaaaaaagaatacaaagcacgatacttatacgaccctgctacattacaacatattacatatcaaaccatccaatctcatgataaaaagacccgatctccacttcattcacggataa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacgaacataagcaattgttcgataatcaattatatccatgccataaagcagcaaggcagttaaagcataaaaaaagaatacaaagcacgatacttatacgaccctgctacattacaacatattacatatcaaaccatccaatctcatgataaaaagacccgatctccacttcattcacggataa Predict ORF larger than 30AA ================================================ Protein_Len: 44 Strand: + Start: 17 End: 148 ................ M F D N Q L Y P C H K A A R Q L K H K K R I Q S T I L I R P C Y I T T Y Y I S N H P I S ...................................... Protein_Len: 39 Strand: + Start: 69 End: 185 .................................................................... M K K E Y K A R Y L Y D P A T L Q H I T Y Q T I Q S H D K K T R S P L H S R I . taacgaacataagcaattgttcgataatcaattatatccatgccataaagcagcaaggcagttaaagcataaaaaaagaatacaaagcacgatacttatacgaccctgctacattacaacatattacatatcaaaccatccaatctcatgataaaaagacccgatctccacttcattcacggataa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacgaacataagcaattgttcgataatcaattatatccatgccataaagcagcaaggcagttaaagcataaaaaaagaatacaaagcacgatacttatacgaccctgctacattacaacatattacatatcaaaccatccaatctcatgataaaaagacccgatctccacttcattcacggataa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1153:TF1154|TF1153:TF1154:pyruvate-formate lyase-activating enzyme:RNA pseudouridylate synthetase:->->:1235734..1235919 186 taacgaacataagcaattgttcgataatcaattatatccatgccataaagcagcaaggcagttaaagcataaaaaaagaatacaaagcacgatacttatacgaccctgctacattacaacatattacatatcaaaccatccaatctcatgataaaaagacccgatctccacttcattcacggataa Intra-Species Hit: Count: 1 Min: 1 Max: 186 Len: 186 Subject: tfor_TF1153_TF1154|pyruvate-formate lyase-activating enzyme:RNA pseudouridylate synthetase|POSITIVE:POSITIVE|[1235734,1235919]|186 HSP 1 e-value: 8.0E-89 bit: 327.0 Len: 186 Query Start:1 Query End:186 Subject Strand: POSITIVE Subject Start: 1 Subject End: 186 taacgaacataagcaattgttcgataatcaattatatccatgccataaagcagcaaggcagttaaagcatnnnnnnngaatacaaagcacgatacttatacgaccctgctacattacaacatattacatatcaaaccatccaatctcatgataaaaagacccgatctccacttcattcacggataa taacgaacataagcaattgttcgataatcaattatatccatgccataaagcagcaaggcagttaaagcataaaaaaagaatacaaagcacgatacttatacgaccctgctacattacaacatattacatatcaaaccatccaatctcatgataaaaagacccgatctccacttcattcacggataa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACGAACAUAAGCAAUUGUUCGAUAAUCAAUUAUAUCCAUGCCAUAAAGCAGCAAGGCAGUUAAAGCAUAAAAAAAGAAUACAAAGCACGAUACUUAUACGACCCUGCUACAUUACAACAUAUUACAUAUCAAACCAUCCAAUCUCAUGAUAAAAAGACCCGAUCUCCACUUCAUUCACGGAUAA ...((((((........))))))...........(((((.((((............)))).................((((....((((.(.............).))))..................(((((...............))))).....................))))..))))). (-21.88) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAUCCGUGAAUGAAGUGGAGAUCGGGUCUUUUUAUCAUGAGAUUGGAUGGUUUGAUAUGUAAUAUGUUGUAAUGUAGCAGGGUCGUAUAAGUAUCGUGCUUUGUAUUCUUUUUUUAUGCUUUAACUGCCUUGCUGCUUUAUGGCAUGGAUAUAAUUGAUUAUCGAACAAUUGCUUAUGUUCGUUA ........((((((((..(..(((.((((((........)))))).))).((((((((((((.(((((..(((.((((((((((.((..((((((.....................))))))..)).)))))))))).)))..)))))..))))......))))))))..)..))).))))).... (-51.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 484 90 42 129 80 tfor:TF0286|5end_hypothetical_protein_315543..315773_POSITIVE 379 127 84 223 179 tfor:TF1328|5end_possible_cytidylyltransferase_1407434..1407664_POSITIVE 329 59 18 176 135 tfor:TF0109|5end_diacylglycerol_kinase_116073..116303_POSITIVE 313 89 48 97 57 tfor:TF1764|5end_transcriptional_regulator;_possible_MerR_family_1901409..1901639_POSITIVE 301 60 11 62 2 tfor:TF1714|5end_hypothetical_protein_1834125..1834355_POSITIVE 284 159 112 115 62 tfor:TF2097|5end_glucose_inhibited_division_protein_A_2269137..2269367_POSITIVE 281 178 137 200 164 tfor:TF3096|5end_conserved_hypothetical_protein_3327963..3328193_POSITIVE 267 74 32 81 39 tfor:TF3098|5end_conserved_hypothetical_protein_3329913..3330143_POSITIVE 257 69 43 65 36 tfor:TF0610|5end_conserved_hypothetical_protein;_possible_phosphatidylglycerophosphate_synthase_643460..643690_POSITIVE 252 139 95 187 139 tfor:TF2883|5end_peptidyl-prolyl_cis-trans_isomerase_3074321..3074551_POSITIVE 248 180 135 217 165 tfor:TF1755|5end_periplasmic_protease_1891774..1892004_POSITIVE 246 60 13 55 8 tfor:TF1958|5end_cytidylate_kinase_2108659..2108889_POSITIVE 244 70 21 217 172 tfor:TF2523|5end_NAD(P)H-flavin_oxidoreductase_2702114..2702344_POSITIVE 244 54 11 95 57 tfor:TF1748|5end_conserved_hypothetical_protein_1882558..1882788_POSITIVE 243 60 11 143 83 tfor:TF0738|5end_conserved_hypothetical_protein_788643..788873_POSITIVE 243 68 22 193 144 tfor:TF0175|5end_hypothetical_protein_185585..185815_POSITIVE 240 89 45 61 11 tfor:TF1983|5end_conserved_hypothetical_protein_2134684..2134914_POSITIVE 240 89 45 61 11 tfor:TF1905|5end_conserved_hypothetical_protein_2059932..2060162_POSITIVE 239 71 40 48 12 tfor:TF1418|5end_cell_division_protein_1500027..1500257_POSITIVE 238 82 47 156 111 tfor:TF2923|5end_hypothetical_protein_3122168..3122398_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 330 99 55 44 1 tfor:TF0285|3end_conserved_hypothetical_protein_315534..315684_POSITIVE 313 89 48 89 49 tfor:TF1763|3end_K+-dependent_Na+/Ca+_exchanger_related-protein_1901481..1901631_POSITIVE 273 68 43 23 1 tfor:TF0108|3end_thiamine_biosynthesis_protein,_ThiF_family_116000..116150_POSITIVE 272 90 41 50 6 tfor:TF2830|3end_ABC_transporter,_ATP-binding_protein_3021419..3021569_POSITIVE 267 74 32 49 7 tfor:TF3097|3end_conserved_hypothetical_protein_3329961..3330111_POSITIVE 261 90 41 59 12 tfor:TF1087|3end_ISPg4_transposase_1158225..1158375_POSITIVE 246 60 13 48 1 tfor:TF1957|3end_penicillin_tolerance_protein;_possible_isoprene_biosynthesis_related_protein_2108732..2108882_POSITIVE 243 68 22 128 79 tfor:TF0174|3end_hypothetical_protein_185600..185750_POSITIVE 237 110 66 64 8 tfor:TF0380|3end_conserved_hypothetical_protein_401633..401783_POSITIVE 233 79 41 79 38 tfor:TF0855|3end_membrane_protein,_possible_exporter_908199..908349_POSITIVE 232 127 89 122 75 tfor:TF2456|3end_ABC_transporter,_ATP-binding_protein_2633208..2633358_POSITIVE 232 69 35 66 28 tfor:TF0606|3end_signal_peptidase_II_637504..637654_POSITIVE 231 69 21 151 107 tfor:TF2522|3end_deoxyuridine_5'-triphosphate_nucleotidohydrolase_(dUTPase)_2702129..2702279_POSITIVE 229 117 84 62 13 tfor:TF0759|3end_heat_shock_protein_15,_RNA-binding_domain_S4_806366..806516_POSITIVE 221 68 32 140 114 tfor:TF0758|3end_peptidyl-tRNA_hydrolase_805950..806100_POSITIVE 220 59 35 60 34 tfor:TF1110|3end_conserved_hypothetical_protein_1180837..1180987_POSITIVE 218 60 13 50 14 tfor:TF1883|3end_conserved_hypothetical_protein_2035865..2036015_POSITIVE 216 180 153 91 59 tfor:TF1244|3end_hypothetical_protein_1328813..1328963_POSITIVE 214 60 11 122 64 tfor:TF2181|3end_conserved_hypothetical_protein_2354963..2355113_POSITIVE 214 67 38 47 10 tfor:TF1627|3end_guanylate_kinase_1740704..1740854_POSITIVE