Origin IGS: tgataagttgttgattgaattaatttattgttatgttcactttgcatatgatgtatggaaagtatttgcggattaaaaaaatggaagaatgaaataaaatatattgctccatgatacgaacgatttatctacttatacatctttattgactctaatcggcagtggataaatgctgttctgctgttatctacccgatgtcatttacataagaaacccaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttgggtttcttatgtaaatgacatcgggtagataacagcagaacagcatttatccactgccgattagagtcaataaagatgtataagtagataaatcgttcgtatcatggagcaatatattttatttcattcttccatttttttaatccgcaaatactttccatacatcatatgcaaagtgaacataacaataaattaattcaatcaacaacttatca Mask Tandem Repeat Region ================================================ tgataagttgttgattgaattaatttattgttatgttcactttgcatatgatgtatggaaagtatttgcggattaaaaaaatggaagaatgaaataaaatatattgctccatgatacgaacgatttatctacttatacatctttattgactctaatcggcagtggataaatgctgttctgctgttatctacccgatgtcatttacataagaaacccaaa Find is-nt database================================================ Query_seq: TF1127:TF1128|TF1127:TF1128:conserved hypothetical protein; possible NADPH:quinone reductase:possible lantibiotic biosynthesis protein (modification):->->:1202218..1202436 219 tgataagttgttgattgaattaatttattgttatgttcactttgcatatgatgtatggaaagtatttgcggattaaaaaaatggaagaatgaaataaaatatattgctccatgatacgaacgatttatctacttatacatctttattgactctaatcggcagtggataaatgctgttctgctgttatctacccgatgtcatttacataagaaacccaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1127:TF1128|TF1127:TF1128:conserved hypothetical protein; possible NADPH:quinone reductase:possible lantibiotic biosynthesis protein (modification):->->:1202218..1202436 219 tgataagttgttgattgaattaatttattgttatgttcactttgcatatgatgtatggaaagtatttgcggattaaaaaaatggaagaatgaaataaaatatattgctccatgatacgaacgatttatctacttatacatctttattgactctaatcggcagtggataaatgctgttctgctgttatctacccgatgtcatttacataagaaacccaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1127:TF1128|TF1127:TF1128:conserved hypothetical protein; possible NADPH:quinone reductase:possible lantibiotic biosynthesis protein (modification):->->:1202218..1202436 219 tgataagttgttgattgaattaatttattgttatgttcactttgcatatgatgtatggaaagtatttgcggattaaaaaaatggaagaatgaaataaaatatattgctccatgatacgaacgatttatctacttatacatctttattgactctaatcggcagtggataaatgctgttctgctgttatctacccgatgtcatttacataagaaacccaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgataagttgttgattgaattaatttattgttatgttcactttgcatatgatgtatggaaagtatttgcggattaaaaaaatggaagaatgaaataaaatatattgctccatgatacgaacgatttatctacttatacatctttattgactctaatcggcagtggataaatgctgttctgctgttatctacccgatgtcatttacataagaaacccaaa Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 127 End: 219 .............................................................................................................................. M Y L Y I F I D S N R Q W I N A V L L L S T R C H L H K K P K Protein_Len: 34 Strand: - Start: 23 End: 124 ...................... N I T I N V K C I I Y P F Y K R I L F I S S H F L I Y Q E M I R V M ............................................................................................... Protein_Len: 42 Strand: - Start: 3 End: 128 .. Y T T S Q I L K N N H E S Q M H H I S L I Q P N F F H F F S I F Y I A G H Y S R N M ........................................................................................... tgataagttgttgattgaattaatttattgttatgttcactttgcatatgatgtatggaaagtatttgcggattaaaaaaatggaagaatgaaataaaatatattgctccatgatacgaacgatttatctacttatacatctttattgactctaatcggcagtggataaatgctgttctgctgttatctacccgatgtcatttacataagaaacccaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgataagttgttgattgaattaatttattgttatgttcactttgcatatgatgtatggaaagtatttgcggattaaaaaaatggaagaatgaaataaaatatattgctccatgatacgaacgatttatctacttatacatctttattgactctaatcggcagtggataaatgctgttctgctgttatctacccgatgtcatttacataagaaacccaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1127:TF1128|TF1127:TF1128:conserved hypothetical protein; possible NADPH:quinone reductase:possible lantibiotic biosynthesis protein (modification):->->:1202218..1202436 219 tgataagttgttgattgaattaatttattgttatgttcactttgcatatgatgtatggaaagtatttgcggattaaaaaaatggaagaatgaaataaaatatattgctccatgatacgaacgatttatctacttatacatctttattgactctaatcggcagtggataaatgctgttctgctgttatctacccgatgtcatttacataagaaacccaaa Intra-Species Hit: Count: 1 Min: 1 Max: 219 Len: 219 Subject: tfor_TF1127_TF1128|conserved hypothetical protein; possible NADPH:quinone reductase:possible lantibiotic biosynthesis protein (modification)|POSITIVE:POSITIVE|[1202218,1202436]|219 HSP 1 e-value: 1.0E-108 bit: 392.0 Len: 219 Query Start:1 Query End:219 Subject Strand: POSITIVE Subject Start: 1 Subject End: 219 tgataagttgttgattgaattaatttattgttatgttcactttgcatatgatgtatggaaagtatttgcggattnnnnnnntggaagaatgaaataaaatatattgctccatgatacgaacgatttatctacttatacatctttattgactctaatcggcagtggataaatgctgttctgctgttatctacccgatgtcatttacataagaaacccaaa tgataagttgttgattgaattaatttattgttatgttcactttgcatatgatgtatggaaagtatttgcggattaaaaaaatggaagaatgaaataaaatatattgctccatgatacgaacgatttatctacttatacatctttattgactctaatcggcagtggataaatgctgttctgctgttatctacccgatgtcatttacataagaaacccaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUAAGUUGUUGAUUGAAUUAAUUUAUUGUUAUGUUCACUUUGCAUAUGAUGUAUGGAAAGUAUUUGCGGAUUAAAAAAAUGGAAGAAUGAAAUAAAAUAUAUUGCUCCAUGAUACGAACGAUUUAUCUACUUAUACAUCUUUAUUGACUCUAAUCGGCAGUGGAUAAAUGCUGUUCUGCUGUUAUCUACCCGAUGUCAUUUACAUAAGAAACCCAAA .(((((((((((...............(((...(((..(((((.(((((...))))).)))))....)))...)))....(((((..((((..........))))..)))))......)))))))))))..........((((...((((....(((((..((((((((..((......))..))))))))))))))))).......))))........ (-42.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUGGGUUUCUUAUGUAAAUGACAUCGGGUAGAUAACAGCAGAACAGCAUUUAUCCACUGCCGAUUAGAGUCAAUAAAGAUGUAUAAGUAGAUAAAUCGUUCGUAUCAUGGAGCAAUAUAUUUUAUUUCAUUCUUCCAUUUUUUUAAUCCGCAAAUACUUUCCAUACAUCAUAUGCAAAGUGAACAUAACAAUAAAUUAAUUCAAUCAACAACUUAUCA .(((((((..((((....(((.....((((((((....((......))))))))))..(((.((((((((......((((.........(((((.((.((((........)))).)).)))))........))))......)))))))).)))..((((((.((((.....)))).))))))..)))....))))...))))))).............. (-34.83) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 359 196 153 71 28 tfor:TF0569|5end_indolepyruvate_ferredoxin_oxidoreductase,_beta_subunit_601723..601953_POSITIVE 332 200 157 47 1 tfor:TF2437|5end_elongation_factor_P_2615104..2615334_POSITIVE 331 199 155 223 191 tfor:TF2373|5end_UDP-3-O-acyl-GlcNAc_deacetylase_2538344..2538574_POSITIVE 329 195 156 197 158 tfor:TF2430|5end_hypothetical_protein_2612660..2612890_POSITIVE 326 200 151 195 147 tfor:TF2261|5end_transfer_region-related_protein,_TraM_2430496..2430726_POSITIVE 306 183 141 88 54 tfor:TF2847|5end_hypothetical_protein_3034744..3034974_POSITIVE 299 199 156 79 24 tfor:TF2747|5end_heat_shock_protein,_HSP90_family_2928279..2928509_POSITIVE 298 200 151 129 75 tfor:TF1891|5end_hypothetical_protein_2043389..2043619_POSITIVE 298 197 151 207 159 tfor:TF0006|5end_3-oxyacyl_[acyl-carrier_protein]_reductase_1757..1987_POSITIVE 298 197 151 186 138 tfor:TF0004|5end_conserved_hypothetical_protein_1736..1966_POSITIVE 290 199 153 183 137 tfor:TF2189|5end_conserved_hypothetical_protein_2363455..2363685_POSITIVE 289 197 155 122 72 tfor:TF1856|5end_conserved_hypothetical_protein_2009969..2010199_POSITIVE 286 194 151 151 103 tfor:TF1001|5end_penicillin-binding_protein_1A_1054097..1054327_POSITIVE 285 200 153 191 131 tfor:TF0200|5end_2-nitropropane_dioxygenase_225061..225291_POSITIVE 284 192 158 77 42 tfor:TF2895|5end_hypothetical_protein_3083936..3084166_POSITIVE 282 194 157 33 1 tfor:TF2771|5end_undecaprenol_kinase,_bacitracin_resistance_protein_2956559..2956789_POSITIVE 282 196 155 172 137 tfor:TF2186|5end_outer_membrane_protein_2360291..2360521_POSITIVE 281 190 143 203 164 tfor:TF0376|5end_conserved_hypothetical_protein_394303..394533_POSITIVE 280 200 157 207 159 tfor:TF2278|5end_conserved_hypothetical_protein_2438662..2438892_POSITIVE 279 197 155 221 185 tfor:TF1502|5end_exo-poly-alpha-D-galacturonosidase_precursor_1602796..1603026_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 359 196 153 66 23 tfor:TF0568|3end_indolepyruvate_ferredoxin_oxidoreductase,_alpha_subunit_601798..601948_POSITIVE 304 187 150 102 63 tfor:TF2480|3end_hypothetical_protein_2657708..2657858_POSITIVE 292 181 144 151 108 tfor:TF2020|3end_muconate_cycloisomerase_2177552..2177702_POSITIVE 286 194 151 143 95 tfor:TF1000|3end_sec-independent_protein_translocase_1054169..1054319_POSITIVE 282 194 157 33 1 tfor:TF2770|3end_conserved_hypothetical_protein_2956639..2956789_POSITIVE 282 200 156 33 4 tfor:TF2230|3end_CTn_excision_protein_2400394..2400544_POSITIVE 278 199 158 53 13 tfor:TF3153|3end_conserved_hypothetical_protein_3389324..3389474_POSITIVE 269 194 154 150 99 tfor:TF3143|3end_conserved_hypothetical_protein;_possible_membrane_protein_3379227..3379377_POSITIVE 266 188 143 120 75 tfor:TF2453|3end_glutamate_racemase_2631326..2631476_POSITIVE 262 197 158 109 61 tfor:TF1247|3end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_1330928..1331078_POSITIVE 260 195 156 99 49 tfor:TF3003|3end_hypothetical_protein_3220629..3220779_POSITIVE 260 200 151 84 31 tfor:TF0464|3end_precorrin_methylase_482108..482258_POSITIVE 256 200 151 145 87 tfor:TF2795|3end_hypothetical_protein_2980909..2981059_POSITIVE 256 201 161 146 114 tfor:TF1443|3end_hypothetical_protein_1528203..1528353_POSITIVE 255 194 152 86 47 tfor:TF1994|3end_acyl-CoA_synthetase_2147228..2147378_POSITIVE 252 200 156 79 26 tfor:TF1249|3end_phosphoglycerate_kinase_(cytosolic_phosphoglycerate_kinase)_1332356..1332506_POSITIVE 249 195 158 72 39 tfor:TF2487|3end_transposase_2663956..2664106_POSITIVE 249 160 111 122 75 tfor:TF2297|3end_hypothetical_protein_2449673..2449823_POSITIVE 249 198 158 62 15 tfor:TF2118|3end_conserved_hypothetical_protein_2291693..2291843_POSITIVE 249 199 155 137 94 tfor:TF1909|3end_cobalamin_(5`-phosphate)_synthase_2064075..2064225_POSITIVE