Origin IGS: taaaaacgatataatgatatattttgaaaggtatttctatggggcagtgaaagtgcgcaatgaataagtgtcttttcaatcggtcactcggtatttccggataaagatagcgtgtgtttgtgtggatttatgaaacggcagttaagggcaaatataccgtttatcggaaaaaaacgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaagaaagggaggataattatctttgctcccgacatattaaaattttaccgtgatgaaaaaattagaaaaactaagattgaaaaaaggaaaatcttttgaggttattgactatggattgtctaaaagtcaattgtcccatatggaacagtcttttcttattggaggggtatatgcttgtgcaggcaatgcctgtggaggtgatgcttgtgtaactgatgcatgtggaggtaatgcatgcggtggtgacggttgtctaataaacgcatgtggtggaaacgcatgtcctgtacaattgtgtcccattgacgcttgttctgttgatggttgctggattgatgcatttcattcataagtgtagtgtataatgt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 acattatacactacacttatgaatgaaatgcatcaatccagcaaccatcaacagaacaagcgtcaatgggacacaattgtacaggacatgcgtttccaccacatgcgtttattagacaaccgtcaccaccgcatgcattacctccacatgcatcagttacacaagcatcacctccacaggcattgcctgcacaagcatatacccctccaataagaaaagactgttccatatgggacaattgacttttagacaatccatagtcaataacctcaaaagattttccttttttcaatcttagtttttctaattttttcatcacggtaaaattttaatatgtcgggagcaaagataattatcctccctttcttgctccaatttatactccgcttttctaacgaacggtagcatttttcctaacgaacggtcgtttttttccgataaacggtatatttgcccttaactgccgtttcataaatccacacaaacacacgctatctttatccggaaataccgagtgaccgattgaaaagacacttattcattgcgcactttcactgccccatagaaatacctttcaaaatatatcattatatcgttttta Mask Tandem Repeat Region ================================================ taaaaacgatataatgatatattttgaaaggtatttctatggggcagtgaaagtgcgcaatgaataagtgtcttttcaatcggtcactcggtatttccggataaagatagcgtgtgtttgtgtggatttatgaaacggcagttaagggcaaatataccgtttatcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngcggagtataaattggagcaagaaagggaggataattatctttgctcccgacatattaaaattttaccgtgatgaaaaaattagaaaaactaagattgaaaaaaggaaaatcttttgaggttattgactatggattgtctaaaagtcaattgtcccatatggaacagtcttttcttattggaggggtatatgcttgtgcaggcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncggtggtgacggttgtctaataaacgcatgtggtggaaacgcatgtcctgtacaattgtgtcccattgacgcttgttctgttgatggttgctggattgatgcatttcattcataagtgtagtgtataatgt Find is-nt database================================================ Query_seq: TF1104:TF1105|TF1104:TF1105:hypothetical protein:arylsulfatase regulator; Fe-S oxidoreductase:->->:1172255..1172855 601 taaaaacgatataatgatatattttgaaaggtatttctatggggcagtgaaagtgcgcaatgaataagtgtcttttcaatcggtcactcggtatttccggataaagatagcgtgtgtttgtgtggatttatgaaacggcagttaagggcaaatataccgtttatcggaaaaaaacgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaagaaagggaggataattatctttgctcccgacatattaaaattttaccgtgatgaaaaaattagaaaaactaagattgaaaaaaggaaaatcttttgaggttattgactatggattgtctaaaagtcaattgtcccatatggaacagtcttttcttattggaggggtatatgcttgtgcaggcaatgcctgtggaggtgatgcttgtgtaactgatgcatgtggaggtaatgcatgcggtggtgacggttgtctaataaacgcatgtggtggaaacgcatgtcctgtacaattgtgtcccattgacgcttgttctgttgatggttgctggattgatgcatttcattcataagtgtagtgtataatgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1104:TF1105|TF1104:TF1105:hypothetical protein:arylsulfatase regulator; Fe-S oxidoreductase:->->:1172255..1172855 601 taaaaacgatataatgatatattttgaaaggtatttctatggggcagtgaaagtgcgcaatgaataagtgtcttttcaatcggtcactcggtatttccggataaagatagcgtgtgtttgtgtggatttatgaaacggcagttaagggcaaatataccgtttatcggaaaaaaacgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaagaaagggaggataattatctttgctcccgacatattaaaattttaccgtgatgaaaaaattagaaaaactaagattgaaaaaaggaaaatcttttgaggttattgactatggattgtctaaaagtcaattgtcccatatggaacagtcttttcttattggaggggtatatgcttgtgcaggcaatgcctgtggaggtgatgcttgtgtaactgatgcatgtggaggtaatgcatgcggtggtgacggttgtctaataaacgcatgtggtggaaacgcatgtcctgtacaattgtgtcccattgacgcttgttctgttgatggttgctggattgatgcatttcattcataagtgtagtgtataatgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1104:TF1105|TF1104:TF1105:hypothetical protein:arylsulfatase regulator; Fe-S oxidoreductase:->->:1172255..1172855 601 taaaaacgatataatgatatattttgaaaggtatttctatggggcagtgaaagtgcgcaatgaataagtgtcttttcaatcggtcactcggtatttccggataaagatagcgtgtgtttgtgtggatttatgaaacggcagttaagggcaaatataccgtttatcggaaaaaaacgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaagaaagggaggataattatctttgctcccgacatattaaaattttaccgtgatgaaaaaattagaaaaactaagattgaaaaaaggaaaatcttttgaggttattgactatggattgtctaaaagtcaattgtcccatatggaacagtcttttcttattggaggggtatatgcttgtgcaggcaatgcctgtggaggtgatgcttgtgtaactgatgcatgtggaggtaatgcatgcggtggtgacggttgtctaataaacgcatgtggtggaaacgcatgtcctgtacaattgtgtcccattgacgcttgttctgttgatggttgctggattgatgcatttcattcataagtgtagtgtataatgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaaacgatataatgatatattttgaaaggtatttctatggggcagtgaaagtgcgcaatgaataagtgtcttttcaatcggtcactcggtatttccggataaagatagcgtgtgtttgtgtggatttatgaaacggcagttaagggcaaatataccgtttatcggaaaaaaacgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaagaaagggaggataattatctttgctcccgacatattaaaattttaccgtgatgaaaaaattagaaaaactaagattgaaaaaaggaaaatcttttgaggttattgactatggattgtctaaaagtcaattgtcccatatggaacagtcttttcttattggaggggtatatgcttgtgcaggcaatgcctgtggaggtgatgcttgtgtaactgatgcatgtggaggtaatgcatgcggtggtgacggttgtctaataaacgcatgtggtggaaacgcatgtcctgtacaattgtgtcccattgacgcttgttctgttgatggttgctggattgatgcatttcattcataagtgtagtgtataatgt Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: + Start: 64 End: 207 ............................................................... M S V F S I G H S V F P D K D S V C L C G F M K R Q L R A N I P F I G K K R P F V R K N A T V R .......................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 44 Strand: + Start: 152 End: 283 ....................................................................................................................................................... M Y R L S E K N D R S L G K M L P F V R K A E Y K L E Q E R E D N Y L C S R H I K I L P .............................................................................................................................................................................................................................................................................................................................. Protein_Len: 36 Strand: + Start: 222 End: 329 ............................................................................................................................................................................................................................. M N W S K K G R I I I F A P D I L K F Y R D E K I R K T K I E K R K I F ................................................................................................................................................................................................................................................................................ Protein_Len: 38 Strand: + Start: 329 End: 442 ........................................................................................................................................................................................................................................................................................................................................ M R L L T M D C L K V N C P I W N S L F L L E G Y M L V Q A M P V E V M L V ............................................................................................................................................................... Protein_Len: 60 Strand: + Start: 283 End: 582 .......................................................................................................................................................................................................................................................................................... M M K K L E K L R L K K G K S F E V I D Y G L S K S Q L S H M E Q S F L I G G V Y A C A G N A C G G D A C V T D A C G G ................... Protein_Len: 32 Strand: + Start: 500 End: 595 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M V E T H V L Y N C V P L T L V L L M V A G L M H F I H K C S V ...... Protein_Len: 30 Strand: - Start: 490 End: 579 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Y V C T T S V C T R Y L Q T G N V S T R N I T A P N I C K M ...................... Protein_Len: 60 Strand: - Start: 392 End: 583 ....................................................................................................................................................................................................................................................................................................................................................................................................... H K H L C H R H L H H K H L Q H M H L Y H M R H H R N D L L R M H H F R M D Q V I T D W Q R K N Q Q H N S S Q H M E N M .................. Protein_Len: 60 Strand: - Start: 363 End: 566 .......................................................................................................................................................................................................................................................................................................................................................................... R I P P T Y A Q A P L A Q P P S A Q T V S A H P P L A H P P S P Q R I F A H P P F A H G T C N H G M S A Q E T S P Q Q M ................................... Protein_Len: 53 Strand: - Start: 307 End: 465 .................................................................................................................................................................................................................................................................................................................. S Q F F S F R K L N N V I S Q R F T L Q G M H F L R K K N S P Y I S T C A I G T S T I S T Y S I C T S T M ........................................................................................................................................ Protein_Len: 42 Strand: - Start: 2 End: 127 . F V I Y H Y I K F P I E I P C H F H A I F L H R K L R D S P I E P Y L Y R T N T H M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... taaaaacgatataatgatatattttgaaaggtatttctatggggcagtgaaagtgcgcaatgaataagtgtcttttcaatcggtcactcggtatttccggataaagatagcgtgtgtttgtgtggatttatgaaacggcagttaagggcaaatataccgtttatcggaaaaaaacgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaagaaagggaggataattatctttgctcccgacatattaaaattttaccgtgatgaaaaaattagaaaaactaagattgaaaaaaggaaaatcttttgaggttattgactatggattgtctaaaagtcaattgtcccatatggaacagtcttttcttattggaggggtatatgcttgtgcaggcaatgcctgtggaggtgatgcttgtgtaactgatgcatgtggaggtaatgcatgcggtggtgacggttgtctaataaacgcatgtggtggaaacgcatgtcctgtacaattgtgtcccattgacgcttgttctgttgatggttgctggattgatgcatttcattcataagtgtagtgtataatgt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaaacgatataatgatatattttgaaaggtatttctatggggcagtgaaagtgcgcaatgaataagtgtcttttcaatcggtcactcggtatttccggataaagatagcgtgtgtttgtgtggatttatgaaacggcagttaagggcaaatataccgtttatcggaaaaaaacgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaagaaagggaggataattatctttgctcccgacatattaaaattttaccgtgatgaaaaaattagaaaaactaagattgaaaaaaggaaaatcttttgaggttattgactatggattgtctaaaagtcaattgtcccatatggaacagtcttttcttattggaggggtatatgcttgtgcaggcaatgcctgtggaggtgatgcttgtgtaactgatgcatgtggaggtaatgcatgcggtggtgacggttgtctaataaacgcatgtggtggaaacgcatgtcctgtacaattgtgtcccattgacgcttgttctgttgatggttgctggattgatgcatttcattcataagtgtagtgtataatgt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1104:TF1105|TF1104:TF1105:hypothetical protein:arylsulfatase regulator; Fe-S oxidoreductase:->->:1172255..1172855 601 taaaaacgatataatgatatattttgaaaggtatttctatggggcagtgaaagtgcgcaatgaataagtgtcttttcaatcggtcactcggtatttccggataaagatagcgtgtgtttgtgtggatttatgaaacggcagttaagggcaaatataccgtttatcggaaaaaaacgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaagaaagggaggataattatctttgctcccgacatattaaaattttaccgtgatgaaaaaattagaaaaactaagattgaaaaaaggaaaatcttttgaggttattgactatggattgtctaaaagtcaattgtcccatatggaacagtcttttcttattggaggggtatatgcttgtgcaggcaatgcctgtggaggtgatgcttgtgtaactgatgcatgtggaggtaatgcatgcggtggtgacggttgtctaataaacgcatgtggtggaaacgcatgtcctgtacaattgtgtcccattgacgcttgttctgttgatggttgctggattgatgcatttcattcataagtgtagtgtataatgt Intra-Species Hit: Count: 6 Min: 1 Max: 601 Len: 601 Subject: tfor_TF1104_TF1105|hypothetical protein:arylsulfatase regulator; Fe-S oxidoreductase|POSITIVE:POSITIVE|[1172255,1172855]|601 HSP 1 e-value: 1.0E-155 bit: 549.0 Len: 277 Query Start:325 Query End:601 Subject Strand: POSITIVE Subject Start: 325 Subject End: 601 ....................................................................................................................................................................................................................................................................................................................................tcttttgaggttattgactatggattgtctaaaagtcaattgtcccatatggaacagtcttttcttattggaggggtatatgcttgtgcaggcaatgcctgtggaggtgatgcttgtgtaactgatgcatgtggaggtaatgcatgcggtggtgacggttgtctaataaacgcatgtggtggaaacgcatgtcctgtacaattgtgtcccattgacgcttgttctgttgatggttgctggattgatgcatttcattcataagtgtagtgtataatgt ....................................................................................................................................................................................................................................................................................................................................tcttttgaggttattgactatggattgtctaaaagtcaattgtcccatatggaacagtcttttcttattggaggggtatatgcttgtgcaggcaatgcctgtggaggtgatgcttgtgtaactgatgcatgtggaggtaatgcatgcggtggtgacggttgtctaataaacgcatgtggtggaaacgcatgtcctgtacaattgtgtcccattgacgcttgttctgttgatggttgctggattgatgcatttcattcataagtgtagtgtataatgt HSP 2 e-value: 1.0E-148 bit: 527.0 Len: 287 Query Start:1 Query End:287 Subject Strand: POSITIVE Subject Start: 1 Subject End: 287 taaaaacgatataatgatatattttgaaaggtatttctatggggcagtgaaagtgcgcaatgaataagtgtcttttcaatcggtcactcggtatttccggataaagatagcgtgtgtttgtgtggatttatgaaacggcagttaagggcaaatataccgtttatcggnnnnnnncgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaagaaagggaggataattatctttgctcccgacatattaaaattttaccgtgat.......................................................................................................................................................................................................................................................................................................................... taaaaacgatataatgatatattttgaaaggtatttctatggggcagtgaaagtgcgcaatgaataagtgtcttttcaatcggtcactcggtatttccggataaagatagcgtgtgtttgtgtggatttatgaaacggcagttaagggcaaatataccgtttatcggaaaaaaacgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaagaaagggaggataattatctttgctcccgacatattaaaattttaccgtgat.......................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF1117_TF1118|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1189512,1191445]|1934 HSP 1 e-value: 1.0E-117 bit: 422.0 Len: 254 Query Start:34 Query End:287 Subject Strand: POSITIVE Subject Start: 1382 Subject End: 1635 .................................tttctatggggcagtgaaagtgcgcaatgaataagtgtcttttcaatcggtcactcggtatttccggataaagatagcgtgtgtttgtgtggatttatgaaacggcagttaagggcaaatataccgtttatcggnnnnnnncgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaagaaagggaggataattatctttgctcccgacatattaaaattttaccgtgat.......................................................................................................................................................................................................................................................................................................................... .................................tttctatggggcggtgaaagtgcgcaatgaataagtgtcttttcaattagtcactcggtatttccggataaagatagcgtgtgtttgtgtggatttatgaaacagcagtcaagggcaaatataccgtttatcggaaaaaaacgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaagaaagggaggataattatctttgctcccgacatattaaaattttaccgtgat.......................................................................................................................................................................................................................................................................................................................... Subject: tfor_TF1096_TF1097|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1165418,1165707]|290 HSP 1 e-value: 1.0E-110 bit: 398.0 Len: 277 Query Start:4 Query End:280 Subject Strand: POSITIVE Subject Start: 9 Subject End: 286 ...aaacgatataatgatatattttgaaaggtattt-ctatggggcagtgaaagtgcgcaatgaataagtgtcttttcaatcggtcactcggtatttccggataaagatagcgtgtgtttgtgtggatttatgaaacggcagttaagggcaaatataccgtttatcggnnnnnnncgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaagaaagggaggataattatctttgctcccgacatattaaaatttta................................................................................................................................................................................................................................................................................................................................. ...aaacgatataatgatatattttgaaaggtattttctatggggcggtgaaagtgcgcaatgaataagtgttttttcaatcggtcactcggtatttccggataaagatagcgtgtgtttgtgtggatttatgaaacactggtcaagagcaaatataccgtttatcgggaaaaaacgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattagagcaagagggagaggataattatctttgctcccgacatattaaaatttta................................................................................................................................................................................................................................................................................................................................. Subject: tfor_TF1071_TF1072|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1141748,1142530]|783 HSP 1 e-value: 3.0E-51 bit: 204.0 Len: 152 Query Start:129 Query End:280 Subject Strand: POSITIVE Subject Start: 93 Subject End: 244 ................................................................................................................................tatgaaacggcagttaagggcaaatataccgtttatcggnnnnnnncgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaagaaagggaggataattatctttgctcccgacatattaaaatttta................................................................................................................................................................................................................................................................................................................................. ................................................................................................................................tatgaaacggcagtcaagggcaaatataccgtttatcgaaaaaaaacgaccgttcgttaggaaaaatgctactgttcgttagaaaagcggagtataaattggagcaagagggagaggatgattatctttgctcccgacatattaaaatttta................................................................................................................................................................................................................................................................................................................................. Subject: tfor_TF1092_TF1093|glycosyltransferase:hypothetical protein|POSITIVE:POSITIVE|[1161506,1162691]|1186 HSP 1 e-value: 1.0E-47 bit: 192.0 Len: 146 Query Start:92 Query End:237 Subject Strand: POSITIVE Subject Start: 1033 Subject End: 1178 ...........................................................................................tatttccggataaagatagcgtgtgtttgtgtggatttatgaaacggcagttaagggcaaatataccgtttatcggnnnnnnncgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaaga............................................................................................................................................................................................................................................................................................................................................................................ ...........................................................................................tatttccggataaagatagcgtgtgtttgtgtggatttatgaaacactggtcaagagcaaatataccgtttatcgggaaaaaacgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattagagcaaga............................................................................................................................................................................................................................................................................................................................................................................ Subject: tfor_TF1076_TF1077|possible glycerol kinase:conserved hypothetical protein|POSITIVE:POSITIVE|[1145742,1146521]|780 HSP 1 e-value: 3.0E-38 bit: 161.0 Len: 130 Query Start:129 Query End:258 Subject Strand: POSITIVE Subject Start: 7 Subject End: 136 ................................................................................................................................tatgaaacggcagttaagggcaaatataccgtttatcggnnnnnnncgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaagaaagggaggataattatctttg....................................................................................................................................................................................................................................................................................................................................................... ................................................................................................................................tatgaaacggcagtcaaagacaaatataccgtttatcggggaaaaacgaccgttcgttaggaaaaatgctaccgttcgttagaaaagcggagtataaattggagcaagagggagaggatgattatctttg....................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAACGAUAUAAUGAUAUAUUUUGAAAGGUAUUUCUAUGGGGCAGUGAAAGUGCGCAAUGAAUAAGUGUCUUUUCAAUCGGUCACUCGGUAUUUCCGGAUAAAGAUAGCGUGUGUUUGUGUGGAUUUAUGAAACGGCAGUUAAGGGCAAAUAUACCGUUUAUCGGAAAAAAACGACCGUUCGUUAGGAAAAAUGCUACCGUUCGUUAGAAAAGCGGAGUAUAAAUUGGAGCAAGAAAGGGAGGAUAAUUAUCUUUGCUCCCGACAUAUUAAAAUUUUACCGUGAUGAAAAAAUUAGAAAAACUAAGAUUGAAAAAAGGAAAAUCUUUUGAGGUUAUUGACUAUGGAUUGUCUAAAAGUCAAUUGUCCCAUAUGGAACAGUCUUUUCUUAUUGGAGGGGUAUAUGCUUGUGCAGGCAAUGCCUGUGGAGGUGAUGCUUGUGUAACUGAUGCAUGUGGAGGUAAUGCAUGCGGUGGUGACGGUUGUCUAAUAAACGCAUGUGGUGGAAACGCAUGUCCUGUACAAUUGUGUCCCAUUGACGCUUGUUCUGUUGAUGGUUGCUGGAUUGAUGCAUUUCAUUCAUAAGUGUAGUGUAUAAUGU .....(((.((((....((((((((((((.(((((((((.(.(((.(((......)))...(((((((((((.....(((.((.(((...((((....(((((......((((((((((((.(((((...((..((..((((((.(((((.(((((((.(((.(((........)))...(((((((((....(((((.((((((....))...))))))))).....(((((((........((((....)))))))))))..................)).)))))))..(((.(((((.....((((((...(((((.....)))))..((..(((((((.(((.....)))..)))))))...)).........))))))))))).))).))).)))))))((((.(((((((...))))))).))))..)))))...))))))..(((((((.......)))))))....))..))...))))))))))))))))).(((....))).))))).))))....))).)).))))))))))))))........))).)))))..))))).))))).....)))))))...)))).))) (-158.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACAUUAUACACUACACUUAUGAAUGAAAUGCAUCAAUCCAGCAACCAUCAACAGAACAAGCGUCAAUGGGACACAAUUGUACAGGACAUGCGUUUCCACCACAUGCGUUUAUUAGACAACCGUCACCACCGCAUGCAUUACCUCCACAUGCAUCAGUUACACAAGCAUCACCUCCACAGGCAUUGCCUGCACAAGCAUAUACCCCUCCAAUAAGAAAAGACUGUUCCAUAUGGGACAAUUGACUUUUAGACAAUCCAUAGUCAAUAACCUCAAAAGAUUUUCCUUUUUUCAAUCUUAGUUUUUCUAAUUUUUUCAUCACGGUAAAAUUUUAAUAUGUCGGGAGCAAAGAUAAUUAUCCUCCCUUUCUUGCUCCAAUUUAUACUCCGCUUUUCUAACGAACGGUAGCAUUUUUCCUAACGAACGGUCGUUUUUUUCCGAUAAACGGUAUAUUUGCCCUUAACUGCCGUUUCAUAAAUCCACACAAACACACGCUAUCUUUAUCCGGAAAUACCGAGUGACCGAUUGAAAAGACACUUAUUCAUUGCGCACUUUCACUGCCCCAUAGAAAUACCUUUCAAAAUAUAUCAUUAUAUCGUUUUUA ...............(((((....(((((((((...(((.....((((..((.........))..)))).(((....)))...))).))))))))).....((((((.......(((....))).....))))))............((((....(((.....)))..........(((((...))))).....))))...........))))).((((((.......((((((...(((((((..............)))))))...(.(((..(((.....(((((((((((.(((.....)))......((((..(((((.........((((...(((((((.((...............)))))))))....)))).((((...(((....))).((((((.........(((((....)))))..........((((((((..............)))))))).....................))))))......))))..))))).))))..))))))))))).....)))..))).)............))))))...............(((((....))))).)))))). (-107.74) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 407 530 481 186 134 tfor:TF2999|5end_conserved_hypothetical_protein_3215566..3215796_POSITIVE 383 426 382 189 141 tfor:TF3119|5end_conserved_hypothetical_protein_3352723..3352953_POSITIVE 380 122 82 230 196 tfor:TF2534|5end_conserved_hypothetical_protein_2717044..2717274_POSITIVE 379 568 528 199 158 tfor:TF0779|5end_possible_outer_membrane_protein_830346..830576_POSITIVE 377 430 383 186 142 tfor:TF1820|5end_cation_efflux_protein,_AcrB/AcrD/AcrF_family_1963363..1963593_POSITIVE 377 440 396 169 124 tfor:TF1689|5end_conserved_hypothetical_protein_1805068..1805298_POSITIVE 376 496 454 217 180 tfor:TF1947|5end_conserved_hypothetical_protein_2095521..2095751_POSITIVE 375 140 95 229 191 tfor:TF0784|5end_possible_biopolymer_transport_protein_837385..837615_POSITIVE 374 577 532 230 171 tfor:TF1252|5end_phosphofructokinase;_pyrophosphate-fructose_6-phosphate_1-phosphotransferase_(probable_beta_subunit)_1334361..1334591_POSITIVE 372 485 444 212 159 tfor:TF1974|5end_glyoxalase_I_related_protein_(lactoylglutathione_lyase)_2120950..2121180_POSITIVE 371 487 446 231 186 tfor:TF0145|5end_glutamine_synthetase_152446..152676_POSITIVE 368 459 412 82 42 tfor:TF0746|5end_possible_DNA_polymerase_III_792261..792491_POSITIVE 367 479 435 210 151 tfor:TF2520|5end_conserved_hypothetical_protein_2698917..2699147_POSITIVE 366 498 453 171 126 tfor:TF0839|5end_aminopeptidasem_M28_family_890442..890672_POSITIVE 362 159 113 169 128 tfor:TF0226|5end_hypothetical_protein_253585..253815_POSITIVE 360 437 391 56 2 tfor:TF1645|5end_conserved_hypothetical_protein_1755427..1755657_POSITIVE 360 570 524 197 146 tfor:TF0727|5end_ABC_transporter,_ATP-binding_protein_779178..779408_POSITIVE 356 510 465 199 147 tfor:TF1529|5end_hypothetical_protein_1634300..1634530_POSITIVE 354 86 41 143 104 tfor:TF1617|5end_conserved_hypothetical_protein;_possible_methyltransferase_1733150..1733380_POSITIVE 354 483 441 231 196 tfor:TF0783|5end_periplasmic_TonB_membrane-linking_protein_836552..836782_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 424 488 445 137 90 tfor:TF0815|3end_conserved_hypothetical_protein_873974..874124_POSITIVE 380 122 82 120 86 tfor:TF2533|3end_adenylosuccinate_synthetase_2717014..2717164_POSITIVE 368 459 412 49 9 tfor:TF0744|3end_DNA_polymerase_III_subunit,_gamma/tau_792308..792458_POSITIVE 362 159 113 144 103 tfor:TF0227|3end_transcriptional_regulator,_Crp/Fnr_family_253640..253790_POSITIVE 345 440 394 63 9 tfor:TF2903|3end_translation_initiation_factor_SUI1_3098986..3099136_POSITIVE 345 468 425 64 22 tfor:TF1820|3end_cation_efflux_protein,_AcrB/AcrD/AcrF_family_1966526..1966676_POSITIVE 344 564 529 137 108 tfor:TF2913|3end_conserved_hypothetical_protein_3109117..3109267_POSITIVE 342 486 449 76 37 tfor:TF1247|3end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_1330928..1331078_POSITIVE 340 437 394 69 23 tfor:TF1568|3end_H+-transporting_ATP_synthase,_subunit_D_1677038..1677188_POSITIVE 337 117 76 144 104 tfor:TF0620|3end_phosphoribosylaminoimidazole-succinocarboxamide_synthase_655735..655885_POSITIVE 336 487 446 151 106 tfor:TF2704|3end_ribonuclease_HII_2876921..2877071_POSITIVE 336 570 525 147 97 tfor:TF2149|3end_deoxyribose-phosphate_aldolase_2323110..2323260_POSITIVE 333 439 391 87 42 tfor:TF2841|3end_conserved_hypothetical_protein_3032156..3032306_POSITIVE 329 447 405 110 65 tfor:TF2500|3end_ABC_transporter,_ATP-binding_protein_2675302..2675452_POSITIVE 328 574 534 61 10 tfor:TF1480|3end_ABC_transporter,_permease_component_1574866..1575016_POSITIVE 328 499 454 150 93 tfor:TF0717|3end_signal_recognition_particle_769059..769209_POSITIVE 326 486 446 78 44 tfor:TF0668|3end_inorganic_pyrophosphatase_713235..713385_POSITIVE 325 515 474 38 1 tfor:TF0064|3end_conserved_hypothetical_protein_70412..70562_POSITIVE 324 438 403 144 112 tfor:TF2622|3end_methyltransferase_2798264..2798414_POSITIVE 323 438 394 131 67 tfor:TF2266|3end_transfer_region-related_protein,_TraQ_2434949..2435099_POSITIVE