Origin IGS: tagtcgtcccttaagaatccttttaatctctgatattgagtaaattagttttcgaaaaacctttgttaaatctgcaagttttcttatctttatagcaagaaac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gtttcttgctataaagataagaaaacttgcagatttaacaaaggtttttcgaaaactaatttactcaatatcagagattaaaaggattcttaagggacgacta Mask Tandem Repeat Region ================================================ tagtcgtcccttaagaatccttttaatctctgatattgagtaaattagttttcgaaaaacctttgttaaatctgcaagttttcttatctttatagcaagaaac Find is-nt database================================================ Query_seq: TF1075:TF1076|TF1075:TF1076:conserved hypothetical protein; possible lantibiotic biosynthesis protein:possible glycerol kinase:->->:1145462..1145564 103 tagtcgtcccttaagaatccttttaatctctgatattgagtaaattagttttcgaaaaacctttgttaaatctgcaagttttcttatctttatagcaagaaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1075:TF1076|TF1075:TF1076:conserved hypothetical protein; possible lantibiotic biosynthesis protein:possible glycerol kinase:->->:1145462..1145564 103 tagtcgtcccttaagaatccttttaatctctgatattgagtaaattagttttcgaaaaacctttgttaaatctgcaagttttcttatctttatagcaagaaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1075:TF1076|TF1075:TF1076:conserved hypothetical protein; possible lantibiotic biosynthesis protein:possible glycerol kinase:->->:1145462..1145564 103 tagtcgtcccttaagaatccttttaatctctgatattgagtaaattagttttcgaaaaacctttgttaaatctgcaagttttcttatctttatagcaagaaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagtcgtcccttaagaatccttttaatctctgatattgagtaaattagttttcgaaaaacctttgttaaatctgcaagttttcttatctttatagcaagaaac Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: - Start: 1 End: 93 L R G K L I R K I E S I S Y I L K R F V K T L D A L K R I K M .......... tagtcgtcccttaagaatccttttaatctctgatattgagtaaattagttttcgaaaaacctttgttaaatctgcaagttttcttatctttatagcaagaaac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagtcgtcccttaagaatccttttaatctctgatattgagtaaattagttttcgaaaaacctttgttaaatctgcaagttttcttatctttatagcaagaaac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1075:TF1076|TF1075:TF1076:conserved hypothetical protein; possible lantibiotic biosynthesis protein:possible glycerol kinase:->->:1145462..1145564 103 tagtcgtcccttaagaatccttttaatctctgatattgagtaaattagttttcgaaaaacctttgttaaatctgcaagttttcttatctttatagcaagaaac Intra-Species Hit: Count: 1 Min: 1 Max: 103 Len: 103 Subject: tfor_TF1075_TF1076|conserved hypothetical protein; possible lantibiotic biosynthesis protein:possible glycerol kinase|POSITIVE:POSITIVE|[1145462,1145564]|103 HSP 1 e-value: 4.0E-52 bit: 204.0 Len: 103 Query Start:1 Query End:103 Subject Strand: POSITIVE Subject Start: 1 Subject End: 103 tagtcgtcccttaagaatccttttaatctctgatattgagtaaattagttttcgaaaaacctttgttaaatctgcaagttttcttatctttatagcaagaaac tagtcgtcccttaagaatccttttaatctctgatattgagtaaattagttttcgaaaaacctttgttaaatctgcaagttttcttatctttatagcaagaaac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUCGUCCCUUAAGAAUCCUUUUAAUCUCUGAUAUUGAGUAAAUUAGUUUUCGAAAAACCUUUGUUAAAUCUGCAAGUUUUCUUAUCUUUAUAGCAAGAAAC ..............(((..((......(((.......)))......))..)))........(((((((.......(((....))).......))))))).... ( -9.14) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUUCUUGCUAUAAAGAUAAGAAAACUUGCAGAUUUAACAAAGGUUUUUCGAAAACUAAUUUACUCAAUAUCAGAGAUUAAAAGGAUUCUUAAGGGACGACUA ((..((((............((((((((.............)))))))).(((..((......(((.......)))......))..))).))))..))..... (-13.62) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 256 100 52 74 22 tfor:TF2154|5end_conserved_hypothetical_protein_2329153..2329383_POSITIVE 244 45 6 63 22 tfor:TF2686|5end_ABC_transporter,_ATP-binding_protein_2862741..2862971_POSITIVE 242 45 8 229 188 tfor:TF2664|5end_hypothetical_protein_2841399..2841629_POSITIVE 214 98 53 226 175 tfor:TF2321|5end_hypothetical_protein_2482347..2482577_POSITIVE 209 100 51 152 103 tfor:TF2771|5end_undecaprenol_kinase,_bacitracin_resistance_protein_2956559..2956789_POSITIVE 208 39 4 54 12 tfor:TF0107|5end_ion_channel_protein_113913..114143_POSITIVE 207 88 47 88 29 tfor:TF0814|5end_conserved_hypothetical_protein_872546..872776_POSITIVE 205 75 31 227 189 tfor:TF2947|5end_isochorismate_synthase_3153633..3153863_POSITIVE 201 54 16 115 77 tfor:TF1902|5end_two-component_system_sensor_histidine_kinase_2057521..2057751_POSITIVE 199 90 43 201 160 tfor:TF1875|5end_conserved_hypothetical_protein_2028752..2028982_POSITIVE 197 40 5 231 192 tfor:TF2950|5end_chloromuconate_cycloisomerase_3157399..3157629_POSITIVE 197 90 49 171 122 tfor:TF1490|5end_hypothetical_protein_1590238..1590468_POSITIVE 193 29 2 156 135 tfor:TF2411|5end_outer_membrane_protein_2585451..2585681_POSITIVE 190 84 49 217 178 tfor:TF0976|5end_outer_membrane_receptor,_Ton-linked_1024482..1024712_POSITIVE 188 85 61 191 164 tfor:TF1613|5end_transposase_1730963..1731193_POSITIVE 188 38 3 45 10 tfor:TF1181|5end_methylated-DNA--[protein]-cysteine_S-methyltransferase_1267522..1267752_POSITIVE 187 76 31 223 180 tfor:TF2453|5end_glutamate_racemase_2630422..2630652_POSITIVE 187 50 5 219 168 tfor:TF2356|5end_PAP2_superfamily_protein_2520800..2521030_POSITIVE 187 45 5 103 65 tfor:TF1573|5end_ATP_synthase,_subunit_E_1680955..1681185_POSITIVE 184 96 52 161 107 tfor:TF0990|5end_hypothetical_protein_1045395..1045625_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 242 45 8 73 32 tfor:TF2663|3end_hypothetical_protein_2841323..2841473_POSITIVE 227 93 59 69 34 tfor:TF2802|3end_possible_outer_membrane_protein_2991814..2991964_POSITIVE 214 93 53 113 75 tfor:TF2657|3end_conserved_hypothetical_protein_2830873..2831023_POSITIVE 207 96 51 147 103 tfor:TF2770|3end_conserved_hypothetical_protein_2956639..2956789_POSITIVE 207 88 47 69 10 tfor:TF0812|3end_conserved_hypothetical_protein_872607..872757_POSITIVE 201 54 16 141 103 tfor:TF1903|3end_hypothetical_protein_2057627..2057777_POSITIVE 193 29 2 57 36 tfor:TF2410|3end_possible_endo-1,4-beta-xylanase_2585432..2585582_POSITIVE 187 76 31 136 93 tfor:TF2452|3end_outer_membrane_protein,_OmpH_family_2630415..2630565_POSITIVE 187 45 5 100 62 tfor:TF1571|3end_conserved_hypothetical_protein_1681032..1681182_POSITIVE 184 37 2 75 36 tfor:TF2349|3end_conserved_hypothetical_protein_2514921..2515071_POSITIVE 183 33 1 81 49 tfor:TF1698|3end_mobilization_protein_1819782..1819932_POSITIVE 182 75 31 142 107 tfor:TF0403|3end_TPR_repeat_protein_423483..423633_POSITIVE 180 42 6 101 66 tfor:TF1597|3end_TatD_family_protein_1713418..1713568_POSITIVE 179 43 4 75 35 tfor:TF2647|3end_transcriptional_regulator,_possible_arylsulfatase_regulator_2818135..2818285_POSITIVE 177 42 2 113 68 tfor:TF2119|3end_conserved_hypothetical_protein_2292188..2292338_POSITIVE 175 33 5 78 45 tfor:TF0556|3end_Bacteroides_aerotolerance_protein,_BatA_589149..589299_POSITIVE 174 44 4 128 84 tfor:TF2278|3end_conserved_hypothetical_protein_2438990..2439140_POSITIVE 173 39 5 35 2 tfor:TF2340|3end_hypothetical_protein_2500915..2501065_POSITIVE 172 42 18 133 107 tfor:TF1804|3end_hypothetical_protein_1947957..1948107_POSITIVE 171 90 43 137 94 tfor:TF1807|3end_thioesterase_family_protein_1949425..1949575_POSITIVE