Origin IGS:
gttaaattcatgttaaatgttttcttcgggcaaaggtatacaattctataatcaacggaacgtatatcgttcttttttttgagtggcctttccatattcgatgactcggacaggacaatgatggatacataccagacattgtgtgcaatttctgccccagtgcggctttttatcgaccagtgcaatattcgcggtaagacactgtgaaacacaaagggagaagccttatcatccacgtcgaatctggaaaaaatcaccta
.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9
taggtgattttttccagattcgacgtggatgataaggcttctccctttgtgtttcacagtgtcttaccgcgaatattgcactggtcgataaaaagccgcactggggcagaaattgcacacaatgtctggtatgtatccatcattgtcctgtccgagtcatcgaatatggaaaggccactcaaaaaaaagaacgatatacgttccgttgattatagaattgtatacctttgcccgaagaaaacatttaacatgaatttaac

Mask Tandem Repeat Region ================================================
gttaaattcatgttaaatgttttcttcgggcaaaggtatacaattctataatcaacggaacgtatatcgttcttttttttgagtggcctttccatattcgatgactcggacaggacaatgatggatacataccagacattgtgtgcaatttctgccccagtgcggctttttatcgaccagtgcaatattcgcggtaagacactgtgaaacacaaagggagaagccttatcatccacgtcgaatctggaaaaaatcaccta

Find is-nt database================================================
Query_seq: TF1033:TF1034|TF1033:TF1034:endothelin converting enzyme, endopeptidase:conserved hypothetical protein:->->:1094943..1095202 260
gttaaattcatgttaaatgttttcttcgggcaaaggtatacaattctataatcaacggaacgtatatcgttcttttttttgagtggcctttccatattcgatgactcggacaggacaatgatggatacataccagacattgtgtgcaatttctgccccagtgcggctttttatcgaccagtgcaatattcgcggtaagacactgtgaaacacaaagggagaagccttatcatccacgtcgaatctggaaaaaatcaccta
Intra-Species Hit: Count: 0
Inter-species Hit: Count: 0

Find is-aa database================================================
Query_seq: TF1033:TF1034|TF1033:TF1034:endothelin converting enzyme, endopeptidase:conserved hypothetical protein:->->:1094943..1095202 260
gttaaattcatgttaaatgttttcttcgggcaaaggtatacaattctataatcaacggaacgtatatcgttcttttttttgagtggcctttccatattcgatgactcggacaggacaatgatggatacataccagacattgtgtgcaatttctgccccagtgcggctttttatcgaccagtgcaatattcgcggtaagacactgtgaaacacaaagggagaagccttatcatccacgtcgaatctggaaaaaatcaccta
Intra-Species Hit: Count: 0
Inter-species Hit: Count: 0

Find nr database================================================
Query_seq: TF1033:TF1034|TF1033:TF1034:endothelin converting enzyme, endopeptidase:conserved hypothetical protein:->->:1094943..1095202 260
gttaaattcatgttaaatgttttcttcgggcaaaggtatacaattctataatcaacggaacgtatatcgttcttttttttgagtggcctttccatattcgatgactcggacaggacaatgatggatacataccagacattgtgtgcaatttctgccccagtgcggctttttatcgaccagtgcaatattcgcggtaagacactgtgaaacacaaagggagaagccttatcatccacgtcgaatctggaaaaaatcaccta
Intra-Species Hit: Count: 0

Inter-species Hit: Count: 14	Min: 61	Max: 225	Len: 165
Subject: UniRef90_A0UW66 Cluster: 4Fe-4S ferredoxin, iron-sulfur binding; n=1; Clostridium cellulolyticum H10|Rep: 4Fe-4S ferredoxin, iron-sulfur binding - Clostridium cellulolyticum H10
HSP  1	e-value: 1.0E-10	bit: 67.4	Len: 165	Query Start:61	Query End:225	Subject Strand: null	Subject Start: 196	Subject End: 249
............................................................ G  F  S  L  C  V  S  Q  C  L  T  A  N  I  A  L  V  D  K  K  P  H  W  G  R  N  C  T  Q  C  L  V  C  I  H  H  C  P  V  R  V  I  E  Y  G  K  A  T  Q  K  K  E  R  Y  T ...................................
............................................................ G  C  G  I  C  E  K  V  C  T  S  K  S  I  K  -  V  D  K  R  P  K  W  G  K  E  C  T  Q  C  L  A  C  I  N  F  C  P  T  K  A  T  Q  F  G  K  G  T  E  K  K  G  R  Y  T ...................................

Subject: UniRef90_Q1FKB9 Cluster: 4Fe-4S ferredoxin, iron-sulfur binding; n=1; Clostridium phytofermentans ISDg|Rep: 4Fe-4S ferredoxin, iron-sulfur binding - Clostridium phytofermentans ISDg
HSP  1	e-value: 2.0E-10	bit: 67.0	Len: 150	Query Start:64	Query End:213	Subject Strand: null	Subject Start: 195	Subject End: 244
............................................................... C  V  S  Q  C  L  T  A  N  I  A  L  V  D  K  K  P  H  W  G  R  N  C  T  Q  C  L  V  C  I  H  H  C  P  V  R  V  I  E  Y  G  K  A  T  Q  K  K  E  R  Y ...............................................
............................................................... C  V  R  S  C  P  L  N  N  I  Q  L  E  D  K  K  P  V  W  G  K  D  C  T  H  C  M  A  C  I  C  G  C  P  I  K  A  I  E  Y  G  K  N  S  K  S  K  V  R  Y ...............................................

Subject: UniRef90_Q8AAN8 Cluster: Hypothetical protein; n=1; Bacteroides thetaiotaomicron|Rep: Hypothetical protein - Bacteroides thetaiotaomicron
HSP  1	e-value: 6.0E-9	bit: 62.0	Len: 162	Query Start:64	Query End:225	Subject Strand: null	Subject Start: 196	Subject End: 249
............................................................... G  F  S  L  C  V  S  Q  C  L  T  A  N  I  A  L  V  D  K  K  P  H  W  G  R  N  C  T  Q  C  L  V  C  I  H  H  C  P  V  R  V  I  E  Y  G  K  A  T  Q  K  K  E  R  Y ...................................
............................................................... G  C  K  R  C  E  K  S  C  P  V  G  N  I  T  M  K  E  R  R  P  V  W  G  K  N  C  T  A  C  L  A  C  Y  H  V  C  P  Q  H  A  V  Q  Y  G  K  K  T  K  G  K  G  H  Y ...................................

Subject: UniRef90_A0V1U2 Cluster: 4Fe-4S ferredoxin, iron-sulfur binding; n=1; Clostridium cellulolyticum H10|Rep: 4Fe-4S ferredoxin, iron-sulfur binding - Clostridium cellulolyticum H10
HSP  1	e-value: 8.0E-9	bit: 61.6	Len: 162	Query Start:64	Query End:225	Subject Strand: null	Subject Start: 191	Subject End: 244
............................................................... G  F  S  L  C  V  S  Q  C  L  T  A  N  I  A  L  V  D  K  K  P  H  W  G  R  N  C  T  Q  C  L  V  C  I  H  H  C  P  V  R  V  I  E  Y  G  K  A  T  Q  K  K  E  R  Y ...................................
............................................................... G  C  G  I  C  K  K  V  C  H  L  N  N  I  E  M  I  N  K  R  P  H  W  G  N  E  C  S  T  C  L  A  C  F  H  W  C  P  K  K  A  V  K  G  G  K  M  L  N  K  R  G  R  Y ...................................

Subject: UniRef90_Q184V9 Cluster: Putative flavodoxin; n=2; Clostridium difficile|Rep: Putative flavodoxin - Clostridium difficile (strain 630)
HSP  1	e-value: 1.0E-8	bit: 61.2	Len: 162	Query Start:64	Query End:225	Subject Strand: null	Subject Start: 193	Subject End: 246
............................................................... G  F  S  L  C  V  S  Q  C  L  T  A  N  I  A  L  V  D  K  K  P  H  W  G  R  N  C  T  Q  C  L  V  C  I  H  H  C  P  V  R  V  I  E  Y  G  K  A  T  Q  K  K  E  R  Y ...................................
............................................................... G  C  G  K  C  V  E  L  C  P  L  N  N  I  N  L  K  N  K  K  P  V  W  K  N  N  C  T  H  C  M  A  C  I  C  G  C  P  T  E  A  I  E  Y  K  N  K  T  Q  N  R  E  R  Y ...................................

Subject: UniRef90_Q2WK06 Cluster: Hypothetical protein; n=1; Clostridium beijerincki NCIMB 8052|Rep: Hypothetical protein - Clostridium beijerincki NCIMB 8052
HSP  1	e-value: 4.0E-8	bit: 59.3	Len: 162	Query Start:64	Query End:225	Subject Strand: null	Subject Start: 189	Subject End: 242
............................................................... G  F  S  L  C  V  S  Q  C  L  T  A  N  I  A  L  V  D  K  K  P  H  W  G  R  N  C  T  Q  C  L  V  C  I  H  H  C  P  V  R  V  I  E  Y  G  K  A  T  Q  K  K  E  R  Y ...................................
............................................................... G  C  G  I  C  S  N  V  C  P  A  E  N  I  E  I  I  E  A  K  P  S  W  E  H  R  C  E  Q  C  L  A  C  I  H  L  C  P  Q  T  A  I  E  F  K  K  D  S  I  N  K  E  R  Y ...................................

Subject: UniRef90_Q64VN8 Cluster: Hypothetical protein; n=2; Bacteroides fragilis|Rep: Hypothetical protein - Bacteroides fragilis
HSP  1	e-value: 2.0E-7	bit: 57.0	Len: 162	Query Start:64	Query End:225	Subject Strand: null	Subject Start: 196	Subject End: 249
............................................................... G  F  S  L  C  V  S  Q  C  L  T  A  N  I  A  L  V  D  K  K  P  H  W  G  R  N  C  T  Q  C  L  V  C  I  H  H  C  P  V  R  V  I  E  Y  G  K  A  T  Q  K  K  E  R  Y ...................................
............................................................... G  C  K  R  C  E  R  I  C  P  V  G  N  V  V  M  I  G  W  R  P  V  W  G  M  D  C  T  S  C  L  A  C  Y  H  V  C  P  K  H  A  V  Q  Y  G  R  R  T  K  R  K  G  Q  Y ...................................

Subject: UniRef90_A3CUF6 Cluster: 4Fe-4S ferredoxin, iron-sulfur binding domain protein; n=1; Methanoculleus marisnigri JR1|Rep: 4Fe-4S ferredoxin, iron-sulfur binding domain protein - Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
HSP  1	e-value: 2.0E-7	bit: 57.0	Len: 153	Query Start:64	Query End:216	Subject Strand: null	Subject Start: 199	Subject End: 249
............................................................... L  C  V  S  Q  C  L  T  A  N  I  A  L  V  D  K  K  P  H  W  G  R  N  C  T  Q  C  L  V  C  I  H  H  C  P  V  R  V  I  E  Y  G  K  A  T  Q  K  K  E  R  Y ............................................
............................................................... I  C  A  S  I  C  P  A  E  N  I  E  M  V  D  G  R  P  V  W  N  H  R  C  E  L  C  C  G  C  I  H  L  C  P  A  G  A  I  Q  A  G  K  A  T  E  G  R  Q  R  Y ............................................

Subject: UniRef90_Q0W7Z7 Cluster: 2(4Fe-4S) ferredoxin-domain protein; n=1; uncultured methanogenic archaeon RC-I|Rep: 2(4Fe-4S) ferredoxin-domain protein - Uncultured methanogenic archaeon RC-I
HSP  1	e-value: 6.0E-7	bit: 55.5	Len: 150	Query Start:64	Query End:213	Subject Strand: null	Subject Start: 84	Subject End: 133
............................................................... C  V  S  Q  C  L  T  A  N  I  A  L  V  D  K  K  P  H  W  G  R  N  C  T  Q  C  L  V  C  I  H  H  C  P  V  R  V  I  E  Y  G  K  A  T  Q  K  K  E  R  Y ...............................................
............................................................... C  V  K  V  C  P  V  N  N  V  T  L  T  D  R  K  V  T  W  G  P  N  C  I  H  C  L  A  C  F  H  W  C  P  A  R  A  V  E  I  G  G  K  S  A  D  I  A  R  Y ...............................................

Subject: UniRef90_Q0TNL6 Cluster: Iron-sulfur cluster-binding protein; n=3; Clostridium perfringens|Rep: Iron-sulfur cluster-binding protein - Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A)
HSP  1	e-value: 8.0E-7	bit: 55.1	Len: 153	Query Start:64	Query End:216	Subject Strand: null	Subject Start: 196	Subject End: 246
............................................................... L  C  V  S  Q  C  L  T  A  N  I  A  L  V  D  K  K  P  H  W  G  R  N  C  T  Q  C  L  V  C  I  H  H  C  P  V  R  V  I  E  Y  G  K  A  T  Q  K  K  E  R  Y ............................................
............................................................... I  C  A  K  I  C  P  N  K  N  I  E  I  I  D  E  G  P  K  W  K  G  N  C  C  D  C  M  G  C  V  N  N  C  P  H  K  C  I  N  I  G  N  K  T  K  K  K  N  R  Y ............................................

Subject: UniRef90_Q2WP37 Cluster: Hypothetical protein; n=1; Clostridium beijerincki NCIMB 8052|Rep: Hypothetical protein - Clostridium beijerincki NCIMB 8052
HSP  1	e-value: 8.0E-7	bit: 55.1	Len: 165	Query Start:61	Query End:225	Subject Strand: null	Subject Start: 189	Subject End: 243
............................................................ G  F  S  L  C  V  S  Q  C  L  T  A  N  I  A  L  V  D  K  K  P  H  W  G  R  N  C  T  Q  C  L  V  C  I  H  H  C  P  V  R  V  I  E  Y  G  K  A  T  Q  K  K  E  R  Y  T ...................................
............................................................ G  C  R  T  C  E  A  V  C  P  V  S  N  I  V  M  K  N  K  K  P  S  F  K  H  N  C  E  Q  C  M  A  C  V  Q  W  C  P  K  Q  A  I  N  Y  K  N  K  T  Q  S  R  G  R  Y  T ...................................

Subject: UniRef90_Q2DT94 Cluster: Hypothetical protein; n=1; Geobacter uraniumreducens Rf4|Rep: Hypothetical protein - Geobacter uraniumreducens Rf4
HSP  1	e-value: 2.0E-6	bit: 53.9	Len: 153	Query Start:64	Query End:216	Subject Strand: null	Subject Start: 196	Subject End: 246
............................................................... L  C  V  S  Q  C  L  T  A  N  I  A  L  V  D  K  K  P  H  W  G  R  N  C  T  Q  C  L  V  C  I  H  H  C  P  V  R  V  I  E  Y  G  K  A  T  Q  K  K  E  R  Y ............................................
............................................................... I  C  E  R  A  C  P  V  K  N  I  T  L  E  A  G  R  P  L  W  H  G  S  C  E  Q  C  L  A  C  I  Q  W  C  P  E  E  C  I  Q  Y  G  K  K  T  K  N  Y  E  R  Y ............................................

Subject: UniRef90_Q188R5 Cluster: Putative iron-sulfur protein; n=3; Clostridium difficile|Rep: Putative iron-sulfur protein - Clostridium difficile (strain 630)
HSP  1	e-value: 3.0E-6	bit: 53.1	Len: 141	Query Start:85	Query End:225	Subject Strand: null	Subject Start: 191	Subject End: 237
.................................................................................... G  F  S  L  C  V  S  Q  C  L  T  A  N  I  A  L  V  D  K  K  P  H  W  G  R  N  C  T  Q  C  L  V  C  I  H  H  C  P  V  R  V  I  E  Y  G  K  A ...................................
.................................................................................... G  C  G  T  C  K  R  V  C  P  V  E  N  I  S  I  V  D  K  K  P  K  W  G  N  E  C  E  R  C  L  A  C  F  H  W  C  P  K  E  A  I  N  V  K  K  S ...................................

Subject: UniRef90_Q2WTV7 Cluster: Hypothetical protein; n=1; Clostridium beijerincki NCIMB 8052|Rep: Hypothetical protein - Clostridium beijerincki NCIMB 8052
HSP  1	e-value: 4.0E-6	bit: 52.8	Len: 156	Query Start:64	Query End:219	Subject Strand: null	Subject Start: 190	Subject End: 241
............................................................... S  L  C  V  S  Q  C  L  T  A  N  I  A  L  V  D  K  K  P  H  W  G  R  N  C  T  Q  C  L  V  C  I  H  H  C  P  V  R  V  I  E  Y  G  K  A  T  Q  K  K  E  R  Y .........................................
............................................................... N  M  C  K  K  V  C  P  V  D  N  I  V  I  E  N  N  R  P  K  W  L  G  K  C  T  D  C  M  A  C  I  N  I  C  P  K  E  A  I  N  I  G  K  S  T  I  K  K  N  R  Y .........................................


gttaaattcatgttaaatgttttcttcgggcaaaggtatacaattctataatcaacggaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttatcatccacgtcgaatctggaaaaaatcaccta
Predict ORF larger than 30AA ================================================
Protein_Len: 32	Strand: +	Start: 6	End: 101
..... M  H  V  K  C  F  L  R  A  K  V  Y  N  S  I  I  N  G  T  Y  I  V  L  F  F  E  W  P  F  H  I  R ...............................................................................................................................................................
Protein_Len: 47	Strand: +	Start: 64	End: 204
............................................................... M  S  F  F  F  L  S  G  L  S  I  F  D  D  S  D  R  T  M  M  D  T  Y  Q  T  L  C  A  I  S  A  P  V  R  L  F  I  D  Q  C  N  I  R  G  K  T  L ........................................................
Protein_Len: 45	Strand: +	Start: 125	End: 259
............................................................................................................................ M  H  T  R  H  C  V  Q  F  L  P  Q  C  G  F  L  S  T  S  A  I  F  A  V  R  H  C  E  T  Q  R  E  K  P  Y  H  P  R  R  I  W  K  K  S  P .
Protein_Len: 44	Strand: -	Start: 55	End: 186
...................................................... R  F  T  Y  R  E  K  K  Q  T  A  K  G  Y  E  I  V  R  V  P  C  H  H  I  C  V  L  C  Q  T  C  N  R  G  W  H  P  K  K  D  V  L  A  M ..........................................................................
Protein_Len: 50	Strand: -	Start: 11	End: 160
.......... T  L  H  K  R  R  A  F  T  Y  L  E  I  I  L  P  V  Y  I  T  R  K  K  S  H  G  K  W  I  R  H  S  P  C  S  L  S  P  Y  M  G  S  M  T  H  L  K  Q  G  M ....................................................................................................
Protein_Len: 60	Strand: -	Start: 3	End: 257
.. K  L  P  R  E  M  N  S  S  E  S  L  V  I  I  S  V  Y  W  V  N  H  A  I  E  A  G  T  R  S  K  I  S  W  H  L  I  R  P  L  V  S  H  F  V  F  P  S  A  K  D  D  V  D  F  R  S  F  I  M ...

gttaaattcatgttaaatgttttcttcgggcaaaggtatacaattctataatcaacggaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttatcatccacgtcgaatctggaaaaaatcaccta
Predict Promoter with matrix: RpoD-15 score > 80================================================

Predict Promoter with matrix: RpoD-16 score > 80================================================

Predict Promoter with matrix: RpoD-17 score > 80================================================

Predict Promoter with matrix: RpoD-18 score > 80================================================

Predict Promoter with matrix: RpoD-19 score > 80================================================

Predict Promoter with matrix: RpoN score > 80================================================

gttaaattcatgttaaatgttttcttcgggcaaaggtatacaattctataatcaacggaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttatcatccacgtcgaatctggaaaaaatcaccta
Predict TransTerm conf > 70================================================
TransTerm Strand: -	Conf: 40	HP_score: -2.2	Tail_Score: -2.67017	Start: 57	End: 73	Full_Region: ggccactcaaaaaaa agaacg atata cgttcc gttgattatagaatt
........................................................agaacgatatacgttcc...........................................................................................................................................................................................
TransTerm Strand: +	Conf: 100	HP_score: -5.7	Tail_Score: -5.81412	Start: 58	End: 72	Full_Region: ATTCTATAATCAACG GAACG TATAT CGTTC TTTTTTTTGAGTGGC
.........................................................GAACGTATATCGTTC............................................................................................................................................................................................
TransTerm Strand: +	Conf: 72	HP_score: -4.7	Tail_Score: -4.83169	Start: 154	End: 166	Full_Region: TTGTGTGCAATTTCT GCCCC AGT GCGGC TTTTTATCGACCAGT
.........................................................................................................................................................GCCCCAGTGCGGC..............................................................................................

Find igs database================================================
Query_seq: TF1033:TF1034|TF1033:TF1034:endothelin converting enzyme, endopeptidase:conserved hypothetical protein:->->:1094943..1095202 260
gttaaattcatgttaaatgttttcttcgggcaaaggtatacaattctataatcaacggaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttatcatccacgtcgaatctggaaaaaatcaccta
Intra-Species Hit: Count: 1	Min: 1	Max: 260	Len: 260
Subject: tfor_TF1033_TF1034|endothelin converting enzyme, endopeptidase:conserved hypothetical protein|POSITIVE:POSITIVE|[1094943,1095202]|260
HSP  1	e-value: 5.0E-26	bit: 119.0	Len: 60	Query Start:1	Query End:60	Subject Strand: POSITIVE	Subject Start: 1	Subject End: 60
gttaaattcatgttaaatgttttcttcgggcaaaggtatacaattctataatcaacggaa........................................................................................................................................................................................................
gttaaattcatgttaaatgttttcttcgggcaaaggtatacaattctataatcaacggaa........................................................................................................................................................................................................
HSP  2	e-value: 4.0E-11	bit: 69.9	Len: 35	Query Start:226	Query End:260	Subject Strand: POSITIVE	Subject Start: 226	Subject End: 260
.................................................................................................................................................................................................................................ttatcatccacgtcgaatctggaaaaaatcaccta
.................................................................................................................................................................................................................................ttatcatccacgtcgaatctggaaaaaatcaccta

Inter-species Hit: Count: 0

Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================
GUUAAAUUCAUGUUAAAUGUUUUCUUCGGGCAAAGGUAUACAAUUCUAUAAUCAACGGAACGUAUAUCGUUCUUUUUUUUGAGUGGCCUUUCCAUAUUCGAUGACUCGGACAGGACAAUGAUGGAUACAUACCAGACAUUGUGUGCAAUUUCUGCCCCAGUGCGGCUUUUUAUCGACCAGUGCAAUAUUCGCGGUAAGACACUGUGAAACACAAAGGGAGAAGCCUUAUCAUCCACGUCGAAUCUGGAAAAAAUCACCUA
..........(((...((.(((((....)).))).))..)))..............((((((.....)))))).........((((..((((((.(((((((((((.((.((((((((((.(((.......)))..))))))........)))).)).)))..((((((((.....((..((.....((((((((.....))))))))...))..))))))))))..........)))))))).))))))...))))... (-60.00)

Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================
UAGGUGAUUUUUUCCAGAUUCGACGUGGAUGAUAAGGCUUCUCCCUUUGUGUUUCACAGUGUCUUACCGCGAAUAUUGCACUGGUCGAUAAAAAGCCGCACUGGGGCAGAAAUUGCACACAAUGUCUGGUAUGUAUCCAUCAUUGUCCUGUCCGAGUCAUCGAAUAUGGAAAGGCCACUCAAAAAAAAGAACGAUAUACGUUCCGUUGAUUAUAGAAUUGUAUACCUUUGCCCGAAGAAAACAUUUAACAUGAAUUUAAC
((((((.(((((((.....((((((.(((((....(((((.(((((((.((((...((((((..((.......))..))))))...)))).))))..(.(((.((((((.........((((((..(((.......))).)))))).)))))).)))).........))).)))))..((........))........)))))))))))..........(((......)))..))))))).))))))............. (-58.00)

Find mRNA Target Using  Conserved IGS================================================
5'END mRNA Target Prediction===================================
Score	srna_start	srna_end	target_start	tegart_end	seq_id
376	167	123	168	127	tfor:TF2357|5end_collagenase_2522561..2522791_POSITIVE
376	209	161	213	166	tfor:TF0813|5end_glycosyl_hydrolase,_secreted_870677..870907_POSITIVE
365	239	191	162	105	tfor:TF1919|5end_hypothetical_protein_2070037..2070267_POSITIVE
365	239	191	122	65	tfor:TF1917|5end_hypothetical_protein_2069997..2070227_POSITIVE
344	194	151	51	10	tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE
335	115	75	165	120	tfor:TF2831|5end_ABC_transporter,_permease_component_3021317..3021547_POSITIVE
331	178	134	199	158	tfor:TF2918|5end_hypothetical_protein_3114084..3114314_POSITIVE
331	130	82	153	107	tfor:TF1924|5end_prenyltransferase,_UbiA_family_2072857..2073087_POSITIVE
331	63	21	89	45	tfor:TF0755|5end_conserved_hypothetical_protein_802763..802993_POSITIVE
328	166	127	65	26	tfor:TF3028|5end_1-deoxy-d-xylulose-5-phosphate_reductoisomerase_3251642..3251872_POSITIVE
326	198	151	189	137	tfor:TF1043|5end_conserved_hypothetical_protein;_possible_hydrolase_1105282..1105512_POSITIVE
320	238	192	196	155	tfor:TF0195|5end_outer_membrane_protein_215631..215861_POSITIVE
319	195	154	50	9	tfor:TF0777|5end_1,4-dihydroxy-2-naphthoate_octaprenyltransferase_826098..826328_POSITIVE
318	194	152	221	172	tfor:TF1933|5end_methionyl-tRNA_synthetase_2079675..2079905_POSITIVE
318	194	154	53	16	tfor:TF0951|5end_D-alanine--D-alanine_ligase_995718..995948_POSITIVE
318	199	154	220	160	tfor:TF0464|5end_precorrin_methylase_480211..480441_POSITIVE
316	200	154	202	159	tfor:TF2562|5end_50S_ribosomal_protein_L29_2739133..2739363_POSITIVE
316	208	162	222	179	tfor:TF0937|5end_ATPase,__AAA+_class_978390..978620_POSITIVE
315	196	152	219	166	tfor:TF1466|5end_DNA_gyrase_modulator_1555469..1555699_POSITIVE
311	109	61	223	167	tfor:TF0684|5end_conserved_hypothetical_protein_728853..729083_POSITIVE

3'END mRNA Target Prediction===================================
Score	srna_start	srna_end	target_start	tegart_end	seq_id
365	239	191	150	93	tfor:TF1918|3end_hypothetical_protein_2070105..2070255_POSITIVE
355	110	61	144	78	tfor:TF3115|3end_possible_sugar_kinase_3352025..3352175_POSITIVE
354	200	151	60	14	tfor:TF2953|3end_possible_TonB-dependent_outer_membrane_receptor_3161501..3161651_POSITIVE
331	130	82	145	99	tfor:TF1923|3end_conserved_hypothetical_protein;_possible_phosphoserine_phosphatase_2072929..2073079_POSITIVE
326	198	151	122	70	tfor:TF1042|3end_possible_surface_antigen_with_leucine-rich_repeat_1105295..1105445_POSITIVE
320	238	192	141	100	tfor:TF0194|3end_hypothetical_protein_215656..215806_POSITIVE
319	195	154	45	4	tfor:TF0776|3end_ABC_transporter,_permease_component_826173..826323_POSITIVE
315	194	151	119	77	tfor:TF2547|3end_hypothetical_protein_2730947..2731097_POSITIVE
313	196	152	114	76	tfor:TF0145|3end_glutamine_synthetase_154718..154868_POSITIVE
311	166	124	53	20	tfor:TF0037|3end_sialic_acid-specific_9-O-acetylesterase_39991..40141_POSITIVE
311	167	121	43	4	tfor:TF0023|3end_possible_response_element_in_two_component_regulation_20233..20383_POSITIVE
309	196	154	99	46	tfor:TF0877|3end_ISPg2-related_(PGIS2-related)_transposase_931817..931967_POSITIVE
308	184	142	47	1	tfor:TF0582|3end_hemolysin-related_protein,_with_CBS_domains;_possible_gliding-motility_related_protein_613263..613413_POSITIVE
306	178	132	49	3	tfor:TF3151|3end_glyoxalase_family_protein_3386705..3386855_POSITIVE
306	195	159	67	16	tfor:TF0464|3end_precorrin_methylase_482108..482258_POSITIVE
302	195	154	71	25	tfor:TF1656|3end_possible_2-methylthioadenine_synthetase_1772505..1772655_POSITIVE
296	188	156	46	19	tfor:TF0275|3end_outer_membrane_protein_303990..304140_POSITIVE
295	169	121	66	5	tfor:TF0157|3end_conserved_hypothetical_protein_167528..167678_POSITIVE
293	194	154	81	35	tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE
293	196	151	45	7	tfor:TF0214|3end_DNA_recombination_and_repair_protein,_RecN_241571..241721_POSITIVE