Origin IGS: ggtttgatattttaattattattacatgctaattttaagcacaaataaaggaataattctttgattcgaaaaatatttcggcaaaaaaatgatgaattgaatcaaataaccttttatat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atataaaaggttatttgattcaattcatcatttttttgccgaaatatttttcgaatcaaagaattattcctttatttgtgcttaaaattagcatgtaataataattaaaatatcaaacc Mask Tandem Repeat Region ================================================ ggtttgatattttaattattattacatgctaattttaagcacaaataaaggaataattctttgattcgaaaaatatttcggcaaaaaaatgatgaattgaatcaaataaccttttatat Find is-nt database================================================ Query_seq: TF1027:TF1028|TF1027:TF1028:hypothetical protein:1-acyl-sn-glycerol-3-phosphate acyltransferase:->->:1085958..1086076 119 ggtttgatattttaattattattacatgctaattttaagcacaaataaaggaataattctttgattcgaaaaatatttcggcaaaaaaatgatgaattgaatcaaataaccttttatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1027:TF1028|TF1027:TF1028:hypothetical protein:1-acyl-sn-glycerol-3-phosphate acyltransferase:->->:1085958..1086076 119 ggtttgatattttaattattattacatgctaattttaagcacaaataaaggaataattctttgattcgaaaaatatttcggcaaaaaaatgatgaattgaatcaaataaccttttatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1027:TF1028|TF1027:TF1028:hypothetical protein:1-acyl-sn-glycerol-3-phosphate acyltransferase:->->:1085958..1086076 119 ggtttgatattttaattattattacatgctaattttaagcacaaataaaggaataattctttgattcgaaaaatatttcggcaaaaaaatgatgaattgaatcaaataaccttttatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ggtttgatattttaattattattacatgctaattttaagcacaaataaaggaataattctttgattcgaaaaatatttcggcaaaaaaatgatgaattgaatcaaataaccttttatat Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: - Start: 25 End: 117 ........................ M S I K L V F L P I I R Q N S F Y K P L F I I F Q I L Y G K M .. ggtttgatattttaattattattacatgctaattttaagcacaaataaaggaataattctttgattcgaaaaatatttcggcaaaaaaatgatgaattgaatcaaataaccttttatat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ggtttgatattttaattattattacatgctaattttaagcacaaataaaggaataattctttgattcgaaaaatatttcggcaaaaaaatgatgaattgaatcaaataaccttttatat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1027:TF1028|TF1027:TF1028:hypothetical protein:1-acyl-sn-glycerol-3-phosphate acyltransferase:->->:1085958..1086076 119 ggtttgatattttaattattattacatgctaattttaagcacaaataaaggaataattctttgattcgaaaaatatttcggcaaaaaaatgatgaattgaatcaaataaccttttatat Intra-Species Hit: Count: 1 Min: 1 Max: 119 Len: 119 Subject: tfor_TF1027_TF1028|hypothetical protein:1-acyl-sn-glycerol-3-phosphate acyltransferase|POSITIVE:POSITIVE|[1085958,1086076]|119 HSP 1 e-value: 4.0E-49 bit: 194.0 Len: 119 Query Start:1 Query End:119 Subject Strand: POSITIVE Subject Start: 1 Subject End: 119 ggtttgatattttaattattattacatgctaattttaagcacaaataaaggaataattctttgattcgaaaaatatttcggcnnnnnnntgatgaattgaatcaaataaccttttatat ggtttgatattttaattattattacatgctaattttaagcacaaataaaggaataattctttgattcgaaaaatatttcggcaaaaaaatgatgaattgaatcaaataaccttttatat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUUUGAUAUUUUAAUUAUUAUUACAUGCUAAUUUUAAGCACAAAUAAAGGAAUAAUUCUUUGAUUCGAAAAAUAUUUCGGCAAAAAAAUGAUGAAUUGAAUCAAAUAACCUUUUAUAU ((((......(((((((((((((...((((.......))))....(((((((....)))))))..((((((....))))))......))))))).))))))......))))........ (-18.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAUAAAAGGUUAUUUGAUUCAAUUCAUCAUUUUUUUGCCGAAAUAUUUUUCGAAUCAAAGAAUUAUUCCUUUAUUUGUGCUUAAAAUUAGCAUGUAAUAAUAAUUAAAAUAUCAAACC ........((((.((((((((.......((......))..((((....)))))))))))).(((((((...((((..(((((.......))))))))).))))))).........)))) (-15.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 212 95 57 160 121 tfor:TF2575|5end_methionine_aminopeptidase_2745126..2745356_POSITIVE 206 105 66 91 57 tfor:TF1251|5end_riboflavin_synthase,_alpha_subunit_1333744..1333974_POSITIVE 199 100 59 90 46 tfor:TF2320|5end_hypothetical_protein_2476654..2476884_POSITIVE 192 69 21 99 44 tfor:TF2469|5end_oxidoreductase,_possible_Gfo/Idh/MocA_family_2646505..2646735_POSITIVE 186 82 61 162 141 tfor:TF0036|5end_beta-hexosaminidase_precursor_35821..36051_POSITIVE 184 81 48 188 162 tfor:TF2709|5end_hypothetical_protein_2882160..2882390_POSITIVE 183 110 77 89 56 tfor:TF2127|5end_hypothetical_protein_2304038..2304268_POSITIVE 177 84 55 221 181 tfor:TF2009|5end_octaprenyl-diphosphate_synthase_ispB_2162131..2162361_POSITIVE 177 71 39 154 118 tfor:TF1695|5end_excisionase_1816522..1816752_POSITIVE 174 100 59 108 67 tfor:TF1326|5end_hypothetical_protein_1403844..1404074_POSITIVE 174 90 50 97 55 tfor:TF0221|5end_aminoacyl-histidine_dipeptidase_247782..248012_POSITIVE 173 69 27 193 145 tfor:TF0061|5end_hypothetical_protein_62833..63063_POSITIVE 171 99 66 213 179 tfor:TF1976|5end_RNA_polymerase_ECF-type_sigma_factor_2122520..2122750_POSITIVE 170 79 49 147 119 tfor:TF0758|5end_peptidyl-tRNA_hydrolase_805307..805537_POSITIVE 170 82 49 108 82 tfor:TF0407|5end_lipoate-protein_ligase_B_(lipoyltransferase)_425084..425314_POSITIVE 170 94 57 228 197 tfor:TF0082|5end_transcriptional_regulator,_TetR_family_88516..88746_POSITIVE 168 82 50 91 53 tfor:TF1030|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_1088229..1088459_POSITIVE 167 84 56 43 8 tfor:TF2576|5end_translation_initiation_factor_IF-1_2745912..2746142_POSITIVE 167 94 64 197 168 tfor:TF1670|5end_ATP_synthase,_alpha_subunit_1784455..1784685_POSITIVE 167 93 56 94 64 tfor:TF0088|5end_ABC_transporter,_permease_component_94299..94529_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 206 105 66 68 34 tfor:TF1250|3end_possible_cation_efflux_pump_1333801..1333951_POSITIVE 192 69 21 96 41 tfor:TF2468|3end_hypothetical_protein_2646582..2646732_POSITIVE 184 81 48 147 121 tfor:TF2708|3end_DNA_gyrase_subunit_B_2882199..2882349_POSITIVE 182 85 57 142 115 tfor:TF2574|3end_preprotein_translocase_SecY_2745200..2745350_POSITIVE 174 100 59 130 89 tfor:TF1324|3end_conserved_hypothetical_protein_1403946..1404096_POSITIVE 174 90 50 44 2 tfor:TF0220|3end_conserved_hypothetical_protein_247809..247959_POSITIVE 173 69 27 143 95 tfor:TF0060|3end_conserved_hypothetical_protein_62863..63013_POSITIVE 170 79 49 53 25 tfor:TF0757|3end_50S_ribosomal_protein_L25;_general_stress_protein_805293..805443_POSITIVE 170 82 49 108 82 tfor:TF0406|3end_monofunctional_biosynthetic_peptidoglycan_transglycosylase_425164..425314_POSITIVE 170 94 57 126 95 tfor:TF0081|3end_pantothenate_kinase_88494..88644_POSITIVE 168 82 50 50 12 tfor:TF1029|3end_conserved_hypothetical_protein;_possible_phytoene_dehydrogenase_1088268..1088418_POSITIVE 167 84 56 36 1 tfor:TF2575|3end_methionine_aminopeptidase_2745985..2746135_POSITIVE 167 93 56 83 53 tfor:TF0087|3end_ABC_transporter,_permease_component_94368..94518_POSITIVE 163 83 55 119 93 tfor:TF0767|3end_conserved_hypothetical_protein_813820..813970_POSITIVE 163 76 33 82 35 tfor:TF0734|3end_hypothetical_protein_787064..787214_POSITIVE 162 89 67 140 116 tfor:TF0050|3end_hypothetical_protein_55300..55450_POSITIVE 160 81 47 101 60 tfor:TF0113|3end_4-deoxy-L-threo-5-hexosulose-uronate_ketol-isomerase_124068..124218_POSITIVE 159 105 61 61 14 tfor:TF0244|3end_DNA_recombination_protein_275776..275926_POSITIVE 158 82 48 111 59 tfor:TF3111|3end_hypothetical_protein_3346836..3346986_POSITIVE 158 84 60 146 121 tfor:TF2433|3end_possible_UDP-2,3-diacylglucosamine_hydrolase_2614043..2614193_POSITIVE