Origin IGS: taaactctacttcgggaggcagaaagcccaagagatattgatgttgggtagcccccccgaggacgagtccttccgcccccccccccttttttctttagattgagaaaagggggctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagcccccttttctcaatctaaagaaaaaagggggggggggcggaaggactcgtcctcgggggggctacccaacatcaatatctcttgggctttctgcctcccgaagtagagttta Mask Tandem Repeat Region ================================================ taaactctacttcgggaggcagaaagcccaagagatattgatgttgggtagcccccccgaggacgagtccttccgcccccccccccttttttctttagattgagaaaagggggctta Find is-nt database================================================ Query_seq: TF1014:TF1015|TF1014:TF1015:glucose-6-phosphate isomerase:conserved hypothetical protein:->->:1072535..1072651 117 taaactctacttcgggaggcagaaagcccaagagatattgatgttgggtagcccccccgaggacgagtccttccgcccccccccccttttttctttagattgagaaaagggggctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF1014:TF1015|TF1014:TF1015:glucose-6-phosphate isomerase:conserved hypothetical protein:->->:1072535..1072651 117 taaactctacttcgggaggcagaaagcccaagagatattgatgttgggtagcccccccgaggacgagtccttccgcccccccccccttttttctttagattgagaaaagggggctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF1014:TF1015|TF1014:TF1015:glucose-6-phosphate isomerase:conserved hypothetical protein:->->:1072535..1072651 117 taaactctacttcgggaggcagaaagcccaagagatattgatgttgggtagcccccccgaggacgagtccttccgcccccccccccttttttctttagattgagaaaagggggctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaactctacttcgggaggcagaaagcccaagagatattgatgttgggtagcccccccgaggacgagtccttccgcccccccccccttttttctttagattgagaaaagggggctta Predict ORF larger than 30AA ================================================ taaactctacttcgggaggcagaaagcccaagagatattgatgttgggtagcccccccgaggacgagtccttccgcccccccccccttttttctttagattgagaaaagggggctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaactctacttcgggaggcagaaagcccaagagatattgatgttgggtagcccccccgaggacgagtccttccgcccccccccccttttttctttagattgagaaaagggggctta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF1014:TF1015|TF1014:TF1015:glucose-6-phosphate isomerase:conserved hypothetical protein:->->:1072535..1072651 117 taaactctacttcgggaggcagaaagcccaagagatattgatgttgggtagcccccccgaggacgagtccttccgcccccccccccttttttctttagattgagaaaagggggctta Intra-Species Hit: Count: 1 Min: 1 Max: 117 Len: 117 Subject: tfor_TF1014_TF1015|glucose-6-phosphate isomerase:conserved hypothetical protein|POSITIVE:POSITIVE|[1072535,1072651]|117 HSP 1 e-value: 1.0E-40 bit: 167.0 Len: 117 Query Start:1 Query End:117 Subject Strand: POSITIVE Subject Start: 1 Subject End: 117 taaactctacttcgggaggcagaaagcccaagagatattgatgttgggtagcccccccgaggacgagtccttccgnnnnnnnnnnnttttttctttagattgagaaaagggggctta taaactctacttcgggaggcagaaagcccaagagatattgatgttgggtagcccccccgaggacgagtccttccgcccccccccccttttttctttagattgagaaaagggggctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACUCUACUUCGGGAGGCAGAAAGCCCAAGAGAUAUUGAUGUUGGGUAGCCCCCCCGAGGACGAGUCCUUCCGCCCCCCCCCCCUUUUUUCUUUAGAUUGAGAAAAGGGGGCUUA ...((((..(((((((.(((.....((((((.(........).)))))).)))..)))))))..)))).............((((((((((((........)))))))))))).... (-46.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGCCCCCUUUUCUCAAUCUAAAGAAAAAAGGGGGGGGGGGCGGAAGGACUCGUCCUCGGGGGGGCUACCCAACAUCAAUAUCUCUUGGGCUUUCUGCCUCCCGAAGUAGAGUUUA ....(((((((((.((........)).)))))))))(((((((((((((....(((((....)))))..(((((............))))))))))))))))))............. (-50.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 454 81 41 199 150 tfor:TF0685|5end_hypothetical_protein_730852..731082_POSITIVE 447 88 46 231 191 tfor:TF0830|5end_hypothetical_protein_887610..887840_POSITIVE 431 100 52 98 35 tfor:TF2120|5end_holliday_junction_endonuclease_2292108..2292338_POSITIVE 426 78 44 74 40 tfor:TF0816|5end_hypothetical_protein_874217..874447_POSITIVE 418 90 41 145 94 tfor:TF0662|5end_hypothetical_protein_708316..708546_POSITIVE 412 106 61 153 111 tfor:TF1470|5end_phosphohydrolase,_Icc_family_1563144..1563374_POSITIVE 410 79 41 133 88 tfor:TF2218|5end_two-component_system_response_regulator_2390162..2390392_POSITIVE 404 89 42 84 29 tfor:TF3107|5end_hypothetical_protein_3338459..3338689_POSITIVE 399 87 51 201 158 tfor:TF1767|5end_3-dehydroquinate_synthase_1905492..1905722_POSITIVE 392 80 31 208 145 tfor:TF1416|5end_outer_membrane_protein_1497576..1497806_POSITIVE 390 89 51 166 110 tfor:TF2818|5end_acetylornithine_aminotransferase_3007739..3007969_POSITIVE 370 89 45 178 139 tfor:TF1840|5end_transposase_1990221..1990451_POSITIVE 368 78 41 172 129 tfor:TF2478|5end_hypothetical_protein_2655375..2655605_POSITIVE 365 76 31 61 2 tfor:TF3111|5end_hypothetical_protein_3346601..3346831_POSITIVE 360 80 33 65 1 tfor:TF2453|5end_glutamate_racemase_2630422..2630652_POSITIVE 359 85 43 192 126 tfor:TF0955|5end_conserved_hypothetical_protein_999215..999445_POSITIVE 358 59 11 206 165 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 358 59 11 206 165 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 355 95 52 119 64 tfor:TF1137|5end_asparaginyl-tRNA_synthetase_(asparagine--tRNA_ligase)_1212443..1212673_POSITIVE 353 87 41 146 92 tfor:TF2904|5end_ATP-dependent__helicase,_possible_UvrD/REP_helicase_domain_protein_3098903..3099133_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 456 89 46 50 9 tfor:TF0828|3end_hypothetical_protein_887508..887658_POSITIVE 450 79 38 48 1 tfor:TF1599|3end_ribonuclease_P_protein_component_1714054..1714204_POSITIVE 431 100 52 98 35 tfor:TF2119|3end_conserved_hypothetical_protein_2292188..2292338_POSITIVE 418 90 44 78 19 tfor:TF1838|3end_tRNA/rRNA_methyltransferase_1988745..1988895_POSITIVE 410 79 41 56 11 tfor:TF2217|3end_hypothetical_protein_2390165..2390315_POSITIVE 386 87 42 151 98 tfor:TF3106|3end_hypothetical_protein_3338608..3338758_POSITIVE 374 80 32 85 42 tfor:TF2556|3end_conserved_hypothetical_protein_2737095..2737245_POSITIVE 367 83 41 144 90 tfor:TF1443|3end_hypothetical_protein_1528203..1528353_POSITIVE 364 89 46 73 37 tfor:TF0821|3end_hypothetical_protein_880195..880345_POSITIVE 364 89 46 92 56 tfor:TF0370|3end_hypothetical_protein_386317..386467_POSITIVE 353 87 41 149 95 tfor:TF2903|3end_translation_initiation_factor_SUI1_3098986..3099136_POSITIVE 348 89 41 66 2 tfor:TF2651|3end_hypothetical_protein_2822014..2822164_POSITIVE 341 89 46 151 115 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 337 87 51 45 2 tfor:TF2999|3end_conserved_hypothetical_protein_3215891..3216041_POSITIVE 337 88 46 61 1 tfor:TF1588|3end_hypothetical_protein_1702560..1702710_POSITIVE 336 88 43 137 92 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 335 84 59 27 3 tfor:TF1169|3end_lipoprotein_ABC_transporter,_permease_component_1251573..1251723_POSITIVE 329 85 42 79 37 tfor:TF2366|3end_conserved_hypothetical_protein_2531892..2532042_POSITIVE 327 79 31 87 26 tfor:TF2604|3end_possible_membrane_transport_protein_2779832..2779982_POSITIVE 327 79 44 58 7 tfor:TF2322|3end_conserved_hypothetical_protein;_possible_lipoprotein_2483785..2483935_POSITIVE