Origin IGS: aacaaattcttctcttttatttttagatgctgcaaaagtaataaatttagtaaaaaggatcgtcgaatcattttcaaattgtatctttgccttccggacaaaacgaattattaattttatttatttacat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atgtaaataaataaaattaataattcgttttgtccggaaggcaaagatacaatttgaaaatgattcgacgatcctttttactaaatttattacttttgcagcatctaaaaataaaagagaagaatttgtt Mask Tandem Repeat Region ================================================ aacaaattcttctcttttatttttagatgctgcaaaagtaataaatttagtaaaaaggatcgtcgaatcattttcaaattgtatctttgccttccggacaaaacgaattattaattttatttatttacat Find is-nt database================================================ Query_seq: TF0998:TF0999|TF0998:TF0999:[acyl-carrier-protein] S-malonyltransferase:hypothetical protein:->->:1053038..1053167 130 aacaaattcttctcttttatttttagatgctgcaaaagtaataaatttagtaaaaaggatcgtcgaatcattttcaaattgtatctttgccttccggacaaaacgaattattaattttatttatttacat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0998:TF0999|TF0998:TF0999:[acyl-carrier-protein] S-malonyltransferase:hypothetical protein:->->:1053038..1053167 130 aacaaattcttctcttttatttttagatgctgcaaaagtaataaatttagtaaaaaggatcgtcgaatcattttcaaattgtatctttgccttccggacaaaacgaattattaattttatttatttacat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0998:TF0999|TF0998:TF0999:[acyl-carrier-protein] S-malonyltransferase:hypothetical protein:->->:1053038..1053167 130 aacaaattcttctcttttatttttagatgctgcaaaagtaataaatttagtaaaaaggatcgtcgaatcattttcaaattgtatctttgccttccggacaaaacgaattattaattttatttatttacat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aacaaattcttctcttttatttttagatgctgcaaaagtaataaatttagtaaaaaggatcgtcgaatcattttcaaattgtatctttgccttccggacaaaacgaattattaattttatttatttacat Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 19 End: 111 .................. M F R C C K S N K F S K K D R R I I F K L Y L C L P D K T N Y ................... Protein_Len: 30 Strand: + Start: 41 End: 130 ........................................ M N L V K R I V E S F S N C I F A F R T K R I I N F I Y L H Protein_Len: 33 Strand: - Start: 3 End: 101 .. L N K E K I K L H Q L L L L N L L F S R R I M K L N Y R Q R G S M ............................. Protein_Len: 40 Strand: - Start: 1 End: 120 V F E E R K N K S A A F T I F K T F L I T S D N E F Q I K A K R V F R I I L K M .......... aacaaattcttctcttttatttttagatgctgcaaaagtaataaatttagtaaaaaggatcgtcgaatcattttcaaattgtatctttgccttccggacaaaacgaattattaattttatttatttacat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aacaaattcttctcttttatttttagatgctgcaaaagtaataaatttagtaaaaaggatcgtcgaatcattttcaaattgtatctttgccttccggacaaaacgaattattaattttatttatttacat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0998:TF0999|TF0998:TF0999:[acyl-carrier-protein] S-malonyltransferase:hypothetical protein:->->:1053038..1053167 130 aacaaattcttctcttttatttttagatgctgcaaaagtaataaatttagtaaaaaggatcgtcgaatcattttcaaattgtatctttgccttccggacaaaacgaattattaattttatttatttacat Intra-Species Hit: Count: 1 Min: 1 Max: 130 Len: 130 Subject: tfor_TF0998_TF0999|[acyl-carrier-protein] S-malonyltransferase:hypothetical protein|POSITIVE:POSITIVE|[1053038,1053167]|130 HSP 1 e-value: 4.0E-68 bit: 258.0 Len: 130 Query Start:1 Query End:130 Subject Strand: POSITIVE Subject Start: 1 Subject End: 130 aacaaattcttctcttttatttttagatgctgcaaaagtaataaatttagtaaaaaggatcgtcgaatcattttcaaattgtatctttgccttccggacaaaacgaattattaattttatttatttacat aacaaattcttctcttttatttttagatgctgcaaaagtaataaatttagtaaaaaggatcgtcgaatcattttcaaattgtatctttgccttccggacaaaacgaattattaattttatttatttacat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACAAAUUCUUCUCUUUUAUUUUUAGAUGCUGCAAAAGUAAUAAAUUUAGUAAAAAGGAUCGUCGAAUCAUUUUCAAAUUGUAUCUUUGCCUUCCGGACAAAACGAAUUAUUAAUUUUAUUUAUUUACAU ....(((((.((..(((((((((((..((((.....)))).))))...)))))))..)).....(((.....)))..........((((((....)).))))..)))))..................... (-13.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUGUAAAUAAAUAAAAUUAAUAAUUCGUUUUGUCCGGAAGGCAAAGAUACAAUUUGAAAAUGAUUCGACGAUCCUUUUUACUAAAUUUAUUACUUUUGCAGCAUCUAAAAAUAAAAGAGAAGAAUUUGUU .((((((..((((((.(((........(((((.((....)))))))........((((((.((((....)))).)))))).))).))))))...)))))).............................. (-17.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 323 100 53 119 80 tfor:TF2456|5end_ABC_transporter,_ATP-binding_protein_2632376..2632606_POSITIVE 291 98 55 52 8 tfor:TF1024|5end_50S_ribosomal_protein_L19_1081948..1082178_POSITIVE 275 98 61 88 46 tfor:TF1548|5end_thiophene_and_furan_oxidation_protein_1652501..1652731_POSITIVE 270 98 55 207 159 tfor:TF0786|5end_pyridoxal_phosphate_biosynthetic_protein_838563..838793_POSITIVE 264 106 61 178 129 tfor:TF2181|5end_conserved_hypothetical_protein_2353870..2354100_POSITIVE 262 100 56 86 44 tfor:TF0265|5end_conserved_hypothetical_protein,_Rhs_family_293279..293509_POSITIVE 258 107 68 212 166 tfor:TF2523|5end_NAD(P)H-flavin_oxidoreductase_2702114..2702344_POSITIVE 251 98 57 84 37 tfor:TF0775|5end_ABC_transporter,_permease_component_823664..823894_POSITIVE 250 34 4 90 65 tfor:TF1075|5end_conserved_hypothetical_protein;_possible_lantibiotic_biosynthesis_protein_1144428..1144658_POSITIVE 249 100 56 126 80 tfor:TF2568|5end_30S_ribosomal_protein_S8_2741207..2741437_POSITIVE 247 106 61 69 19 tfor:TF1760|5end_guanosine-3',5'-bis(diphosphate)_3'-pyrophosphohydrolase_1897081..1897311_POSITIVE 245 100 57 228 187 tfor:TF1580|5end_conserved_hypothetical_protein;_possible_fibronectin_domain_containing_protein_1687187..1687417_POSITIVE 241 99 58 220 182 tfor:TF2561|5end_50S_ribosomal_protein_L16_2738693..2738923_POSITIVE 241 106 76 168 135 tfor:TF2008|5end_DNA_polymerase_I_2159302..2159532_POSITIVE 238 98 54 51 6 tfor:TF1621|5end_DNA_replication_and_repair_protein_RecF_1735293..1735523_POSITIVE 238 98 55 148 108 tfor:TF0236|5end_multidrug_resistance_protein_261779..262009_POSITIVE 234 107 62 61 8 tfor:TF2395|5end_conserved_hypothetical_protein_2566532..2566762_POSITIVE 234 99 57 227 188 tfor:TF2200|5end_outer_membrane_protein,_TonB_dependent_receptor_2376495..2376725_POSITIVE 234 97 54 98 57 tfor:TF0336|5end_Xaa-Pro_aminopeptidase_360613..360843_POSITIVE 233 97 57 227 182 tfor:TF2482|5end_hypothetical_protein_2658503..2658733_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 323 100 53 100 61 tfor:TF2455|3end_conserved_hypothetical_protein;_possible_membrane_protein_2632437..2632587_POSITIVE 280 105 61 131 75 tfor:TF0294|3end_possible_translation_factor_321902..322052_POSITIVE 273 100 56 128 77 tfor:TF2166|3end_hypothetical_protein_2342362..2342512_POSITIVE 263 100 56 128 77 tfor:TF0366|3end_hypothetical_protein_383811..383961_POSITIVE 263 100 56 128 77 tfor:TF0362|3end_hypothetical_protein_380199..380349_POSITIVE 261 94 63 75 36 tfor:TF0793|3end_conserved_hypothetical_protein;_possible_AAA_superfamily_ATPase_851291..851441_POSITIVE 258 107 68 147 101 tfor:TF2522|3end_deoxyuridine_5'-triphosphate_nucleotidohydrolase_(dUTPase)_2702129..2702279_POSITIVE 256 106 78 120 90 tfor:TF2395|3end_conserved_hypothetical_protein_2567664..2567814_POSITIVE 254 96 58 91 50 tfor:TF1861|3end_conserved_hypothetical_protein;_possible_membrane_protein_2018058..2018208_POSITIVE 252 97 56 131 85 tfor:TF0123|3end_conserved_hypothetical_protein_134947..135097_POSITIVE 251 98 57 70 23 tfor:TF0774|3end_membrane-fusion_protein;_36_kDa_antigen_823730..823880_POSITIVE 250 34 4 99 74 tfor:TF1074|3end_hypothetical_protein_1144517..1144667_POSITIVE 249 100 56 52 6 tfor:TF2567|3end_30S_ribosomal_protein_S14_2741213..2741363_POSITIVE 247 106 61 52 2 tfor:TF1759|3end_membrane-bound_lytic_murein_transglycosylase_D_precursor_1897144..1897294_POSITIVE 241 106 76 113 80 tfor:TF2007|3end_uracil_phosphoribosyltransferase_2159327..2159477_POSITIVE 238 98 55 138 98 tfor:TF0235|3end_hypothetical_protein_261849..261999_POSITIVE 234 97 54 116 75 tfor:TF0337|3end_hypothetical_protein_360711..360861_POSITIVE 231 103 62 51 15 tfor:TF0422|3end_proline_dehydrogenase_438695..438845_POSITIVE 229 106 82 102 81 tfor:TF1324|3end_conserved_hypothetical_protein_1403946..1404096_POSITIVE 228 100 67 36 7 tfor:TF2985|3end_glycosyltransferase_protein_3201646..3201796_POSITIVE