Origin IGS: taaagaatgctgtgttgtcgtcaagctatgccctgtcaaccgtatcgaacaacgcattcaggaggcagggaagctcggattcacacgcatgatcgttccgcagatgaaagggctggaagcgttggaaagcaagatgaaaatctttcaggtcagaaaagtagaagaagccttccggcagctattcggataaggatgattttaacaggcaatacagatagcggaagttttttgatctactgctgcaaga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tcttgcagcagtagatcaaaaaacttccgctatctgtattgcctgttaaaatcatccttatccgaatagctgccggaaggcttcttctacttttctgacctgaaagattttcatcttgctttccaacgcttccagccctttcatctgcggaacgatcatgcgtgtgaatccgagcttccctgcctcctgaatgcgttgttcgatacggttgacagggcatagcttgacgacaacacagcattcttta Mask Tandem Repeat Region ================================================ taaagaatgctgtgttgtcgtcaagctatgccctgtcaaccgtatcgaacaacgcattcaggaggcagggaagctcggattcacacgcatgatcgttccgcagatgaaagggctggaagcgttggaaagcaagatgaaaatctttcaggtcagaaaagtagaagaagccttccggcagctattcggataaggatgattttaacaggcaatacagatagcggaagttttttgatctactgctgcaaga Find is-nt database================================================ Query_seq: TF0989:TF0990|TF0989:TF0990:conserved hypothetical protein:hypothetical protein:->->:1045288..1045534 247 taaagaatgctgtgttgtcgtcaagctatgccctgtcaaccgtatcgaacaacgcattcaggaggcagggaagctcggattcacacgcatgatcgttccgcagatgaaagggctggaagcgttggaaagcaagatgaaaatctttcaggtcagaaaagtagaagaagccttccggcagctattcggataaggatgattttaacaggcaatacagatagcggaagttttttgatctactgctgcaaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0989:TF0990|TF0989:TF0990:conserved hypothetical protein:hypothetical protein:->->:1045288..1045534 247 taaagaatgctgtgttgtcgtcaagctatgccctgtcaaccgtatcgaacaacgcattcaggaggcagggaagctcggattcacacgcatgatcgttccgcagatgaaagggctggaagcgttggaaagcaagatgaaaatctttcaggtcagaaaagtagaagaagccttccggcagctattcggataaggatgattttaacaggcaatacagatagcggaagttttttgatctactgctgcaaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0989:TF0990|TF0989:TF0990:conserved hypothetical protein:hypothetical protein:->->:1045288..1045534 247 taaagaatgctgtgttgtcgtcaagctatgccctgtcaaccgtatcgaacaacgcattcaggaggcagggaagctcggattcacacgcatgatcgttccgcagatgaaagggctggaagcgttggaaagcaagatgaaaatctttcaggtcagaaaagtagaagaagccttccggcagctattcggataaggatgattttaacaggcaatacagatagcggaagttttttgatctactgctgcaaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 2 Min: 32 Max: 187 Len: 156 Subject: UniRef90_Q64YS2 Cluster: Putative DNA repair protein; n=3; Bacteroides|Rep: Putative DNA repair protein - Bacteroides fragilis HSP 1 e-value: 5.0E-9 bit: 62.4 Len: 156 Query Start:32 Query End:187 Subject Strand: null Subject Start: 402 Subject End: 455 ............................... P V N R I E Q R I Q E A G K L G F T R M I V P Q - - M K G L E A L E S K M K I F Q V R K V E E A F R Q L F G ............................................................ ............................... P V N R I E Q R I G E A E K L G F K R F I L P K Y N M Q G I D T K K L R I E L V P V R K V E E A F R T L F G ............................................................ Subject: UniRef90_A3J3P0 Cluster: DNA repair protein; n=1; Flavobacteria bacterium BAL38|Rep: DNA repair protein - Flavobacteria bacterium BAL38 HSP 1 e-value: 8.0E-6 bit: 51.6 Len: 156 Query Start:32 Query End:187 Subject Strand: null Subject Start: 402 Subject End: 453 ............................... P V N R I E Q R I Q E A G K L G F T R M I V P Q M K G L E A L E S K M K I F Q V R K V E E A F R Q L F G ............................................................ ............................... P V N R I E Q R I Q E A E K L G F T T I F V S K H N K I S L K N T G I K I V L V A K I E D V V S E L F G ............................................................ taaagaatgctgtgttgtcgtcaagctatgcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaaggatgattttaacaggcaatacagatagcggaagttttttgatctactgctgcaaga Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: + Start: 44 End: 187 ........................................... M E Q R I Q E A G K L G F T R M I V P Q M K G L E A L E S K M K I F Q V R K V E E A F R Q L F G ............................................................ Protein_Len: 60 Strand: + Start: 7 End: 246 ...... M L C C R Q A M P C Q P Y R T T H S G G R E A R I H T H D R S A D E R A G S V G K Q D E N L S G Q K S R R S L P A A I R . Protein_Len: 50 Strand: - Start: 29 End: 178 ............................ A R D V T D F L A N L L C P L E S E C A H D N R L H F P Q F R Q F A L H F D K L D S F Y F F G E P M ..................................................................... taaagaatgctgtgttgtcgtcaagctatgcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaaggatgattttaacaggcaatacagatagcggaagttttttgatctactgctgcaaga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaagaatgctgtgttgtcgtcaagctatgcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaaggatgattttaacaggcaatacagatagcggaagttttttgatctactgctgcaaga Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0989:TF0990|TF0989:TF0990:conserved hypothetical protein:hypothetical protein:->->:1045288..1045534 247 taaagaatgctgtgttgtcgtcaagctatgcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaaggatgattttaacaggcaatacagatagcggaagttttttgatctactgctgcaaga Intra-Species Hit: Count: 1 Min: 1 Max: 247 Len: 247 Subject: tfor_TF0989_TF0990|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1045288,1045534]|247 HSP 1 e-value: 5.0E-26 bit: 119.0 Len: 60 Query Start:188 Query End:247 Subject Strand: POSITIVE Subject Start: 188 Subject End: 247 ...........................................................................................................................................................................................taaggatgattttaacaggcaatacagatagcggaagttttttgatctactgctgcaaga ...........................................................................................................................................................................................taaggatgattttaacaggcaatacagatagcggaagttttttgatctactgctgcaaga HSP 2 e-value: 9.0E-9 bit: 61.9 Len: 31 Query Start:1 Query End:31 Subject Strand: POSITIVE Subject Start: 1 Subject End: 31 taaagaatgctgtgttgtcgtcaagctatgc........................................................................................................................................................................................................................ taaagaatgctgtgttgtcgtcaagctatgc........................................................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAGAAUGCUGUGUUGUCGUCAAGCUAUGCCCUGUCAACCGUAUCGAACAACGCAUUCAGGAGGCAGGGAAGCUCGGAUUCACACGCAUGAUCGUUCCGCAGAUGAAAGGGCUGGAAGCGUUGGAAAGCAAGAUGAAAAUCUUUCAGGUCAGAAAAGUAGAAGAAGCCUUCCGGCAGCUAUUCGGAUAAGGAUGAUUUUAACAGGCAAUACAGAUAGCGGAAGUUUUUUGAUCUACUGCUGCAAGA ......((((((((...(((...((((...(((((((..((..((((.....)).))...)).))))))).)))))))...)))).)))).........((((......((.(((((...(((....))).((((....))))))))).)).....((((((....(((((((((.......)))))................))))....((((.(((....))).)))).)))))).)))).... (-67.69) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUUGCAGCAGUAGAUCAAAAAACUUCCGCUAUCUGUAUUGCCUGUUAAAAUCAUCCUUAUCCGAAUAGCUGCCGGAAGGCUUCUUCUACUUUUCUGACCUGAAAGAUUUUCAUCUUGCUUUCCAACGCUUCCAGCCCUUUCAUCUGCGGAACGAUCAUGCGUGUGAAUCCGAGCUUCCCUGCCUCCUGAAUGCGUUGUUCGAUACGGUUGACAGGGCAUAGCUUGACGACAACACAGCAUUCUUUA .....(((.((((((.............(((((.((.((.(..((.......))..)..)).)).))))).(((....)))....))))))...))).....(((((...................((.((((.((..........))))))))...((((((((.....((((((.(((((.(..(((.((.((.....)))).)))..).)))))...))))))......)))).))))))))). (-53.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 520 120 73 168 117 tfor:TF3105|5end_hypothetical_protein_3338068..3338298_POSITIVE 436 120 75 206 163 tfor:TF1655|5end_long-chain-fatty-acid-CoA_ligase_1769279..1769509_POSITIVE 435 120 75 182 132 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 435 120 75 182 132 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 435 120 75 220 170 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 435 120 75 168 118 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 428 120 75 213 163 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 415 77 32 211 154 tfor:TF1440|5end_hypothetical_protein_1524599..1524829_POSITIVE 396 98 52 151 90 tfor:TF0925|5end_hypothetical_protein_961736..961966_POSITIVE 389 100 53 107 54 tfor:TF0851|5end_helicase_SNF2_family_899486..899716_POSITIVE 384 100 53 51 2 tfor:TF2621|5end_conserved_hypothetical_protein;_possible_SAM-dependent_methyltransferase_2796760..2796990_POSITIVE 382 100 53 190 120 tfor:TF1500|5end_conserved_hypothetical_protein_1599693..1599923_POSITIVE 370 100 52 212 162 tfor:TF2248|5end_transfer_region-related_protein,_TraB_2422551..2422781_POSITIVE 365 76 32 172 117 tfor:TF1559|5end_glycosyltransferase_1665879..1666109_POSITIVE 365 100 53 209 144 tfor:TF1194|5end_glycogen_debranching_enzyme_1276627..1276857_POSITIVE 357 77 31 49 10 tfor:TF2014|5end_dipeptidyl_aminopeptidase_IV_2169398..2169628_POSITIVE 356 48 4 185 135 tfor:TF2781|5end_hypothetical_protein_2965822..2966052_POSITIVE 356 79 31 82 36 tfor:TF2080|5end_carboxyl-terminal_protease_2246880..2247110_POSITIVE 354 100 53 155 108 tfor:TF2229.1|5end_rteR_regulatory_ortholog_(with_internal_stop)_2399454..2399684_POSITIVE 351 99 56 61 17 tfor:TF2097|5end_glucose_inhibited_division_protein_A_2269137..2269367_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 351 88 44 75 22 tfor:TF0795|3end_hypothetical_protein_853199..853349_POSITIVE 347 100 54 62 24 tfor:TF2332|3end_signal_peptidase_I_2492613..2492763_POSITIVE 347 106 64 67 4 tfor:TF2007|3end_uracil_phosphoribosyltransferase_2159327..2159477_POSITIVE 345 100 54 48 1 tfor:TF3153|3end_conserved_hypothetical_protein_3389324..3389474_POSITIVE 345 100 53 71 8 tfor:TF1944|3end_conserved_hypothetical_protein_2093309..2093459_POSITIVE 344 47 5 136 89 tfor:TF0479|3end_transcriptional_regulator_498405..498555_POSITIVE 340 75 33 148 106 tfor:TF2453|3end_glutamate_racemase_2631326..2631476_POSITIVE 339 117 73 134 96 tfor:TF3102|3end_hypothetical_protein_3332969..3333119_POSITIVE 331 130 81 131 88 tfor:TF0462|3end_possible_cobalamin_biosynthesis_precorrin-3_methylase_479095..479245_POSITIVE 331 74 35 144 113 tfor:TF0251|3end_conserved_hypothetical_protein;_possible_exporter_283529..283679_POSITIVE 330 106 61 148 102 tfor:TF1918|3end_hypothetical_protein_2070105..2070255_POSITIVE 330 100 51 133 81 tfor:TF1583|3end_conserved_hypothetical_protein;_possible_endonuclease_1690223..1690373_POSITIVE 328 126 85 66 17 tfor:TF1480|3end_ABC_transporter,_permease_component_1574866..1575016_POSITIVE 327 130 83 103 50 tfor:TF1233|3end_dihydrodipicolinate_synthase_1318855..1319005_POSITIVE 325 55 11 43 1 tfor:TF1737|3end_outer_membrane_protein,_TonB_dependent_receptor_1865306..1865456_POSITIVE 323 130 85 76 17 tfor:TF2749|3end_possible_hemolysin_2934518..2934668_POSITIVE 323 54 11 70 15 tfor:TF0818|3end_bifunctional_protein:__aspartokinase_I;_homoserine_dehydrogenase_878517..878667_POSITIVE 322 99 61 137 101 tfor:TF2255|3end_transfer_region-related_protein,_TraH_2427961..2428111_POSITIVE 319 80 31 111 52 tfor:TF1301|3end_hypothetical_protein_1383487..1383637_POSITIVE 318 60 11 145 96 tfor:TF2370|3end_tRNA_delta-2_isopentenylpyrophosphate_transferase_2537044..2537194_POSITIVE