Origin IGS: taaattgatggagtttgctttacaaagtacatttgatactcactcataacagatttgttctaatctccttgttcttacatctcgtatatcctgcatcttatacgttttttccgtcggggaagtctttttcgtttcagtggcgtaaaaaaaggagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtcgtaacggcttgccgctccgaccttttttgtccgtgaaagcccaaaactacctttgtcactgacaacgaacaaccgagcccgacatgatatacgggcataagtggaggatgtgcagggagagaagcaggacaaaacaaaaaacacagtctttaaggactcattttatcataagaatagaatacacaaaaacaaaaagctgccccaatgacggcttaaggaaaaaatcaagagagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgtaaagcgttgtatatcattacggaagataggtcgcccaatcattacctatagcattttataactcattaggcattgaataccaatcatttgaaccaaaatgatagattttacggagacagtttttctctatccatattttgtgtcaacccatataatacaagaactgcgatttatcgggtaagccaataatcatctttgttttttaggttcccacctatttatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taataaataggtgggaacctaaaaaacaaagatgattattggcttacccgataaatcgcagttcttgtattatatgggttgacacaaaatatggatagagaaaaactgtctccgtaaaatctatcattttggttcaaatgattggtattcaatgcctaatgagttataaaatgctataggtaatgattgggcgacctatcttccgtaatgatatacaacgctttacataactcgaataacctgttgcaaagataaaggtatatcgtgagaaaaaagaattttctctcttgattttttccttaagccgtcattggggcagctttttgtttttgtgtattctattcttatgataaaatgagtccttaaagactgtgttttttgttttgtcctgcttctctccctgcacatcctccacttatgcccgtatatcatgtcgggctcggttgttcgttgtcagtgacaaaggtagttttgggctttcacggacaaaaaaggtcggagcggcaagccgttacgacgacaatctccacacttccatttcattgcaggtagtattctcgccgtaaaaccttgtttgtcctaagccctcctttttttacgccactgaaacgaaaaagacttccccgacggaaaaaacgtataagatgcaggatatacgagatgtaagaacaaggagattagaacaaatctgttatgagtgagtatcaaatgtactttgtaaagcaaactccatcaattta Mask Tandem Repeat Region ================================================ taaattgatggagtttgctttacaaagtacatttgatactcactcataacagatttgttctaatctccttgttcttacatctcgtatatcctgcatcttatacgttttttccgtcggggaagtctttttcgtttcagtggcgtaaaaaaaggagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtcgtaacggcttgccgctccgaccttttttgtccgtgaaagcccaaaactacctttgtcactgacaacgaacaaccgagcccgacatgatatacgggcataagtggaggatgtgcagggagagaagcaggacaaaacaaaaaacacagtctttaaggactcattttatcataagaatagaatacacaaaaacaaaaagctgccccaatgacggcttaaggaaaaaatcaagagagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgtaaagcgttgtatatcattacggaagataggtcgcccaatcattacctatagcattttataactcattaggcattgaataccaatcatttgaaccaaaatgatagattttacggagacagtttttctctatccatattttgtgtcaacccatataatacaagaactgcgatttatcgggtaagccaataatcatctttgttttttaggttcccacctatttatta Find is-nt database================================================ Query_seq: TF0934:TF0936|TF0934:TF0936:hypothetical protein:subtilisin-like serine protease:->->:975268..976007 740 taaattgatggagtttgctttacaaagtacatttgatactcactcataacagatttgttctaatctccttgttcttacatctcgtatatcctgcatcttatacgttttttccgtcggggaagtctttttcgtttcagtggcgtaaaaaaaggagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtcgtaacggcttgccgctccgaccttttttgtccgtgaaagcccaaaactacctttgtcactgacaacgaacaaccgagcccgacatgatatacgggcataagtggaggatgtgcagggagagaagcaggacaaaacaaaaaacacagtctttaaggactcattttatcataagaatagaatacacaaaaacaaaaagctgccccaatgacggcttaaggaaaaaatcaagagagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgtaaagcgttgtatatcattacggaagataggtcgcccaatcattacctatagcattttataactcattaggcattgaataccaatcatttgaaccaaaatgatagattttacggagacagtttttctctatccatattttgtgtcaacccatataatacaagaactgcgatttatcgggtaagccaataatcatctttgttttttaggttcccacctatttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0934:TF0936|TF0934:TF0936:hypothetical protein:subtilisin-like serine protease:->->:975268..976007 740 taaattgatggagtttgctttacaaagtacatttgatactcactcataacagatttgttctaatctccttgttcttacatctcgtatatcctgcatcttatacgttttttccgtcggggaagtctttttcgtttcagtggcgtaaaaaaaggagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtcgtaacggcttgccgctccgaccttttttgtccgtgaaagcccaaaactacctttgtcactgacaacgaacaaccgagcccgacatgatatacgggcataagtggaggatgtgcagggagagaagcaggacaaaacaaaaaacacagtctttaaggactcattttatcataagaatagaatacacaaaaacaaaaagctgccccaatgacggcttaaggaaaaaatcaagagagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgtaaagcgttgtatatcattacggaagataggtcgcccaatcattacctatagcattttataactcattaggcattgaataccaatcatttgaaccaaaatgatagattttacggagacagtttttctctatccatattttgtgtcaacccatataatacaagaactgcgatttatcgggtaagccaataatcatctttgttttttaggttcccacctatttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0934:TF0936|TF0934:TF0936:hypothetical protein:subtilisin-like serine protease:->->:975268..976007 740 taaattgatggagtttgctttacaaagtacatttgatactcactcataacagatttgttctaatctccttgttcttacatctcgtatatcctgcatcttatacgttttttccgtcggggaagtctttttcgtttcagtggcgtaaaaaaaggagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtcgtaacggcttgccgctccgaccttttttgtccgtgaaagcccaaaactacctttgtcactgacaacgaacaaccgagcccgacatgatatacgggcataagtggaggatgtgcagggagagaagcaggacaaaacaaaaaacacagtctttaaggactcattttatcataagaatagaatacacaaaaacaaaaagctgccccaatgacggcttaaggaaaaaatcaagagagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgtaaagcgttgtatatcattacggaagataggtcgcccaatcattacctatagcattttataactcattaggcattgaataccaatcatttgaaccaaaatgatagattttacggagacagtttttctctatccatattttgtgtcaacccatataatacaagaactgcgatttatcgggtaagccaataatcatctttgttttttaggttcccacctatttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaattgatggagtttgctttacaaagtacatttgatactcactcataacagatttgttctaatctccttgttcttacatctcgtatatcctgcatcttatacgttttttccgtcggggaagtctttttcgtttcagtggcgtaaaaaaaggagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtcgtaacggcttgccgctccgaccttttttgtccgtgaaagcccaaaactacctttgtcactgacaacgaacaaccgagcccgacatgatatacgggcataagtggaggatgtgcagggagagaagcaggacaaaacaaaaaacacagtctttaaggactcattttatcataagaatagaatacacaaaaacaaaaagctgccccaatgacggcttaaggaaaaaatcaagagagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgtaaagcgttgtatatcattacggaagataggtcgcccaatcattacctatagcattttataactcattaggcattgaataccaatcatttgaaccaaaatgatagattttacggagacagtttttctctatccatattttgtgtcaacccatataatacaagaactgcgatttatcgggtaagccaataatcatctttgttttttaggttcccacctatttatta Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 53 End: 142 .................................................... M C S N L L V L T S R I S C I L Y V F S V G E V F F V S V A ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 48 Strand: + Start: 15 End: 158 .............. M L Y K V H L I L T H N R F V L I S L F L H L V Y P A S Y T F F P S G K S F S F Q W R K K R R A ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 40 Strand: + Start: 79 End: 198 .............................................................................. M S Y I L H L I R F F R R G S L F R F S G V K K G G L R T N K V L R R E Y Y L Q .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 33 Strand: + Start: 186 End: 284 ......................................................................................................................................................................................... M L P A M K W K C G D C R R N G L P L R P F L S V K A Q N Y L C H ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 35 Strand: + Start: 326 End: 430 ..................................................................................................................................................................................................................................................................................................................................... M E D V Q G E K Q D K T K N T V F K D S F Y H K N R I H K N K K L P Q ...................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 309 End: 515 .................................................................................................................................................................................................................................................................................................................... M I Y G H K W R M C R E R S R T K Q K T Q S L R T H F I I R I E Y T K T K S C P N D G L R K K S R E K I L F S H D I P L ................................................................................................................................................................................................................................. Protein_Len: 42 Strand: + Start: 449 End: 574 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M K R E N S F F S R Y T F I F A T G Y S S Y V K R C I S L R K I G R P I I T Y S I L ...................................................................................................................................................................... Protein_Len: 38 Strand: + Start: 589 End: 702 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M N T N H L N Q N D R F Y G D S F S L S I F C V N P Y N T R T A I Y R V S Q ...................................... Protein_Len: 32 Strand: - Start: 394 End: 489 ......................................................................................................................................................................................................................................................................................................................................................................................................... L F L I C L F L F S G W H R S L S F I L L S F E K K E R Y V K M ........................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 192 End: 371 ............................................................................................................................................................................................... R C H F P L T S I T T T V A Q R E S R K Q G H F G L V V K T V S L S C G L G V H Y V P M L P P H A P L S A P C F L F C M ................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 178 End: 369 ................................................................................................................................................................................. V Q L S I S T H L N D D Y R S A A G V K K T R S L G F S G K D S V V F L R A R C S I R A Y T S S T C P S F C S L V F F M ................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: - Start: 78 End: 170 ............................................................................. M E Y I R C R I R K K R R P L R K R K L P T F F P P S L V F M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... taaattgatggagtttgctttacaaagtacatttgatactcactcataacagatttgttctaatctccttgttcttacatctcgtatatcctgcatcttatacgttttttccgtcggggaagtctttttcgtttcagtggcgtaaaaaaaggagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtcgtaacggcttgccgctccgaccttttttgtccgtgaaagcccaaaactacctttgtcactgacaacgaacaaccgagcccgacatgatatacgggcataagtggaggatgtgcagggagagaagcaggacaaaacaaaaaacacagtctttaaggactcattttatcataagaatagaatacacaaaaacaaaaagctgccccaatgacggcttaaggaaaaaatcaagagagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgtaaagcgttgtatatcattacggaagataggtcgcccaatcattacctatagcattttataactcattaggcattgaataccaatcatttgaaccaaaatgatagattttacggagacagtttttctctatccatattttgtgtcaacccatataatacaagaactgcgatttatcgggtaagccaataatcatctttgttttttaggttcccacctatttatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaattgatggagtttgctttacaaagtacatttgatactcactcataacagatttgttctaatctccttgttcttacatctcgtatatcctgcatcttatacgttttttccgtcggggaagtctttttcgtttcagtggcgtaaaaaaaggagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtcgtaacggcttgccgctccgaccttttttgtccgtgaaagcccaaaactacctttgtcactgacaacgaacaaccgagcccgacatgatatacgggcataagtggaggatgtgcagggagagaagcaggacaaaacaaaaaacacagtctttaaggactcattttatcataagaatagaatacacaaaaacaaaaagctgccccaatgacggcttaaggaaaaaatcaagagagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgtaaagcgttgtatatcattacggaagataggtcgcccaatcattacctatagcattttataactcattaggcattgaataccaatcatttgaaccaaaatgatagattttacggagacagtttttctctatccatattttgtgtcaacccatataatacaagaactgcgatttatcgggtaagccaataatcatctttgttttttaggttcccacctatttatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 75 HP_score: -3.1 Tail_Score: -5.28176 Start: 222 End: 247 Full_Region: AAGTGTGGAGATTGT CGTCGTAACGG CTTG CCGCTCCGACC TTTTTTGTCCGTGAA .............................................................................................................................................................................................................................CGTCGTAACGGCTTGCCGCTCCGACC............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 76 HP_score: -3.9 Tail_Score: -5.26875 Start: 450 End: 478 Full_Region: aaagataaaggtata tcgtgagaaaaaa gaa ttttctctcttga ttttttccttaagcc .................................................................................................................................................................................................................................................................................................................................................................................................................................................................tcgtgagaaaaaagaattttctctcttga...................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 62 HP_score: -6.1 Tail_Score: -3.55478 Start: 456 End: 474 Full_Region: ataaaggtatatcgt gagaaaa aagaa ttttctc tcttgattttttcct .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................gagaaaaaagaattttctc.......................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 43 HP_score: -2.5 Tail_Score: -3.04398 Start: 597 End: 620 Full_Region: TTAGGCATTGAATAC CAATCATTT GAACCA AAATGATAG ATTTTACGGAGACAG ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CAATCATTTGAACCAAAATGATAG........................................................................................................................ Find igs database================================================ Query_seq: TF0934:TF0936|TF0934:TF0936:hypothetical protein:subtilisin-like serine protease:->->:975268..976007 740 taaattgatggagtttgctttacaaagtacatttgatactcactcataacagatttgttctaatctccttgttcttacatctcgtatatcctgcatcttatacgttttttccgtcggggaagtctttttcgtttcagtggcgtaaaaaaaggagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtcgtaacggcttgccgctccgaccttttttgtccgtgaaagcccaaaactacctttgtcactgacaacgaacaaccgagcccgacatgatatacgggcataagtggaggatgtgcagggagagaagcaggacaaaacaaaaaacacagtctttaaggactcattttatcataagaatagaatacacaaaaacaaaaagctgccccaatgacggcttaaggaaaaaatcaagagagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgtaaagcgttgtatatcattacggaagataggtcgcccaatcattacctatagcattttataactcattaggcattgaataccaatcatttgaaccaaaatgatagattttacggagacagtttttctctatccatattttgtgtcaacccatataatacaagaactgcgatttatcgggtaagccaataatcatctttgttttttaggttcccacctatttatta Intra-Species Hit: Count: 1 Min: 1 Max: 740 Len: 740 Subject: tfor_TF0934_TF0936|hypothetical protein:subtilisin-like serine protease|POSITIVE:POSITIVE|[975268,976007]|740 HSP 1 e-value: 0.0 bit: 1425.0 Len: 740 Query Start:1 Query End:740 Subject Strand: POSITIVE Subject Start: 1 Subject End: 740 taaattgatggagtttgctttacaaagtacatttgatactcactcataacagatttgttctaatctccttgttcttacatctcgtatatcctgcatcttatacgttttttccgtcggggaagtctttttcgtttcagtggcgtnnnnnnnggagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtcgtaacggcttgccgctccgaccttttttgtccgtgaaagcccaaaactacctttgtcactgacaacgaacaaccgagcccgacatgatatacgggcataagtggaggatgtgcagggagagaagcaggacaaaacaaaaaacacagtctttaaggactcattttatcataagaatagaatacacaaaaacaaaaagctgccccaatgacggcttaaggaaaaaatcaagagagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgtaaagcgttgtatatcattacggaagataggtcgcccaatcattacctatagcattttataactcattaggcattgaataccaatcatttgaaccaaaatgatagattttacggagacagtttttctctatccatattttgtgtcaacccatataatacaagaactgcgatttatcgggtaagccaataatcatctttgttttttaggttcccacctatttatta taaattgatggagtttgctttacaaagtacatttgatactcactcataacagatttgttctaatctccttgttcttacatctcgtatatcctgcatcttatacgttttttccgtcggggaagtctttttcgtttcagtggcgtaaaaaaaggagggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtcgtaacggcttgccgctccgaccttttttgtccgtgaaagcccaaaactacctttgtcactgacaacgaacaaccgagcccgacatgatatacgggcataagtggaggatgtgcagggagagaagcaggacaaaacaaaaaacacagtctttaaggactcattttatcataagaatagaatacacaaaaacaaaaagctgccccaatgacggcttaaggaaaaaatcaagagagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgtaaagcgttgtatatcattacggaagataggtcgcccaatcattacctatagcattttataactcattaggcattgaataccaatcatttgaaccaaaatgatagattttacggagacagtttttctctatccatattttgtgtcaacccatataatacaagaactgcgatttatcgggtaagccaataatcatctttgttttttaggttcccacctatttatta Inter-species Hit: Count: 3 Min: 153 Max: 587 Len: 435 Subject: pint_PI0282_PI0283|hypothetical protein:Zn-dependent peptidase|POSITIVE:POSITIVE|[266887,267693]|807 HSP 1 e-value: 1.0E-112 bit: 406.0 Len: 435 Query Start:153 Query End:587 Subject Strand: POSITIVE Subject Start: 148 Subject End: 584 ........................................................................................................................................................agggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtcgtaacggcttgccgctccgaccttttttgtccgtgaaagcccaaaactacctttgtcactgacaacgaacaaccgagcccgacatgatatacgggcataagtggaggatgtgcagggagagaagcaggac-aaaacaaaaaacacagtctttaaggact-cattttatcataagaatagaatacac-aaaaacaaaaagctgccccaa-tgacggct-taaggaaaaaatcaagagagaaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgtaaagcgttgtatatcattacggaagataggtcgcccaatcattacctatagcattttataactcattaggc......................................................................................................................................................... ........................................................................................................................................................agggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtctaaacggcttgccgccccgaccttttttgtccgtagaagcccggaactacctttgccgctgacaacgaacaaccgatcccgacataatacacgggcataagtggaggatgtgcagacggagaagcggggcgaaaacaaaaaacgcagtcttcggggactgcatcttatcataa-aacggaatacacggaaaacaaaaagccgccccaacggacggctctatgaaaataatttggag--aaaattcttttttctcacgatatacctttatctttgcaacaggttattcgagttatgcaaagcgttgtatatcactgttgaagataggtcgctaaatcattacctactgcatttcataactcataaggc......................................................................................................................................................... Subject: NC_002950_PG0831_PG0832|hypothetical protein:hypothetical protein|POSITIVE:NEGATIVE|[887972,888769]|798 HSP 1 e-value: 3.0E-76 bit: 287.0 Len: 275 Query Start:153 Query End:427 Subject Strand: POSITIVE Subject Start: 366 Subject End: 641 ........................................................................................................................................................agggcttaggacaaacaaggttttacggcgagaatactacctgcaatgaaatggaagtgtggagattgtcgtcgtaacggcttgccgctccgaccttttttgtccgtgaaagcccaaaactacctttgtcactgacaacgaacaaccgagcccgacatgatatacgggcataagtggaggatgtgcagggagagaagcaggac-aaaacaaaaaacacagtctttaaggactcattttatcataagaatagaatacaca-aaaacaaaaagctgccc......................................................................................................................................................................................................................................................................................................................... ........................................................................................................................................................agggcttaggacaaacaaggttttacggtgagaatactacctgcaataaaatggaagtgtggagattgtcgtctaaacggcttgctgccccgaccttttttgtccgtagaagcctggaactaccttcgccgctgacaacgaacaaccgatcccgacatgatacac-ggcataagttgaggaagtgcagggagagaagcagggcgaacacaaaaaacgcagtctttaaagactgcatttaccataagaatagaatacacagaaaacaaaaagccgccc......................................................................................................................................................................................................................................................................................................................... Subject: NC_002950_PG0835_PG0838|hypothetical protein:integrase|POSITIVE:POSITIVE|[893858,895616]|1759 HSP 1 e-value: 3.0E-17 bit: 91.7 Len: 101 Query Start:396 Query End:496 Subject Strand: POSITIVE Subject Start: 1 Subject End: 102 ...........................................................................................................................................................................................................................................................................................................................................................................................................agaatagaatacaca-aaaacaaaaagctgccccaatgacggcttaaggaaaaaatcaagagagaaaattcttttttctcacgatatacctttatctttgca.................................................................................................................................................................................................................................................... ...........................................................................................................................................................................................................................................................................................................................................................................................................agaatagaatacacagaaaacaaaaagccacccaacggacggctttacaaaaaaatcgtgagagaaaattcttttttctcacgacatccctttatctttgca.................................................................................................................................................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGGGCUUAGGACAAACAAGGUUUUACGGCGAGAAUACUACCUGCAAUGAAAUGGAAGUGUGGAGAUUGUCGUCGUAACGGCUUGCCGCUCCGACCUUUUUUGUCCGUGAAAGCCCAAAACUACCUUUGUCACUGACAACGAACAACCGAGCCCGACAUGAUAUACGGGCAUAAGUGGAGGAUGUGCAGGGAGAGAAGCAGGACAAAACAAAAAACACAGUCUUUAAGGACUCAUUUUAUCAUAAGAAUAGAAUACACAAAAACAAAAAGCUGCCCCAAUGACGGCUUAAGGAAAAAAUCAAGAGAGAAAAUUCUUUUUUCUCACGAUAUACCUUUAUCUUUGCAACAGGUUAUUCGAGUUAUGUAAAGCGUUGUAUAUCAUUACGGAAGAUAGGUCGCCCAAUCAUUACCUAUAGCAUUUUAUAACUCAUUAGGC .((((((.(((((((.((((((.((((((((.(((.((((((.((......))..)).))))..))).)))))))).(((....)))....)))))).)))))))....)))))).......((((((((...))))).(((.((((..(((((..........)))))....(..((((.......(((.(...(((...................(((((....))))).((((((((....).)))))))...............))).))))...........((((.....(((..((((((((.....))))))))..)))...))))..))))..)....)))).)))((((((((.((.((.(((((......)))))...((((((............)))))).)).)).))))))))...))). (-111.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCCUAAUGAGUUAUAAAAUGCUAUAGGUAAUGAUUGGGCGACCUAUCUUCCGUAAUGAUAUACAACGCUUUACAUAACUCGAAUAACCUGUUGCAAAGAUAAAGGUAUAUCGUGAGAAAAAAGAAUUUUCUCUCUUGAUUUUUUCCUUAAGCCGUCAUUGGGGCAGCUUUUUGUUUUUGUGUAUUCUAUUCUUAUGAUAAAAUGAGUCCUUAAAGACUGUGUUUUUUGUUUUGUCCUGCUUCUCUCCCUGCACAUCCUCCACUUAUGCCCGUAUAUCAUGUCGGGCUCGGUUGUUCGUUGUCAGUGACAAAGGUAGUUUUGGGCUUUCACGGACAAAAAAGGUCGGAGCGGCAAGCCGUUACGACGACAAUCUCCACACUUCCAUUUCAUUGCAGGUAGUAUUCUCGCCGUAAAACCUUGUUUGUCCUAAGCCCU (((((((..(((((............))))).)))))))...(((((((..(((......)))..((((..(((.....(((((((((.(((((.......((((...((((.(((((((.....)))))))...)))).....))))...(((....))).)))))..........((((((............(((((((((((((......))))........))))))))).............))))))............(((((..........)))))..))))))))).))).))))...)))))))....((((((....(((((((.(((((....((((....))))((((.(((.(((((....((((.((......)).)))))).))).))).))))..))))).))))))).)))))). (-109.41) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 803 149 101 50 2 tfor:TF3046|5end_hypothetical_protein_3274948..3275178_POSITIVE 422 106 61 53 7 tfor:TF1837|5end_conserved_hypothetical_protein;_possible_outer_membrane_protein_1985480..1985710_POSITIVE 419 198 151 196 131 tfor:TF0763|5end_chromate_transport_protein_809675..809905_POSITIVE 408 92 56 205 172 tfor:TF2918|5end_hypothetical_protein_3114084..3114314_POSITIVE 404 199 152 85 31 tfor:TF0578|5end_ribonuclease_G_607939..608169_POSITIVE 399 92 51 161 105 tfor:TF0819|5end_phosphoglycerate_mutase_878447..878677_POSITIVE 380 95 52 210 174 tfor:TF1444|5end_conserved_hypothetical_protein;_possible_hemin_receptor_1528223..1528453_POSITIVE 375 95 52 187 146 tfor:TF0982|5end_conserved_hypothetical_protein_1038633..1038863_POSITIVE 371 108 62 45 5 tfor:TF1145|5end_anaerobic_C4-dicarboxylate_membrane_transporter_1225799..1226029_POSITIVE 369 99 52 92 47 tfor:TF2457|5end_ABC_transporter,_permease_component,_zinc-binding_protein_2633147..2633377_POSITIVE 368 187 143 163 117 tfor:TF3049|5end_conserved_hypothetical_protein_3276517..3276747_POSITIVE 366 201 165 83 50 tfor:TF1568|5end_H+-transporting_ATP_synthase,_subunit_D_1676341..1676571_POSITIVE 366 97 53 224 175 tfor:TF1498|5end_GTP-binding_protein_1597520..1597750_POSITIVE 363 169 125 190 141 tfor:TF0461|5end_ATP:cob(I)alamin_adenosyltransferase_476990..477220_POSITIVE 363 398 352 126 81 tfor:TF0426|5end_conserved_hypothetical_protein_444728..444958_POSITIVE 362 102 61 173 127 tfor:TF2574|5end_preprotein_translocase_SecY_2743783..2744013_POSITIVE 362 99 51 221 166 tfor:TF1292|5end_sensor_histidine_protein_kinase_1372567..1372797_POSITIVE 362 88 43 57 9 tfor:TF1144|5end_ABC_transporter,_ATP-binding/permease_component_1223527..1223757_POSITIVE 362 198 153 48 1 tfor:TF1112|5end_possible_response_regulator,_LytR/AlgR_family_1182009..1182239_POSITIVE 358 88 41 180 129 tfor:TF2953|5end_possible_TonB-dependent_outer_membrane_receptor_3161143..3161373_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 894 70 21 135 86 tfor:TF3046|3end_hypothetical_protein_3275192..3275342_POSITIVE 399 92 51 151 95 tfor:TF0818|3end_bifunctional_protein:__aspartokinase_I;_homoserine_dehydrogenase_878517..878667_POSITIVE 380 95 52 110 74 tfor:TF1443|3end_hypothetical_protein_1528203..1528353_POSITIVE 372 115 71 53 5 tfor:TF2159|3end_conserved_hypothetical_protein_2336076..2336226_POSITIVE 369 99 52 73 28 tfor:TF2456|3end_ABC_transporter,_ATP-binding_protein_2633208..2633358_POSITIVE 366 201 165 83 50 tfor:TF1567|3end_V-type_ATP_synthase,_subunit_I_1676421..1676571_POSITIVE 366 97 53 143 94 tfor:TF1497|3end_GTP-binding_protein,_Era/ThdF_family_1597519..1597669_POSITIVE 362 189 145 54 3 tfor:TF1948|3end_conserved_hypothetical_protein_2096795..2096945_POSITIVE 362 88 43 51 3 tfor:TF1143|3end_ABC_transporter,_ATP-binding/permease_component_1223601..1223751_POSITIVE 356 97 51 134 75 tfor:TF0766|3end_hypothetical_protein_812256..812406_POSITIVE 353 70 21 92 42 tfor:TF3045|3end_two-component_system_response_regulator_3274633..3274783_POSITIVE 345 120 78 84 36 tfor:TF0075|3end_transcriptional_regulator_83118..83268_POSITIVE 343 170 123 133 79 tfor:TF1145|3end_anaerobic_C4-dicarboxylate_membrane_transporter_1227180..1227330_POSITIVE 341 106 66 81 26 tfor:TF2978|3end_conserved_hypothetical_protein_3190862..3191012_POSITIVE 341 106 61 128 83 tfor:TF2673|3end_conserved_hypothetical_protein_2848272..2848422_POSITIVE 341 229 184 124 79 tfor:TF1916|3end_conserved_hypothetical_protein;_possible_ATPase_2069938..2070088_POSITIVE 339 114 78 45 5 tfor:TF2230|3end_CTn_excision_protein_2400394..2400544_POSITIVE 339 92 51 147 115 tfor:TF0780|3end_alpha-L-arabinofuranosidase,_precursor_A_834441..834591_POSITIVE 337 90 45 148 90 tfor:TF1549|3end_conserved_hypothetical_protein_1654921..1655071_POSITIVE 336 156 120 56 15 tfor:TF2879|3end_tRNA_(guanine-N-1)-methyltransferase_3069008..3069158_POSITIVE