Origin IGS: agtgtcaatctttactctccatccgccttcctttcgtgttattaacccgacgggactatttcgtgatttttgaaactattaaatgtctgactaatatatttttgtcaaaataaaaataacgttatatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatataacgttatttttattttgacaaaaatatattagtcagacatttaatagtttcaaaaatcacgaaatagtcccgtcgggttaataacacgaaaggaaggcggatggagagtaaagattgacact Mask Tandem Repeat Region ================================================ agtgtcaatctttactctccatccgccttcctttcgtgttattaacccgacgggactatttcgtgatttttgaaactattaaatgtctgactaatatatttttgtcaaaataaaaataacgttatatta Find is-nt database================================================ Query_seq: TF0898:TF0899|TF0898:TF0899:hypothetical protein:hypothetical protein:->->:945791..945919 129 agtgtcaatctttactctccatccgccttcctttcgtgttattaacccgacgggactatttcgtgatttttgaaactattaaatgtctgactaatatatttttgtcaaaataaaaataacgttatatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0898:TF0899|TF0898:TF0899:hypothetical protein:hypothetical protein:->->:945791..945919 129 agtgtcaatctttactctccatccgccttcctttcgtgttattaacccgacgggactatttcgtgatttttgaaactattaaatgtctgactaatatatttttgtcaaaataaaaataacgttatatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0898:TF0899|TF0898:TF0899:hypothetical protein:hypothetical protein:->->:945791..945919 129 agtgtcaatctttactctccatccgccttcctttcgtgttattaacccgacgggactatttcgtgatttttgaaactattaaatgtctgactaatatatttttgtcaaaataaaaataacgttatatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agtgtcaatctttactctccatccgccttcctttcgtgttattaacccgacgggactatttcgtgatttttgaaactattaaatgtctgactaatatatttttgtcaaaataaaaataacgttatatta Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 21 End: 110 .................... M R L P F V L L T R R D Y F V I F E T I K C L T N I F L S K ................... agtgtcaatctttactctccatccgccttcctttcgtgttattaacccgacgggactatttcgtgatttttgaaactattaaatgtctgactaatatatttttgtcaaaataaaaataacgttatatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agtgtcaatctttactctccatccgccttcctttcgtgttattaacccgacgggactatttcgtgatttttgaaactattaaatgtctgactaatatatttttgtcaaaataaaaataacgttatatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0898:TF0899|TF0898:TF0899:hypothetical protein:hypothetical protein:->->:945791..945919 129 agtgtcaatctttactctccatccgccttcctttcgtgttattaacccgacgggactatttcgtgatttttgaaactattaaatgtctgactaatatatttttgtcaaaataaaaataacgttatatta Intra-Species Hit: Count: 1 Min: 1 Max: 129 Len: 129 Subject: tfor_TF0898_TF0899|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[945791,945919]|129 HSP 1 e-value: 2.0E-67 bit: 256.0 Len: 129 Query Start:1 Query End:129 Subject Strand: POSITIVE Subject Start: 1 Subject End: 129 agtgtcaatctttactctccatccgccttcctttcgtgttattaacccgacgggactatttcgtgatttttgaaactattaaatgtctgactaatatatttttgtcaaaataaaaataacgttatatta agtgtcaatctttactctccatccgccttcctttcgtgttattaacccgacgggactatttcgtgatttttgaaactattaaatgtctgactaatatatttttgtcaaaataaaaataacgttatatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUGUCAAUCUUUACUCUCCAUCCGCCUUCCUUUCGUGUUAUUAACCCGACGGGACUAUUUCGUGAUUUUUGAAACUAUUAAAUGUCUGACUAAUAUAUUUUUGUCAAAAUAAAAAUAACGUUAUAUUA ...((((..(((((..............((((.(((.((.....)).))).))))...(((((.......)))))....)))).)..)))).....(((((((((....)))))))))........... (-16.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAUAACGUUAUUUUUAUUUUGACAAAAAUAUAUUAGUCAGACAUUUAAUAGUUUCAAAAAUCACGAAAUAGUCCCGUCGGGUUAAUAACACGAAAGGAAGGCGGAUGGAGAGUAAAGAUUGACACU .........((((.((((..((((((............)))))).......((.((((....(((.((......(((..(((.((.....)).)))..)))...))))).)))).)))))).))))... (-20.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 378 56 19 154 119 tfor:TF2079|5end_permease_2245585..2245815_POSITIVE 370 67 23 170 123 tfor:TF2712|5end_glycosyltransferase_2885918..2886148_POSITIVE 343 70 21 221 167 tfor:TF1196|5end_acetyl-coenzyme_A_synthetase_1279064..1279294_POSITIVE 336 55 12 112 58 tfor:TF1538|5end_conserved_hypothetical_protein_1643531..1643761_POSITIVE 332 66 22 225 177 tfor:TF1591|5end_surface_antigen_BspA_1706387..1706617_POSITIVE 329 65 22 154 108 tfor:TF1579|5end_hypothetical_protein_1686889..1687119_POSITIVE 326 55 14 139 102 tfor:TF0816|5end_hypothetical_protein_874217..874447_POSITIVE 316 65 22 59 13 tfor:TF0470|5end_hypothetical_protein_487253..487483_POSITIVE 310 50 2 221 171 tfor:TF3022|5end_chorismate_synthase_3246136..3246366_POSITIVE 310 66 21 72 19 tfor:TF2793|5end_possible_L-lysine_2,3-aminomutase_2977661..2977891_POSITIVE 308 66 25 210 169 tfor:TF1593|5end_hypothetical_protein_1708859..1709089_POSITIVE 307 80 31 186 134 tfor:TF2573|5end_50S_ribosomal_protein_L15_2743310..2743540_POSITIVE 300 57 11 204 157 tfor:TF2261|5end_transfer_region-related_protein,_TraM_2430496..2430726_POSITIVE 297 55 16 77 39 tfor:TF2878|5end_conserved_hypothetical_protein;_possible_CAAX_amino_terminal_protease_family_3067304..3067534_POSITIVE 293 55 12 212 172 tfor:TF0302|5end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_328641..328871_POSITIVE 287 56 17 217 180 tfor:TF2611|5end_conserved_hypothetical_protein_2787204..2787434_POSITIVE 286 49 1 68 9 tfor:TF1239|5end_RNA_polymerase_ECF-type_sigma_factor_1323580..1323810_POSITIVE 285 70 23 175 131 tfor:TF0352|5end_transposase_element_371223..371453_POSITIVE 284 49 1 62 12 tfor:TF2159|5end_conserved_hypothetical_protein_2334446..2334676_POSITIVE 284 55 16 226 175 tfor:TF1189|5end_outer_membrane_protein,_TonB_dependent_receptor_1270586..1270816_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 316 65 23 142 92 tfor:TF0350|3end_hypothetical_protein_371101..371251_POSITIVE 307 80 31 130 78 tfor:TF2572|3end_50S_ribosomal_protein_L30_2743334..2743484_POSITIVE 285 70 23 98 54 tfor:TF0351|3end_hypothetical_protein_371226..371376_POSITIVE 285 67 24 110 53 tfor:TF0008|3end_hypothetical_protein_4336..4486_POSITIVE 284 49 1 120 70 tfor:TF2158|3end_conserved_hypothetical_protein_2334584..2334734_POSITIVE 280 55 25 132 94 tfor:TF2767|3end_2-oxoglutarate_synthase,_alpha_subunit_2954151..2954301_POSITIVE 277 39 3 63 22 tfor:TF2280|3end_hypothetical_protein_2439474..2439624_POSITIVE 277 65 23 138 76 tfor:TF2119|3end_conserved_hypothetical_protein_2292188..2292338_POSITIVE 277 64 22 88 33 tfor:TF1488|3end_conserved_hypothetical_protein_1588975..1589125_POSITIVE 275 66 23 146 109 tfor:TF2863|3end_conserved_hypothetical_protein_3056543..3056693_POSITIVE 273 56 15 124 81 tfor:TF2654|3end_arginyl-tRNA_synthetase_2824559..2824709_POSITIVE 272 54 15 124 72 tfor:TF2950|3end_chloromuconate_cycloisomerase_3158549..3158699_POSITIVE 272 67 22 120 78 tfor:TF1851|3end_methionine_synthase_I_2004681..2004831_POSITIVE 272 57 17 124 93 tfor:TF1151|3end_conserved_hypothetical_protein_1233262..1233412_POSITIVE 271 70 23 54 14 tfor:TF2789|3end_enoyl-[acyl-carrier-protein]_reductase_2973365..2973515_POSITIVE 266 64 24 68 33 tfor:TF2539|3end_hypothetical_protein_2723116..2723266_POSITIVE 266 41 14 90 49 tfor:TF1301|3end_hypothetical_protein_1383487..1383637_POSITIVE 264 69 22 75 24 tfor:TF1160|3end_MutT/nudix_family_protein_1241008..1241158_POSITIVE 260 55 17 124 68 tfor:TF0242|3end_conserved_hypothetical_protein_274069..274219_POSITIVE 259 58 16 107 54 tfor:TF3126|3end_glycosyltransferase,_group_2_family_3361685..3361835_POSITIVE