Origin IGS: taacacatcgcccttacaatcaaatcatttcgtctattttctatacgttacgtatctaactgacttttttattgtatttttgcacaataagaacaaaataatgtaatcgtc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gacgattacattattttgttcttattgtgcaaaaatacaataaaaaagtcagttagatacgtaacgtatagaaaatagacgaaatgatttgattgtaagggcgatgtgtta Mask Tandem Repeat Region ================================================ taacacatcgcccttacaatcaaatcatttcgtctattttctatacgttacgtatctaactgacttttttattgtatttttgcacaataagaacaaaataatgtaatcgtc Find is-nt database================================================ Query_seq: TF0839:TF0840|TF0839:TF0840:aminopeptidasem M28 family:hypothetical protein:->->:891590..891700 111 taacacatcgcccttacaatcaaatcatttcgtctattttctatacgttacgtatctaactgacttttttattgtatttttgcacaataagaacaaaataatgtaatcgtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0839:TF0840|TF0839:TF0840:aminopeptidasem M28 family:hypothetical protein:->->:891590..891700 111 taacacatcgcccttacaatcaaatcatttcgtctattttctatacgttacgtatctaactgacttttttattgtatttttgcacaataagaacaaaataatgtaatcgtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0839:TF0840|TF0839:TF0840:aminopeptidasem M28 family:hypothetical protein:->->:891590..891700 111 taacacatcgcccttacaatcaaatcatttcgtctattttctatacgttacgtatctaactgacttttttattgtatttttgcacaataagaacaaaataatgtaatcgtc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacacatcgcccttacaatcaaatcatttcgtctattttctatacgttacgtatctaactgacttttttattgtatttttgcacaataagaacaaaataatgtaatcgtc Predict ORF larger than 30AA ================================================ taacacatcgcccttacaatcaaatcatttcgtctattttctatacgttacgtatctaactgacttttttattgtatttttgcacaataagaacaaaataatgtaatcgtc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacacatcgcccttacaatcaaatcatttcgtctattttctatacgttacgtatctaactgacttttttattgtatttttgcacaataagaacaaaataatgtaatcgtc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0839:TF0840|TF0839:TF0840:aminopeptidasem M28 family:hypothetical protein:->->:891590..891700 111 taacacatcgcccttacaatcaaatcatttcgtctattttctatacgttacgtatctaactgacttttttattgtatttttgcacaataagaacaaaataatgtaatcgtc Intra-Species Hit: Count: 1 Min: 1 Max: 111 Len: 111 Subject: tfor_TF0839_TF0840|aminopeptidasem M28 family:hypothetical protein|POSITIVE:POSITIVE|[891590,891700]|111 HSP 1 e-value: 7.0E-57 bit: 220.0 Len: 111 Query Start:1 Query End:111 Subject Strand: POSITIVE Subject Start: 1 Subject End: 111 taacacatcgcccttacaatcaaatcatttcgtctattttctatacgttacgtatctaactgacttttttattgtatttttgcacaataagaacaaaataatgtaatcgtc taacacatcgcccttacaatcaaatcatttcgtctattttctatacgttacgtatctaactgacttttttattgtatttttgcacaataagaacaaaataatgtaatcgtc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACACAUCGCCCUUACAAUCAAAUCAUUUCGUCUAUUUUCUAUACGUUACGUAUCUAACUGACUUUUUUAUUGUAUUUUUGCACAAUAAGAACAAAAUAAUGUAAUCGUC .........((..(((((.............(((..((....(((((...)))))..))..))).((((((((((........))))))))))........)))))..)). (-12.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GACGAUUACAUUAUUUUGUUCUUAUUGUGCAAAAAUACAAUAAAAAAGUCAGUUAGAUACGUAACGUAUAGAAAAUAGACGAAAUGAUUUGAUUGUAAGGGCGAUGUGUUA .(((((((.(((((((((((.((((((((......)))))))).........((..(((((...)))))..))....))))))))))).)))))))............... (-21.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 252 69 26 101 55 tfor:TF2046|5end_polyribonucleotide_nucleotidyltransferase_2207820..2208050_POSITIVE 243 70 22 108 61 tfor:TF2317|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_II_2474418..2474648_POSITIVE 237 83 41 101 52 tfor:TF3005|5end_histidine_kinase_sensor_protein_3224805..3225035_POSITIVE 234 70 21 62 14 tfor:TF2472|5end_GAF_domain-containing_protein_2649918..2650148_POSITIVE 217 41 6 189 153 tfor:TF2315|5end_thioesterase_2473996..2474226_POSITIVE 214 47 6 68 33 tfor:TF1800|5end_conserved_hypothetical_protein_1941214..1941444_POSITIVE 214 63 24 189 150 tfor:TF0145|5end_glutamine_synthetase_152446..152676_POSITIVE 211 58 12 130 84 tfor:TF3118|5end_conserved_hypothetical_protein_3352373..3352603_POSITIVE 210 22 2 48 24 tfor:TF2438|5end_hypothetical_protein_2615781..2616011_POSITIVE 204 52 11 52 14 tfor:TF2228|5end_CTn_excision_protein_2395899..2396129_POSITIVE 203 50 5 65 9 tfor:TF2205|5end_conserved_hypothetical_protein;_possible_transcriptional_regulator_2379055..2379285_POSITIVE 203 41 7 227 193 tfor:TF1694|5end_conserved_hypothetical_protein_1812493..1812723_POSITIVE 202 48 10 225 192 tfor:TF2958|5end_type_I_restriction-modification_system_R_subunit_3166573..3166803_POSITIVE 202 23 6 132 113 tfor:TF2272|5end_conserved_hypothetical_protein_2436156..2436386_POSITIVE 201 69 26 161 114 tfor:TF1768|5end_conserved_hypothetical_protein;_possible_transposase_1906526..1906756_POSITIVE 199 76 45 112 81 tfor:TF2931|5end_3-deoxy-D-manno-2-octulosonic_acid_(Kdo_transferase)_3134275..3134505_POSITIVE 196 68 23 211 171 tfor:TF0895|5end_hypothetical_protein_944455..944685_POSITIVE 194 79 37 123 73 tfor:TF2482|5end_hypothetical_protein_2658503..2658733_POSITIVE 194 76 31 95 55 tfor:TF0930|5end_conserved_hypothetical_protein;_possible_prophage_protein_968782..969012_POSITIVE 192 83 46 149 117 tfor:TF2566|5end_50S_ribosomal_protein_L5_2740303..2740533_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 253 45 4 82 35 tfor:TF2615|3end_conserved_hypothetical_protein_2791209..2791359_POSITIVE 247 50 2 85 44 tfor:TF1838|3end_tRNA/rRNA_methyltransferase_1988745..1988895_POSITIVE 243 70 22 108 61 tfor:TF2315|3end_thioesterase_2474498..2474648_POSITIVE 235 75 40 149 107 tfor:TF1690|3end_chaperone_protein_dnaK_1808721..1808871_POSITIVE 223 62 21 116 84 tfor:TF2166|3end_hypothetical_protein_2342362..2342512_POSITIVE 223 88 50 73 38 tfor:TF1876|3end_hypothetical_protein_2030054..2030204_POSITIVE 220 47 7 86 28 tfor:TF2850|3end_endoribonuclease_L-PSP_3038871..3039021_POSITIVE 219 34 2 30 1 tfor:TF2978|3end_conserved_hypothetical_protein_3190862..3191012_POSITIVE 214 47 6 68 33 tfor:TF1799|3end_quinolinate_synthetase_1941294..1941444_POSITIVE 211 58 12 90 44 tfor:TF3116|3end_conserved_hypothetical_protein_3352413..3352563_POSITIVE 206 49 1 45 2 tfor:TF2285|3end_hypothetical_protein_2441024..2441174_POSITIVE 202 48 10 37 4 tfor:TF2956|3end_conserved_hypothetical_protein_3166465..3166615_POSITIVE 202 23 6 83 64 tfor:TF2271|3end_conserved_hypothetical_protein_2436187..2436337_POSITIVE 199 76 45 109 78 tfor:TF2930|3end_glutamyl-tRNA_synthetase_3134352..3134502_POSITIVE 194 79 37 123 73 tfor:TF2481|3end_possible_thiol_protease/hemagglutinin_PrtT_2658583..2658733_POSITIVE 194 76 31 95 55 tfor:TF0929|3end_conserved_hypothetical_protein_968862..969012_POSITIVE 192 83 46 149 117 tfor:TF2565|3end_50S_ribosomal_protein_L24_2740383..2740533_POSITIVE 192 42 5 99 61 tfor:TF1539|3end_conserved_hypothetical_protein_1644341..1644491_POSITIVE 192 62 21 116 84 tfor:TF0362|3end_hypothetical_protein_380199..380349_POSITIVE 192 64 27 69 36 tfor:TF0133|3end_transposase_element_145556..145706_POSITIVE