Origin IGS: ccccccagccccccttcaaaggggggaagagcgaagcgaagggggatgttaccccttcaaagggggaagagcggaagcgaagcgttggggaaaagcaatatccccgttgggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttgggtttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggcaaataaccattaaccattgataatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taattatcaatggttaatggttatttgccgggtacatgattcggcgtgtaccacgcataaccattgaccattgtccgaaagggctgaaagcccgaattatttcagccccatcccgcaagggtggggtatcgacatccggaccttgttcggagggctgaaggcctgaattaaacccaaattacgcgatacacttttcggcgtttaccattaataatggatgtgcgaatgtacgacaatggttacgtcaggcttacagccctcccaacggggatattgcttttccccaacgcttcgcttccgctcttccccctttgaaggggtaacatcccccttcgcttcgctcttcccccctttgaaggggggctgggggg Mask Tandem Repeat Region ================================================ ccccccagccccccttcaaaggggggaagagcgaagcgaagggggatgttaccccttcaaagggggaagagcggaagcgaagcgttggggaaaagcaatatccccgttgggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttgggtttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggcaaataaccattaaccattgataatta Find is-nt database================================================ Query_seq: TF0821:TF0822|TF0821:TF0822:hypothetical protein:hypothetical protein:->->:880256..880626 371 ccccccagccccccttcaaaggggggaagagcgaagcgaagggggatgttaccccttcaaagggggaagagcggaagcgaagcgttggggaaaagcaatatccccgttgggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttgggtttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggcaaataaccattaaccattgataatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0821:TF0822|TF0821:TF0822:hypothetical protein:hypothetical protein:->->:880256..880626 371 ccccccagccccccttcaaaggggggaagagcgaagcgaagggggatgttaccccttcaaagggggaagagcggaagcgaagcgttggggaaaagcaatatccccgttgggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttgggtttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggcaaataaccattaaccattgataatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0821:TF0822|TF0821:TF0822:hypothetical protein:hypothetical protein:->->:880256..880626 371 ccccccagccccccttcaaaggggggaagagcgaagcgaagggggatgttaccccttcaaagggggaagagcggaagcgaagcgttggggaaaagcaatatccccgttgggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttgggtttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggcaaataaccattaaccattgataatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ccccccagccccccttcaaaggggggaagagcgaagcgaagggggatgttaccccttcaaagggggaagagcggaagcgaagcgttggggaaaagcaatatccccgttgggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttgggtttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggcaaataaccattaaccattgataatta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 254 End: 355 ............................................................................................................................................................................................................................................................. M R D G A E I I R A F S P F G Q W S M V M R G T R R I M Y P A N N H ................ Protein_Len: 40 Strand: - Start: 217 End: 336 ........................................................................................................................................................................................................................ G E S C P G S T S V G G K R S P A S I I R A K L G K P C H D I T I R P V R R I M ................................... Protein_Len: 31 Strand: - Start: 209 End: 301 ................................................................................................................................................................................................................ A K L G G F L T R I D I G W G Q P I P S F Y N P S E A R E S M ...................................................................... Protein_Len: 45 Strand: - Start: 60 End: 194 ........................................................... L P F L P L S A N P F L L I G T P L A T L R V Y G N D Y M R V D M I L P L R R F T Y R T M ................................................................................................................................................................................. Protein_Len: 45 Strand: - Start: 52 End: 186 ................................................... G K L P P L A S A F R Q P F A I D G N P P S Y A Q R L W Q R V N A C G N N I T F A S F H M ......................................................................................................................................................................................... Protein_Len: 51 Strand: - Start: 2 End: 154 . G W G G K L P P F L S A F P S T V G E F P S S R F R L T P S F C Y G R Q S P Q L G S T V M T T C E C M ......................................................................................................................................................................................................................... ccccccagccccccttcaaaggggggaagagcgaagcgaagggggatgttaccccttcaaagggggaagagcggaagcgaagcgttggggaaaagcaatatccccgttgggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttgggtttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggcaaataaccattaaccattgataatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ccccccagccccccttcaaaggggggaagagcgaagcgaagggggatgttaccccttcaaagggggaagagcggaagcgaagcgttggggaaaagcaatatccccgttgggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttgggtttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggcaaataaccattaaccattgataatta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 54 HP_score: -7.3 Tail_Score: -2.57132 Start: 303 End: 360 Full_Region: taattatcaat ggttaatggttatttgccgggtacatg attcgg cgtgtaccacgcataaccattgacc attgtccgaaagggc ..............................................................................................................................................................................................................................................................................................................ggttaatggttatttgccgggtacatgattcggcgtgtaccacgcataaccattgacc........... Find igs database================================================ Query_seq: TF0821:TF0822|TF0821:TF0822:hypothetical protein:hypothetical protein:->->:880256..880626 371 ccccccagccccccttcaaaggggggaagagcgaagcgaagggggatgttaccccttcaaagggggaagagcggaagcgaagcgttggggaaaagcaatatccccgttgggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttgggtttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggcaaataaccattaaccattgataatta Intra-Species Hit: Count: 18 Min: 15 Max: 371 Len: 357 Subject: tfor_TF0821_TF0822|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[880256,880626]|371 HSP 1 e-value: 0.0 bit: 708.0 Len: 357 Query Start:15 Query End:371 Subject Strand: POSITIVE Subject Start: 15 Subject End: 371 ..............ttcaaaggggggaagagcgaagcgaagggggatgttaccccttcaaagggggaagagcggaagcgaagcgttggggaaaagcaatatccccgttgggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttgggtttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggcaaataaccattaaccattgataatta ..............ttcaaaggggggaagagcgaagcgaagggggatgttaccccttcaaagggggaagagcggaagcgaagcgttggggaaaagcaatatccccgttgggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttgggtttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggcaaataaccattaaccattgataatta Subject: tfor_TF0370_TF0371|hypothetical protein:beta-mannosidase precursor|POSITIVE:POSITIVE|[386378,386902]|525 HSP 1 e-value: 1.0E-89 bit: 331.0 Len: 186 Query Start:20 Query End:205 Subject Strand: POSITIVE Subject Start: 1 Subject End: 187 ...................aggggggaagagcgaagcgaagggggatgttaccccttcaaagggggaagagcggaagcgaagcgttggggaaaa-gcaatatccccgttgggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttgggtttaat...................................................................................................................................................................... ...................aggggggaagagcgaagcgaagggggatgttaccccttcaaagggggaagagcggaagcgaagcgttggggaaaaagcaatatctccgttgggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattgttaatggtaaacgccgaacagtgtatcgcgtaatttgggtttaat...................................................................................................................................................................... Subject: tfor_TF0677_TF0678|ATP-binding protein, Mrp/Nbp35 family, involved in chromosome partitioning:hypothetical protein|POSITIVE:POSITIVE|[721043,721242]|200 HSP 1 e-value: 9.0E-78 bit: 291.0 Len: 155 Query Start:200 Query End:354 Subject Strand: POSITIVE Subject Start: 43 Subject End: 197 .......................................................................................................................................................................................................tttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggcaaataacca................. .......................................................................................................................................................................................................tttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatagggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgtggtacacgccgaatcatgtacccggcaaataacca................. Subject: tfor_TF2190_TF2191|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2365707,2366073]|367 HSP 1 e-value: 1.0E-76 bit: 287.0 Len: 157 Query Start:200 Query End:356 Subject Strand: POSITIVE Subject Start: 165 Subject End: 321 .......................................................................................................................................................................................................tttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggcaaataaccatt............... .......................................................................................................................................................................................................tttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgcggtacactccgaatcatgtacccggcaaataaccatt............... Subject: tfor_TF3003_TF3004|hypothetical protein:phosphoribosylformylglycinamidine (FGAM) synthase|POSITIVE:POSITIVE|[3220690,3220958]|269 HSP 1 e-value: 8.0E-69 bit: 262.0 Len: 136 Query Start:200 Query End:335 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 136 .......................................................................................................................................................................................................tttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatca.................................... .......................................................................................................................................................................................................tttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggttaatggttatgcgtggtacacgccgaatca.................................... Subject: tfor_TF1473_TF1474|hypothetical protein:6- phosphogluconolactonase|POSITIVE:POSITIVE|[1567027, 1567481]|455 HSP 1 e-value: 2.0E-47 bit: 190.0 Len: 147 Query Start:200 Query End:346 Subject Strand: NEGATIVE Subject Start: 210 Subject End: 349 .......................................................................................................................................................................................................tttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggca......................... .......................................................................................................................................................................................................tttaattcaggccttcagcccttcgaacaaggtccggatgtcgatcccccacccttacgggatggggctgaaataattcgggctttcagccctttcg------ggt-aatggttatgcgtgttacacgctgaatcatgtacccggca......................... HSP 2 e-value: 7.0E-26 bit: 119.0 Len: 92 Query Start:110 Query End:201 Subject Strand: NEGATIVE Subject Start: 32 Subject End: 123 .............................................................................................................ggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttgggtt.......................................................................................................................................................................... .............................................................................................................ggagggctgtaagcctgacgtaaccattgtcgtacattcatacgtccattattaatggaaaacgcccaacagtatatcgcgtgatttgggtt.......................................................................................................................................................................... Subject: tfor_TF2047_TF2048|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2210359,2210782]|424 HSP 1 e-value: 8.0E-38 bit: 159.0 Len: 93 Query Start:200 Query End:292 Subject Strand: NEGATIVE Subject Start: 264 Subject End: 358 .......................................................................................................................................................................................................tttaattcaggccttcagccctccgaacaaggtccggatgtcgata--ccccacccttgcgggatggggctgaaataattcgggctttcagccct............................................................................... .......................................................................................................................................................................................................tttaattcaggctttcagccctccgaacaaggtccggatgtcgatataccccacccttgcgggatggggctgaaataattcgggctttcagccct............................................................................... HSP 2 e-value: 7.0E-29 bit: 129.0 Len: 85 Query Start:110 Query End:194 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 85 .............................................................................................................ggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaat................................................................................................................................................................................. .............................................................................................................ggagggctgtaagcctgacgtaaccattgtcgtgcattcgtacgtccatgattaatggtaaacgccgaacagtgtatcgcgtaat................................................................................................................................................................................. Subject: tfor_TF2025_TF2026|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2182241,2182798]|558 HSP 1 e-value: 3.0E-37 bit: 157.0 Len: 166 Query Start:200 Query End:365 Subject Strand: NEGATIVE Subject Start: 27 Subject End: 202 .......................................................................................................................................................................................................tttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccaccctt--gcgggatggggctgaaataattcgggc-tttcagccctttcggacaatggtcaatg-------gttatgcgtggtacacgccgaatcatgtacccggcaaataaccattaaccattga...... .......................................................................................................................................................................................................tttaattcaggccttcagccctccgaacaaggtctggatatcggtatcccacccttgcgcgggatggggctgaaataactagggcttttcagccctttcggacaatagtcaatggttaattgttatgcgtggaacacgccgaaccatgtatttggaaaataaccattaaccattga...... Subject: tfor_TF2201_TF2202|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2378345,2378821]|477 HSP 1 e-value: 8.0E-32 bit: 139.0 Len: 125 Query Start:77 Query End:201 Subject Strand: NEGATIVE Subject Start: 212 Subject End: 336 ............................................................................gcgaagcgttggggaaaa-gcaatatccccgttgggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttgggtt.......................................................................................................................................................................... ............................................................................gcgaagcgttggggaaaaagcaatatccc-gttgggagggctgtaagcctgacataaccattgtcatacatttgcacatccattattaatggtaaacaccaaacagggggtcgcataatttgggtt.......................................................................................................................................................................... HSP 2 e-value: 8.0E-32 bit: 139.0 Len: 74 Query Start:273 Query End:346 Subject Strand: NEGATIVE Subject Start: 404 Subject End: 477 ................................................................................................................................................................................................................................................................................taattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggca......................... ................................................................................................................................................................................................................................................................................taattcgggctttcagccctttcggacaatggttaatggttatgcgtggtacacgccgaatcatgtacccggca......................... Subject: tfor_TF0029_TF0030|hypothetical protein:N-acetylneuraminate lyase|POSITIVE:POSITIVE|[25123,25909]|787 HSP 1 e-value: 8.0E-32 bit: 139.0 Len: 90 Query Start:110 Query End:199 Subject Strand: POSITIVE Subject Start: 454 Subject End: 543 .............................................................................................................ggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttggg............................................................................................................................................................................ .............................................................................................................ggagggctgtaagcttgacgtaaccattgtcgtacattcgtacgtccattattaatggtaaacaccgaacagtgtatcgcgtaatttggg............................................................................................................................................................................ HSP 2 e-value: 6.0E-14 bit: 79.8 Len: 48 Query Start:246 Query End:293 Subject Strand: POSITIVE Subject Start: 213 Subject End: 260 .....................................................................................................................................................................................................................................................ccccacccttgcgggatggggctgaaataattcgggctttcagccctt.............................................................................. .....................................................................................................................................................................................................................................................ccccacccttacgggatggggctgaaataattcgggcttccagccctt.............................................................................. Subject: tfor_TF0467_TF0468|acetyltransferase-related protein:hypothetical protein|POSITIVE:POSITIVE|[486604,487068]|465 HSP 1 e-value: 1.0E-30 bit: 135.0 Len: 80 Query Start:120 Query End:199 Subject Strand: NEGATIVE Subject Start: 24 Subject End: 103 .......................................................................................................................aagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttggg............................................................................................................................................................................ .......................................................................................................................aagcctgacgtaaccattgtcgtacattcgcacgtccattattaatggtaaacgccgaacaatgtatcgcgtaatttggg............................................................................................................................................................................ HSP 2 e-value: 4.0E-15 bit: 83.8 Len: 46 Query Start:311 Query End:356 Subject Strand: NEGATIVE Subject Start: 396 Subject End: 441 ......................................................................................................................................................................................................................................................................................................................gttatgcgtggtacacgccgaatcatgtacccggcaaataaccatt............... ......................................................................................................................................................................................................................................................................................................................gttatgcgtggtacacgccgaatcatgtacccagcaaataaccatt............... HSP 3 e-value: 2.0E-11 bit: 71.9 Len: 43 Query Start:52 Query End:94 Subject Strand: NEGATIVE Subject Start: 132 Subject End: 175 ...................................................ccccttcaaaggggg-aagagcggaagcgaagcgttggggaaaa..................................................................................................................................................................................................................................................................................... ...................................................ccccttcaaaggggggaagagcggaagcgaagcgttggggaaaa..................................................................................................................................................................................................................................................................................... Subject: tfor_TF0585_TF0586|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[616824,617452]|629 HSP 1 e-value: 3.0E-28 bit: 127.0 Len: 92 Query Start:110 Query End:201 Subject Strand: POSITIVE Subject Start: 435 Subject End: 526 .............................................................................................................ggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattattaatggtaaacgccgaaaagtgtatcgcgtaatttgggtt.......................................................................................................................................................................... .............................................................................................................ggagggctgtaagcctgacgtaacccttgtcgtacattcgtacgtccattattaatggtaaacgcccaatagggtaccgcgtaatttgggtt.......................................................................................................................................................................... HSP 2 e-value: 1.0E-18 bit: 95.6 Len: 147 Query Start:200 Query End:346 Subject Strand: POSITIVE Subject Start: 213 Subject End: 352 .......................................................................................................................................................................................................tttaattcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctttcggacaatggtcaatggttatgcgtggtacacgccgaatcatgtacccggca......................... .......................................................................................................................................................................................................tttaattcaggccttcagccctctaaccaagatatgggcatggatcccccacccttacgggatggggctgaaatatatctggctttcagccctttcg------ggt-aatggttatgtgtgttacacgccgaatcatgtacccggca......................... Subject: tfor_TF1585_TF1586|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1690671,1691110]|440 HSP 1 e-value: 4.0E-21 bit: 103.0 Len: 60 Query Start:300 Query End:359 Subject Strand: NEGATIVE Subject Start: 110 Subject End: 169 ...........................................................................................................................................................................................................................................................................................................aatggtcaatggttatgcgtggtacacgccgaatcatgtacccggcaaataaccattaac............ ...........................................................................................................................................................................................................................................................................................................aatggtcaattgttatgcgtggtacacgtcgaatcatgtacccggcaaataaccattaac............ HSP 2 e-value: 4.0E-15 bit: 83.8 Len: 54 Query Start:240 Query End:293 Subject Strand: NEGATIVE Subject Start: 198 Subject End: 251 ...............................................................................................................................................................................................................................................tcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctt.............................................................................. ...............................................................................................................................................................................................................................................tcgataccccacccttgcgggatggggctgaaatatatcgggcttgcagccctt.............................................................................. Subject: tfor_TF0179_TF0180|pyrophosphate-energized proton pump:carboxynorspermidine decarboxylase|POSITIVE:POSITIVE|[190752,191406]|655 HSP 1 e-value: 2.0E-20 bit: 101.0 Len: 90 Query Start:110 Query End:199 Subject Strand: NEGATIVE Subject Start: 132 Subject End: 226 .............................................................................................................ggagggctgtaagcctgacgtaaccattgtcgtacattcgcacatccattatt-----aatggtaaacgccgaaaagtgtatcgcgtaatttggg............................................................................................................................................................................ .............................................................................................................ggagggctgtaagcctgacgtaacccttgtcgtacattcgtacgtacattattaatgtaatggtaaacgcccaacagtgtatcgcgtaatttggg............................................................................................................................................................................ HSP 2 e-value: 2.0E-11 bit: 71.9 Len: 40 Query Start:307 Query End:346 Subject Strand: NEGATIVE Subject Start: 316 Subject End: 355 ..................................................................................................................................................................................................................................................................................................................aatggttatgcgtggtacacgccgaatcatgtacccggca......................... ..................................................................................................................................................................................................................................................................................................................aatggttatgcgtgttacacgccgaatcatgtacccggca......................... Subject: tfor_TF1846_TF1847|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1997076,1997528]|453 HSP 1 e-value: 1.0E-15 bit: 85.7 Len: 47 Query Start:22 Query End:68 Subject Strand: POSITIVE Subject Start: 231 Subject End: 277 .....................gggggaagagcgaagcgaagggggatgttaccccttcaaagggggaa............................................................................................................................................................................................................................................................................................................... .....................gggggaagagcgaagcgaagggggatgttaccccttccaagggggaa............................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-11 bit: 71.9 Len: 40 Query Start:300 Query End:339 Subject Strand: POSITIVE Subject Start: 94 Subject End: 133 ...........................................................................................................................................................................................................................................................................................................aatggtcaatggttatgcgtggtacacgccgaatcatgta................................ ...........................................................................................................................................................................................................................................................................................................aatggttaatggttatgcgtggtacacgccgaatcatgta................................ Subject: tfor_TF1483_TF1484|glycosyltransferase family protein:penicillin binding protein|POSITIVE:POSITIVE|[1578886,1579698]|813 HSP 1 e-value: 2.0E-14 bit: 81.8 Len: 52 Query Start:15 Query End:66 Subject Strand: POSITIVE Subject Start: 85 Subject End: 137 ..............ttcaaaggggggaagagcgaagcgaa-gggggatgttaccccttcaaaggggg................................................................................................................................................................................................................................................................................................................. ..............ttcaaaggggggaagagcgaagcgaaggggggatgttatcccttcaaaggggg................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 2.0E-11 bit: 71.9 Len: 40 Query Start:307 Query End:346 Subject Strand: NEGATIVE Subject Start: 530 Subject End: 569 ..................................................................................................................................................................................................................................................................................................................aatggttatgcgtggtacacgccgaatcatgtacccggca......................... ..................................................................................................................................................................................................................................................................................................................aatggttatgcgtgttacacgccgaatcatgtacccggca......................... Subject: tfor_TF2920_TF2921|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[3117231,3118637]|1407 HSP 1 e-value: 2.0E-11 bit: 71.9 Len: 88 Query Start:206 Query End:293 Subject Strand: NEGATIVE Subject Start: 1025 Subject End: 1112 .............................................................................................................................................................................................................tcaggccttcagccctccgaacaaggtccggatgtcgataccccacccttgcgggatggggctgaaataattcgggctttcagccctt.............................................................................. .............................................................................................................................................................................................................tcaggctttcagccctcccaacggggtcggggaatcgataccccatccttacgggatggggctgaaatataccgggctttcagccctt.............................................................................. HSP 2 e-value: 2.0E-7 bit: 58.0 Len: 47 Query Start:22 Query End:68 Subject Strand: NEGATIVE Subject Start: 105 Subject End: 153 .....................gggggaagagcgaa-gcgaagggggatgtta-ccccttcaaagggggaa............................................................................................................................................................................................................................................................................................................... .....................gggggaagagcgaaagcgaagggggatgttatccccctcaaagggggaa............................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 4.0E-6 bit: 54.0 Len: 31 Query Start:52 Query End:82 Subject Strand: NEGATIVE Subject Start: 133 Subject End: 163 ...................................................ccccttcaaagggggaagagcggaagcgaag................................................................................................................................................................................................................................................................................................. ...................................................ccccttcaaagggggaagagcgaaagcgaag................................................................................................................................................................................................................................................................................................. HSP 4 e-value: 4.0E-6 bit: 54.0 Len: 31 Query Start:52 Query End:82 Subject Strand: NEGATIVE Subject Start: 666 Subject End: 696 ...................................................ccccttcaaagggggaagagcggaagcgaag................................................................................................................................................................................................................................................................................................. ...................................................ccccttcaaagggggaaaagcggaagcgaag................................................................................................................................................................................................................................................................................................. Subject: tfor_TF2922_TF2923|surface antigen BspA:hypothetical protein|POSITIVE:POSITIVE|[3121599,3122307]|709 HSP 1 e-value: 4.0E-9 bit: 63.9 Len: 40 Query Start:300 Query End:339 Subject Strand: NEGATIVE Subject Start: 378 Subject End: 417 ...........................................................................................................................................................................................................................................................................................................aatggtcaatggttatgcgtggtacacgccgaatcatgta................................ ...........................................................................................................................................................................................................................................................................................................aatggttaattgttatgcgtggtacacgccgaatcatgta................................ HSP 2 e-value: 1.0E-8 bit: 61.9 Len: 43 Query Start:52 Query End:94 Subject Strand: NEGATIVE Subject Start: 55 Subject End: 97 ...................................................ccccttcaaagggggaagagcggaagcgaagcgttggggaaaa..................................................................................................................................................................................................................................................................................... ...................................................ccccttcaaaggggggaatgcggaagcgaagcgttggggaaaa..................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCAAAGGGGGGAAGAGCGAAGCGAAGGGGGAUGUUACCCCUUCAAAGGGGGAAGAGCGGAAGCGAAGCGUUGGGGAAAAGCAAUAUCCCCGUUGGGAGGGCUGUAAGCCUGACGUAACCAUUGUCGUACAUUCGCACAUCCAUUAUUAAUGGUAAACGCCGAAAAGUGUAUCGCGUAAUUUGGGUUUAAUUCAGGCCUUCAGCCCUCCGAACAAGGUCCGGAUGUCGAUACCCCACCCUUGCGGGAUGGGGCUGAAAUAAUUCGGGCUUUCAGCCCUUUCGGACAAUGGUCAAUGGUUAUGCGUGGUACACGCCGAAUCAUGUACCCGGCAAAUAACCAUUAACCAUUGAUAAUUA (((((((((..(((.........(((((((.......)))))))....(((((...((....))...((...........))....)))))(((.(((((((((...((((((...((((..(((.((((((((((....((((.....)))).....).)))...)))))).))).......))))....))))))...))))))))).)))..(((((((((.(((...((((((((....))).))))).)))....))))))))))))..)))))).)))(((((((.(((((((((.((.((((((.(......).))))))))....))))))))).)))))))....... (-140.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUAUCAAUGGUUAAUGGUUAUUUGCCGGGUACAUGAUUCGGCGUGUACCACGCAUAACCAUUGACCAUUGUCCGAAAGGGCUGAAAGCCCGAAUUAUUUCAGCCCCAUCCCGCAAGGGUGGGGUAUCGACAUCCGGACCUUGUUCGGAGGGCUGAAGGCCUGAAUUAAACCCAAAUUACGCGAUACACUUUUCGGCGUUUACCAUUAAUAAUGGAUGUGCGAAUGUACGACAAUGGUUACGUCAGGCUUACAGCCCUCCCAACGGGGAUAUUGCUUUUCCCCAACGCUUCGCUUCCGCUCUUCCCCCUUUGAAGGGGUAACAUCCCCCUUCGCUUCGCUCUUCCCCCCUUUGAA .......((((((((((((((((..(((..((((((((......))))))))..)))))))))))))))))))...((..(((((((((..........)))))))))..))...((((((.((((...(((....((((....(((.(((((((((.((((((((....((((......((((((........)).))))...((((.....))))..((((....))))......))))...)))))))).))))))))).)))(((((.........)))))...........)))).............(((((((.......)))))))...))).....)))))))))).. (-141.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1141 250 201 204 155 tfor:TF3082|5end_conserved_hypothetical_protein_3316431..3316661_POSITIVE 1141 250 201 83 34 tfor:TF2202|5end_hypothetical_protein_2378682..2378912_POSITIVE 1096 250 201 224 173 tfor:TF2048|5end_conserved_hypothetical_protein_2210643..2210873_POSITIVE 1088 250 201 197 148 tfor:TF1275|5end_transcription-repair_coupling_factor_1357866..1358096_POSITIVE 1054 240 191 73 24 tfor:TF1516|5end_transposase_fragment_1626407..1626637_POSITIVE 1027 130 81 77 28 tfor:TF3003|5end_hypothetical_protein_3220382..3220612_POSITIVE 923 50 1 72 21 tfor:TF1167|5end_hypothetical_protein_1248559..1248789_POSITIVE 835 260 211 52 3 tfor:TF2668|5end_hypothetical_protein_2842471..2842701_POSITIVE 827 340 297 190 147 tfor:TF0469|5end_hypothetical_protein_486960..487190_POSITIVE 827 340 297 159 116 tfor:TF0468|5end_hypothetical_protein_486929..487159_POSITIVE 816 276 231 109 64 tfor:TF0370|5end_hypothetical_protein_386028..386258_POSITIVE 814 138 91 114 61 tfor:TF2640|5end_hypothetical_protein_2809106..2809336_POSITIVE 761 110 63 184 136 tfor:TF0499|5end_hypothetical_protein_526257..526487_POSITIVE 752 279 237 43 1 tfor:TF2665|5end_hypothetical_protein_2841973..2842203_POSITIVE 751 50 1 161 106 tfor:TF0471|5end_conserved_hypothetical_protein_487516..487746_POSITIVE 739 160 112 55 2 tfor:TF2639|5end_hypothetical_protein_2809020..2809250_POSITIVE 738 135 91 182 139 tfor:TF1274|5end_hypothetical_protein_1357621..1357851_POSITIVE 737 60 11 55 4 tfor:TF2350|5end_hypothetical_protein_2515063..2515293_POSITIVE 712 190 141 64 15 tfor:TF2025|5end_hypothetical_protein_2181933..2182163_POSITIVE 688 330 285 153 108 tfor:TF2994|5end_hypothetical_protein_3210605..3210835_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1088 250 201 146 97 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 1045 280 232 49 1 tfor:TF3003|3end_hypothetical_protein_3220629..3220779_POSITIVE 939 110 63 71 23 tfor:TF2350|3end_hypothetical_protein_2515364..2515514_POSITIVE 837 280 231 77 28 tfor:TF2172|3end_hypothetical_protein_2346135..2346285_POSITIVE 806 50 1 52 2 tfor:TF0685|3end_hypothetical_protein_731132..731282_POSITIVE 795 280 231 124 75 tfor:TF0340|3end_hypothetical_protein_364257..364407_POSITIVE 756 60 17 50 1 tfor:TF1166|3end_ABC_transporter,_permease_component_1248603..1248753_POSITIVE 751 50 1 83 28 tfor:TF0470|3end_hypothetical_protein_487518..487668_POSITIVE 739 79 31 51 2 tfor:TF1167|3end_hypothetical_protein_1248932..1249082_POSITIVE 738 135 91 45 2 tfor:TF2047|3end_hypothetical_protein_2210298..2210448_POSITIVE 736 110 63 78 32 tfor:TF0344|3end_hypothetical_protein_366963..367113_POSITIVE 734 180 131 62 13 tfor:TF0467|3end_acetyltransferase-related_protein_486543..486693_POSITIVE 718 190 142 100 52 tfor:TF0339|3end_hypothetical_protein_363747..363897_POSITIVE 706 110 61 88 42 tfor:TF0958|3end_conserved_hypothetical_protein_1004909..1005059_POSITIVE 698 350 301 119 70 tfor:TF1578|3end_hypothetical_protein_1686416..1686566_POSITIVE 689 110 61 68 17 tfor:TF2922|3end_surface_antigen_BspA_3121538..3121688_POSITIVE 685 110 61 77 23 tfor:TF1762|3end_hypothetical_protein_1899648..1899798_POSITIVE 677 350 301 63 14 tfor:TF2025|3end_hypothetical_protein_2182180..2182330_POSITIVE 622 349 301 59 11 tfor:TF2921|3end_hypothetical_protein_3118790..3118940_POSITIVE 616 110 63 80 32 tfor:TF1585|3end_hypothetical_protein_1690610..1690760_POSITIVE