Origin IGS: taaccatcgttatctttagccgtatgaacgattctatctgtcggagtaaaaacataccgatcctgaaaaatccatgtcttacattagagaaacgacaccatcctccaaaggataagtttattcatcaaacagagattgatttgcttgctccgtatttttaaaatctggtgtctcttttcagctaaaaaacctctatctttgcggtctgattcaggtttagcaaacagattacatacatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgatgtatgtaatctgtttgctaaacctgaatcagaccgcaaagatagaggttttttagctgaaaagagacaccagattttaaaaatacggagcaagcaaatcaatctctgtttgatgaataaacttatcctttggaggatggtgtcgtttctctaatgtaagacatggatttttcaggatcggtatgtttttactccgacagatagaatcgttcatacggctaaagataacgatggtta Mask Tandem Repeat Region ================================================ taaccatcgttatctttagccgtatgaacgattctatctgtcggagtaaaaacataccgatcctgaaaaatccatgtcttacattagagaaacgacaccatcctccaaaggataagtttattcatcaaacagagattgatttgcttgctccgtatttttaaaatctggtgtctcttttcagctaaaaaacctctatctttgcggtctgattcaggtttagcaaacagattacatacatca Find is-nt database================================================ Query_seq: TF0817:TF0818|TF0817:TF0818:ribonuclease, BN-like family:bifunctional protein: aspartokinase I; homoserine dehydrogenase:->->:875902..876141 240 taaccatcgttatctttagccgtatgaacgattctatctgtcggagtaaaaacataccgatcctgaaaaatccatgtcttacattagagaaacgacaccatcctccaaaggataagtttattcatcaaacagagattgatttgcttgctccgtatttttaaaatctggtgtctcttttcagctaaaaaacctctatctttgcggtctgattcaggtttagcaaacagattacatacatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0817:TF0818|TF0817:TF0818:ribonuclease, BN-like family:bifunctional protein: aspartokinase I; homoserine dehydrogenase:->->:875902..876141 240 taaccatcgttatctttagccgtatgaacgattctatctgtcggagtaaaaacataccgatcctgaaaaatccatgtcttacattagagaaacgacaccatcctccaaaggataagtttattcatcaaacagagattgatttgcttgctccgtatttttaaaatctggtgtctcttttcagctaaaaaacctctatctttgcggtctgattcaggtttagcaaacagattacatacatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0817:TF0818|TF0817:TF0818:ribonuclease, BN-like family:bifunctional protein: aspartokinase I; homoserine dehydrogenase:->->:875902..876141 240 taaccatcgttatctttagccgtatgaacgattctatctgtcggagtaaaaacataccgatcctgaaaaatccatgtcttacattagagaaacgacaccatcctccaaaggataagtttattcatcaaacagagattgatttgcttgctccgtatttttaaaatctggtgtctcttttcagctaaaaaacctctatctttgcggtctgattcaggtttagcaaacagattacatacatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaccatcgttatctttagccgtatgaacgattctatctgtcggagtaaaaacataccgatcctgaaaaatccatgtcttacattagagaaacgacaccatcctccaaaggataagtttattcatcaaacagagattgatttgcttgctccgtatttttaaaatctggtgtctcttttcagctaaaaaacctctatctttgcggtctgattcaggtttagcaaacagattacatacatca Predict ORF larger than 30AA ================================================ Protein_Len: 51 Strand: + Start: 6 End: 158 ..... M V I F S R M N D S I C R S K N I P I L K N P C L T L E K R H H P P K D K F I H Q T E I D L L A P Y F .................................................................................. Protein_Len: 47 Strand: + Start: 100 End: 240 ................................................................................................... M L Q R I S L F I K Q R L I C L L R I F K I W C L F S A K K P L S L R S D S G L A N R L H T S Protein_Len: 47 Strand: - Start: 87 End: 227 ...................................................................................... L F S V M R W L I L K N M L C L N I Q K S R I K L I Q H R K E A L F G R D K R D S E P K A F M ............. Protein_Len: 40 Strand: - Start: 2 End: 121 . V M T I K L R I F S E I Q R L L F M G I R F F G H R V N S F R C W G G F S L N M ....................................................................................................................... taaccatcgttatctttagccgtatgaacgattctatctgtcggagtaaaaacataccgatcctgaaaaatccatgtcttacattagagaaacgacaccatcctccaaaggataagtttattcatcaaacagagattgatttgcttgctccgtatttttaaaatctggtgtctcttttcagctaaaaaacctctatctttgcggtctgattcaggtttagcaaacagattacatacatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 83 Start: 135 End: 164 ......................................................................................................................................ATTGATTTGCTTGCTCCGTATTTTTAAAAT............................................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaccatcgttatctttagccgtatgaacgattctatctgtcggagtaaaaacataccgatcctgaaaaatccatgtcttacattagagaaacgacaccatcctccaaaggataagtttattcatcaaacagagattgatttgcttgctccgtatttttaaaatctggtgtctcttttcagctaaaaaacctctatctttgcggtctgattcaggtttagcaaacagattacatacatca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0817:TF0818|TF0817:TF0818:ribonuclease, BN-like family:bifunctional protein: aspartokinase I; homoserine dehydrogenase:->->:875902..876141 240 taaccatcgttatctttagccgtatgaacgattctatctgtcggagtaaaaacataccgatcctgaaaaatccatgtcttacattagagaaacgacaccatcctccaaaggataagtttattcatcaaacagagattgatttgcttgctccgtatttttaaaatctggtgtctcttttcagctaaaaaacctctatctttgcggtctgattcaggtttagcaaacagattacatacatca Intra-Species Hit: Count: 1 Min: 1 Max: 240 Len: 240 Subject: tfor_TF0817_TF0818|ribonuclease, BN-like family:bifunctional protein: aspartokinase I; homoserine dehydrogenase|POSITIVE:POSITIVE|[875902,876141]|240 HSP 1 e-value: 1.0E-133 bit: 476.0 Len: 240 Query Start:1 Query End:240 Subject Strand: POSITIVE Subject Start: 1 Subject End: 240 taaccatcgttatctttagccgtatgaacgattctatctgtcggagtaaaaacataccgatcctgaaaaatccatgtcttacattagagaaacgacaccatcctccaaaggataagtttattcatcaaacagagattgatttgcttgctccgtatttttaaaatctggtgtctcttttcagctaaaaaacctctatctttgcggtctgattcaggtttagcaaacagattacatacatca taaccatcgttatctttagccgtatgaacgattctatctgtcggagtaaaaacataccgatcctgaaaaatccatgtcttacattagagaaacgacaccatcctccaaaggataagtttattcatcaaacagagattgatttgcttgctccgtatttttaaaatctggtgtctcttttcagctaaaaaacctctatctttgcggtctgattcaggtttagcaaacagattacatacatca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACCAUCGUUAUCUUUAGCCGUAUGAACGAUUCUAUCUGUCGGAGUAAAAACAUACCGAUCCUGAAAAAUCCAUGUCUUACAUUAGAGAAACGACACCAUCCUCCAAAGGAUAAGUUUAUUCAUCAAACAGAGAUUGAUUUGCUUGCUCCGUAUUUUUAAAAUCUGGUGUCUCUUUUCAGCUAAAAAACCUCUAUCUUUGCGGUCUGAUUCAGGUUUAGCAAACAGAUUACAUACAUCA ........((((....)))).(((((...((((...((.(((((.((......)).)))))...))..))))...((((.......(((((..(((((((.........((((((((..(((.(((.......))).)))..))))).))).((....)).....)))))))..))))).(((((...((((..(((..........)))..)))))))))....))))..))))).... (-46.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUGUAUGUAAUCUGUUUGCUAAACCUGAAUCAGACCGCAAAGAUAGAGGUUUUUUAGCUGAAAAGAGACACCAGAUUUUAAAAAUACGGAGCAAGCAAAUCAAUCUCUGUUUGAUGAAUAAACUUAUCCUUUGGAGGAUGGUGUCGUUUCUCUAAUGUAAGACAUGGAUUUUUCAGGAUCGGUAUGUUUUUACUCCGACAGAUAGAAUCGUUCAUACGGCUAAAGAUAACGAUGGUUA ...((((((...((((((((.....((((((..(((((.(.......).))))).((((..((((...(((((((..(((((((...((((((.............))))))..(((((......))))).)))))))..))))))).)))).))))................)))))).((((...((....)).))))))))))))......))))))........((((....)))) (-52.82) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 313 66 21 56 6 tfor:TF3046|5end_hypothetical_protein_3274948..3275178_POSITIVE 300 42 14 51 20 tfor:TF1088|5end_transposase_fragment_1158060..1158290_POSITIVE 292 50 6 73 30 tfor:TF1455|5end_conserved_hypothetical_protein_1540688..1540918_POSITIVE 290 215 173 181 131 tfor:TF1836|5end_hypothetical_protein_1984534..1984764_POSITIVE 289 59 12 204 156 tfor:TF0252|5end_hypothetical_protein_283530..283760_POSITIVE 280 42 2 221 184 tfor:TF3068|5end_flavodoxin_A_3292812..3293042_POSITIVE 280 48 6 213 155 tfor:TF1174|5end_ABC_transporter,_permease_component_1256584..1256814_POSITIVE 276 58 13 64 13 tfor:TF1484|5end_penicillin_binding_protein_1579559..1579789_POSITIVE 274 221 190 222 178 tfor:TF1275|5end_transcription-repair_coupling_factor_1357866..1358096_POSITIVE 269 67 23 165 121 tfor:TF2315|5end_thioesterase_2473996..2474226_POSITIVE 269 238 194 114 75 tfor:TF0337|5end_hypothetical_protein_360437..360667_POSITIVE 267 105 61 152 104 tfor:TF0791|5end_transposase_845166..845396_POSITIVE 265 48 4 67 27 tfor:TF2583|5end_possible_ABC_transporter,__permease_component_2750206..2750436_POSITIVE 264 208 164 169 130 tfor:TF1714|5end_hypothetical_protein_1834125..1834355_POSITIVE 263 183 141 116 70 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 263 183 141 64 18 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 262 42 18 225 200 tfor:TF3150|5end_conserved_hypothetical_protein_3385512..3385742_POSITIVE 262 219 194 231 205 tfor:TF1203|5end_L-asparaginase_I_1285232..1285462_POSITIVE 261 63 21 89 42 tfor:TF0645|5end_hypothetical_protein_688673..688903_POSITIVE 260 64 21 166 122 tfor:TF1439|5end_outer_membrane_protein,_TonB_dependent_receptor_1522479..1522709_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 327 237 194 90 48 tfor:TF1202|3end_antitermination_factor,_NusB_family_1285155..1285305_POSITIVE 300 42 14 136 105 tfor:TF1087|3end_ISPg4_transposase_1158225..1158375_POSITIVE 289 59 12 123 75 tfor:TF0251|3end_conserved_hypothetical_protein;_possible_exporter_283529..283679_POSITIVE 286 206 173 141 103 tfor:TF1835|3end_possible_dihydroxy-acid_dehydratase_1984586..1984736_POSITIVE 284 239 193 141 100 tfor:TF0535|3end_surface_antigen_BspA_561701..561851_POSITIVE 281 60 18 119 65 tfor:TF0686|3end_hypothetical_protein_732950..733100_POSITIVE 274 208 169 87 44 tfor:TF0684|3end_conserved_hypothetical_protein_730564..730714_POSITIVE 269 67 23 133 89 tfor:TF2314|3end_conserved_hypothetical_protein_2474044..2474194_POSITIVE 269 238 194 55 16 tfor:TF0334|3end_hypothetical_protein_360458..360608_POSITIVE 265 48 4 64 24 tfor:TF2582|3end_ABC_transporter,_ATP-binding_protein_2750283..2750433_POSITIVE 265 178 131 112 55 tfor:TF1914|3end_conserved_hypothetical_protein_2067656..2067806_POSITIVE 263 207 166 99 53 tfor:TF2196|3end_hypothetical_protein_2372180..2372330_POSITIVE 262 46 7 105 70 tfor:TF0279|3end_conserved_hypothetical_protein_308679..308829_POSITIVE 260 64 21 131 87 tfor:TF1438|3end_hypothetical_protein_1522524..1522674_POSITIVE 259 49 4 133 79 tfor:TF2388|3end_outer_membrane_protein,_TonB_dependent_receptor_2558905..2559055_POSITIVE 259 49 2 60 7 tfor:TF0328|3end_conserved_hypothetical_protein_355266..355416_POSITIVE 258 227 181 105 64 tfor:TF3165|3end_thiol:disulfide_interchange_protein,_thioredoxin_family_protein_3405343..3405493_POSITIVE 258 229 189 85 48 tfor:TF2280|3end_hypothetical_protein_2439474..2439624_POSITIVE 256 218 172 62 13 tfor:TF2223|3end_CTn_excision__protein_2394632..2394782_POSITIVE 256 180 131 141 96 tfor:TF2167|3end_hypothetical_protein_2343979..2344129_POSITIVE