Origin IGS: taaacataacacagtgcatttttctgccgaagaattatgttggatagcataaaacgccttatctttgcatcgtgtttttcatggtattagatttaaggttaacaatgaagattgtggttgtcgtgagacaaccatttcttttttatagaagttcctaaatttcacctcccttttccctctatctaactaaaacaagaattaattccgtttttgtcattaactaatgaaaaaacgctatatttgcaccctaaaaaaaagaggaataagaacaaataacatataaatcaagaaattcaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttgaatttcttgatttatatgttatttgttcttattcctcttttttttagggtgcaaatatagcgttttttcattagttaatgacaaaaacggaattaattcttgttttagttagatagagggaaaagggaggtgaaatttaggaacttctataaaaaagaaatggttgtctcacgacaaccacaatcttcattgttaaccttaaatctaataccatgaaaaacacgatgcaaagataaggcgttttatgctatccaacataattcttcggcagaaaaatgcactgtgttatgttta Mask Tandem Repeat Region ================================================ taaacataacacagtgcatttttctgccgaagaattatgttggatagcataaaacgccttatctttgcatcgtgtttttcatggtattagatttaaggttaacaatgaagattgtggttgtcgtgagacaaccatttcttttttatagaagttcctaaatttcacctcccttttccctctatctaactaaaacaagaattaattccgtttttgtcattaactaatgaaaaaacgctatatttgcaccctaaaaaaaagaggaataagaacaaataacatataaatcaagaaattcaa Find is-nt database================================================ Query_seq: TF0787:TF0788|TF0787:TF0788:possible inorganic polyphosphate; ATP-NAD kinase:protein-export membrane protein:->->:840405..840701 297 taaacataacacagtgcatttttctgccgaagaattatgttggatagcataaaacgccttatctttgcatcgtgtttttcatggtattagatttaaggttaacaatgaagattgtggttgtcgtgagacaaccatttcttttttatagaagttcctaaatttcacctcccttttccctctatctaactaaaacaagaattaattccgtttttgtcattaactaatgaaaaaacgctatatttgcaccctaaaaaaaagaggaataagaacaaataacatataaatcaagaaattcaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0787:TF0788|TF0787:TF0788:possible inorganic polyphosphate; ATP-NAD kinase:protein-export membrane protein:->->:840405..840701 297 taaacataacacagtgcatttttctgccgaagaattatgttggatagcataaaacgccttatctttgcatcgtgtttttcatggtattagatttaaggttaacaatgaagattgtggttgtcgtgagacaaccatttcttttttatagaagttcctaaatttcacctcccttttccctctatctaactaaaacaagaattaattccgtttttgtcattaactaatgaaaaaacgctatatttgcaccctaaaaaaaagaggaataagaacaaataacatataaatcaagaaattcaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0787:TF0788|TF0787:TF0788:possible inorganic polyphosphate; ATP-NAD kinase:protein-export membrane protein:->->:840405..840701 297 taaacataacacagtgcatttttctgccgaagaattatgttggatagcataaaacgccttatctttgcatcgtgtttttcatggtattagatttaaggttaacaatgaagattgtggttgtcgtgagacaaccatttcttttttatagaagttcctaaatttcacctcccttttccctctatctaactaaaacaagaattaattccgtttttgtcattaactaatgaaaaaacgctatatttgcaccctaaaaaaaagaggaataagaacaaataacatataaatcaagaaattcaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaacataacacagtgcatttttctgccgaagaattatgttggatagcataaaacgccttatctttgcatcgtgtttttcatggtattagatttaaggttaacaatgaagattgtggttgtcgtgagacaaccatttcttttttatagaagttcctaaatttcacctcccttttccctctatctaactaaaacaagaattaattccgtttttgtcattaactaatgaaaaaacgctatatttgcaccctaaaaaaaagaggaataagaacaaataacatataaatcaagaaattcaa Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: - Start: 186 End: 281 ......................................................................................................................................................................................... S F C S N I G N K D N V L S F V S Y K C G L F F L F L F L Y C M ................ taaacataacacagtgcatttttctgccgaagaattatgttggatagcataaaacgccttatctttgcatcgtgtttttcatggtattagatttaaggttaacaatgaagattgtggttgtcgtgagacaaccatttcttttttatagaagttcctaaatttcacctcccttttccctctatctaactaaaacaagaattaattccgtttttgtcattaactaatgaaaaaacgctatatttgcaccctaaaaaaaagaggaataagaacaaataacatataaatcaagaaattcaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaacataacacagtgcatttttctgccgaagaattatgttggatagcataaaacgccttatctttgcatcgtgtttttcatggtattagatttaaggttaacaatgaagattgtggttgtcgtgagacaaccatttcttttttatagaagttcctaaatttcacctcccttttccctctatctaactaaaacaagaattaattccgtttttgtcattaactaatgaaaaaacgctatatttgcaccctaaaaaaaagaggaataagaacaaataacatataaatcaagaaattcaa Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 71 HP_score: -3.2 Tail_Score: -5.25803 Start: 110 End: 138 Full_Region: AAGGTTAACAATGAA GATTGTGGTTGTC GTGA GACAACCATTTC TTTTTTATAGAAGTT .............................................................................................................GATTGTGGTTGTCGTGAGACAACCATTTC............................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -10.8 Tail_Score: -5.13354 Start: 115 End: 134 Full_Region: TAACAATGAAGATTG TGGTTGTC GTGA GACAACCA TTTCTTTTTTATAGA ..................................................................................................................TGGTTGTCGTGAGACAACCA................................................................................................................................................................... Find igs database================================================ Query_seq: TF0787:TF0788|TF0787:TF0788:possible inorganic polyphosphate; ATP-NAD kinase:protein-export membrane protein:->->:840405..840701 297 taaacataacacagtgcatttttctgccgaagaattatgttggatagcataaaacgccttatctttgcatcgtgtttttcatggtattagatttaaggttaacaatgaagattgtggttgtcgtgagacaaccatttcttttttatagaagttcctaaatttcacctcccttttccctctatctaactaaaacaagaattaattccgtttttgtcattaactaatgaaaaaacgctatatttgcaccctaaaaaaaagaggaataagaacaaataacatataaatcaagaaattcaa Intra-Species Hit: Count: 1 Min: 1 Max: 297 Len: 297 Subject: tfor_TF0787_TF0788|possible inorganic polyphosphate; ATP-NAD kinase:protein-export membrane protein|POSITIVE:POSITIVE|[840405,840701]|297 HSP 1 e-value: 1.0E-153 bit: 541.0 Len: 297 Query Start:1 Query End:297 Subject Strand: POSITIVE Subject Start: 1 Subject End: 297 taaacataacacagtgcatttttctgccgaagaattatgttggatagcataaaacgccttatctttgcatcgtgtttttcatggtattagatttaaggttaacaatgaagattgtggttgtcgtgagacaaccatttcttttttatagaagttcctaaatttcacctcccttttccctctatctaactaaaacaagaattaattccgtttttgtcattaactaatgaaaaaacgctatatttgcaccctnnnnnnnngaggaataagaacaaataacatataaatcaagaaattcaa taaacataacacagtgcatttttctgccgaagaattatgttggatagcataaaacgccttatctttgcatcgtgtttttcatggtattagatttaaggttaacaatgaagattgtggttgtcgtgagacaaccatttcttttttatagaagttcctaaatttcacctcccttttccctctatctaactaaaacaagaattaattccgtttttgtcattaactaatgaaaaaacgctatatttgcaccctaaaaaaaagaggaataagaacaaataacatataaatcaagaaattcaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACAUAACACAGUGCAUUUUUCUGCCGAAGAAUUAUGUUGGAUAGCAUAAAACGCCUUAUCUUUGCAUCGUGUUUUUCAUGGUAUUAGAUUUAAGGUUAACAAUGAAGAUUGUGGUUGUCGUGAGACAACCAUUUCUUUUUUAUAGAAGUUCCUAAAUUUCACCUCCCUUUUCCCUCUAUCUAACUAAAACAAGAAUUAAUUCCGUUUUUGUCAUUAACUAAUGAAAAAACGCUAUAUUUGCACCCUAAAAAAAAGAGGAAUAAGAACAAAUAACAUAUAAAUCAAGAAAUUCAA ....((((((((.(((((.(((((....))))).((((((......)))))).............))))).)))))....)))......((((((..((((.....(((((..(((((((((....))))))))).)))))......((((((....))))))......(((...(((((......((......)).........(((((((.(((((....))))))))))))......................)))))...)))......))))...))))))........... (-59.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGAAUUUCUUGAUUUAUAUGUUAUUUGUUCUUAUUCCUCUUUUUUUUAGGGUGCAAAUAUAGCGUUUUUUCAUUAGUUAAUGACAAAAACGGAAUUAAUUCUUGUUUUAGUUAGAUAGAGGGAAAAGGGAGGUGAAAUUUAGGAACUUCUAUAAAAAAGAAAUGGUUGUCUCACGACAACCACAAUCUUCAUUGUUAACCUUAAAUCUAAUACCAUGAAAAACACGAUGCAAAGAUAAGGCGUUUUAUGCUAUCCAACAUAAUUCUUCGGCAGAAAAAUGCACUGUGUUAUGUUUA ...........((((((...((((...(((((((..((((((((((((...............((((((((((((....))))).))))))).((((((........))))))........)))))))))))).......)))))))....((((..((((..((((((((....))))))))...))))..))))))))..))))))............(((((..(((((........(((((((.((((.................))))..)))))))..)))))..))))). (-60.53) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 317 60 14 108 68 tfor:TF0600|5end_oxidoreductase/dehydrogenase_630083..630313_POSITIVE 299 88 47 66 19 tfor:TF2896|5end_conserved_hypothetical_protein;_possible_ATPase,_AAA_superfamily_3084393..3084623_POSITIVE 278 183 145 187 146 tfor:TF2636|5end_glutaminase_2806096..2806326_POSITIVE 274 278 234 228 185 tfor:TF1621|5end_DNA_replication_and_repair_protein_RecF_1735293..1735523_POSITIVE 270 86 44 58 13 tfor:TF2218|5end_two-component_system_response_regulator_2390162..2390392_POSITIVE 270 69 24 46 1 tfor:TF1418|5end_cell_division_protein_1500027..1500257_POSITIVE 266 74 38 43 2 tfor:TF3022|5end_chorismate_synthase_3246136..3246366_POSITIVE 265 186 163 129 105 tfor:TF0340|5end_hypothetical_protein_363893..364123_POSITIVE 264 124 98 27 1 tfor:TF3073|5end_magnesium-protoporphyrin_O-methyltransferase;_cobalamin_biosynthesis_protein_N_3296008..3296238_POSITIVE 261 128 81 143 97 tfor:TF2010|5end_hypothetical_protein_2163246..2163476_POSITIVE 260 70 22 180 127 tfor:TF2012|5end_conserved_hypothetical_protein_2165380..2165610_POSITIVE 260 110 62 69 2 tfor:TF1250|5end_possible_cation_efflux_pump_1332324..1332554_POSITIVE 259 80 34 192 152 tfor:TF2777|5end_hypothetical_protein_2961595..2961825_POSITIVE 259 80 34 67 27 tfor:TF2776|5end_hypothetical_protein_2961470..2961700_POSITIVE 259 80 36 152 97 tfor:TF2597|5end_outer_membrane_receptor_protein;_possible_TonB_dependent_receptor_2767916..2768146_POSITIVE 259 68 29 228 196 tfor:TF0396|5end_conserved_hypothetical_protein_417018..417248_POSITIVE 252 133 97 100 58 tfor:TF2045|5end_aminoacyl-histidine_dipeptidase_2206114..2206344_POSITIVE 252 79 32 80 31 tfor:TF2042|5end_sugar_phosphate_isomerase,_polysialic_acid_capsule_expression_protein_2201647..2201877_POSITIVE 251 99 52 182 141 tfor:TF3003|5end_hypothetical_protein_3220382..3220612_POSITIVE 251 90 42 223 183 tfor:TF1713|5end_hypothetical_protein_1833442..1833672_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 484 128 81 61 13 tfor:TF1810|3end_conserved_hypothetical_protein;_possible_membrane_protein_1952306..1952456_POSITIVE 317 60 14 75 35 tfor:TF0599|3end_hypothetical_protein_630130..630280_POSITIVE 278 183 145 112 71 tfor:TF2635|3end_ABC_transporter,_ATP-binding_protein/permease_component_2806101..2806251_POSITIVE 275 100 51 90 47 tfor:TF1500|3end_conserved_hypothetical_protein_1600354..1600504_POSITIVE 274 278 234 78 35 tfor:TF1620|3end_TPR_domain_protein_1735223..1735373_POSITIVE 262 68 21 99 45 tfor:TF3013|3end_conserved_hypothetical_protein_3237539..3237689_POSITIVE 261 128 81 62 16 tfor:TF2009|3end_octaprenyl-diphosphate_synthase_ispB_2163245..2163395_POSITIVE 259 80 36 135 80 tfor:TF2596|3end_conserved_hypothetical_protein;_possible_lipoprotein_2767979..2768129_POSITIVE 259 74 36 58 18 tfor:TF1903|3end_hypothetical_protein_2057627..2057777_POSITIVE 252 124 102 22 1 tfor:TF3072|3end_conserved_hypothetical_protein_3296083..3296233_POSITIVE 252 133 97 69 27 tfor:TF2044|3end_excinuclease_ABC,_subunit_A_2206163..2206313_POSITIVE 249 68 22 69 25 tfor:TF0434|3end_CTP_synthetase_(UTP_ammonia_ligase)_457027..457177_POSITIVE 246 72 55 20 3 tfor:TF3043|3end_conserved_hypothetical_protein_3272409..3272559_POSITIVE 244 80 46 69 36 tfor:TF2592|3end_conserved_hypothetical_protein_2762807..2762957_POSITIVE 244 270 224 96 45 tfor:TF0325|3end_LysM_domain_protein_353784..353934_POSITIVE 243 59 12 52 8 tfor:TF2246|3end_hypothetical_protein_2421550..2421700_POSITIVE 243 76 42 125 98 tfor:TF1292|3end_sensor_histidine_protein_kinase_1374554..1374704_POSITIVE 242 60 24 61 24 tfor:TF2853|3end_2-C-methyl-D-erythritol_2,4-cyclodiphosphate_synthase_3040725..3040875_POSITIVE 242 147 103 77 26 tfor:TF0246|3end_saccharopine_dehydrogenase_277530..277680_POSITIVE 241 186 163 25 3 tfor:TF2171|3end_hypothetical_protein_2345792..2345942_POSITIVE