Origin IGS: gcaagatcagtcttatttcgttcacttactctatatttgtcgagaaatatacatgcaaatgtaggatgaattgagaaattattaaagaaattcggtgcaaaagatttataaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttataaatcttttgcaccgaatttctttaataatttctcaattcatcctacatttgcatgtatatttctcgacaaatatagagtaagtgaacgaaataagactgatcttgc Mask Tandem Repeat Region ================================================ gcaagatcagtcttatttcgttcacttactctatatttgtcgagaaatatacatgcaaatgtaggatgaattgagaaattattaaagaaattcggtgcaaaagatttataaa Find is-nt database================================================ Query_seq: TF0786:TF0787|TF0786:TF0787:pyridoxal phosphate biosynthetic protein:possible inorganic polyphosphate; ATP-NAD kinase:->->:839420..839531 112 gcaagatcagtcttatttcgttcacttactctatatttgtcgagaaatatacatgcaaatgtaggatgaattgagaaattattaaagaaattcggtgcaaaagatttataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0786:TF0787|TF0786:TF0787:pyridoxal phosphate biosynthetic protein:possible inorganic polyphosphate; ATP-NAD kinase:->->:839420..839531 112 gcaagatcagtcttatttcgttcacttactctatatttgtcgagaaatatacatgcaaatgtaggatgaattgagaaattattaaagaaattcggtgcaaaagatttataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0786:TF0787|TF0786:TF0787:pyridoxal phosphate biosynthetic protein:possible inorganic polyphosphate; ATP-NAD kinase:->->:839420..839531 112 gcaagatcagtcttatttcgttcacttactctatatttgtcgagaaatatacatgcaaatgtaggatgaattgagaaattattaaagaaattcggtgcaaaagatttataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gcaagatcagtcttatttcgttcacttactctatatttgtcgagaaatatacatgcaaatgtaggatgaattgagaaattattaaagaaattcggtgcaaaagatttataaa Predict ORF larger than 30AA ================================================ gcaagatcagtcttatttcgttcacttactctatatttgtcgagaaatatacatgcaaatgtaggatgaattgagaaattattaaagaaattcggtgcaaaagatttataaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gcaagatcagtcttatttcgttcacttactctatatttgtcgagaaatatacatgcaaatgtaggatgaattgagaaattattaaagaaattcggtgcaaaagatttataaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0786:TF0787|TF0786:TF0787:pyridoxal phosphate biosynthetic protein:possible inorganic polyphosphate; ATP-NAD kinase:->->:839420..839531 112 gcaagatcagtcttatttcgttcacttactctatatttgtcgagaaatatacatgcaaatgtaggatgaattgagaaattattaaagaaattcggtgcaaaagatttataaa Intra-Species Hit: Count: 1 Min: 1 Max: 112 Len: 112 Subject: tfor_TF0786_TF0787|pyridoxal phosphate biosynthetic protein:possible inorganic polyphosphate; ATP-NAD kinase|POSITIVE:POSITIVE|[839420,839531]|112 HSP 1 e-value: 2.0E-57 bit: 222.0 Len: 112 Query Start:1 Query End:112 Subject Strand: POSITIVE Subject Start: 1 Subject End: 112 gcaagatcagtcttatttcgttcacttactctatatttgtcgagaaatatacatgcaaatgtaggatgaattgagaaattattaaagaaattcggtgcaaaagatttataaa gcaagatcagtcttatttcgttcacttactctatatttgtcgagaaatatacatgcaaatgtaggatgaattgagaaattattaaagaaattcggtgcaaaagatttataaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCAAGAUCAGUCUUAUUUCGUUCACUUACUCUAUAUUUGUCGAGAAAUAUACAUGCAAAUGUAGGAUGAAUUGAGAAAUUAUUAAAGAAAUUCGGUGCAAAAGAUUUAUAAA ...(((((..(((((.(((((((.....(((.((....)).))).....(((((....)))))))))))).)))))...(((..((....))..))).....)))))..... (-20.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUAUAAAUCUUUUGCACCGAAUUUCUUUAAUAAUUUCUCAAUUCAUCCUACAUUUGCAUGUAUAUUUCUCGACAAAUAUAGAGUAAGUGAACGAAAUAAGACUGAUCUUGC ........((((...(...(((((................)))))......(((((((.(.(((((((......))))))).))))))))...)....)))).......... (-11.99) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 247 99 55 150 109 tfor:TF1554|5end_UDP-glucose_dehydrogenase_1659623..1659853_POSITIVE 239 43 1 144 97 tfor:TF2218|5end_two-component_system_response_regulator_2390162..2390392_POSITIVE 237 42 5 47 1 tfor:TF1244|5end_hypothetical_protein_1328554..1328784_POSITIVE 223 41 1 127 87 tfor:TF1579|5end_hypothetical_protein_1686889..1687119_POSITIVE 220 49 16 42 1 tfor:TF0176|5end_hypothetical_protein_186520..186750_POSITIVE 218 98 58 105 57 tfor:TF1859|5end_aspartate_carbamoyltransferase,_regulatory_subunit_2015711..2015941_POSITIVE 216 97 53 137 96 tfor:TF0880|5end_conserved_hypothetical_protein_934010..934240_POSITIVE 214 45 1 167 127 tfor:TF2100|5end_hypothetical_protein_2273424..2273654_POSITIVE 212 43 3 202 164 tfor:TF2315|5end_thioesterase_2473996..2474226_POSITIVE 208 98 53 87 45 tfor:TF2887|5end_phosphomannomutase/phosphoglucomutase_3076327..3076557_POSITIVE 208 76 37 147 108 tfor:TF0319|5end_tolB_protein_344539..344769_POSITIVE 207 97 51 183 137 tfor:TF2508|5end_ATP-dependent_Clp_protease,_ATP-binding_subunit_clpX_2686315..2686545_POSITIVE 207 100 54 172 126 tfor:TF1897|5end_conserved_hypothetical_protein;_possible_aminopeptidase_2049259..2049489_POSITIVE 206 67 29 223 186 tfor:TF0937|5end_ATPase,__AAA+_class_978390..978620_POSITIVE 205 42 1 211 172 tfor:TF2610|5end_nitroreductase_2786376..2786606_POSITIVE 205 100 58 132 93 tfor:TF1434|5end_transketolase_1517485..1517715_POSITIVE 204 49 1 55 16 tfor:TF1462|5end_erythronate-4-phosphate_dehydrogenase_1552184..1552414_POSITIVE 202 76 40 40 6 tfor:TF2175|5end_hypothetical_protein_2348535..2348765_POSITIVE 201 31 3 52 21 tfor:TF3100|5end_conserved_hypothetical_protein_3331185..3331415_POSITIVE 201 43 2 61 14 tfor:TF2943|5end_50S_ribosomal_protein_L7/L12_3143116..3143346_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 247 99 55 128 87 tfor:TF1553|3end_copper_homeostasis_protein_1659681..1659831_POSITIVE 240 74 36 146 103 tfor:TF0070|3end_possible_regulatory_element_77735..77885_POSITIVE 239 43 1 67 20 tfor:TF2217|3end_hypothetical_protein_2390165..2390315_POSITIVE 218 98 58 86 38 tfor:TF1858|3end_possible_flavoredoxin_2015772..2015922_POSITIVE 216 97 53 132 91 tfor:TF0879|3end_TraG_family_protein_934085..934235_POSITIVE 214 45 1 82 42 tfor:TF2099|3end_D-tyrosyl-tRNA(Tyr)_deacylase_2273419..2273569_POSITIVE 211 44 1 115 62 tfor:TF2889|3end_50S_ribosomal_protein_L27_3078688..3078838_POSITIVE 205 100 58 118 79 tfor:TF1433|3end_ribose_5-phosphate_isomerase_1517551..1517701_POSITIVE 204 49 1 44 5 tfor:TF1461|3end_DNA-damage-inducible_protein_F_1552253..1552403_POSITIVE 202 76 40 40 6 tfor:TF2174|3end_possible_endopeptidase_precursor_2348615..2348765_POSITIVE 201 50 1 61 17 tfor:TF1159|3end_hypothetical_protein_1240342..1240492_POSITIVE 201 74 51 102 75 tfor:TF0608|3end_isoleucyl-tRNA_synthetase_641418..641568_POSITIVE 200 43 1 107 51 tfor:TF2604|3end_possible_membrane_transport_protein_2779832..2779982_POSITIVE 199 100 60 57 11 tfor:TF0691|3end_conserved_hypothetical_protein_736452..736602_POSITIVE 197 66 37 145 117 tfor:TF0379|3end_conserved_hypothetical_protein,_nif3_family_400632..400782_POSITIVE 197 43 9 128 84 tfor:TF0253|3end_conserved_hypothetical_protein_285340..285490_POSITIVE 195 43 6 111 71 tfor:TF2569|3end_50S_ribosomal_protein_L6_2742250..2742400_POSITIVE 194 100 57 105 70 tfor:TF2731|3end_Fe2+/Zn2+_uptake_regulation_protein_2911686..2911836_POSITIVE 194 59 18 107 60 tfor:TF2288|3end_hypothetical_protein_2442598..2442748_POSITIVE 194 48 2 52 6 tfor:TF2223|3end_CTn_excision__protein_2394632..2394782_POSITIVE