Origin IGS: taatgttcctcgacctcctctctctcgatagagaatggaacgttatctcagcctttgttgcaatccgtttgcctcgtttcttatcggtaagattccggcctttgtgccgggattgataagaaacgaggcaataagtttagggctgttca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaacagccctaaacttattgcctcgtttcttatcaatcccggcacaaaggccggaatcttaccgataagaaacgaggcaaacggattgcaacaaaggctgagataacgttccattctctatcgagagagaggaggtcgaggaacatta Mask Tandem Repeat Region ================================================ taatgttcctcgacctcctctctctcgatagagaatggaacgttatctcagcctttgttgcaatccgtttgcctcgtttcttatcggtaagattccggcctttgtgccgggattgataagaaacgaggcaataagtttagggctgttca Find is-nt database================================================ Query_seq: TF0779:TF0780|TF0779:TF0780:possible outer membrane protein:alpha-L-arabinofuranosidase, precursor A:->->:831935..832083 149 taatgttcctcgacctcctctctctcgatagagaatggaacgttatctcagcctttgttgcaatccgtttgcctcgtttcttatcggtaagattccggcctttgtgccgggattgataagaaacgaggcaataagtttagggctgttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0779:TF0780|TF0779:TF0780:possible outer membrane protein:alpha-L-arabinofuranosidase, precursor A:->->:831935..832083 149 taatgttcctcgacctcctctctctcgatagagaatggaacgttatctcagcctttgttgcaatccgtttgcctcgtttcttatcggtaagattccggcctttgtgccgggattgataagaaacgaggcaataagtttagggctgttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0779:TF0780|TF0779:TF0780:possible outer membrane protein:alpha-L-arabinofuranosidase, precursor A:->->:831935..832083 149 taatgttcctcgacctcctctctctcgatagagaatggaacgttatctcagcctttgttgcaatccgtttgcctcgtttcttatcggtaagattccggcctttgtgccgggattgataagaaacgaggcaataagtttagggctgttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatgttcctcgacctcctctctctcgatagagaatggaacgttatctcagcctttgttgcaatccgtttgcctcgtttcttatcggtaagattccggcctttgtgccgggattgataagaaacgaggcaataagtttagggctgttca Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 3 End: 113 .. M F L D L L S L D R E W N V I S A F V A I R L P R F L S V R F R P L C R D .................................... Protein_Len: 38 Strand: + Start: 35 End: 148 .................................. M E R Y L S L C C N P F A S F L I G K I P A F V P G L I R N E A I S L G L F . taatgttcctcgacctcctctctctcgatagagaatggaacgttatctcagcctttgttgcaatccgtttgcctcgtttcttatcggtaagattccggcctttgtgccgggattgataagaaacgaggcaataagtttagggctgttca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatgttcctcgacctcctctctctcgatagagaatggaacgttatctcagcctttgttgcaatccgtttgcctcgtttcttatcggtaagattccggcctttgtgccgggattgataagaaacgaggcaataagtttagggctgttca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0779:TF0780|TF0779:TF0780:possible outer membrane protein:alpha-L-arabinofuranosidase, precursor A:->->:831935..832083 149 taatgttcctcgacctcctctctctcgatagagaatggaacgttatctcagcctttgttgcaatccgtttgcctcgtttcttatcggtaagattccggcctttgtgccgggattgataagaaacgaggcaataagtttagggctgttca Intra-Species Hit: Count: 1 Min: 1 Max: 149 Len: 149 Subject: tfor_TF0779_TF0780|possible outer membrane protein:alpha-L-arabinofuranosidase, precursor A|POSITIVE:POSITIVE|[831935,832083]|149 HSP 1 e-value: 2.0E-79 bit: 295.0 Len: 149 Query Start:1 Query End:149 Subject Strand: POSITIVE Subject Start: 1 Subject End: 149 taatgttcctcgacctcctctctctcgatagagaatggaacgttatctcagcctttgttgcaatccgtttgcctcgtttcttatcggtaagattccggcctttgtgccgggattgataagaaacgaggcaataagtttagggctgttca taatgttcctcgacctcctctctctcgatagagaatggaacgttatctcagcctttgttgcaatccgtttgcctcgtttcttatcggtaagattccggcctttgtgccgggattgataagaaacgaggcaataagtttagggctgttca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGUUCCUCGACCUCCUCUCUCUCGAUAGAGAAUGGAACGUUAUCUCAGCCUUUGUUGCAAUCCGUUUGCCUCGUUUCUUAUCGGUAAGAUUCCGGCCUUUGUGCCGGGAUUGAUAAGAAACGAGGCAAUAAGUUUAGGGCUGUUCA (((((((((..((.....))..((((....))))..)))))))))...(((((((....((.......(((((((((((((((((.....(((((((((......))))))))))))))))))))))))))...))..))))))).... (-61.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAACAGCCCUAAACUUAUUGCCUCGUUUCUUAUCAAUCCCGGCACAAAGGCCGGAAUCUUACCGAUAAGAAACGAGGCAAACGGAUUGCAACAAAGGCUGAGAUAACGUUCCAUUCUCUAUCGAGAGAGAGGAGGUCGAGGAACAUUA ....(((((.........(((((((((((((((((....(((((......))))).........)))))))))))))))))..(........)...))))).......(((((..(((((.((....))..)))))....))))).... (-56.62) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 389 109 66 110 61 tfor:TF0261|5end_conserved_hypothetical_protein_291784..292014_POSITIVE 371 100 58 201 153 tfor:TF2025|5end_hypothetical_protein_2181933..2182163_POSITIVE 371 130 95 131 84 tfor:TF1049|5end_cobyrinic_acid_a,c-diamide_synthase_1110140..1110370_POSITIVE 370 88 49 36 1 tfor:TF1962|5end_TatD-related_deoxyribonuclease,_Mg-dependent_DNase_2112417..2112647_POSITIVE 370 109 67 59 24 tfor:TF0414|5end_N-ethylammeline_chlorohydrolase_431789..432019_POSITIVE 364 110 63 192 143 tfor:TF2742|5end_single-strand_DNA-specific_exonuclease_2923802..2924032_POSITIVE 362 110 69 156 114 tfor:TF0236|5end_multidrug_resistance_protein_261779..262009_POSITIVE 357 99 51 210 150 tfor:TF0463|5end_precorrin-6Y_C5,15-methyltransferase_479015..479245_POSITIVE 356 110 64 194 151 tfor:TF2683|5end_translation_initiation_factor_IF-2_2857722..2857952_POSITIVE 355 110 68 218 185 tfor:TF0972|5end_conserved_hypothetical_protein_1022798..1023028_POSITIVE 353 112 71 74 28 tfor:TF0815|5end_conserved_hypothetical_protein_873199..873429_POSITIVE 352 107 64 226 190 tfor:TF2804|5end_conserved_hypothetical_protein_2993494..2993724_POSITIVE 351 99 51 156 101 tfor:TF0992|5end_hypothetical_protein_1048251..1048481_POSITIVE 350 110 66 214 170 tfor:TF2596|5end_conserved_hypothetical_protein;_possible_lipoprotein_2766667..2766897_POSITIVE 350 69 23 199 147 tfor:TF1598|5end_conserved_hypothetical_protein_1713358..1713588_POSITIVE 347 110 65 218 161 tfor:TF0917|5end_DNA-damage-inducible_protein;_possible_SOS_response_protein_956235..956465_POSITIVE 344 118 71 204 147 tfor:TF2506|5end_possible_peptidyl-prolyl_cis-trans_isomerase_2682292..2682522_POSITIVE 344 110 63 226 181 tfor:TF1315|5end_purine_nucleoside_phosphorylase_1390236..1390466_POSITIVE 343 100 55 187 149 tfor:TF1472|5end_acyl-ACP_thioesterase_1565475..1565705_POSITIVE 343 110 64 73 19 tfor:TF0525|5end_conserved_hypothetical_protein_549265..549495_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 371 130 95 128 81 tfor:TF1048|3end_permease_1110217..1110367_POSITIVE 370 109 67 59 24 tfor:TF0413|3end_succinyl-diaminopimelate_desuccinylase_431869..432019_POSITIVE 368 108 61 147 94 tfor:TF2515|3end_outer_membrane_protein,_TonB_dependent_receptor_2694865..2695015_POSITIVE 363 108 66 119 66 tfor:TF0886|3end_conjugative_transposon_protein_fragment_938152..938302_POSITIVE 362 110 69 146 104 tfor:TF0235|3end_hypothetical_protein_261849..261999_POSITIVE 358 109 64 125 83 tfor:TF2803|3end_dehydrogenase,_possible_NADH-dependent_dehydrogenase_2993467..2993617_POSITIVE 343 110 64 60 6 tfor:TF0524|3end_possible_cation_efflux_pump_(multidrug_resistance_protein)_549332..549482_POSITIVE 342 115 71 151 110 tfor:TF0282|3end_probable_tonB-linked_outer_membrane_receptor_311376..311526_POSITIVE 341 107 71 151 116 tfor:TF0308|3end_hypothetical_protein_334082..334232_POSITIVE 339 120 72 150 102 tfor:TF2252|3end_transfer_region-related_protein,_TraF_2425030..2425180_POSITIVE 338 117 71 73 16 tfor:TF2770|3end_conserved_hypothetical_protein_2956639..2956789_POSITIVE 330 110 64 57 12 tfor:TF1298|3end_possible_membrane_protein_1381197..1381347_POSITIVE 325 110 65 148 102 tfor:TF2710|3end_ABC_transporter,_ATP-binding_protein/permease_component_2884615..2884765_POSITIVE 321 109 63 96 49 tfor:TF0629|3end_hypothetical_protein_666710..666860_POSITIVE 321 109 64 65 25 tfor:TF0490|3end_conserved_hypothetical_protein_513904..514054_POSITIVE 318 98 63 111 74 tfor:TF3133|3end_conserved_hypothetical_protein_3369913..3370063_POSITIVE 318 110 64 54 24 tfor:TF1917|3end_hypothetical_protein_2070244..2070394_POSITIVE 318 100 53 151 91 tfor:TF1191|3end_possible_transcriptional_regulator_1273979..1274129_POSITIVE 317 100 54 67 8 tfor:TF1431|3end_conserved_hypothetical_protein;_possible_acyltransferase_1516008..1516158_POSITIVE 315 130 81 136 93 tfor:TF2946|3end_conserved_hypothetical_protein;_possible_ATPase_3153362..3153512_POSITIVE