Origin IGS: tattatctataatccgtttatttgttggattttgaggcacaaaggtagataattttcccggatatcgttttttttatgtgtaaaatttgcatgattataccttaattatatttactttcgcat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atgcgaaagtaaatataattaaggtataatcatgcaaattttacacataaaaaaaacgatatccgggaaaattatctacctttgtgcctcaaaatccaacaaataaacggattatagataata Mask Tandem Repeat Region ================================================ tattatctataatccgtttatttgttggattttgaggcacaaaggtagataattttcccggatatcgttttttttatgtgtaaaatttgcatgattataccttaattatatttactttcgcat Find is-nt database================================================ Query_seq: TF0761:TF0762|TF0761:TF0762:conserved hypothetical protein:chromate transport protein:->->:809141..809263 123 tattatctataatccgtttatttgttggattttgaggcacaaaggtagataattttcccggatatcgttttttttatgtgtaaaatttgcatgattataccttaattatatttactttcgcat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0761:TF0762|TF0761:TF0762:conserved hypothetical protein:chromate transport protein:->->:809141..809263 123 tattatctataatccgtttatttgttggattttgaggcacaaaggtagataattttcccggatatcgttttttttatgtgtaaaatttgcatgattataccttaattatatttactttcgcat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0761:TF0762|TF0761:TF0762:conserved hypothetical protein:chromate transport protein:->->:809141..809263 123 tattatctataatccgtttatttgttggattttgaggcacaaaggtagataattttcccggatatcgttttttttatgtgtaaaatttgcatgattataccttaattatatttactttcgcat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tattatctataatccgtttatttgttggattttgaggcacaaaggtagataattttcccggatatcgttttttttatgtgtaaaatttgcatgattataccttaattatatttactttcgcat Predict ORF larger than 30AA ================================================ tattatctataatccgtttatttgttggattttgaggcacaaaggtagataattttcccggatatcgttttttttatgtgtaaaatttgcatgattataccttaattatatttactttcgcat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tattatctataatccgtttatttgttggattttgaggcacaaaggtagataattttcccggatatcgttttttttatgtgtaaaatttgcatgattataccttaattatatttactttcgcat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0761:TF0762|TF0761:TF0762:conserved hypothetical protein:chromate transport protein:->->:809141..809263 123 tattatctataatccgtttatttgttggattttgaggcacaaaggtagataattttcccggatatcgttttttttatgtgtaaaatttgcatgattataccttaattatatttactttcgcat Intra-Species Hit: Count: 1 Min: 1 Max: 123 Len: 123 Subject: tfor_TF0761_TF0762|conserved hypothetical protein:chromate transport protein|POSITIVE:POSITIVE|[809141,809263]|123 HSP 1 e-value: 1.0E-49 bit: 196.0 Len: 123 Query Start:1 Query End:123 Subject Strand: POSITIVE Subject Start: 1 Subject End: 123 tattatctataatccgtttatttgttggattttgaggcacaaaggtagataattttcccggatatcgnnnnnnnnatgtgtaaaatttgcatgattataccttaattatatttactttcgcat tattatctataatccgtttatttgttggattttgaggcacaaaggtagataattttcccggatatcgttttttttatgtgtaaaatttgcatgattataccttaattatatttactttcgcat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUUAUCUAUAAUCCGUUUAUUUGUUGGAUUUUGAGGCACAAAGGUAGAUAAUUUUCCCGGAUAUCGUUUUUUUUAUGUGUAAAAUUUGCAUGAUUAUACCUUAAUUAUAUUUACUUUCGCAU .....((...((((((.........))))))..)).((...((((((((((.......((.....))......(((((((.......)))))))..............)))))))))).)).. (-16.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUGCGAAAGUAAAUAUAAUUAAGGUAUAAUCAUGCAAAUUUUACACAUAAAAAAAACGAUAUCCGGGAAAAUUAUCUACCUUUGUGCCUCAAAAUCCAACAAAUAAACGGAUUAUAGAUAAUA .(((....)))..........((((((((.........(((((....))))).....((((...........)))).....))))))))...(((((...........))))).......... (-14.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 307 49 5 123 82 tfor:TF0801|5end_transport_permease_858586..858816_POSITIVE 307 60 13 119 63 tfor:TF0378|5end_aspartyl-tRNA_synthetase_397641..397871_POSITIVE 300 70 26 62 21 tfor:TF1339|5end_aspartate_1-decarboxylase_1420908..1421138_POSITIVE 298 62 23 210 174 tfor:TF1232|5end_hypothetical_protein_1317331..1317561_POSITIVE 285 51 12 60 11 tfor:TF1143|5end_ABC_transporter,_ATP-binding/permease_component_1221704..1221934_POSITIVE 283 67 24 112 74 tfor:TF0171|5end_hypothetical_protein_179946..180176_POSITIVE 272 80 41 132 93 tfor:TF0827|5end_conserved_hypothetical_protein_886240..886470_POSITIVE 269 60 14 224 183 tfor:TF2744|5end_methionyl-tRNA_formyltransferase_2926297..2926527_POSITIVE 265 78 33 152 96 tfor:TF2082|5end_DNA_topoisomerase_IV,_subunit_B_2248965..2249195_POSITIVE 260 75 31 149 107 tfor:TF2903|5end_translation_initiation_factor_SUI1_3098574..3098804_POSITIVE 260 67 22 146 100 tfor:TF1423|5end_cell_division_protein_(ftsW)_1505942..1506172_POSITIVE 260 48 6 132 87 tfor:TF0466|5end_amidophosphoribosyltransferase_483994..484224_POSITIVE 258 59 11 197 147 tfor:TF2567|5end_30S_ribosomal_protein_S14_2740867..2741097_POSITIVE 257 67 23 74 27 tfor:TF2341|5end_hypothetical_protein_2505735..2505965_POSITIVE 253 79 31 163 115 tfor:TF1717|5end_DNA_mismatch_repair_protein_1837805..1838035_POSITIVE 251 79 36 148 107 tfor:TF1189|5end_outer_membrane_protein,_TonB_dependent_receptor_1270586..1270816_POSITIVE 250 103 61 99 56 tfor:TF2565|5end_50S_ribosomal_protein_L24_2739986..2740216_POSITIVE 249 49 15 41 11 tfor:TF3021|5end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_3245437..3245667_POSITIVE 249 47 6 105 54 tfor:TF0676|5end_methyltransferase_719027..719257_POSITIVE 248 64 22 153 107 tfor:TF1796|5end_ABC_transporter,_ATP-binding_protein_1936492..1936722_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 286 80 36 49 2 tfor:TF0602|3end_aminotripeptidase;_peptidase_T_633472..633622_POSITIVE 272 80 41 102 63 tfor:TF0826|3end_primosomal_protein_N'_886290..886440_POSITIVE 265 78 33 138 82 tfor:TF2081|3end_phosphopantetheine_adenylyltransferase_2249031..2249181_POSITIVE 259 70 25 129 92 tfor:TF0874|3end_transposase_926652..926802_POSITIVE 251 79 36 116 75 tfor:TF1188|3end_conserved_hypothetical_protein_1270634..1270784_POSITIVE 250 103 61 79 36 tfor:TF2564|3end_50S_ribosomal_protein_L14_2740046..2740196_POSITIVE 248 64 22 57 11 tfor:TF1795|3end_conserved_hypothetical_protein_1936476..1936626_POSITIVE 244 67 34 42 4 tfor:TF2339|3end_hypothetical_protein_2505783..2505933_POSITIVE 243 81 48 145 112 tfor:TF2951|3end_O-succinylbenzoic_acid--CoA_ligase_3159622..3159772_POSITIVE 240 94 53 122 94 tfor:TF2483|3end_hypothetical_protein_2660022..2660172_POSITIVE 239 68 23 71 31 tfor:TF2111|3end_RNA_polymerase_sigma-54_factor_2282115..2282265_POSITIVE 238 56 15 89 44 tfor:TF0590|3end_hypothetical_protein_625429..625579_POSITIVE 236 60 13 131 83 tfor:TF0773|3end_outer_membrane_efflux_protein_822686..822836_POSITIVE 234 49 14 127 83 tfor:TF2112|3end_glycine_cleavage_H_protein_2282573..2282723_POSITIVE 232 69 21 84 35 tfor:TF2871|3end_hypothetical_protein_3062714..3062864_POSITIVE 232 89 47 69 26 tfor:TF2398|3end_magnesium_chelatase,_D/I_family_2571206..2571356_POSITIVE 232 98 53 61 12 tfor:TF0802|3end_glucose-1-phosphate_cytidylyltransferase_861082..861232_POSITIVE 231 63 22 106 61 tfor:TF2898|3end_conserved_hypothetical_protein_3087593..3087743_POSITIVE 228 70 23 124 58 tfor:TF1711|3end_beta-D-glucoside_glucohydrolase,_periplasmic_1832472..1832622_POSITIVE 226 78 32 70 15 tfor:TF1734|3end_conserved_hypothetical_protein_1860118..1860268_POSITIVE