Origin IGS: tggtataacgtttatttacgaccgtaaaagtacgaaaggatttgggaaaaacaaaacgagtctttacttttgctttccgtaacacgaagatatcaataagataatgtatataaaaacaagatttttctctttacatgcgctcttcgcagtcatgacacacaggcaaccgtctgcccaataacgatacgag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctcgtatcgttattgggcagacggttgcctgtgtgtcatgactgcgaagagcgcatgtaaagagaaaaatcttgtttttatatacattatcttattgatatcttcgtgttacggaaagcaaaagtaaagactcgttttgtttttcccaaatcctttcgtacttttacggtcgtaaataaacgttatacca Mask Tandem Repeat Region ================================================ tggtataacgtttatttacgaccgtaaaagtacgaaaggatttgggaaaaacaaaacgagtctttacttttgctttccgtaacacgaagatatcaataagataatgtatataaaaacaagatttttctctttacatgcgctcttcgcagtcatgacacacaggcaaccgtctgcccaataacgatacgag Find is-nt database================================================ Query_seq: TF0751:TF0752|TF0751:TF0752:dipeptidyl peptidase IV:nucleotide-binding protein; MAF-like protein:->->:800891..801080 190 tggtataacgtttatttacgaccgtaaaagtacgaaaggatttgggaaaaacaaaacgagtctttacttttgctttccgtaacacgaagatatcaataagataatgtatataaaaacaagatttttctctttacatgcgctcttcgcagtcatgacacacaggcaaccgtctgcccaataacgatacgag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0751:TF0752|TF0751:TF0752:dipeptidyl peptidase IV:nucleotide-binding protein; MAF-like protein:->->:800891..801080 190 tggtataacgtttatttacgaccgtaaaagtacgaaaggatttgggaaaaacaaaacgagtctttacttttgctttccgtaacacgaagatatcaataagataatgtatataaaaacaagatttttctctttacatgcgctcttcgcagtcatgacacacaggcaaccgtctgcccaataacgatacgag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0751:TF0752|TF0751:TF0752:dipeptidyl peptidase IV:nucleotide-binding protein; MAF-like protein:->->:800891..801080 190 tggtataacgtttatttacgaccgtaaaagtacgaaaggatttgggaaaaacaaaacgagtctttacttttgctttccgtaacacgaagatatcaataagataatgtatataaaaacaagatttttctctttacatgcgctcttcgcagtcatgacacacaggcaaccgtctgcccaataacgatacgag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tggtataacgtttatttacgaccgtaaaagtacgaaaggatttgggaaaaacaaaacgagtctttacttttgctttccgtaacacgaagatatcaataagataatgtatataaaaacaagatttttctctttacatgcgctcttcgcagtcatgacacacaggcaaccgtctgcccaataacgatacgag Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: - Start: 67 End: 162 .................................................................. K Q K G Y C S S I L L I I Y I F V L N K E K C A S K A T M V C M ............................ Protein_Len: 36 Strand: - Start: 2 End: 109 . T Y R K N V V T F T R F S K P F V F R T K V K A K R L V F I D I L Y H M ................................................................................. tggtataacgtttatttacgaccgtaaaagtacgaaaggatttgggaaaaacaaaacgagtctttacttttgctttccgtaacacgaagatatcaataagataatgtatataaaaacaagatttttctctttacatgcgctcttcgcagtcatgacacacaggcaaccgtctgcccaataacgatacgag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tggtataacgtttatttacgaccgtaaaagtacgaaaggatttgggaaaaacaaaacgagtctttacttttgctttccgtaacacgaagatatcaataagataatgtatataaaaacaagatttttctctttacatgcgctcttcgcagtcatgacacacaggcaaccgtctgcccaataacgatacgag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0751:TF0752|TF0751:TF0752:dipeptidyl peptidase IV:nucleotide-binding protein; MAF-like protein:->->:800891..801080 190 tggtataacgtttatttacgaccgtaaaagtacgaaaggatttgggaaaaacaaaacgagtctttacttttgctttccgtaacacgaagatatcaataagataatgtatataaaaacaagatttttctctttacatgcgctcttcgcagtcatgacacacaggcaaccgtctgcccaataacgatacgag Intra-Species Hit: Count: 1 Min: 1 Max: 190 Len: 190 Subject: tfor_TF0751_TF0752|dipeptidyl peptidase IV:nucleotide-binding protein; MAF-like protein|POSITIVE:POSITIVE|[800891,801080]|190 HSP 1 e-value: 1.0E-104 bit: 377.0 Len: 190 Query Start:1 Query End:190 Subject Strand: POSITIVE Subject Start: 1 Subject End: 190 tggtataacgtttatttacgaccgtaaaagtacgaaaggatttgggaaaaacaaaacgagtctttacttttgctttccgtaacacgaagatatcaataagataatgtatataaaaacaagatttttctctttacatgcgctcttcgcagtcatgacacacaggcaaccgtctgcccaataacgatacgag tggtataacgtttatttacgaccgtaaaagtacgaaaggatttgggaaaaacaaaacgagtctttacttttgctttccgtaacacgaagatatcaataagataatgtatataaaaacaagatttttctctttacatgcgctcttcgcagtcatgacacacaggcaaccgtctgcccaataacgatacgag Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGGUAUAACGUUUAUUUACGACCGUAAAAGUACGAAAGGAUUUGGGAAAAACAAAACGAGUCUUUACUUUUGCUUUCCGUAACACGAAGAUAUCAAUAAGAUAAUGUAUAUAAAAACAAGAUUUUUCUCUUUACAUGCGCUCUUCGCAGUCAUGACACACAGGCAACCGUCUGCCCAAUAACGAUACGAG ..((((..((((.......(((.(((((((((....((((((((............)))))))))))))))))...........((((((((((.....))))..((((.((((....(((....))).)))).))))..))))))..)))........(((((....)))))......))))))))... (-35.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUCGUAUCGUUAUUGGGCAGACGGUUGCCUGUGUGUCAUGACUGCGAAGAGCGCAUGUAAAGAGAAAAAUCUUGUUUUUAUAUACAUUAUCUUAUUGAUAUCUUCGUGUUACGGAAAGCAAAAGUAAAGACUCGUUUUGUUUUUCCCAAAUCCUUUCGUACUUUUACGGUCGUAAAUAAACGUUAUACCA ...((((((((....((((.((((....)))).))))..(((((((((((..(.(((((((((.((.....)).))))))))).)..((((.....))))))))))......((((((((((((........).))))))))))).....................))))).......))))..)))).. (-39.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 389 174 134 142 103 tfor:TF2205|5end_conserved_hypothetical_protein;_possible_transcriptional_regulator_2379055..2379285_POSITIVE 365 175 133 58 21 tfor:TF2801|5end_outer_membrane_protein,_TonB_dependent_receptor_2986285..2986515_POSITIVE 359 176 133 169 112 tfor:TF0013|5end_conserved_hypothetical_protein;_possible_TPR-repeat-containing_protein_6414..6644_POSITIVE 344 174 134 58 16 tfor:TF1170|5end_conserved_hypothetical_protein_1251862..1252092_POSITIVE 344 174 134 46 4 tfor:TF1171|5end_ABC_transporter,_ATP-binding_protein_1251850..1252080_POSITIVE 333 176 135 49 5 tfor:TF2429|5end_ABC_transporter,_ATP-binding/permease_component,_bacteriocin/lantibiotic_exporter_2610453..2610683_POSITIVE 333 170 127 192 155 tfor:TF0949|5end_conserved_hypothetical_protein_993863..994093_POSITIVE 330 179 134 72 8 tfor:TF1297|5end_hypothetical_protein_1379024..1379254_POSITIVE 330 184 145 33 1 tfor:TF0799|5end_conserved_hypothetical_protein_855191..855421_POSITIVE 329 190 142 61 10 tfor:TF2014|5end_dipeptidyl_aminopeptidase_IV_2169398..2169628_POSITIVE 327 177 134 206 166 tfor:TF3022|5end_chorismate_synthase_3246136..3246366_POSITIVE 326 176 137 78 44 tfor:TF1885|5end_conserved_hypothetical_protein_2037307..2037537_POSITIVE 322 173 135 59 5 tfor:TF0411|5end_methyltransferase_428917..429147_POSITIVE 320 180 139 66 25 tfor:TF0831|5end_conserved_hypothetical_protein_888183..888413_POSITIVE 318 176 136 43 1 tfor:TF2264|5end_transfer_region-related_protein,_TraO_2432890..2433120_POSITIVE 313 177 134 194 154 tfor:TF0147|5end_patatin-like_phospholipase_156798..157028_POSITIVE 312 175 138 71 31 tfor:TF2833|5end_lipid_A_disaccharide_synthase_3023973..3024203_POSITIVE 312 178 137 224 176 tfor:TF2775|5end_conserved_hypothetical_protein;_possible_mucoidy_inhibitor-related_protein_2959668..2959898_POSITIVE 310 176 132 145 102 tfor:TF2375|5end_conserved_hypothetical_protein_2540953..2541183_POSITIVE 309 187 141 220 171 tfor:TF2484|5end_hypothetical_protein_2660005..2660235_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 389 174 134 129 90 tfor:TF2204|3end_conserved_hypothetical_protein_2379122..2379272_POSITIVE 365 175 133 39 2 tfor:TF2800|3end_anti-sigma_factor_2986346..2986496_POSITIVE 320 180 139 53 12 tfor:TF0829|3end_conserved_hypothetical_protein_888250..888400_POSITIVE 316 176 138 41 1 tfor:TF2428|3end_conserved_hypothetical_protein_2610525..2610675_POSITIVE 298 174 135 106 59 tfor:TF3039|3end_ABC_transporter,_ATP-binding_protein_3267697..3267847_POSITIVE 296 185 142 62 14 tfor:TF2757|3end_hypothetical_protein_2942335..2942485_POSITIVE 295 186 141 65 1 tfor:TF2270|3end_conserved_hypothetical_protein_2435761..2435911_POSITIVE 294 178 134 64 6 tfor:TF2384|3end_thiol:disulfide_interchange_protein_2550756..2550906_POSITIVE 294 175 136 66 23 tfor:TF2344|3end_ATP/GTP_binding_protein_2508434..2508584_POSITIVE 294 187 141 75 13 tfor:TF1706|3end_conserved_hypothetical_protein_1828506..1828656_POSITIVE 290 176 139 37 1 tfor:TF2263|3end_transfer_region-related_protein,_TraN_2432964..2433114_POSITIVE 285 189 141 70 23 tfor:TF2978|3end_conserved_hypothetical_protein_3190862..3191012_POSITIVE 284 174 136 59 19 tfor:TF2883|3end_peptidyl-prolyl_cis-trans_isomerase_3075129..3075279_POSITIVE 284 94 57 50 3 tfor:TF1012|3end_lysyl-tRNA_synthetase_(lysine--tRNA_ligase)_1070056..1070206_POSITIVE 282 164 133 59 21 tfor:TF0037|3end_sialic_acid-specific_9-O-acetylesterase_39991..40141_POSITIVE 278 176 135 109 70 tfor:TF1914|3end_conserved_hypothetical_protein_2067656..2067806_POSITIVE 277 170 128 57 11 tfor:TF1064|3end_ampG_permease_1135382..1135532_POSITIVE 276 178 134 129 90 tfor:TF2372|3end_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosami_ne_acyltransferase_2538417..2538567_POSITIVE 276 173 133 63 16 tfor:TF0746|3end_possible_DNA_polymerase_III_792910..793060_POSITIVE 275 174 136 65 31 tfor:TF2132|3end_TIM-barrel_protein,_NifR3_family_2308769..2308919_POSITIVE