Origin IGS: tgatggcaaattacgcgatacgcggttcggcgtgtactacgataacaataaaccattaacaattattacgtcaggcttacagccctgtgaggggggagtgattttttttcttccaacgcttcgcattgggctgaatgaacacaccctttcaaggcttagaagagttagtcagcccttagcgttcagccaacaaatcaacaactaaacaaatcaacaactaaaccaatcaacaactaaacgaacgaggggcttacaagggctgaaagtccgatttatttcacattcccgaagcagagaaccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggttctctgcttcgggaatgtgaaataaatcggactttcagcccttgtaagcccctcgttcgtttagttgttgattggtttagttgttgatttgtttagttgttgatttgttggctgaacgctaagggctgactaactcttctaagccttgaaagggtgtgttcattcagcccaatgcgaagcgttggaagaaaaaaaatcactcccccctcacagggctgtaagcctgacgtaataattgttaatggtttattgttatcgtagtacacgccgaaccgcgtatcgcgtaatttgccatca Mask Tandem Repeat Region ================================================ tgatggcaaattacgcgatacgcggttcggcgtgtactacgataacaataaaccattaacaattattacgtcaggcttacagccctgtgaggggggagtgattttttttcttccaacgcttcgcattgggctgaatgaacacaccctttcaaggcttagaagagttagtcagcccttagcgttcagccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngaacgaggggcttacaagggctgaaagtccgatttatttcacattcccgaagcagagaaccta Find is-nt database================================================ Query_seq: TF0724:TF0725|TF0724:TF0725:possible transcriptional regulator:O-acetylhomoserine (thiol)-lyase:->->:776394..776695 302 tgatggcaaattacgcgatacgcggttcggcgtgtactacgataacaataaaccattaacaattattacgtcaggcttacagccctgtgaggggggagtgattttttttcttccaacgcttcgcattgggctgaatgaacacaccctttcaaggcttagaagagttagtcagcccttagcgttcagccaacaaatcaacaactaaacaaatcaacaactaaaccaatcaacaactaaacgaacgaggggcttacaagggctgaaagtccgatttatttcacattcccgaagcagagaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0724:TF0725|TF0724:TF0725:possible transcriptional regulator:O-acetylhomoserine (thiol)-lyase:->->:776394..776695 302 tgatggcaaattacgcgatacgcggttcggcgtgtactacgataacaataaaccattaacaattattacgtcaggcttacagccctgtgaggggggagtgattttttttcttccaacgcttcgcattgggctgaatgaacacaccctttcaaggcttagaagagttagtcagcccttagcgttcagccaacaaatcaacaactaaacaaatcaacaactaaaccaatcaacaactaaacgaacgaggggcttacaagggctgaaagtccgatttatttcacattcccgaagcagagaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0724:TF0725|TF0724:TF0725:possible transcriptional regulator:O-acetylhomoserine (thiol)-lyase:->->:776394..776695 302 tgatggcaaattacgcgatacgcggttcggcgtgtactacgataacaataaaccattaacaattattacgtcaggcttacagccctgtgaggggggagtgattttttttcttccaacgcttcgcattgggctgaatgaacacaccctttcaaggcttagaagagttagtcagcccttagcgttcagccaacaaatcaacaactaaacaaatcaacaactaaaccaatcaacaactaaacgaacgaggggcttacaagggctgaaagtccgatttatttcacattcccgaagcagagaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgatggcaaattacgcgatacgcggttcggcgtgtactacgataacaataaaccattaacaattattacgtcaggcttacagccctgtgaggggggagtgattttttttcttccaacgcttcgcattgggctgaatgaacacaccctttcaaggcttagaagagttagtcagcccttagcgttcagccaacaaatcaacaactaaacaaatcaacaactaaaccaatcaacaactaaacgaacgaggggcttacaagggctgaaagtccgatttatttcacattcccgaagcagagaaccta Predict ORF larger than 30AA ================================================ Protein_Len: 57 Strand: + Start: 32 End: 202 ............................... M Y Y D N N K P L T I I T S G L Q P C E G G V I F F L P T L R I G L N E H T L S R L R R V S Q P L A F S Q Q I N N .................................................................................................... Protein_Len: 35 Strand: - Start: 40 End: 144 ....................................... S L L L G N V I I V D P K C G Q S P P T I K K R G V S R M P S F S C M .............................................................................................................................................................. tgatggcaaattacgcgatacgcggttcggcgtgtactacgataacaataaaccattaacaattattacgtcaggcttacagccctgtgaggggggagtgattttttttcttccaacgcttcgcattgggctgaatgaacacaccctttcaaggcttagaagagttagtcagcccttagcgttcagccaacaaatcaacaactaaacaaatcaacaactaaaccaatcaacaactaaacgaacgaggggcttacaagggctgaaagtccgatttatttcacattcccgaagcagagaaccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgatggcaaattacgcgatacgcggttcggcgtgtactacgataacaataaaccattaacaattattacgtcaggcttacagccctgtgaggggggagtgattttttttcttccaacgcttcgcattgggctgaatgaacacaccctttcaaggcttagaagagttagtcagcccttagcgttcagccaacaaatcaacaactaaacaaatcaacaactaaaccaatcaacaactaaacgaacgaggggcttacaagggctgaaagtccgatttatttcacattcccgaagcagagaaccta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0724:TF0725|TF0724:TF0725:possible transcriptional regulator:O-acetylhomoserine (thiol)-lyase:->->:776394..776695 302 tgatggcaaattacgcgatacgcggttcggcgtgtactacgataacaataaaccattaacaattattacgtcaggcttacagccctgtgaggggggagtgattttttttcttccaacgcttcgcattgggctgaatgaacacaccctttcaaggcttagaagagttagtcagcccttagcgttcagccaacaaatcaacaactaaacaaatcaacaactaaaccaatcaacaactaaacgaacgaggggcttacaagggctgaaagtccgatttatttcacattcccgaagcagagaaccta Intra-Species Hit: Count: 1 Min: 1 Max: 302 Len: 302 Subject: tfor_TF0724_TF0725|possible transcriptional regulator:O-acetylhomoserine (thiol)-lyase|POSITIVE:POSITIVE|[776394,776695]|302 HSP 1 e-value: 2.0E-87 bit: 323.0 Len: 187 Query Start:1 Query End:187 Subject Strand: POSITIVE Subject Start: 1 Subject End: 187 tgatggcaaattacgcgatacgcggttcggcgtgtactacgataacaataaaccattaacaattattacgtcaggcttacagccctgtgaggggggagtgannnnnnnncttccaacgcttcgcattgggctgaatgaacacaccctttcaaggcttagaagagttagtcagcccttagcgttcagc................................................................................................................... tgatggcaaattacgcgatacgcggttcggcgtgtactacgataacaataaaccattaacaattattacgtcaggcttacagccctgtgaggggggagtgattttttttcttccaacgcttcgcattgggctgaatgaacacaccctttcaaggcttagaagagttagtcagcccttagcgttcagc................................................................................................................... HSP 2 e-value: 2.0E-25 bit: 117.0 Len: 59 Query Start:244 Query End:302 Subject Strand: POSITIVE Subject Start: 244 Subject End: 302 ...................................................................................................................................................................................................................................................gaggggcttacaagggctgaaagtccgatttatttcacattcccgaagcagagaaccta ...................................................................................................................................................................................................................................................gaggggcttacaagggctgaaagtccgatttatttcacattcccgaagcagagaaccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUGGCAAAUUACGCGAUACGCGGUUCGGCGUGUACUACGAUAACAAUAAACCAUUAACAAUUAUUACGUCAGGCUUACAGCCCUGUGAGGGGGGAGUGAUUUUUUUUCUUCCAACGCUUCGCAUUGGGCUGAAUGAACACACCCUUUCAAGGCUUAGAAGAGUUAGUCAGCCCUUAGCGUUCAGCCAACAAAUCAACAACUAAACAAAUCAACAACUAAACCAAUCAACAACUAAACGAACGAGGGGCUUACAAGGGCUGAAAGUCCGAUUUAUUUCACAUUCCCGAAGCAGAGAACCUA .....((......((((...))))..((((.((((...........(((((...........)))))..(((.(((((.(((((((.((((((((..(((.(((...(((.(((((.((...)).)))))..))).)))))).)))))))).(((((....))))).((.((((((...(((((.......................................................))))).)))))).)).))))))).))))).))).......))))..)))).)).......... (-71.36) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGUUCUCUGCUUCGGGAAUGUGAAAUAAAUCGGACUUUCAGCCCUUGUAAGCCCCUCGUUCGUUUAGUUGUUGAUUGGUUUAGUUGUUGAUUUGUUUAGUUGUUGAUUUGUUGGCUGAACGCUAAGGGCUGACUAACUCUUCUAAGCCUUGAAAGGGUGUGUUCAUUCAGCCCAAUGCGAAGCGUUGGAAGAAAAAAAAUCACUCCCCCCUCACAGGGCUGUAAGCCUGACGUAAUAAUUGUUAAUGGUUUAUUGUUAUCGUAGUACACGCCGAACCGCGUAUCGCGUAAUUUGCCAUCA (((((((((((....(((...((((..............((((((((((((((((..(((..((......)).)))..))))))...........(((((((((.........)))))))))..)))))))))).....((((((((((.(((...((((.((......))))))....))).)).)))))))).......))))...)))....))))).....))))))...............(((((.....((((.((..((((.((......)).))))..))))))..))))).. (-79.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 691 150 101 184 136 tfor:TF2824|5end_nucleoside-transporting_protein_nupG_3014701..3014931_POSITIVE 632 156 111 78 33 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 616 157 111 200 153 tfor:TF0828|5end_hypothetical_protein_887090..887320_POSITIVE 598 157 111 194 147 tfor:TF1186|5end_hypothetical_protein_1269568..1269798_POSITIVE 598 157 111 190 143 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 594 156 111 216 170 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 589 156 111 116 70 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 589 156 111 78 32 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 588 156 111 219 169 tfor:TF2478|5end_hypothetical_protein_2655375..2655605_POSITIVE 581 157 111 124 67 tfor:TF0538|5end_hypothetical_protein_566891..567121_POSITIVE 581 157 111 117 60 tfor:TF0539|5end_hypothetical_protein_566884..567114_POSITIVE 554 136 93 231 190 tfor:TF0823|5end_conserved_hypothetical_protein_880660..880890_POSITIVE 548 136 94 230 187 tfor:TF1187|5end_hypothetical_protein_1269625..1269855_POSITIVE 547 120 71 65 16 tfor:TF1716|5end_hypothetical_protein_1837442..1837672_POSITIVE 544 138 91 203 160 tfor:TF2036|5end_possible_pyruvate_formate-lyase_activating_enzyme_2196302..2196532_POSITIVE 525 99 52 230 175 tfor:TF2816|5end_hypothetical_protein_3006925..3007155_POSITIVE 524 156 111 116 74 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 524 156 111 64 22 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 502 157 117 177 137 tfor:TF2517|5end_hypothetical_protein_2695089..2695319_POSITIVE 497 157 117 124 79 tfor:TF0534|5end_hypothetical_protein_559509..559739_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 632 156 111 61 16 tfor:TF0820|3end_hypothetical_protein_879942..880092_POSITIVE 600 156 111 82 27 tfor:TF2840|3end_hypothetical_protein_3031289..3031439_POSITIVE 598 157 111 109 62 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 594 156 111 70 24 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 538 138 94 128 83 tfor:TF2547|3end_hypothetical_protein_2730947..2731097_POSITIVE 510 46 1 52 6 tfor:TF3106|3end_hypothetical_protein_3338608..3338758_POSITIVE 497 157 117 54 9 tfor:TF0533|3end_hypothetical_protein_559519..559669_POSITIVE 493 89 43 99 50 tfor:TF2816|3end_hypothetical_protein_3007184..3007334_POSITIVE 478 97 59 148 110 tfor:TF0678|3end_hypothetical_protein_721335..721485_POSITIVE 416 187 142 85 38 tfor:TF0685|3end_hypothetical_protein_731132..731282_POSITIVE 410 209 161 69 22 tfor:TF1816|3end_hypothetical_protein_1957974..1958124_POSITIVE 410 209 161 86 39 tfor:TF1817|3end_hypothetical_protein_1957991..1958141_POSITIVE 389 130 81 91 33 tfor:TF2556|3end_conserved_hypothetical_protein_2737095..2737245_POSITIVE 381 95 52 66 19 tfor:TF0340|3end_hypothetical_protein_364257..364407_POSITIVE 374 33 7 148 122 tfor:TF0822|3end_hypothetical_protein_880764..880914_POSITIVE 361 130 81 121 77 tfor:TF1590|3end_hypothetical_protein_1706043..1706193_POSITIVE 361 120 71 112 51 tfor:TF1575|3end_DNA-binding_response_regulator_1685895..1686045_POSITIVE 360 230 183 60 13 tfor:TF2839|3end_ATP-dependent_exodeoxyribonuclease_3031035..3031185_POSITIVE 355 33 2 151 123 tfor:TF2710|3end_ABC_transporter,_ATP-binding_protein/permease_component_2884615..2884765_POSITIVE 349 208 164 101 58 tfor:TF0448|3end_hypothetical_protein_467911..468061_POSITIVE