Origin IGS: taacaacacgcaatctgttattcttttttaacaacaaaaagtctctttttttcttgttcttcttaaaaatacatcttatattgcccaccgaaattgaggattattcgtaattgtaaca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgttacaattacgaataatcctcaatttcggtgggcaatataagatgtatttttaagaagaacaagaaaaaaagagactttttgttgttaaaaaagaataacagattgcgtgttgtta Mask Tandem Repeat Region ================================================ taacaacacgcaatctgttattcttttttaacaacaaaaagtctctttttttcttgttcttcttaaaaatacatcttatattgcccaccgaaattgaggattattcgtaattgtaaca Find is-nt database================================================ Query_seq: TF0723:TF0724|TF0723:TF0724:possible aspartate aminotransferase:possible transcriptional regulator:->->:775685..775802 118 taacaacacgcaatctgttattcttttttaacaacaaaaagtctctttttttcttgttcttcttaaaaatacatcttatattgcccaccgaaattgaggattattcgtaattgtaaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0723:TF0724|TF0723:TF0724:possible aspartate aminotransferase:possible transcriptional regulator:->->:775685..775802 118 taacaacacgcaatctgttattcttttttaacaacaaaaagtctctttttttcttgttcttcttaaaaatacatcttatattgcccaccgaaattgaggattattcgtaattgtaaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0723:TF0724|TF0723:TF0724:possible aspartate aminotransferase:possible transcriptional regulator:->->:775685..775802 118 taacaacacgcaatctgttattcttttttaacaacaaaaagtctctttttttcttgttcttcttaaaaatacatcttatattgcccaccgaaattgaggattattcgtaattgtaaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacaacacgcaatctgttattcttttttaacaacaaaaagtctctttttttcttgttcttcttaaaaatacatcttatattgcccaccgaaattgaggattattcgtaattgtaaca Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 15 End: 107 .............. M L F F F N N K K S L F F L F F L K I H L I L P T E I E D Y S ........... Protein_Len: 31 Strand: - Start: 2 End: 94 . V V R L R N N K K L L L F D R K K K N K K F I C R I N G V S M ........................ taacaacacgcaatctgttattcttttttaacaacaaaaagtctctttttttcttgttcttcttaaaaatacatcttatattgcccaccgaaattgaggattattcgtaattgtaaca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacaacacgcaatctgttattcttttttaacaacaaaaagtctctttttttcttgttcttcttaaaaatacatcttatattgcccaccgaaattgaggattattcgtaattgtaaca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0723:TF0724|TF0723:TF0724:possible aspartate aminotransferase:possible transcriptional regulator:->->:775685..775802 118 taacaacacgcaatctgttattcttttttaacaacaaaaagtctctttttttcttgttcttcttaaaaatacatcttatattgcccaccgaaattgaggattattcgtaattgtaaca Intra-Species Hit: Count: 1 Min: 1 Max: 118 Len: 118 Subject: tfor_TF0723_TF0724|possible aspartate aminotransferase:possible transcriptional regulator|POSITIVE:POSITIVE|[775685,775802]|118 HSP 1 e-value: 2.0E-48 bit: 192.0 Len: 118 Query Start:1 Query End:118 Subject Strand: POSITIVE Subject Start: 1 Subject End: 118 taacaacacgcaatctgttattcttttttaacaacaaaaagtctcnnnnnnncttgttcttcttaaaaatacatcttatattgcccaccgaaattgaggattattcgtaattgtaaca taacaacacgcaatctgttattcttttttaacaacaaaaagtctctttttttcttgttcttcttaaaaatacatcttatattgcccaccgaaattgaggattattcgtaattgtaaca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACAACACGCAAUCUGUUAUUCUUUUUUAACAACAAAAAGUCUCUUUUUUUCUUGUUCUUCUUAAAAAUACAUCUUAUAUUGCCCACCGAAAUUGAGGAUUAUUCGUAAUUGUAACA ..((((...(((((.((.......((((((((((.((((((.....)))))).))))......)))))).......)).)))))...((........))...........)))).... (-10.64) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUUACAAUUACGAAUAAUCCUCAAUUUCGGUGGGCAAUAUAAGAUGUAUUUUUAAGAAGAACAAGAAAAAAAGAGACUUUUUGUUGUUAAAAAAGAAUAACAGAUUGCGUGUUGUUA ...........((((...........))))...((((((((((..(((((((((...((.((((((((..........)))))))).))....)))))).)))..))..)))))))). (-17.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 298 75 40 38 4 tfor:TF0078|5end_aminopeptidase_C,_peptidase_C1-like_family_84529..84759_POSITIVE 256 108 67 52 7 tfor:TF2712|5end_glycosyltransferase_2885918..2886148_POSITIVE 256 101 72 223 193 tfor:TF1619|5end_riboflavin_synthase,_beta_subunit_1733948..1734178_POSITIVE 230 96 51 167 114 tfor:TF2684|5end_conserved_hypothetical_protein;_possible_CvpA_family_protein_2860692..2860922_POSITIVE 224 90 41 180 137 tfor:TF2285|5end_hypothetical_protein_2440783..2441013_POSITIVE 222 89 41 226 177 tfor:TF2889|5end_50S_ribosomal_protein_L27_3078333..3078563_POSITIVE 222 79 32 134 86 tfor:TF0405|5end_conserved_hypothetical_protein_423767..423997_POSITIVE 215 110 82 48 25 tfor:TF1375|5end_conserved_hypothetical_protein_1451183..1451413_POSITIVE 215 107 81 224 192 tfor:TF0222|5end_conserved_hypothetical_protein_249269..249499_POSITIVE 210 64 42 145 124 tfor:TF2683|5end_translation_initiation_factor_IF-2_2857722..2857952_POSITIVE 210 100 54 228 184 tfor:TF0081|5end_pantothenate_kinase_87464..87694_POSITIVE 207 60 14 114 72 tfor:TF2311|5end_conserved_hypothetical_protein_2471290..2471520_POSITIVE 206 110 83 214 183 tfor:TF1284|5end_glyceraldehyde_3-phosphate_dehydrogenase_1367939..1368169_POSITIVE 206 109 71 153 117 tfor:TF0156|5end_conserved_hypothetical_protein_164973..165203_POSITIVE 205 110 72 174 130 tfor:TF2774|5end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_2959133..2959363_POSITIVE 204 110 61 179 118 tfor:TF2760|5end_MarR__family_transcriptional_regulator_2945019..2945249_POSITIVE 204 90 44 156 114 tfor:TF2638|5end_cation_transport_protein_2807985..2808215_POSITIVE 203 90 45 221 185 tfor:TF2677|5end_aspartate_transaminase_2850809..2851039_POSITIVE 202 108 73 231 192 tfor:TF2951|5end_O-succinylbenzoic_acid--CoA_ligase_3158496..3158726_POSITIVE 198 100 51 107 53 tfor:TF3160|5end_conserved_hypothetical_protein_3395531..3395761_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 256 108 67 59 14 tfor:TF2711|3end_hemolysis_secretion_protein_2886005..2886155_POSITIVE 230 96 51 151 98 tfor:TF2683|3end_translation_initiation_factor_IF-2_2860756..2860906_POSITIVE 222 79 32 134 86 tfor:TF0404|3end_conserved_hypothetical_protein_423847..423997_POSITIVE 210 64 42 113 92 tfor:TF2682|3end_N_utilization_substance_protein_A_2857770..2857920_POSITIVE 210 100 54 45 1 tfor:TF0080|3end_phosphate_ABC_transporter,_periplasmic_phosphate-binding_protein_87361..87511_POSITIVE 208 97 54 86 48 tfor:TF2721|3end_conserved_hypothetical_protein_2896956..2897106_POSITIVE 204 90 44 46 4 tfor:TF2637|3end_conserved_hypothetical_protein_2807955..2808105_POSITIVE 198 100 51 107 53 tfor:TF3159|3end_conserved_hypothetical_protein;_possible_transcriptional_regulator_3395611..3395761_POSITIVE 196 43 9 72 36 tfor:TF2297|3end_hypothetical_protein_2449673..2449823_POSITIVE 196 108 65 148 99 tfor:TF0587|3end_outer_membrane_protein_619931..620081_POSITIVE 193 106 65 124 76 tfor:TF0196|3end_conserved_hypothetical_protein_221037..221187_POSITIVE 192 69 40 59 33 tfor:TF2886|3end_RNA_methylase_SpoU_family_3076270..3076420_POSITIVE 190 98 54 48 4 tfor:TF3101|3end_hypothetical_protein_3332659..3332809_POSITIVE 190 61 24 40 1 tfor:TF0678|3end_hypothetical_protein_721335..721485_POSITIVE 189 85 43 146 110 tfor:TF1441|3end_conserved_hypothetical_protein_1526276..1526426_POSITIVE 188 100 74 36 3 tfor:TF1708|3end_transcriptional_regulator_1829513..1829663_POSITIVE 187 97 52 94 50 tfor:TF0408|3end_possible_gliding_motility-related_lipoprotein_426324..426474_POSITIVE 185 108 88 58 41 tfor:TF2009|3end_octaprenyl-diphosphate_synthase_ispB_2163245..2163395_POSITIVE 185 59 35 63 42 tfor:TF1547|3end_conserved_hypothetical_protein_1652471..1652621_POSITIVE 182 110 81 63 30 tfor:TF0516|3end_conserved_hypothetical_protein;_possible_membrane_protein_540693..540843_POSITIVE