Origin IGS: taatcatttcatcagaattatacgaaagacgaagcgaggtcgctatgcgcgggtttctcacacgatgtctcgatcggacagtttggcctacgaatcctcgccctctgtttccgttaaaaaaactatctttgcagactttataacgtgatgatacatcacatcaagtatccggaacgagtcgggaagaaatgttttttatcacatgatcac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gtgatcatgtgataaaaaacatttcttcccgactcgttccggatacttgatgtgatgtatcatcacgttataaagtctgcaaagatagtttttttaacggaaacagagggcgaggattcgtaggccaaactgtccgatcgagacatcgtgtgagaaacccgcgcatagcgacctcgcttcgtctttcgtataattctgatgaaatgatta Mask Tandem Repeat Region ================================================ taatcatttcatcagaattatacgaaagacgaagcgaggtcgctatgcgcgggtttctcacacgatgtctcgatcggacagtttggcctacgaatcctcgccctctgtttccgttaaaaaaactatctttgcagactttataacgtgatgatacatcacatcaagtatccggaacgagtcgggaagaaatgttttttatcacatgatcac Find is-nt database================================================ Query_seq: TF0705:TF0707|TF0705:TF0707:hypothetical protein:conserved hypothetical protein:->->:750850..751059 210 taatcatttcatcagaattatacgaaagacgaagcgaggtcgctatgcgcgggtttctcacacgatgtctcgatcggacagtttggcctacgaatcctcgccctctgtttccgttaaaaaaactatctttgcagactttataacgtgatgatacatcacatcaagtatccggaacgagtcgggaagaaatgttttttatcacatgatcac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0705:TF0707|TF0705:TF0707:hypothetical protein:conserved hypothetical protein:->->:750850..751059 210 taatcatttcatcagaattatacgaaagacgaagcgaggtcgctatgcgcgggtttctcacacgatgtctcgatcggacagtttggcctacgaatcctcgccctctgtttccgttaaaaaaactatctttgcagactttataacgtgatgatacatcacatcaagtatccggaacgagtcgggaagaaatgttttttatcacatgatcac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0705:TF0707|TF0705:TF0707:hypothetical protein:conserved hypothetical protein:->->:750850..751059 210 taatcatttcatcagaattatacgaaagacgaagcgaggtcgctatgcgcgggtttctcacacgatgtctcgatcggacagtttggcctacgaatcctcgccctctgtttccgttaaaaaaactatctttgcagactttataacgtgatgatacatcacatcaagtatccggaacgagtcgggaagaaatgttttttatcacatgatcac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatcatttcatcagaattatacgaaagacgaagcgaggtcgctatgcgcgggtttctcacacgatgtctcgatcggacagtttggcctacgaatcctcgccctctgtttccgttaaaaaaactatctttgcagactttataacgtgatgatacatcacatcaagtatccggaacgagtcgggaagaaatgttttttatcacatgatcac Predict ORF larger than 30AA ================================================ Protein_Len: 46 Strand: + Start: 3 End: 140 .. M I S S E L Y E R R S E V A M R G F L T R C L D R T V W P T N P R P L F P L K K L S L Q T L ...................................................................... Protein_Len: 45 Strand: + Start: 11 End: 145 .......... M R I I R K T K R G R Y A R V S H T M S R S D S L A Y E S S P S V S V K K T I F A D F I T ................................................................. taatcatttcatcagaattatacgaaagacgaagcgaggtcgctatgcgcgggtttctcacacgatgtctcgatcggacagtttggcctacgaatcctcgccctctgtttccgttaaaaaaactatctttgcagactttataacgtgatgatacatcacatcaagtatccggaacgagtcgggaagaaatgttttttatcacatgatcac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 17 End: 46 ................ATAGCGACCTCGCTTCGTCTTTCGTATAAT.................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatcatttcatcagaattatacgaaagacgaagcgaggtcgctatgcgcgggtttctcacacgatgtctcgatcggacagtttggcctacgaatcctcgccctctgtttccgttaaaaaaactatctttgcagactttataacgtgatgatacatcacatcaagtatccggaacgagtcgggaagaaatgttttttatcacatgatcac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0705:TF0707|TF0705:TF0707:hypothetical protein:conserved hypothetical protein:->->:750850..751059 210 taatcatttcatcagaattatacgaaagacgaagcgaggtcgctatgcgcgggtttctcacacgatgtctcgatcggacagtttggcctacgaatcctcgccctctgtttccgttaaaaaaactatctttgcagactttataacgtgatgatacatcacatcaagtatccggaacgagtcgggaagaaatgttttttatcacatgatcac Intra-Species Hit: Count: 1 Min: 1 Max: 210 Len: 210 Subject: tfor_TF0705_TF0707|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[750850,751059]|210 HSP 1 e-value: 1.0E-103 bit: 375.0 Len: 210 Query Start:1 Query End:210 Subject Strand: POSITIVE Subject Start: 1 Subject End: 210 taatcatttcatcagaattatacgaaagacgaagcgaggtcgctatgcgcgggtttctcacacgatgtctcgatcggacagtttggcctacgaatcctcgccctctgtttccgttnnnnnnnctatctttgcagactttataacgtgatgatacatcacatcaagtatccggaacgagtcgggaagaaatgttttttatcacatgatcac taatcatttcatcagaattatacgaaagacgaagcgaggtcgctatgcgcgggtttctcacacgatgtctcgatcggacagtttggcctacgaatcctcgccctctgtttccgttaaaaaaactatctttgcagactttataacgtgatgatacatcacatcaagtatccggaacgagtcgggaagaaatgttttttatcacatgatcac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCAUUUCAUCAGAAUUAUACGAAAGACGAAGCGAGGUCGCUAUGCGCGGGUUUCUCACACGAUGUCUCGAUCGGACAGUUUGGCCUACGAAUCCUCGCCCUCUGUUUCCGUUAAAAAAACUAUCUUUGCAGACUUUAUAACGUGAUGAUACAUCACAUCAAGUAUCCGGAACGAGUCGGGAAGAAAUGUUUUUUAUCACAUGAUCAC ..(((((.....((((......(....).....((((((........((..((((.((....((((......))))...))...))))..))...))))))..)))).........(((((((..(((((.(.(((((......((((((...)))))).((........))...)))))).)))))...))))))).....)))))... (-45.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUGAUCAUGUGAUAAAAAACAUUUCUUCCCGACUCGUUCCGGAUACUUGAUGUGAUGUAUCAUCACGUUAUAAAGUCUGCAAAGAUAGUUUUUUUAACGGAAACAGAGGGCGAGGAUUCGUAGGCCAAACUGUCCGAUCGAGACAUCGUGUGAGAAACCCGCGCAUAGCGACCUCGCUUCGUCUUUCGUAUAAUUCUGAUGAAAUGAUUA .(((((((((((.........(((((..((((((((...((((((..((((((((((...))))))))))....((((((.((....(((((((....(....)))))))).....)).)))))).....))))))..))))...))).)..)))))..(((.....)))...)))).(((((..............))))).))))))) (-53.24) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 405 87 42 200 147 tfor:TF2520|5end_conserved_hypothetical_protein_2698917..2699147_POSITIVE 393 65 29 226 194 tfor:TF0064|5end_conserved_hypothetical_protein_68419..68649_POSITIVE 391 88 41 205 154 tfor:TF2572|5end_50S_ribosomal_protein_L30_2743081..2743311_POSITIVE 387 68 23 48 1 tfor:TF1846|5end_hypothetical_protein_1996726..1996956_POSITIVE 385 88 47 141 83 tfor:TF2674|5end_conserved_hypothetical_protein;_possible_phosphoesterase_2848176..2848406_POSITIVE 385 70 23 166 116 tfor:TF1804|5end_hypothetical_protein_1947101..1947331_POSITIVE 376 65 31 226 196 tfor:TF0066|5end_conserved_hypothetical_protein_70729..70959_POSITIVE 374 70 24 161 105 tfor:TF0206|5end_conserved_hypothetical_protein_232218..232448_POSITIVE 371 116 71 50 1 tfor:TF2228|5end_CTn_excision_protein_2395899..2396129_POSITIVE 368 108 63 73 29 tfor:TF2310|5end_dehydrogenase,_possible_3-oxoacyl-[acyl-carrier-protein]_reductase_2470481..2470711_POSITIVE 359 77 34 221 157 tfor:TF2704|5end_ribonuclease_HII_2876242..2876472_POSITIVE 359 60 13 144 93 tfor:TF1808|5end_DNA_processing_protein_subunit_A_1949356..1949586_POSITIVE 358 110 62 192 143 tfor:TF2782|5end_conserved_hypothetical_protein_2966455..2966685_POSITIVE 355 79 33 59 7 tfor:TF3072|5end_conserved_hypothetical_protein_3295302..3295532_POSITIVE 354 107 63 199 152 tfor:TF0765|5end_conserved_hypothetical_protein_811302..811532_POSITIVE 353 112 71 124 80 tfor:TF1317|5end_hypothetical_protein_1391378..1391608_POSITIVE 353 80 35 227 185 tfor:TF0696|5end_3-oxoacyl-(acyl-carrier-protein)_reductase_739936..740166_POSITIVE 352 65 23 47 10 tfor:TF2884|5end_conserved_hypothetical_protein_3075057..3075287_POSITIVE 350 88 43 67 21 tfor:TF2358|5end_possible_small-conductance_mechanosensitive_channel_2523829..2524059_POSITIVE 348 109 63 138 89 tfor:TF2047|5end_hypothetical_protein_2210057..2210287_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 385 70 23 105 55 tfor:TF1803|3end_conserved_hypothetical_protein_1947120..1947270_POSITIVE 383 77 34 141 76 tfor:TF2673|3end_conserved_hypothetical_protein_2848272..2848422_POSITIVE 371 68 24 148 96 tfor:TF0205|3end_exsB_protein_232289..232439_POSITIVE 359 60 13 133 82 tfor:TF1807|3end_thioesterase_family_protein_1949425..1949575_POSITIVE 355 79 33 59 7 tfor:TF3071|3end_integral_membrane_protein,_possible_MotA/TolQ/ExbB_proton_channel_family_3295382..3295532_POSITIVE 352 65 23 39 2 tfor:TF2883|3end_peptidyl-prolyl_cis-trans_isomerase_3075129..3075279_POSITIVE 352 85 41 136 83 tfor:TF0598|3end_hypothetical_protein_629500..629650_POSITIVE 350 88 43 58 12 tfor:TF2357|3end_collagenase_2523900..2524050_POSITIVE 347 89 47 63 17 tfor:TF0774|3end_membrane-fusion_protein;_36_kDa_antigen_823730..823880_POSITIVE 344 103 62 103 48 tfor:TF2392|3end_alpha-galactosidase_2565506..2565656_POSITIVE 340 80 34 84 38 tfor:TF2336|3end_conserved_hypothetical_protein_2498159..2498309_POSITIVE 337 114 71 147 103 tfor:TF2745|3end_hypothetical_protein_2927947..2928097_POSITIVE 337 88 41 67 22 tfor:TF0235|3end_hypothetical_protein_261849..261999_POSITIVE 336 79 31 64 5 tfor:TF1900|3end_conserved_hypothetical_protein_2056532..2056682_POSITIVE 336 113 71 67 21 tfor:TF0095|3end_methyltransferase_106845..106995_POSITIVE 334 64 28 39 6 tfor:TF0440|3end_Na+-transporting_NADH:ubiquinone_oxidoreductase,_subunit_2_464688..464838_POSITIVE 333 76 34 151 103 tfor:TF2046|3end_polyribonucleotide_nucleotidyltransferase_2210152..2210302_POSITIVE 332 136 91 102 58 tfor:TF1472|3end_acyl-ACP_thioesterase_1566337..1566487_POSITIVE 332 107 62 77 26 tfor:TF1066|3end_hypothetical_protein_1136858..1137008_POSITIVE 329 86 41 45 3 tfor:TF1737|3end_outer_membrane_protein,_TonB_dependent_receptor_1865306..1865456_POSITIVE