Origin IGS: aaaaattctcttccgttatttcctgctccataccagctacgacaaagactgacatgcaaccggtcatcatataaattcgtttcataaaatggggagtatttaatgagtaaataaaaatatcatccgccatacgaacta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagttcgtatggcggatgatatttttatttactcattaaatactccccattttatgaaacgaatttatatgatgaccggttgcatgtcagtctttgtcgtagctggtatggagcaggaaataacggaagagaattttt Mask Tandem Repeat Region ================================================ aaaaattctcttccgttatttcctgctccataccagctacgacaaagactgacatgcaaccggtcatcatataaattcgtttcataaaatggggagtatttaatgagtaaataaaaatatcatccgccatacgaacta Find is-nt database================================================ Query_seq: TF0688:TF0689|TF0688:TF0689:membrane-associated protein, possible sulfatase family:hypothetical protein:->->:734421..734558 138 aaaaattctcttccgttatttcctgctccataccagctacgacaaagactgacatgcaaccggtcatcatataaattcgtttcataaaatggggagtatttaatgagtaaataaaaatatcatccgccatacgaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0688:TF0689|TF0688:TF0689:membrane-associated protein, possible sulfatase family:hypothetical protein:->->:734421..734558 138 aaaaattctcttccgttatttcctgctccataccagctacgacaaagactgacatgcaaccggtcatcatataaattcgtttcataaaatggggagtatttaatgagtaaataaaaatatcatccgccatacgaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0688:TF0689|TF0688:TF0689:membrane-associated protein, possible sulfatase family:hypothetical protein:->->:734421..734558 138 aaaaattctcttccgttatttcctgctccataccagctacgacaaagactgacatgcaaccggtcatcatataaattcgtttcataaaatggggagtatttaatgagtaaataaaaatatcatccgccatacgaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaaattctcttccgttatttcctgctccataccagctacgacaaagactgacatgcaaccggtcatcatataaattcgtttcataaaatggggagtatttaatgagtaaataaaaatatcatccgccatacgaacta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: - Start: 3 End: 104 .. F E R G N N G A G Y W S R C L S V H L R D D Y L N T E Y F P S Y K M .................................. Protein_Len: 43 Strand: - Start: 2 End: 130 . F N E E T I E Q E M G A V V F V S M C G T M M Y I R K M F H P T N L S Y I F I D D A M ........ aaaaattctcttccgttatttcctgctccataccagctacgacaaagactgacatgcaaccggtcatcatataaattcgtttcataaaatggggagtatttaatgagtaaataaaaatatcatccgccatacgaacta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaaattctcttccgttatttcctgctccataccagctacgacaaagactgacatgcaaccggtcatcatataaattcgtttcataaaatggggagtatttaatgagtaaataaaaatatcatccgccatacgaacta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0688:TF0689|TF0688:TF0689:membrane-associated protein, possible sulfatase family:hypothetical protein:->->:734421..734558 138 aaaaattctcttccgttatttcctgctccataccagctacgacaaagactgacatgcaaccggtcatcatataaattcgtttcataaaatggggagtatttaatgagtaaataaaaatatcatccgccatacgaacta Intra-Species Hit: Count: 1 Min: 1 Max: 138 Len: 138 Subject: tfor_TF0688_TF0689|membrane-associated protein, possible sulfatase family:hypothetical protein|POSITIVE:POSITIVE|[734421,734558]|138 HSP 1 e-value: 7.0E-73 bit: 274.0 Len: 138 Query Start:1 Query End:138 Subject Strand: POSITIVE Subject Start: 1 Subject End: 138 aaaaattctcttccgttatttcctgctccataccagctacgacaaagactgacatgcaaccggtcatcatataaattcgtttcataaaatggggagtatttaatgagtaaataaaaatatcatccgccatacgaacta aaaaattctcttccgttatttcctgctccataccagctacgacaaagactgacatgcaaccggtcatcatataaattcgtttcataaaatggggagtatttaatgagtaaataaaaatatcatccgccatacgaacta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAAUUCUCUUCCGUUAUUUCCUGCUCCAUACCAGCUACGACAAAGACUGACAUGCAACCGGUCAUCAUAUAAAUUCGUUUCAUAAAAUGGGGAGUAUUUAAUGAGUAAAUAAAAAUAUCAUCCGCCAUACGAACUA ..............((..(...(((........)))..)..))...(((((.........)))))..........(((((........(((((((((((((.............))))))..))).)))))))))... (-16.32) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUUCGUAUGGCGGAUGAUAUUUUUAUUUACUCAUUAAAUACUCCCCAUUUUAUGAAACGAAUUUAUAUGAUGACCGGUUGCAUGUCAGUCUUUGUCGUAGCUGGUAUGGAGCAGGAAAUAACGGAAGAGAAUUUUU .(((((..((((..(((((.....)))))..).))).......(((.((((.((((((.....)))))).)))).((.(((.(((..(((.((.......)))))..))).))).)).......)))...)))))... (-27.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 327 68 21 215 169 tfor:TF3125|5end_recombination/repair_protein_3359808..3360038_POSITIVE 324 58 13 178 131 tfor:TF0352|5end_transposase_element_371223..371453_POSITIVE 317 48 8 178 132 tfor:TF1001|5end_penicillin-binding_protein_1A_1054097..1054327_POSITIVE 307 51 13 159 104 tfor:TF1559|5end_glycosyltransferase_1665879..1666109_POSITIVE 306 67 23 227 176 tfor:TF2904|5end_ATP-dependent__helicase,_possible_UvrD/REP_helicase_domain_protein_3098903..3099133_POSITIVE 301 57 11 220 175 tfor:TF2430|5end_hypothetical_protein_2612660..2612890_POSITIVE 300 68 23 223 181 tfor:TF3139|5end_Na+-translocating_NADH-quinone_reductase,_subunit_C_3372581..3372811_POSITIVE 299 68 23 113 62 tfor:TF2204|5end_conserved_hypothetical_protein_2378866..2379096_POSITIVE 294 43 6 120 75 tfor:TF1873|5end_possible_hemolysin_secretion_protein_2026745..2026975_POSITIVE 294 43 6 120 75 tfor:TF1131|5end_membrane-fusion_protein;_possible_hemolysin_secretion_protein_1207611..1207841_POSITIVE 292 68 25 92 42 tfor:TF0248|5end_hypothetical_protein_278249..278479_POSITIVE 288 68 21 153 101 tfor:TF2992|5end_hexapeptide_transferase_family_protein_3209042..3209272_POSITIVE 288 58 11 69 25 tfor:TF2342|5end_glutathione_peroxidase_2505963..2506193_POSITIVE 285 59 12 66 6 tfor:TF1189|5end_outer_membrane_protein,_TonB_dependent_receptor_1270586..1270816_POSITIVE 283 63 22 199 160 tfor:TF2259|5end_transfer_region-related_protein,_TraK_2429577..2429807_POSITIVE 280 66 24 203 167 tfor:TF2926|5end_glycine_hydroxymethyltransferase_3128070..3128300_POSITIVE 279 95 51 54 5 tfor:TF1151|5end_conserved_hypothetical_protein_1232922..1233152_POSITIVE 278 66 21 198 151 tfor:TF1224|5end_hypothetical_protein_1311203..1311433_POSITIVE 277 67 22 70 33 tfor:TF1758|5end_conserved_hypothetical_protein_1894769..1894999_POSITIVE 276 68 22 56 16 tfor:TF2832|5end_possible_proton/sodium:glutamate_symporter_protein_3022660..3022890_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 324 58 13 101 54 tfor:TF0351|3end_hypothetical_protein_371226..371376_POSITIVE 294 43 6 106 61 tfor:TF1872|3end_ABC_transporter,_ATP-binding/permease_component_2026811..2026961_POSITIVE 294 43 6 106 61 tfor:TF1130|3end_ABC_transporter_ATP-binding_protein/permease_component_1207677..1207827_POSITIVE 292 68 25 111 61 tfor:TF0247|3end_conserved_hypothetical_protein_278348..278498_POSITIVE 288 68 21 138 86 tfor:TF2991|3end_conserved_hypothetical_protein_3209107..3209257_POSITIVE 288 58 11 53 9 tfor:TF2341|3end_hypothetical_protein_2506027..2506177_POSITIVE 286 64 21 61 7 tfor:TF0908|3end_integrase_fragment_951024..951174_POSITIVE 278 60 13 46 1 tfor:TF1298|3end_possible_membrane_protein_1381197..1381347_POSITIVE 277 67 22 46 9 tfor:TF1757|3end_chromosome_partitioning_protein_1894825..1894975_POSITIVE 276 44 8 125 81 tfor:TF2654|3end_arginyl-tRNA_synthetase_2824559..2824709_POSITIVE 274 99 51 71 20 tfor:TF0994|3end_maltose_O-acetyltransferase_1049782..1049932_POSITIVE 272 67 22 99 53 tfor:TF2343|3end_ribosome_recycling_factor_2507502..2507652_POSITIVE 271 58 14 116 68 tfor:TF1244|3end_hypothetical_protein_1328813..1328963_POSITIVE 270 65 22 68 10 tfor:TF2937|3end_translation_elongation_factor_Tu_3140502..3140652_POSITIVE 270 96 53 143 101 tfor:TF2768|3end_ferredoxin_oxidoreductase,_beta_subunit_2955169..2955319_POSITIVE 266 100 55 73 35 tfor:TF2034|3end_arabinose-proton_symporter_2194738..2194888_POSITIVE 265 41 8 139 99 tfor:TF0970|3end_2-keto-3-deoxygluconate_kinase_1021540..1021690_POSITIVE 264 57 11 128 82 tfor:TF1994|3end_acyl-CoA_synthetase_2147228..2147378_POSITIVE 262 59 13 57 12 tfor:TF2449|3end_undecaprenyl_diphosphate_synthase_2626541..2626691_POSITIVE 261 42 10 80 55 tfor:TF2099|3end_D-tyrosyl-tRNA(Tyr)_deacylase_2273419..2273569_POSITIVE