Origin IGS: taattatgacatacgactgactatcgtgtcaacctacgctgcatcagcatcaaaaataggttggcaatgagcgtgatcccaatctggatatgtagcgcattggcgttctctccatagaagtatttcaggggaaagacctgcgggtagccgggagaatcattcgctattgctcacaagacaaaagttccatatatttgtaccctgtggtcaacaaataaaaaaaataaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttattttttttatttgttgaccacagggtacaaatatatggaacttttgtcttgtgagcaatagcgaatgattctcccggctacccgcaggtctttcccctgaaatacttctatggagagaacgccaatgcgctacatatccagattgggatcacgctcattgccaacctatttttgatgctgatgcagcgtaggttgacacgatagtcagtcgtatgtcataatta Mask Tandem Repeat Region ================================================ taattatgacatacgactgactatcgtgtcaacctacgctgcatcagcatcaaaaataggttggcaatgagcgtgatcccaatctggatatgtagcgcattggcgttctctccatagaagtatttcaggggaaagacctgcgggtagccgggagaatcattcgctattgctcacaagacaaaagttccatatatttgtaccctgtggtcaacaaataaaaaaaataaa Find is-nt database================================================ Query_seq: TF0668:TF0669|TF0668:TF0669:inorganic pyrophosphatase:hypothetical protein:->->:713296..713523 228 taattatgacatacgactgactatcgtgtcaacctacgctgcatcagcatcaaaaataggttggcaatgagcgtgatcccaatctggatatgtagcgcattggcgttctctccatagaagtatttcaggggaaagacctgcgggtagccgggagaatcattcgctattgctcacaagacaaaagttccatatatttgtaccctgtggtcaacaaataaaaaaaataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0668:TF0669|TF0668:TF0669:inorganic pyrophosphatase:hypothetical protein:->->:713296..713523 228 taattatgacatacgactgactatcgtgtcaacctacgctgcatcagcatcaaaaataggttggcaatgagcgtgatcccaatctggatatgtagcgcattggcgttctctccatagaagtatttcaggggaaagacctgcgggtagccgggagaatcattcgctattgctcacaagacaaaagttccatatatttgtaccctgtggtcaacaaataaaaaaaataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0668:TF0669|TF0668:TF0669:inorganic pyrophosphatase:hypothetical protein:->->:713296..713523 228 taattatgacatacgactgactatcgtgtcaacctacgctgcatcagcatcaaaaataggttggcaatgagcgtgatcccaatctggatatgtagcgcattggcgttctctccatagaagtatttcaggggaaagacctgcgggtagccgggagaatcattcgctattgctcacaagacaaaagttccatatatttgtaccctgtggtcaacaaataaaaaaaataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattatgacatacgactgactatcgtgtcaacctacgctgcatcagcatcaaaaataggttggcaatgagcgtgatcccaatctggatatgtagcgcattggcgttctctccatagaagtatttcaggggaaagacctgcgggtagccgggagaatcattcgctattgctcacaagacaaaagttccatatatttgtaccctgtggtcaacaaataaaaaaaataaa Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 99 End: 215 .................................................................................................. M G V L S I E V F Q G K D L R V A G R I I R Y C S Q D K S S I Y L Y P V V N K ............. Protein_Len: 35 Strand: + Start: 122 End: 226 ......................................................................................................................... M S G E R P A G S R E N H S L L L T R Q K F H I F V P C G Q Q I K K I .. Protein_Len: 48 Strand: - Start: 24 End: 167 ....................... R T L R R Q M L M L F L N A I L T I G I Q I H L A N A N E G Y F Y K L P F V Q P Y G P S D N A M ............................................................. taattatgacatacgactgactatcgtgtcaacctacgctgcatcagcatcaaaaataggttggcaatgagcgtgatcccaatctggatatgtagcgcattggcgttctctccatagaagtatttcaggggaaagacctgcgggtagccgggagaatcattcgctattgctcacaagacaaaagttccatatatttgtaccctgtggtcaacaaataaaaaaaataaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattatgacatacgactgactatcgtgtcaacctacgctgcatcagcatcaaaaataggttggcaatgagcgtgatcccaatctggatatgtagcgcattggcgttctctccatagaagtatttcaggggaaagacctgcgggtagccgggagaatcattcgctattgctcacaagacaaaagttccatatatttgtaccctgtggtcaacaaataaaaaaaataaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0668:TF0669|TF0668:TF0669:inorganic pyrophosphatase:hypothetical protein:->->:713296..713523 228 taattatgacatacgactgactatcgtgtcaacctacgctgcatcagcatcaaaaataggttggcaatgagcgtgatcccaatctggatatgtagcgcattggcgttctctccatagaagtatttcaggggaaagacctgcgggtagccgggagaatcattcgctattgctcacaagacaaaagttccatatatttgtaccctgtggtcaacaaataaaaaaaataaa Intra-Species Hit: Count: 1 Min: 1 Max: 216 Len: 216 Subject: tfor_TF0668_TF0669|inorganic pyrophosphatase:hypothetical protein|POSITIVE:POSITIVE|[713296,713523]|228 HSP 1 e-value: 1.0E-119 bit: 428.0 Len: 216 Query Start:1 Query End:216 Subject Strand: POSITIVE Subject Start: 1 Subject End: 216 taattatgacatacgactgactatcgtgtcaacctacgctgcatcagcatcaaaaataggttggcaatgagcgtgatcccaatctggatatgtagcgcattggcgttctctccatagaagtatttcaggggaaagacctgcgggtagccgggagaatcattcgctattgctcacaagacaaaagttccatatatttgtaccctgtggtcaacaaat............ taattatgacatacgactgactatcgtgtcaacctacgctgcatcagcatcaaaaataggttggcaatgagcgtgatcccaatctggatatgtagcgcattggcgttctctccatagaagtatttcaggggaaagacctgcgggtagccgggagaatcattcgctattgctcacaagacaaaagttccatatatttgtaccctgtggtcaacaaat............ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUAUGACAUACGACUGACUAUCGUGUCAACCUACGCUGCAUCAGCAUCAAAAAUAGGUUGGCAAUGAGCGUGAUCCCAAUCUGGAUAUGUAGCGCAUUGGCGUUCUCUCCAUAGAAGUAUUUCAGGGGAAAGACCUGCGGGUAGCCGGGAGAAUCAUUCGCUAUUGCUCACAAGACAAAAGUUCCAUAUAUUUGUACCCUGUGGUCAACAAAU .................(((((((..((((((((((.((((...))))........))))))))))..((((...((((......)))).(((.(.(((.(((((...(((((............((((.......))))(((....))))))))......))))).))).))))........))))................)))))))...... (-52.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUGUUGACCACAGGGUACAAAUAUAUGGAACUUUUGUCUUGUGAGCAAUAGCGAAUGAUUCUCCCGGCUACCCGCAGGUCUUUCCCCUGAAAUACUUCUAUGGAGAGAACGCCAAUGCGCUACAUAUCCAGAUUGGGAUCACGCUCAUUGCCAACCUAUUUUUGAUGCUGAUGCAGCGUAGGUUGACACGAUAGUCAGUCGUAUGUCAUAAUUA ((((((..(((....))))))))).(((((.((..(((.((((((.(((((((((..(((((((.(.((.((..(((((((((((((...(((....)))...))))))...)))..)))).....)).)).)...))))))))))).)))))((((((((......(((....)))..)))))))).))))..)).)))..))...))))).... (-53.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 383 114 73 97 51 tfor:TF2793|5end_possible_L-lysine_2,3-aminomutase_2977661..2977891_POSITIVE 379 170 121 212 159 tfor:TF0240|5end_conserved_hypothetical_protein;_possible_gycosyltransferase_I_family_protein_270086..270316_POSITIVE 358 180 137 100 54 tfor:TF0470|5end_hypothetical_protein_487253..487483_POSITIVE 355 180 136 225 190 tfor:TF0038|5end_conserved_hypothetical_protein_39907..40137_POSITIVE 349 168 125 80 30 tfor:TF2719|5end_hypothetical_protein_2892701..2892931_POSITIVE 349 175 135 145 102 tfor:TF2517|5end_hypothetical_protein_2695089..2695319_POSITIVE 343 176 139 208 152 tfor:TF2704|5end_ribonuclease_HII_2876242..2876472_POSITIVE 342 79 33 71 33 tfor:TF2893|5end_conserved_hypothetical_protein;_possible_phosphoesterase_3080766..3080996_POSITIVE 341 169 122 84 36 tfor:TF2463|5end_anti-sigma_factor_2641977..2642207_POSITIVE 341 169 126 87 28 tfor:TF0241|5end_UDP-N-acetyl_glucosamine_-2-epimerase_271321..271551_POSITIVE 341 106 63 193 157 tfor:TF0009|5end_transcriptional_regulator_3491..3721_POSITIVE 336 138 95 226 188 tfor:TF2898|5end_conserved_hypothetical_protein_3087061..3087291_POSITIVE 332 180 138 128 83 tfor:TF2653|5end_hypothetical_protein_2822093..2822323_POSITIVE 332 180 138 82 25 tfor:TF0321|5end_glycosyltransferase_347438..347668_POSITIVE 330 98 59 147 107 tfor:TF3108|5end_glutamine-hydrolyzing_carbamoyl-phosphate_synthase,_large_subunit_3338732..3338962_POSITIVE 330 171 134 224 183 tfor:TF2332|5end_signal_peptidase_I_2491694..2491924_POSITIVE 330 170 122 60 14 tfor:TF1201|5end_possible_preprotein_translocase_1283732..1283962_POSITIVE 327 175 133 231 183 tfor:TF2615|5end_conserved_hypothetical_protein_2790569..2790799_POSITIVE 326 62 21 66 25 tfor:TF2446|5end_riboflavin-specific_deaminase_2622370..2622600_POSITIVE 325 159 111 219 162 tfor:TF2877|5end_regulatory_protein_3065601..3065831_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 589 120 73 61 14 tfor:TF1747|3end_hypothetical_protein_1881497..1881647_POSITIVE 379 149 101 131 89 tfor:TF0666|3end_conserved_hypothetical_protein_710945..711095_POSITIVE 349 168 125 110 60 tfor:TF2718|3end_hypothetical_protein_2892811..2892961_POSITIVE 341 169 122 103 55 tfor:TF2462|3end_hypothetical_protein_2642076..2642226_POSITIVE 341 169 126 93 34 tfor:TF0240|3end_conserved_hypothetical_protein;_possible_gycosyltransferase_I_family_protein_271407..271557_POSITIVE 339 180 135 113 68 tfor:TF2745|3end_hypothetical_protein_2927947..2928097_POSITIVE 338 167 123 76 22 tfor:TF1327|3end_L-fucose_permease_1407195..1407345_POSITIVE 333 99 58 48 4 tfor:TF1743|3end_conserved_hypothetical_protein_1877909..1878059_POSITIVE 332 180 138 77 32 tfor:TF2652|3end_hypothetical_protein_2822122..2822272_POSITIVE 332 180 138 82 25 tfor:TF0320|3end_dihydroorotase_347518..347668_POSITIVE 330 163 125 150 101 tfor:TF3143|3end_conserved_hypothetical_protein;_possible_membrane_protein_3379227..3379377_POSITIVE 330 98 59 71 31 tfor:TF3107|3end_hypothetical_protein_3338736..3338886_POSITIVE 318 178 134 57 12 tfor:TF2275|3end_conserved_hypothetical_protein_2438300..2438450_POSITIVE 318 105 64 45 1 tfor:TF2199|3end_outer_membrane_protein,_TonB-linked_2376639..2376789_POSITIVE 318 180 135 147 102 tfor:TF1845|3end_hypothetical_protein_1997005..1997155_POSITIVE 318 180 135 131 86 tfor:TF1659|3end_hypothetical_protein_1775209..1775359_POSITIVE 317 170 123 46 1 tfor:TF1200|3end_conserved_hypothetical_protein_1283798..1283948_POSITIVE 315 117 71 131 86 tfor:TF2653|3end_hypothetical_protein_2822460..2822610_POSITIVE 314 118 71 64 18 tfor:TF1834|3end_3-isopropylmalate_dehydrogenase_1984311..1984461_POSITIVE 312 99 55 58 10 tfor:TF1928|3end_conserved_hypothetical_protein_2076124..2076274_POSITIVE