Origin IGS: taaacgccgatactgtttcatcaggaaacaggcagggaaggcagaccgttcaaggtattcgccttccctgcttttctattttcatggctggaggggtaccgttgtttctcatcaaatctgtgtgaagatttgtctcccgcaaattgtttctgataaatcacagttgtgatttatctcctgcaaattgttttcgcatacggctattta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaatagccgtatgcgaaaacaatttgcaggagataaatcacaactgtgatttatcagaaacaatttgcgggagacaaatcttcacacagatttgatgagaaacaacggtacccctccagccatgaaaatagaaaagcagggaaggcgaataccttgaacggtctgccttccctgcctgtttcctgatgaaacagtatcggcgttta Mask Tandem Repeat Region ================================================ taaacgccgatactgtttcatcaggaaacaggcagggaaggcagaccgttcaaggtattcgccttccctgcttttctattttcatggctggaggggtaccgttgtttctcatcaaatctgtgtgaagatttgtctcccgcaaattgtttctgataaatcacagttgtgatttatctcctgcaaattgttttcgcatacggctattta Find is-nt database================================================ Query_seq: TF0624:TF0625|TF0624:TF0625:Xaa-Pro dipeptidase (aminopeptidase P):hypothetical protein:->->:661076..661282 207 taaacgccgatactgtttcatcaggaaacaggcagggaaggcagaccgttcaaggtattcgccttccctgcttttctattttcatggctggaggggtaccgttgtttctcatcaaatctgtgtgaagatttgtctcccgcaaattgtttctgataaatcacagttgtgatttatctcctgcaaattgttttcgcatacggctattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0624:TF0625|TF0624:TF0625:Xaa-Pro dipeptidase (aminopeptidase P):hypothetical protein:->->:661076..661282 207 taaacgccgatactgtttcatcaggaaacaggcagggaaggcagaccgttcaaggtattcgccttccctgcttttctattttcatggctggaggggtaccgttgtttctcatcaaatctgtgtgaagatttgtctcccgcaaattgtttctgataaatcacagttgtgatttatctcctgcaaattgttttcgcatacggctattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0624:TF0625|TF0624:TF0625:Xaa-Pro dipeptidase (aminopeptidase P):hypothetical protein:->->:661076..661282 207 taaacgccgatactgtttcatcaggaaacaggcagggaaggcagaccgttcaaggtattcgccttccctgcttttctattttcatggctggaggggtaccgttgtttctcatcaaatctgtgtgaagatttgtctcccgcaaattgtttctgataaatcacagttgtgatttatctcctgcaaattgttttcgcatacggctattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaacgccgatactgtttcatcaggaaacaggcagggaaggcagaccgttcaaggtattcgccttccctgcttttctattttcatggctggaggggtaccgttgtttctcatcaaatctgtgtgaagatttgtctcccgcaaattgtttctgataaatcacagttgtgatttatctcctgcaaattgttttcgcatacggctattta Predict ORF larger than 30AA ================================================ Protein_Len: 47 Strand: + Start: 10 End: 150 ......... M L F H Q E T G R E G R P F K V F A F P A F L F S W L E G Y R C F S S N L C E D L S P A N C F ......................................................... Protein_Len: 49 Strand: + Start: 20 End: 166 ................... M R K Q A G K A D R S R Y S P S L L F Y F H G W R G T V V S H Q I C V K I C L P Q I V S D K S Q L ......................................... Protein_Len: 30 Strand: - Start: 115 End: 204 .................................................................................................................. I Q T F I Q R G C I T E S L D C N H N I E Q L N N E C V A M ... Protein_Len: 34 Strand: - Start: 53 End: 154 .................................................... P I R R G Q K E I K M A P P T G N N R M L D T H L N T E R L N N R M ..................................................... Protein_Len: 49 Strand: - Start: 24 End: 170 ....................... S V P L S P L G N L T N A K G A K R N E H S S P Y R Q K E D F R H S S K D G A F Q K Q Y I V T T M ..................................... taaacgccgatactgtttcatcaggaaacaggcagggaaggcagaccgttcaaggtattcgccttccctgcttttctattttcatggctggaggggtaccgttgtttctcatcaaatctgtgtgaagatttgtctcccgcaaattgtttctgataaatcacagttgtgatttatctcctgcaaattgttttcgcatacggctattta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaacgccgatactgtttcatcaggaaacaggcagggaaggcagaccgttcaaggtattcgccttccctgcttttctattttcatggctggaggggtaccgttgtttctcatcaaatctgtgtgaagatttgtctcccgcaaattgtttctgataaatcacagttgtgatttatctcctgcaaattgttttcgcatacggctattta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 93 HP_score: -10.2 Tail_Score: -4.91977 Start: 32 End: 71 Full_Region: TTCATCAGGAAACAG GCAGGGAAGGCAGACC GTTCAA GGTATTCGCCTTCCCTGC TTTTCTATTTTCATG ...............................GCAGGGAAGGCAGACCGTTCAAGGTATTCGCCTTCCCTGC........................................................................................................................................ Find igs database================================================ Query_seq: TF0624:TF0625|TF0624:TF0625:Xaa-Pro dipeptidase (aminopeptidase P):hypothetical protein:->->:661076..661282 207 taaacgccgatactgtttcatcaggaaacaggcagggaaggcagaccgttcaaggtattcgccttccctgcttttctattttcatggctggaggggtaccgttgtttctcatcaaatctgtgtgaagatttgtctcccgcaaattgtttctgataaatcacagttgtgatttatctcctgcaaattgttttcgcatacggctattta Intra-Species Hit: Count: 1 Min: 1 Max: 207 Len: 207 Subject: tfor_TF0624_TF0625|Xaa-Pro dipeptidase (aminopeptidase P):hypothetical protein|POSITIVE:POSITIVE|[661076,661282]|207 HSP 1 e-value: 1.0E-114 bit: 410.0 Len: 207 Query Start:1 Query End:207 Subject Strand: POSITIVE Subject Start: 1 Subject End: 207 taaacgccgatactgtttcatcaggaaacaggcagggaaggcagaccgttcaaggtattcgccttccctgcttttctattttcatggctggaggggtaccgttgtttctcatcaaatctgtgtgaagatttgtctcccgcaaattgtttctgataaatcacagttgtgatttatctcctgcaaattgttttcgcatacggctattta taaacgccgatactgtttcatcaggaaacaggcagggaaggcagaccgttcaaggtattcgccttccctgcttttctattttcatggctggaggggtaccgttgtttctcatcaaatctgtgtgaagatttgtctcccgcaaattgtttctgataaatcacagttgtgatttatctcctgcaaattgttttcgcatacggctattta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACGCCGAUACUGUUUCAUCAGGAAACAGGCAGGGAAGGCAGACCGUUCAAGGUAUUCGCCUUCCCUGCUUUUCUAUUUUCAUGGCUGGAGGGGUACCGUUGUUUCUCAUCAAAUCUGUGUGAAGAUUUGUCUCCCGCAAAUUGUUUCUGAUAAAUCACAGUUGUGAUUUAUCUCCUGCAAAUUGUUUUCGCAUACGGCUAUUUA .....((((...(((((((.....)))))))(((((((((((..(((......)))....)))))))))))...............((.(((((((.((....))..)))..(((((((......))))))).)))).((((.((((....((((((((((....))))))))))....)))).))))....))...))))...... (-72.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUAGCCGUAUGCGAAAACAAUUUGCAGGAGAUAAAUCACAACUGUGAUUUAUCAGAAACAAUUUGCGGGAGACAAAUCUUCACACAGAUUUGAUGAGAAACAACGGUACCCCUCCAGCCAUGAAAAUAGAAAAGCAGGGAAGGCGAAUACCUUGAACGGUCUGCCUUCCCUGCCUGUUUCCUGAUGAAACAGUAUCGGCGUUUA ......(((((.((((((.....))))))...((((((((((....))))))))))......((((((.((((((....))))).).))))))............))))).........(((..............((((((((((((...(((......))).))))))))))))(((((((.....)))))))....)))..... (-68.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 461 140 93 227 181 tfor:TF0686|5end_hypothetical_protein_732199..732429_POSITIVE 398 49 5 53 18 tfor:TF0231|5end_conserved_hypothetical_protein_257804..258034_POSITIVE 376 125 81 77 23 tfor:TF2642|5end_acetylornithine_deacetylase_2810677..2810907_POSITIVE 368 76 31 67 7 tfor:TF1465|5end_modulator_of_DNA_gyrase_1554148..1554378_POSITIVE 367 76 33 89 37 tfor:TF2120|5end_holliday_junction_endonuclease_2292108..2292338_POSITIVE 361 77 31 167 116 tfor:TF0480|5end_ornithine_carbamoyltransferase_498550..498780_POSITIVE 356 70 27 183 140 tfor:TF1651|5end_integrase_1761296..1761526_POSITIVE 351 67 21 165 122 tfor:TF2747|5end_heat_shock_protein,_HSP90_family_2928279..2928509_POSITIVE 351 84 42 182 137 tfor:TF2099|5end_D-tyrosyl-tRNA(Tyr)_deacylase_2272896..2273126_POSITIVE 348 70 24 135 82 tfor:TF2785|5end_RNA_polymerase_sigma_factor,_ECF_subfamily_(sigma-70)_2969277..2969507_POSITIVE 346 95 51 86 44 tfor:TF2932|5end_capsule_biosynthesis_protein_3135514..3135744_POSITIVE 345 99 51 125 80 tfor:TF0583|5end_possible_gliding_motility-related_protein_613189..613419_POSITIVE 340 90 41 164 97 tfor:TF3022|5end_chorismate_synthase_3246136..3246366_POSITIVE 339 140 95 64 2 tfor:TF3119|5end_conserved_hypothetical_protein_3352723..3352953_POSITIVE 339 71 32 184 129 tfor:TF1850|5end_Na+/H+-exchanging_protein_1998542..1998772_POSITIVE 338 77 32 231 173 tfor:TF2674|5end_conserved_hypothetical_protein;_possible_phosphoesterase_2848176..2848406_POSITIVE 337 50 8 182 147 tfor:TF1921|5end_hypothetical_protein_2070322..2070552_POSITIVE 335 76 33 174 140 tfor:TF1631|5end_hypothetical_protein_1743091..1743321_POSITIVE 335 200 163 178 133 tfor:TF0971|5end_conserved_hypothetical_protein_1021355..1021585_POSITIVE 333 90 46 174 122 tfor:TF2261|5end_transfer_region-related_protein,_TraM_2430496..2430726_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 653 177 131 49 3 tfor:TF0684|3end_conserved_hypothetical_protein_730564..730714_POSITIVE 441 77 31 68 15 tfor:TF0624|3end_Xaa-Pro_dipeptidase_(aminopeptidase_P)_661015..661165_POSITIVE 367 76 33 89 37 tfor:TF2119|3end_conserved_hypothetical_protein_2292188..2292338_POSITIVE 361 100 51 58 12 tfor:TF2340|3end_hypothetical_protein_2500915..2501065_POSITIVE 358 70 22 54 5 tfor:TF0230|3end_hydrolase,_haloacid_dehalogenase-like_family_257862..258012_POSITIVE 351 80 34 78 17 tfor:TF2850|3end_endoribonuclease_L-PSP_3038871..3039021_POSITIVE 351 67 21 52 9 tfor:TF2746|3end_hypothetical_protein_2928246..2928396_POSITIVE 351 84 42 148 103 tfor:TF2098|3end_excinuclease_ABC,_subunit_C_2272942..2273092_POSITIVE 348 70 24 75 22 tfor:TF2783|3end_aminotransferase,_PatB_family_2969297..2969447_POSITIVE 346 95 51 76 34 tfor:TF2931|3end_3-deoxy-D-manno-2-octulosonic_acid_(Kdo_transferase)_3135584..3135734_POSITIVE 345 99 51 119 74 tfor:TF0582|3end_hemolysin-related_protein,_with_CBS_domains;_possible_gliding-motility_related_protein_613263..613413_POSITIVE 340 90 41 142 75 tfor:TF3021|3end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_3246194..3246344_POSITIVE 339 140 95 85 23 tfor:TF3118|3end_conserved_hypothetical_protein_3352824..3352974_POSITIVE 335 99 51 69 5 tfor:TF1221|3end_conserved_hypothetical_protein_1308363..1308513_POSITIVE 332 48 3 121 78 tfor:TF2991|3end_conserved_hypothetical_protein_3209107..3209257_POSITIVE 332 77 31 62 9 tfor:TF0382|3end_transcription_termination_factor_405228..405378_POSITIVE 329 75 32 67 30 tfor:TF2482|3end_hypothetical_protein_2659185..2659335_POSITIVE 325 130 85 66 14 tfor:TF1797|3end_SNF_family_transporter_1938571..1938721_POSITIVE 324 125 84 52 1 tfor:TF2641|3end_riboflavin_kinase/FAD_synthase_2810732..2810882_POSITIVE 322 48 6 148 109 tfor:TF1346|3end_hypothetical_protein_1426214..1426364_POSITIVE