Origin IGS: gcgtagcgaagtcgtgtgaagcgcacgaagccttgactttttggttacttttgggtcaagccaaaagtgacggagaaaaagtgacggaaaaattatgtcaggctttcagcccttgtgccttctttgggtgttgaattgttgagttgttgaattgttgactgaaaactgaccgctaataagaattacgaactgaatactgatggctgaatgctaaacaaaaaacatctaaacaactaaatatcctccgatcacaccaacagaagtttagagaagagccgataagtttagacaaaaggagaaaatcaaacagccaaaaatggggataccaagacaaaaaacaccaagtattagggattgcccataaccacagaacggccgtcctttatatttacaaaactttatttaacaaactaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttagtttgttaaataaagttttgtaaatataaaggacggccgttctgtggttatgggcaatccctaatacttggtgttttttgtcttggtatccccatttttggctgtttgattttctccttttgtctaaacttatcggctcttctctaaacttctgttggtgtgatcggaggatatttagttgtttagatgttttttgtttagcattcagccatcagtattcagttcgtaattcttattagcggtcagttttcagtcaacaattcaacaactcaacaattcaacacccaaagaaggcacaagggctgaaagcctgacataatttttccgtcactttttctccgtcacttttggcttgacccaaaagtaaccaaaaagtcaaggcttcgtgcgcttcacacgacttcgctacgc Mask Tandem Repeat Region ================================================ gcgtagcgaagtcgtgtgaagcgcacgaagccttgactttttggttacttttgggtcaagccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttatgtcaggctttcagcccttgtgccttctttgggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnctgaaaactgaccgctaataagaattacgaactgaatactgatggctgaatgctaaacaaaaaacatctaaacaactaaatatcctccgatcacaccaacagaagtttagagaagagccgataagtttagacaaaaggagaaaatcaaacagccaaaaatggggataccaagacaaaaaacaccaagtattagggattgcccataaccacagaacggccgtcctttatatttacaaaactttatttaacaaactaa Find is-nt database================================================ Query_seq: TF0598:TF0599|TF0598:TF0599:hypothetical protein:hypothetical protein:->->:629561..629974 414 gcgtagcgaagtcgtgtgaagcgcacgaagccttgactttttggttacttttgggtcaagccaaaagtgacggagaaaaagtgacggaaaaattatgtcaggctttcagcccttgtgccttctttgggtgttgaattgttgagttgttgaattgttgactgaaaactgaccgctaataagaattacgaactgaatactgatggctgaatgctaaacaaaaaacatctaaacaactaaatatcctccgatcacaccaacagaagtttagagaagagccgataagtttagacaaaaggagaaaatcaaacagccaaaaatggggataccaagacaaaaaacaccaagtattagggattgcccataaccacagaacggccgtcctttatatttacaaaactttatttaacaaactaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0598:TF0599|TF0598:TF0599:hypothetical protein:hypothetical protein:->->:629561..629974 414 gcgtagcgaagtcgtgtgaagcgcacgaagccttgactttttggttacttttgggtcaagccaaaagtgacggagaaaaagtgacggaaaaattatgtcaggctttcagcccttgtgccttctttgggtgttgaattgttgagttgttgaattgttgactgaaaactgaccgctaataagaattacgaactgaatactgatggctgaatgctaaacaaaaaacatctaaacaactaaatatcctccgatcacaccaacagaagtttagagaagagccgataagtttagacaaaaggagaaaatcaaacagccaaaaatggggataccaagacaaaaaacaccaagtattagggattgcccataaccacagaacggccgtcctttatatttacaaaactttatttaacaaactaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0598:TF0599|TF0598:TF0599:hypothetical protein:hypothetical protein:->->:629561..629974 414 gcgtagcgaagtcgtgtgaagcgcacgaagccttgactttttggttacttttgggtcaagccaaaagtgacggagaaaaagtgacggaaaaattatgtcaggctttcagcccttgtgccttctttgggtgttgaattgttgagttgttgaattgttgactgaaaactgaccgctaataagaattacgaactgaatactgatggctgaatgctaaacaaaaaacatctaaacaactaaatatcctccgatcacaccaacagaagtttagagaagagccgataagtttagacaaaaggagaaaatcaaacagccaaaaatggggataccaagacaaaaaacaccaagtattagggattgcccataaccacagaacggccgtcctttatatttacaaaactttatttaacaaactaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gcgtagcgaagtcgtgtgaagcgcacgaagccttgactttttggttacttttgggtcaagccaaaagtgacggagaaaaagtgacggaaaaattatgtcaggctttcagcccttgtgccttctttgggtgttgaattgttgagttgttgaattgttgactgaaaactgaccgctaataagaattacgaactgaatactgatggctgaatgctaaacaaaaaacatctaaacaactaaatatcctccgatcacaccaacagaagtttagagaagagccgataagtttagacaaaaggagaaaatcaaacagccaaaaatggggataccaagacaaaaaacaccaagtattagggattgcccataaccacagaacggccgtcctttatatttacaaaactttatttaacaaactaa Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 33 End: 131 ................................ M T F W L L L G Q A K S D G E K V T E K L C Q A F S P C A F F G C ........................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 204 End: 413 ........................................................................................................................................................................................................... M N A K Q K T S K Q L N I L R S H Q Q K F R E E P I S L D K R R K S N S Q K W G Y Q D K K H Q V L G I A H N H R T A V L . Protein_Len: 45 Strand: - Start: 268 End: 402 ........................................................................................................................................................................................................................................................................... L L A S L N L C F S F I L C G F I P I G L C F V G L I L S Q G Y G C F P R G K Y K C F K M ............ Protein_Len: 60 Strand: - Start: 176 End: 370 ............................................................................................................................................................................... S Y Q H S F A L C F V D L C S F I R R D C W C F N L S S G I L K S L L L F D F L W F H P Y W S L F C W T N P I A W L W M ............................................ Protein_Len: 31 Strand: - Start: 109 End: 201 ............................................................................................................ G K H R R Q T N F Q Q T T S N N V S F Q G S I L I V F Q I S M ..................................................................................................................................................................................................................... Protein_Len: 32 Strand: - Start: 1 End: 96 R L S T T H L A R L R S K K T V K P D L W F H R L F L S P F I M .............................................................................................................................................................................................................................................................................................................................. gcgtagcgaagtcgtgtgaagcgcacgaagccttgactttttggttacttttgggtcaagccaaaagtgacggagaaaaagtgacggaaaaattatgtcaggctttcagcccttgtgccttctttgggtgttgaattgttgagttgttgaattgttgactgaaaactgaccgctaataagaattacgaactgaatactgatggctgaatgctaaacaaaaaacatctaaacaactaaatatcctccgatcacaccaacagaagtttagagaagagccgataagtttagacaaaaggagaaaatcaaacagccaaaaatggggataccaagacaaaaaacaccaagtattagggattgcccataaccacagaacggccgtcctttatatttacaaaactttatttaacaaactaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gcgtagcgaagtcgtgtgaagcgcacgaagccttgactttttggttacttttgggtcaagccaaaagtgacggagaaaaagtgacggaaaaattatgtcaggctttcagcccttgtgccttctttgggtgttgaattgttgagttgttgaattgttgactgaaaactgaccgctaataagaattacgaactgaatactgatggctgaatgctaaacaaaaaacatctaaacaactaaatatcctccgatcacaccaacagaagtttagagaagagccgataagtttagacaaaaggagaaaatcaaacagccaaaaatggggataccaagacaaaaaacaccaagtattagggattgcccataaccacagaacggccgtcctttatatttacaaaactttatttaacaaactaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0598:TF0599|TF0598:TF0599:hypothetical protein:hypothetical protein:->->:629561..629974 414 gcgtagcgaagtcgtgtgaagcgcacgaagccttgactttttggttacttttgggtcaagccaaaagtgacggagaaaaagtgacggaaaaattatgtcaggctttcagcccttgtgccttctttgggtgttgaattgttgagttgttgaattgttgactgaaaactgaccgctaataagaattacgaactgaatactgatggctgaatgctaaacaaaaaacatctaaacaactaaatatcctccgatcacaccaacagaagtttagagaagagccgataagtttagacaaaaggagaaaatcaaacagccaaaaatggggataccaagacaaaaaacaccaagtattagggattgcccataaccacagaacggccgtcctttatatttacaaaactttatttaacaaactaa Intra-Species Hit: Count: 3 Min: 1 Max: 414 Len: 414 Subject: tfor_TF0598_TF0599|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[629561,629974]|414 HSP 1 e-value: 0.0 bit: 821.0 Len: 414 Query Start:1 Query End:414 Subject Strand: POSITIVE Subject Start: 1 Subject End: 414 gcgtagcgaagtcgtgtgaagcgcacgaagccttgactttttggttacttttgggtcaagccaaaagtgacggagaaaaagtgacggaaaaattatgtcaggctttcagcccttgtgccttctttgggtgttgaattgttgagttgttgaattgttgactgaaaactgaccgctaataagaattacgaactgaatactgatggctgaatgctaaacaaaaaacatctaaacaactaaatatcctccgatcacaccaacagaagtttagagaagagccgataagtttagacaaaaggagaaaatcaaacagccaaaaatggggataccaagacaaaaaacaccaagtattagggattgcccataaccacagaacggccgtcctttatatttacaaaactttatttaacaaactaa gcgtagcgaagtcgtgtgaagcgcacgaagccttgactttttggttacttttgggtcaagccaaaagtgacggagaaaaagtgacggaaaaattatgtcaggctttcagcccttgtgccttctttgggtgttgaattgttgagttgttgaattgttgactgaaaactgaccgctaataagaattacgaactgaatactgatggctgaatgctaaacaaaaaacatctaaacaactaaatatcctccgatcacaccaacagaagtttagagaagagccgataagtttagacaaaaggagaaaatcaaacagccaaaaatggggataccaagacaaaaaacaccaagtattagggattgcccataaccacagaacggccgtcctttatatttacaaaactttatttaacaaactaa Subject: tfor_TF0498_TF0499|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[526016,526396]|381 HSP 1 e-value: 1.0E-36 bit: 155.0 Len: 114 Query Start:271 Query End:384 Subject Strand: NEGATIVE Subject Start: 37 Subject End: 150 ..............................................................................................................................................................................................................................................................................aagagccgataagtttagacaaaaggagaaaatcaaacagccaaaaatggggataccaagacaaaaaacaccaagtattagggattgcccataaccacagaacggccgtccttt.............................. ..............................................................................................................................................................................................................................................................................aagagccgataagttcagacaaaaagagaaaatcaaacagccaaaaatagggataacaagacaaaaaataccaattattagggattgtccataaccacataacgaccgtccttt.............................. Subject: tfor_TF2998_TF2999|surface antigen BspA:conserved hypothetical protein|POSITIVE:POSITIVE|[3215212,3215705]|494 HSP 1 e-value: 1.0E-15 bit: 85.7 Len: 55 Query Start:20 Query End:74 Subject Strand: NEGATIVE Subject Start: 54 Subject End: 108 ...................agcgcacgaagccttgactttttggttacttttgggtcaagccaaaagtgacgga.................................................................................................................................................................................................................................................................................................................................................... ...................agcgcacgaagccttgacctttgggttacttttgggtcaagccaaaagtaacgga.................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 6.0E-8 bit: 60.0 Len: 30 Query Start:90 Query End:119 Subject Strand: NEGATIVE Subject Start: 378 Subject End: 407 .........................................................................................aaattatgtcaggctttcagcccttgtgcc....................................................................................................................................................................................................................................................................................................... .........................................................................................aaattatgtcaggctttcagcccttgtgcc....................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCGUAGCGAAGUCGUGUGAAGCGCACGAAGCCUUGACUUUUUGGUUACUUUUGGGUCAAGCCAAAAGUGACGGAGAAAAAGUGACGGAAAAAUUAUGUCAGGCUUUCAGCCCUUGUGCCUUCUUUGGGUGUUGAAUUGUUGAGUUGUUGAAUUGUUGACUGAAAACUGACCGCUAAUAAGAAUUACGAACUGAAUACUGAUGGCUGAAUGCUAAACAAAAAACAUCUAAACAACUAAAUAUCCUCCGAUCACACCAACAGAAGUUUAGAGAAGAGCCGAUAAGUUUAGACAAAAGGAGAAAAUCAAACAGCCAAAAAUGGGGAUACCAAGACAAAAAACACCAAGUAUUAGGGAUUGCCCAUAACCACAGAACGGCCGUCCUUUAUAUUUACAAAACUUUAUUUAACAAACUAA .((..((....(((((((...))))))).))..))..(((((.(((((((((((......))))))))))).))))).((((((.(.........((((.(((.....)))..(((.(((..(((((.((((((..((((.(((((((....((((...((.........((.((((....)))))).............((((.....))))..))...))))....))))))).))))..((((..((.........)).(((((((..............)))))))....))))....)).)))).)))))...))).)))....)))).........((((((.((((((.(((..............))).)))))).))))))......).)))))).......... (-91.68) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAGUUUGUUAAAUAAAGUUUUGUAAAUAUAAAGGACGGCCGUUCUGUGGUUAUGGGCAAUCCCUAAUACUUGGUGUUUUUUGUCUUGGUAUCCCCAUUUUUGGCUGUUUGAUUUUCUCCUUUUGUCUAAACUUAUCGGCUCUUCUCUAAACUUCUGUUGGUGUGAUCGGAGGAUAUUUAGUUGUUUAGAUGUUUUUUGUUUAGCAUUCAGCCAUCAGUAUUCAGUUCGUAAUUCUUAUUAGCGGUCAGUUUUCAGUCAACAAUUCAACAACUCAACAAUUCAACACCCAAAGAAGGCACAAGGGCUGAAAGCCUGACAUAAUUUUUCCGUCACUUUUUCUCCGUCACUUUUGGCUUGACCCAAAAGUAACCAAAAAGUCAAGGCUUCGUGCGCUUCACACGACUUCGCUACGC ...(..((((((((((((...(.((((((....(((((((((....((((.(((((((((.(((........)).)....))))))..)))...))))...))))))))).......(((((..(((......(((((((................))))))).)))..)))))........)))))).)....))))))))))))..)..............................(((((((((((((((((((.....(((..........................)))........))))))))).))))).........((...(((((((....((.((((((((......)))))))).)).)))))))...))..(((((.......)))))...)))))... (-87.68) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 935 60 11 84 35 tfor:TF0469|5end_hypothetical_protein_486960..487190_POSITIVE 935 60 11 53 4 tfor:TF0468|5end_hypothetical_protein_486929..487159_POSITIVE 922 70 21 161 112 tfor:TF1578|5end_hypothetical_protein_1686187..1686417_POSITIVE 922 70 21 145 96 tfor:TF1168|5end_hypothetical_protein_1248755..1248985_POSITIVE 899 70 21 74 25 tfor:TF0685|5end_hypothetical_protein_730852..731082_POSITIVE 870 70 21 145 96 tfor:TF1588|5end_hypothetical_protein_1702268..1702498_POSITIVE 847 70 21 161 112 tfor:TF2997|5end_hypothetical_protein_3211408..3211638_POSITIVE 847 70 21 135 86 tfor:TF2996|5end_hypothetical_protein_3210802..3211032_POSITIVE 602 158 114 172 129 tfor:TF0347|5end_possible_serine_protease_367542..367772_POSITIVE 600 89 41 56 1 tfor:TF2921|5end_hypothetical_protein_3118498..3118728_POSITIVE 557 169 124 157 111 tfor:TF1175|5end_membrane-fusion_protein_1258140..1258370_POSITIVE 551 158 114 142 99 tfor:TF0367|5end_eukaryotic-like__metalloproteinase_384007..384237_POSITIVE 551 158 114 142 99 tfor:TF0364|5end_ysyl_endopeptidase_380395..380625_POSITIVE 546 128 81 230 162 tfor:TF2999|5end_conserved_hypothetical_protein_3215566..3215796_POSITIVE 545 169 121 175 128 tfor:TF0359|5end_conserved_hypothetical_protein_376943..377173_POSITIVE 513 158 114 182 131 tfor:TF1173|5end_ABC_transporter,_permease_component_1255444..1255674_POSITIVE 486 160 114 177 122 tfor:TF0351|5end_hypothetical_protein_370922..371152_POSITIVE 482 150 101 183 126 tfor:TF0725|5end_O-acetylhomoserine_(thiol)-lyase_776556..776786_POSITIVE 480 169 122 58 1 tfor:TF0350|5end_hypothetical_protein_370794..371024_POSITIVE 459 160 114 179 131 tfor:TF1172|5end_ABC_transporter,_permease_component_1252806..1253036_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 922 70 21 128 79 tfor:TF1577|3end_hypothetical_protein_1686234..1686384_POSITIVE 875 70 21 149 100 tfor:TF2668|3end_hypothetical_protein_2842733..2842883_POSITIVE 875 70 21 121 72 tfor:TF2667|3end_hypothetical_protein_2842705..2842855_POSITIVE 875 70 21 121 72 tfor:TF2665|3end_hypothetical_protein_2842235..2842385_POSITIVE 875 70 21 149 100 tfor:TF2666|3end_hypothetical_protein_2842263..2842413_POSITIVE 875 70 21 121 72 tfor:TF2664|3end_hypothetical_protein_2841730..2841880_POSITIVE 859 70 23 72 25 tfor:TF2914|3end_hypothetical_protein_3109643..3109793_POSITIVE 847 70 21 102 53 tfor:TF2994|3end_hypothetical_protein_3210849..3210999_POSITIVE 769 77 31 49 3 tfor:TF2639|3end_hypothetical_protein_2809333..2809483_POSITIVE 600 380 331 50 1 tfor:TF0498|3end_hypothetical_protein_525955..526105_POSITIVE 577 128 91 110 73 tfor:TF2816|3end_hypothetical_protein_3007184..3007334_POSITIVE 564 76 37 39 1 tfor:TF2998|3end_surface_antigen_BspA_3215151..3215301_POSITIVE 541 76 42 35 1 tfor:TF1576|3end_hypothetical_protein_1686135..1686285_POSITIVE 459 160 114 92 44 tfor:TF1170|3end_conserved_hypothetical_protein_1252799..1252949_POSITIVE 431 139 91 91 17 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 404 119 71 102 52 tfor:TF3106|3end_hypothetical_protein_3338608..3338758_POSITIVE 403 134 98 125 88 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 400 129 82 67 24 tfor:TF0340|3end_hypothetical_protein_364257..364407_POSITIVE 383 149 101 72 15 tfor:TF2260|3end_transfer_region-related_protein,_TraL_2430592..2430742_POSITIVE 381 138 93 126 83 tfor:TF1405|3end_conserved_hypothetical_protein_1481819..1481969_POSITIVE