Origin IGS: taaccataagctgcgaaggaaaggggcgggctccgtgtccgccccccctttgtcattccgaacgtagtgaggaatctcgcgaggaaaaagaaggagattcttcgcttcgccctgaatgacaaattaatggttaattgtt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aacaattaaccattaatttgtcattcagggcgaagcgaagaatctccttctttttcctcgcgagattcctcactacgttcggaatgacaaagggggggcggacacggagcccgcccctttccttcgcagcttatggtta Mask Tandem Repeat Region ================================================ taaccataagctgcgaaggaaaggggcgggctccgtgtccgccccccctttgtcattccgaacgtagtgaggaatctcgcgaggaaaaagaaggagattcttcgcttcgccctgaatgacaaattaatggttaattgtt Find is-nt database================================================ Query_seq: TF0541:TF0542|TF0541:TF0542:conserved hypothetical protein:hypothetical protein:->->:571919..572057 139 taaccataagctgcgaaggaaaggggcgggctccgtgtccgccccccctttgtcattccgaacgtagtgaggaatctcgcgaggaaaaagaaggagattcttcgcttcgccctgaatgacaaattaatggttaattgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0541:TF0542|TF0541:TF0542:conserved hypothetical protein:hypothetical protein:->->:571919..572057 139 taaccataagctgcgaaggaaaggggcgggctccgtgtccgccccccctttgtcattccgaacgtagtgaggaatctcgcgaggaaaaagaaggagattcttcgcttcgccctgaatgacaaattaatggttaattgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0541:TF0542|TF0541:TF0542:conserved hypothetical protein:hypothetical protein:->->:571919..572057 139 taaccataagctgcgaaggaaaggggcgggctccgtgtccgccccccctttgtcattccgaacgtagtgaggaatctcgcgaggaaaaagaaggagattcttcgcttcgccctgaatgacaaattaatggttaattgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaccataagctgcgaaggaaaggggcgggctccgtgtccgccccccctttgtcattccgaacgtagtgaggaatctcgcgaggaaaaagaaggagattcttcgcttcgccctgaatgacaaattaatggttaattgtt Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 6 End: 116 ..... M S C E G K G R A P C P P P L C H S E R S E E S R E E K E G D S S L R P E ....................... Protein_Len: 42 Strand: - Start: 3 End: 128 .. G Y A A F S L P P S R T R G G K T M G F T T L F R A L F F F S I R R K A R F S L N M ........... Protein_Len: 39 Strand: - Start: 1 End: 117 L W L S R L F P A P E T D A G G K D N R V Y H P I E R P F L L L N K A E G Q M ...................... taaccataagctgcgaaggaaaggggcgggctccgtgtccgccccccctttgtcattccgaacgtagtgaggaatctcgcgaggaaaaagaaggagattcttcgcttcgccctgaatgacaaattaatggttaattgtt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaccataagctgcgaaggaaaggggcgggctccgtgtccgccccccctttgtcattccgaacgtagtgaggaatctcgcgaggaaaaagaaggagattcttcgcttcgccctgaatgacaaattaatggttaattgtt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 85 HP_score: -11.9 Tail_Score: -3.90856 Start: 23 End: 45 Full_Region: cggaatgacaaaggg ggggcggac acgga gcccgcccc tttccttcgcagctt ......................ggggcggacacggagcccgcccc.............................................................................................. Find igs database================================================ Query_seq: TF0541:TF0542|TF0541:TF0542:conserved hypothetical protein:hypothetical protein:->->:571919..572057 139 taaccataagctgcgaaggaaaggggcgggctccgtgtccgccccccctttgtcattccgaacgtagtgaggaatctcgcgaggaaaaagaaggagattcttcgcttcgccctgaatgacaaattaatggttaattgtt Intra-Species Hit: Count: 1 Min: 1 Max: 139 Len: 139 Subject: tfor_TF0541_TF0542|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[571919,572057]|139 HSP 1 e-value: 6.0E-61 bit: 234.0 Len: 139 Query Start:1 Query End:139 Subject Strand: POSITIVE Subject Start: 1 Subject End: 139 taaccataagctgcgaaggaaaggggcgggctccgtgtccgnnnnnnntttgtcattccgaacgtagtgaggaatctcgcgaggaaaaagaaggagattcttcgcttcgccctgaatgacaaattaatggttaattgtt taaccataagctgcgaaggaaaggggcgggctccgtgtccgccccccctttgtcattccgaacgtagtgaggaatctcgcgaggaaaaagaaggagattcttcgcttcgccctgaatgacaaattaatggttaattgtt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACCAUAAGCUGCGAAGGAAAGGGGCGGGCUCCGUGUCCGCCCCCCCUUUGUCAUUCCGAACGUAGUGAGGAAUCUCGCGAGGAAAAAGAAGGAGAUUCUUCGCUUCGCCCUGAAUGACAAAUUAAUGGUUAAUUGUU (((((((.......(((((...(((((((((.....))))))))).)))))(((((((.(..((.(((((((((((((.(............)))))))))))))).))..).)))))))......)))))))...... (-61.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACAAUUAACCAUUAAUUUGUCAUUCAGGGCGAAGCGAAGAAUCUCCUUCUUUUUCCUCGCGAGAUUCCUCACUACGUUCGGAAUGACAAAGGGGGGGCGGACACGGAGCCCGCCCCUUUCCUUCGCAGCUUAUGGUUA ......(((((((...((((((((((.(((((.((.((.((((((((............).))))))).)).)).))))).))))))))))((((((((((.(.....).))))))))..))..........))))))) (-53.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 806 120 71 165 117 tfor:TF0017|5end_hypothetical_protein_10504..10734_POSITIVE 757 100 51 174 124 tfor:TF1579|5end_hypothetical_protein_1686889..1687119_POSITIVE 755 120 71 89 39 tfor:TF2994|5end_hypothetical_protein_3210605..3210835_POSITIVE 755 80 32 189 144 tfor:TF2922|5end_surface_antigen_BspA_3119122..3119352_POSITIVE 733 110 61 117 68 tfor:TF2653|5end_hypothetical_protein_2822093..2822323_POSITIVE 731 120 71 171 122 tfor:TF2668|5end_hypothetical_protein_2842471..2842701_POSITIVE 731 120 71 95 46 tfor:TF2667|5end_hypothetical_protein_2842395..2842625_POSITIVE 714 90 41 224 169 tfor:TF1593|5end_hypothetical_protein_1708859..1709089_POSITIVE 705 100 51 214 160 tfor:TF1583|5end_conserved_hypothetical_protein;_possible_endonuclease_1689445..1689675_POSITIVE 701 88 41 231 184 tfor:TF1591|5end_surface_antigen_BspA_1706387..1706617_POSITIVE 693 120 71 99 49 tfor:TF0470|5end_hypothetical_protein_487253..487483_POSITIVE 681 120 71 208 159 tfor:TF2669|5end_possible_Fe-S_oxidoreductase_2842971..2843201_POSITIVE 681 120 71 171 122 tfor:TF2665|5end_hypothetical_protein_2841973..2842203_POSITIVE 638 100 51 156 102 tfor:TF1585|5end_hypothetical_protein_1690369..1690599_POSITIVE 638 100 51 156 102 tfor:TF1584|5end_hypothetical_protein_1690245..1690475_POSITIVE 638 100 51 156 102 tfor:TF1582|5end_hypothetical_protein_1690121..1690351_POSITIVE 589 97 51 67 17 tfor:TF1581|5end_hypothetical_protein_1689199..1689429_POSITIVE 577 124 83 45 4 tfor:TF0018|5end_hypothetical_protein_11250..11480_POSITIVE 567 50 1 210 160 tfor:TF0538|5end_hypothetical_protein_566891..567121_POSITIVE 567 50 1 203 153 tfor:TF0539|5end_hypothetical_protein_566884..567114_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 761 100 51 89 35 tfor:TF1582|3end_hypothetical_protein_1690506..1690656_POSITIVE 733 110 61 66 17 tfor:TF2652|3end_hypothetical_protein_2822122..2822272_POSITIVE 729 100 51 142 94 tfor:TF0016|3end_hypothetical_protein_10581..10731_POSITIVE 715 99 51 151 102 tfor:TF2350|3end_hypothetical_protein_2515364..2515514_POSITIVE 694 110 61 53 5 tfor:TF2997|3end_hypothetical_protein_3211664..3211814_POSITIVE 682 86 45 150 111 tfor:TF2173|3end_hypothetical_protein_2346063..2346213_POSITIVE 682 86 45 150 111 tfor:TF0346|3end_hypothetical_protein_367316..367466_POSITIVE 670 85 41 55 1 tfor:TF2913|3end_conserved_hypothetical_protein_3109117..3109267_POSITIVE 663 86 46 57 17 tfor:TF2640|3end_hypothetical_protein_2809533..2809683_POSITIVE 652 110 61 146 96 tfor:TF0017|3end_hypothetical_protein_11231..11381_POSITIVE 644 86 47 150 111 tfor:TF2166|3end_hypothetical_protein_2342362..2342512_POSITIVE 643 86 45 150 111 tfor:TF0366|3end_hypothetical_protein_383811..383961_POSITIVE 643 86 45 150 111 tfor:TF0362|3end_hypothetical_protein_380199..380349_POSITIVE 624 120 71 74 20 tfor:TF1583|3end_conserved_hypothetical_protein;_possible_endonuclease_1690223..1690373_POSITIVE 613 70 22 151 96 tfor:TF1584|3end_hypothetical_protein_1690603..1690753_POSITIVE 612 95 51 151 102 tfor:TF1581|3end_hypothetical_protein_1689467..1689617_POSITIVE 579 130 83 52 2 tfor:TF1578|3end_hypothetical_protein_1686416..1686566_POSITIVE 567 50 1 75 25 tfor:TF0537|3end_conserved_hypothetical_protein_566836..566986_POSITIVE 543 128 83 84 44 tfor:TF2639|3end_hypothetical_protein_2809333..2809483_POSITIVE 512 55 12 79 36 tfor:TF0541|3end_conserved_hypothetical_protein_571858..572008_POSITIVE